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The map label for this gene is hybC [H]

Identifier: 116625968

GI number: 116625968

Start: 8698385

End: 8700085

Strand: Direct

Name: hybC [H]

Synonym: Acid_6926

Alternate gene names: 116625968

Gene position: 8698385-8700085 (Clockwise)

Preceding gene: 116625967

Following gene: 116625969

Centisome position: 87.28

GC content: 61.79

Gene sequence:

>1701_bases
ATGAGCAAGCGAATCGTCATCGACCCGGTAACCCGCATCGAGGGTCATTTACGCATCGATGTGGAAGTAGACGCCGGGCG
CGTCAGCGGCGCCTGGGCTTCGGGCACCATGTGGCGTGGGATCGAGCAGATCCTGCTGGGCCGCGATCCCCGCGAGGCGT
GGGTATTCGCGCAGCGTTTCTGCGGAGTCTGCACCACGGTACACGCCATTTCTTCCGTGCGCGCCGTGGAAGATGCGCTT
GGCCTCGTGATTCCGGCGAACGCTCAGTACATCCGGAATCTCATCCTGATCGCCCACGCGCTGCACGACCACATCGTGCA
TTTCTACCACCTTTGCGCGCTGGATTGGGTGGATGTGGTCTCCGCCTTGAAGGCCGACCCCGCCAAGAGCTCGTCGCTTG
CGGAATCCCTGTCACCCTGGATGCATAACTCAAAGAATGAACTGAAGGGCGTGCAGGATAAGTTGAAGGGCTTCGTCGAA
GCCCGCCAGCTGGGCATGTTCTCGAACGGCTACTGGGGCCACCCGGCGATGCACCTGGCGCCGGAAATCAACCTTGTCGC
CGTCTCCCATTACCTGCAGGCGCTCGAATACCAGCGCAAGGCGAATCAGATCGTCGCCATCCTCGGCAGCAAGACTCCCA
ACATTCAGAATCTCGCGGTAGGCGGCGTGGCCAATGCCATCAATCTGGATAACCAGGCCACACTGAACATGGAGAAGCTC
CTGATGATCCGCGGTCTCTTCGCCGAAGTGAACGCGTTCGTGCAACAGGTCTATCTGCCGGATGTCTGCGCCATCGGCGC
GATGTATCCGGAGTGGCTTAAGTTCGGCGCGGGCGTGATGAATTACCTCGCAATCCCGGATCTTCCTTTGGACGCCGAAG
CTACCAGGTTCGACCTTCCCGGCGGCATCATCATGGACGGCGCGCTGGCCGGCGTAACTCCCATCGCGAGCCATCACGAT
GAACGGCTTCGCAAGGGCGTTACGGAGAACGTGGCGCGGGCATTCTATAAGGGCAAGGAAACGCATCATCCGTGGGAAGG
CGAGACCATTCCCGACTACACGGAATACAGCGCCGAGGGCAAGTACTCCTGGGTCAAGGCGCCGCGCTTTGAAGGCAAAC
CGATGCAGGTGGGCCCGCTCGCGCAGGTGCTGGTGGGCTATGCCAGCGGCCACGCCCTTACCAGGAAATGGGCGGATCTC
GCACTGGGCACAATCTCTTCTATCGCCAAGACTCAAGTCACCCCCGCGGCACTGCATTCCACACTCGGCCGCGACGCCGC
CCGCGCCATCCGCGCAGCCATGCTCAGCGATCTCGCGATGAAGCACTGGGACCTGCTGGTGCAGAACATCGGCAAGGGCG
ACACCGATATCTTCGTGAAGCCCAACTTCCCCAAGGGCGAAATCCGCGGCGCGGGATTCCACGAAGCTCCGCGCGGCACG
CTCTCCCACTGGGCCGTGGTCACCGACGGCAAGATCACGAACTACCAGGCGGTGGTTCCAACCACCTGGAATGCCAGCCC
GCGCGATGAAAAGAACATCCCCGGCCCCTACGAGGCGTCTCTCAACGGCAACCCCATAGCGGACGCCGAGAAGCCCCTTG
AGGTTCTGCGCACGGTTCACTCGTTCGACCCCTGCCTGGCATGCGCCGTGCATACTCTCGATCCCGAGGGCCGCGAGTTG
GCGCGGATTAAGGTTCTATGA

Upstream 100 bases:

>100_bases
GCTTTCTGTTCACGGTCAAGAAGTTTCCGATTCTTCCCGTACCCATGAATCTCTACCTGAAATCGACGCAGACAAACGAT
TGGAATAAGGAGCACGCCGC

Downstream 100 bases:

>100_bases
CGGGCATCACTCTGCTTGGCATGGGTAACGTTCTGATGGGCGACGATGGCTTCGGACCCTACGTCATCGCTCTTCTGCGC
GCCCGATACGAGTTCGGCGC

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MSKRIVIDPVTRIEGHLRIDVEVDAGRVSGAWASGTMWRGIEQILLGRDPREAWVFAQRFCGVCTTVHAISSVRAVEDAL
GLVIPANAQYIRNLILIAHALHDHIVHFYHLCALDWVDVVSALKADPAKSSSLAESLSPWMHNSKNELKGVQDKLKGFVE
ARQLGMFSNGYWGHPAMHLAPEINLVAVSHYLQALEYQRKANQIVAILGSKTPNIQNLAVGGVANAINLDNQATLNMEKL
LMIRGLFAEVNAFVQQVYLPDVCAIGAMYPEWLKFGAGVMNYLAIPDLPLDAEATRFDLPGGIIMDGALAGVTPIASHHD
ERLRKGVTENVARAFYKGKETHHPWEGETIPDYTEYSAEGKYSWVKAPRFEGKPMQVGPLAQVLVGYASGHALTRKWADL
ALGTISSIAKTQVTPAALHSTLGRDAARAIRAAMLSDLAMKHWDLLVQNIGKGDTDIFVKPNFPKGEIRGAGFHEAPRGT
LSHWAVVTDGKITNYQAVVPTTWNASPRDEKNIPGPYEASLNGNPIADAEKPLEVLRTVHSFDPCLACAVHTLDPEGREL
ARIKVL

Sequences:

>Translated_566_residues
MSKRIVIDPVTRIEGHLRIDVEVDAGRVSGAWASGTMWRGIEQILLGRDPREAWVFAQRFCGVCTTVHAISSVRAVEDAL
GLVIPANAQYIRNLILIAHALHDHIVHFYHLCALDWVDVVSALKADPAKSSSLAESLSPWMHNSKNELKGVQDKLKGFVE
ARQLGMFSNGYWGHPAMHLAPEINLVAVSHYLQALEYQRKANQIVAILGSKTPNIQNLAVGGVANAINLDNQATLNMEKL
LMIRGLFAEVNAFVQQVYLPDVCAIGAMYPEWLKFGAGVMNYLAIPDLPLDAEATRFDLPGGIIMDGALAGVTPIASHHD
ERLRKGVTENVARAFYKGKETHHPWEGETIPDYTEYSAEGKYSWVKAPRFEGKPMQVGPLAQVLVGYASGHALTRKWADL
ALGTISSIAKTQVTPAALHSTLGRDAARAIRAAMLSDLAMKHWDLLVQNIGKGDTDIFVKPNFPKGEIRGAGFHEAPRGT
LSHWAVVTDGKITNYQAVVPTTWNASPRDEKNIPGPYEASLNGNPIADAEKPLEVLRTVHSFDPCLACAVHTLDPEGREL
ARIKVL
>Mature_565_residues
SKRIVIDPVTRIEGHLRIDVEVDAGRVSGAWASGTMWRGIEQILLGRDPREAWVFAQRFCGVCTTVHAISSVRAVEDALG
LVIPANAQYIRNLILIAHALHDHIVHFYHLCALDWVDVVSALKADPAKSSSLAESLSPWMHNSKNELKGVQDKLKGFVEA
RQLGMFSNGYWGHPAMHLAPEINLVAVSHYLQALEYQRKANQIVAILGSKTPNIQNLAVGGVANAINLDNQATLNMEKLL
MIRGLFAEVNAFVQQVYLPDVCAIGAMYPEWLKFGAGVMNYLAIPDLPLDAEATRFDLPGGIIMDGALAGVTPIASHHDE
RLRKGVTENVARAFYKGKETHHPWEGETIPDYTEYSAEGKYSWVKAPRFEGKPMQVGPLAQVLVGYASGHALTRKWADLA
LGTISSIAKTQVTPAALHSTLGRDAARAIRAAMLSDLAMKHWDLLVQNIGKGDTDIFVKPNFPKGEIRGAGFHEAPRGTL
SHWAVVTDGKITNYQAVVPTTWNASPRDEKNIPGPYEASLNGNPIADAEKPLEVLRTVHSFDPCLACAVHTLDPEGRELA
RIKVL

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=567, Percent_Identity=56.0846560846561, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1787207, Length=592, Percent_Identity=43.4121621621622, Blast_Score=477, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 61936; Mature: 61805

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRIVIDPVTRIEGHLRIDVEVDAGRVSGAWASGTMWRGIEQILLGRDPREAWVFAQRF
CCCCEEECCHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
CGVCTTVHAISSVRAVEDALGLVIPANAQYIRNLILIAHALHDHIVHFYHLCALDWVDVV
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALKADPAKSSSLAESLSPWMHNSKNELKGVQDKLKGFVEARQLGMFSNGYWGHPAMHLA
HHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHC
PEINLVAVSHYLQALEYQRKANQIVAILGSKTPNIQNLAVGGVANAINLDNQATLNMEKL
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCHHHCCCCCCCCCCHHHH
LMIRGLFAEVNAFVQQVYLPDVCAIGAMYPEWLKFGAGVMNYLAIPDLPLDAEATRFDLP
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
GGIIMDGALAGVTPIASHHDERLRKGVTENVARAFYKGKETHHPWEGETIPDYTEYSAEG
CCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCC
KYSWVKAPRFEGKPMQVGPLAQVLVGYASGHALTRKWADLALGTISSIAKTQVTPAALHS
CEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
TLGRDAARAIRAAMLSDLAMKHWDLLVQNIGKGDTDIFVKPNFPKGEIRGAGFHEAPRGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LSHWAVVTDGKITNYQAVVPTTWNASPRDEKNIPGPYEASLNGNPIADAEKPLEVLRTVH
CCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SFDPCLACAVHTLDPEGRELARIKVL
CCCHHHHHHHHCCCCCCCCEEEEECC
>Mature Secondary Structure 
SKRIVIDPVTRIEGHLRIDVEVDAGRVSGAWASGTMWRGIEQILLGRDPREAWVFAQRF
CCCEEECCHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
CGVCTTVHAISSVRAVEDALGLVIPANAQYIRNLILIAHALHDHIVHFYHLCALDWVDVV
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALKADPAKSSSLAESLSPWMHNSKNELKGVQDKLKGFVEARQLGMFSNGYWGHPAMHLA
HHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHC
PEINLVAVSHYLQALEYQRKANQIVAILGSKTPNIQNLAVGGVANAINLDNQATLNMEKL
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCHHHCCCCCCCCCCHHHH
LMIRGLFAEVNAFVQQVYLPDVCAIGAMYPEWLKFGAGVMNYLAIPDLPLDAEATRFDLP
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
GGIIMDGALAGVTPIASHHDERLRKGVTENVARAFYKGKETHHPWEGETIPDYTEYSAEG
CCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCC
KYSWVKAPRFEGKPMQVGPLAQVLVGYASGHALTRKWADLALGTISSIAKTQVTPAALHS
CEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
TLGRDAARAIRAAMLSDLAMKHWDLLVQNIGKGDTDIFVKPNFPKGEIRGAGFHEAPRGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LSHWAVVTDGKITNYQAVVPTTWNASPRDEKNIPGPYEASLNGNPIADAEKPLEVLRTVH
CCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SFDPCLACAVHTLDPEGRELARIKVL
CCCHHHHHHHHCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]