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The map label for this gene is hybO [H]

Identifier: 116625965

GI number: 116625965

Start: 8695040

End: 8696188

Strand: Direct

Name: hybO [H]

Synonym: Acid_6923

Alternate gene names: 116625965

Gene position: 8695040-8696188 (Clockwise)

Preceding gene: 116625958

Following gene: 116625966

Centisome position: 87.25

GC content: 62.32

Gene sequence:

>1149_bases
ATGCCCCAGTCGCCTACATCCTGCTCGCCCCCAACAGACAGGCACACTCAATCCCCTGGAATGGACCGGCGTTCGTTCTT
TAAGGCGGTTGCCCAAATTGCCGCTTCGGTTGGCTTGTCCGCCGCGACCGCCAACCGTATCGCCGAGGCCGCGGTCCGGG
GCAAGAAGCCAACCGTGATCTGGCTGCACTTTCAGGATTGCACGGGCTGCACGGAGTCTCTCCTCAGGAGCAGCCAGCCC
GGGTTCGCCGAACTCGTGCTGGATCTCATCTCGCTCGATTACCACGAGACCGTAATGGCCGCGGCGGGCCACCAGGCCGA
AGAGGCGCTGAACGCGTCAATCAAAACCAATGCCGGGAAGTACATTCTGGTAGTCGAAGGCGCCATTCCTCTGGCGAACA
ACGGCAACTTTATCCGTATCGCAGGACGCACCGGGGTGCAGATGCTGAAGGACGTGGCGGCCGGTGCCGGCCTGGTGATC
GCGATCGGATCCTGCGCGTCCTGGGGCGGCCTGCCTTCCGCCGACCCAAACCCAACCGGGGCGGTTGGCGCGCACCACGT
GCTCACCGGGAGCAGGATCGTCAACCTTCCTGGTTGTCCAGCCAATCCGAATATCTTCCTGGCTACCGCGCTGCAGTTCG
CGACGTTGGGGACGCTTCCCGAACTGGACGCCGAGAATCGCCCTAAATTCGCATACGGCCGTCTCATCCACGATCACTGT
CCGCGGCGAGCGCATTTTGATGCGGGCCGCTTCGCCGATCAATTCGGCGACGCCGGCCATCGGCTCGGCCATTGTCTTTA
TAAGTTGGGCTGCAAAGGCCCGGCAACGCACGCCAACTGCAGTACAAATTCCTTCTGCGAAGTAGTTGATGCGTGGCCGA
TCGGCCTGGGCCATCCCTGCTTCGGCTGCACCGAAAAGAGCCTCGCTTTTCGAGTGCCGATGTTCCAAACGGTGAACATA
GAGCGCGTAAAGCCACCCGAATTCTATCCAGGTCTCACGGCGCACTATAGCAGCGTCAGCGCGGCCGCCACCGGCCTCGC
CGGATTGGTCGCCGGCGGCGCGATCGCCGGAGCCTTGAGGCTGTCCAAGAAGGTGGAAGAGACCGCCGCTGAAACAGATT
CGAATCCTCCAGACGGAGGAGCCAGGTGA

Upstream 100 bases:

>100_bases
CATGGGCCAACAGGAAACGTTGAAAACAAAAGCCTGACTATTAAAATCCCTCTGGGCCGTTCGATTGCATTGCAGCAACC
CAGTATCTGAGGAGACGCAC

Downstream 100 bases:

>100_bases
GCGCGGAGAGCCAGGGTATCGACCGCCGCGGGCTGTTTCGCATCCTGGCGGCCGGCGCGGCCACTGCCGCAGTTGCCGTG
CCGCCCGCCCAAGCCCGCGT

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 small subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MPQSPTSCSPPTDRHTQSPGMDRRSFFKAVAQIAASVGLSAATANRIAEAAVRGKKPTVIWLHFQDCTGCTESLLRSSQP
GFAELVLDLISLDYHETVMAAAGHQAEEALNASIKTNAGKYILVVEGAIPLANNGNFIRIAGRTGVQMLKDVAAGAGLVI
AIGSCASWGGLPSADPNPTGAVGAHHVLTGSRIVNLPGCPANPNIFLATALQFATLGTLPELDAENRPKFAYGRLIHDHC
PRRAHFDAGRFADQFGDAGHRLGHCLYKLGCKGPATHANCSTNSFCEVVDAWPIGLGHPCFGCTEKSLAFRVPMFQTVNI
ERVKPPEFYPGLTAHYSSVSAAATGLAGLVAGGAIAGALRLSKKVEETAAETDSNPPDGGAR

Sequences:

>Translated_382_residues
MPQSPTSCSPPTDRHTQSPGMDRRSFFKAVAQIAASVGLSAATANRIAEAAVRGKKPTVIWLHFQDCTGCTESLLRSSQP
GFAELVLDLISLDYHETVMAAAGHQAEEALNASIKTNAGKYILVVEGAIPLANNGNFIRIAGRTGVQMLKDVAAGAGLVI
AIGSCASWGGLPSADPNPTGAVGAHHVLTGSRIVNLPGCPANPNIFLATALQFATLGTLPELDAENRPKFAYGRLIHDHC
PRRAHFDAGRFADQFGDAGHRLGHCLYKLGCKGPATHANCSTNSFCEVVDAWPIGLGHPCFGCTEKSLAFRVPMFQTVNI
ERVKPPEFYPGLTAHYSSVSAAATGLAGLVAGGAIAGALRLSKKVEETAAETDSNPPDGGAR
>Mature_381_residues
PQSPTSCSPPTDRHTQSPGMDRRSFFKAVAQIAASVGLSAATANRIAEAAVRGKKPTVIWLHFQDCTGCTESLLRSSQPG
FAELVLDLISLDYHETVMAAAGHQAEEALNASIKTNAGKYILVVEGAIPLANNGNFIRIAGRTGVQMLKDVAAGAGLVIA
IGSCASWGGLPSADPNPTGAVGAHHVLTGSRIVNLPGCPANPNIFLATALQFATLGTLPELDAENRPKFAYGRLIHDHCP
RRAHFDAGRFADQFGDAGHRLGHCLYKLGCKGPATHANCSTNSFCEVVDAWPIGLGHPCFGCTEKSLAFRVPMFQTVNIE
RVKPPEFYPGLTAHYSSVSAAATGLAGLVAGGAIAGALRLSKKVEETAAETDSNPPDGGAR

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side. Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=328, Percent_Identity=50.609756097561, Blast_Score=335, Evalue=2e-93,
Organism=Escherichia coli, GI1787206, Length=299, Percent_Identity=45.1505016722408, Blast_Score=248, Evalue=7e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 39944; Mature: 39812

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSPTSCSPPTDRHTQSPGMDRRSFFKAVAQIAASVGLSAATANRIAEAAVRGKKPTVI
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
WLHFQDCTGCTESLLRSSQPGFAELVLDLISLDYHETVMAAAGHQAEEALNASIKTNAGK
EEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHCCCEECCCCC
YILVVEGAIPLANNGNFIRIAGRTGVQMLKDVAAGAGLVIAIGSCASWGGLPSADPNPTG
EEEEEECCEEECCCCCEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCC
AVGAHHVLTGSRIVNLPGCPANPNIFLATALQFATLGTLPELDAENRPKFAYGRLIHDHC
CCCCCEEECCCEEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHC
PRRAHFDAGRFADQFGDAGHRLGHCLYKLGCKGPATHANCSTNSFCEVVDAWPIGLGHPC
CCCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
FGCTEKSLAFRVPMFQTVNIERVKPPEFYPGLTAHYSSVSAAATGLAGLVAGGAIAGALR
CCCCCCCCEEECCCEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKKVEETAAETDSNPPDGGAR
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PQSPTSCSPPTDRHTQSPGMDRRSFFKAVAQIAASVGLSAATANRIAEAAVRGKKPTVI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEE
WLHFQDCTGCTESLLRSSQPGFAELVLDLISLDYHETVMAAAGHQAEEALNASIKTNAGK
EEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHCCCEECCCCC
YILVVEGAIPLANNGNFIRIAGRTGVQMLKDVAAGAGLVIAIGSCASWGGLPSADPNPTG
EEEEEECCEEECCCCCEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCC
AVGAHHVLTGSRIVNLPGCPANPNIFLATALQFATLGTLPELDAENRPKFAYGRLIHDHC
CCCCCEEECCCEEEECCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHC
PRRAHFDAGRFADQFGDAGHRLGHCLYKLGCKGPATHANCSTNSFCEVVDAWPIGLGHPC
CCCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
FGCTEKSLAFRVPMFQTVNIERVKPPEFYPGLTAHYSSVSAAATGLAGLVAGGAIAGALR
CCCCCCCCEEECCCEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKKVEETAAETDSNPPDGGAR
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]