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The map label for this gene is hybD [H]

Identifier: 116625969

GI number: 116625969

Start: 8700082

End: 8700615

Strand: Direct

Name: hybD [H]

Synonym: Acid_6927

Alternate gene names: 116625969

Gene position: 8700082-8700615 (Clockwise)

Preceding gene: 116625968

Following gene: 116625970

Centisome position: 87.3

GC content: 64.23

Gene sequence:

>534_bases
ATGACGGGCATCACTCTGCTTGGCATGGGTAACGTTCTGATGGGCGACGATGGCTTCGGACCCTACGTCATCGCTCTTCT
GCGCGCCCGATACGAGTTCGGCGCCAATTTGGAGGTGTTGGATATCGGCACCCCCGGCCTCGATATCACGCCCTATCTGG
CCCGTCCCGGGCGTAGCGTGATCCTGATCGATACCGTGAGGAGCGACGGCCCCGCGGGAACATTGCGCCGCTACACCGGC
CCGCAAATCATGGGCGGGCGCATGCAACCGCGCCTGAGTCCGCATGATCCGGCCCTCGCCGAAGCCCTCACCATGCTGGA
AATTGAAGGCACGGCGCCGGCCGAGTTCCTGCTGCTCGGAGCCGTTCCCGAAAAATGCGCCATGGGCACGGGGCTCAGTT
CTGCCATGCAGTCGGCGGTTGAGCCGATGATCGCCTCTGTACTCTCCGCGCTGGCGAATCGGGGTGTCGAAATCCGCGAA
CGCGAAACCCCTGGCGCGCCCGACCTCTGGTGGGAAGCCCCGCTTCTGGTTTGA

Upstream 100 bases:

>100_bases
TTGAGGTTCTGCGCACGGTTCACTCGTTCGACCCCTGCCTGGCATGCGCCGTGCATACTCTCGATCCCGAGGGCCGCGAG
TTGGCGCGGATTAAGGTTCT

Downstream 100 bases:

>100_bases
TCAACTGCGCCAGATCACCCAACCAGGAGAGCCGCTTCTAGTGCGGGTCCGGTCCGCAGGGCCCGTGGTCTGAGACATAA
GCATTCTCGTTTCAACCATC

Product: hydrogenase maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MTGITLLGMGNVLMGDDGFGPYVIALLRARYEFGANLEVLDIGTPGLDITPYLARPGRSVILIDTVRSDGPAGTLRRYTG
PQIMGGRMQPRLSPHDPALAEALTMLEIEGTAPAEFLLLGAVPEKCAMGTGLSSAMQSAVEPMIASVLSALANRGVEIRE
RETPGAPDLWWEAPLLV

Sequences:

>Translated_177_residues
MTGITLLGMGNVLMGDDGFGPYVIALLRARYEFGANLEVLDIGTPGLDITPYLARPGRSVILIDTVRSDGPAGTLRRYTG
PQIMGGRMQPRLSPHDPALAEALTMLEIEGTAPAEFLLLGAVPEKCAMGTGLSSAMQSAVEPMIASVLSALANRGVEIRE
RETPGAPDLWWEAPLLV
>Mature_176_residues
TGITLLGMGNVLMGDDGFGPYVIALLRARYEFGANLEVLDIGTPGLDITPYLARPGRSVILIDTVRSDGPAGTLRRYTGP
QIMGGRMQPRLSPHDPALAEALTMLEIEGTAPAEFLLLGAVPEKCAMGTGLSSAMQSAVEPMIASVLSALANRGVEIRER
ETPGAPDLWWEAPLLV

Specific function: Protease involved in the C-terminal processing of hybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of hybC [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=162, Percent_Identity=31.4814814814815, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787209, Length=120, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 18758; Mature: 18627

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGITLLGMGNVLMGDDGFGPYVIALLRARYEFGANLEVLDIGTPGLDITPYLARPGRSV
CCCEEEEECCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCEE
ILIDTVRSDGPAGTLRRYTGPQIMGGRMQPRLSPHDPALAEALTMLEIEGTAPAEFLLLG
EEEEECCCCCCCCCHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHEEECCCCCCEEEEEE
AVPEKCAMGTGLSSAMQSAVEPMIASVLSALANRGVEIRERETPGAPDLWWEAPLLV
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGITLLGMGNVLMGDDGFGPYVIALLRARYEFGANLEVLDIGTPGLDITPYLARPGRSV
CCEEEEECCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCEE
ILIDTVRSDGPAGTLRRYTGPQIMGGRMQPRLSPHDPALAEALTMLEIEGTAPAEFLLLG
EEEEECCCCCCCCCHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHEEECCCCCCEEEEEE
AVPEKCAMGTGLSSAMQSAVEPMIASVLSALANRGVEIRERETPGAPDLWWEAPLLV
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8021226; 9278503; 10331925 [H]