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The map label for this gene is hybA [H]

Identifier: 116625966

GI number: 116625966

Start: 8696185

End: 8697138

Strand: Direct

Name: hybA [H]

Synonym: Acid_6924

Alternate gene names: 116625966

Gene position: 8696185-8697138 (Clockwise)

Preceding gene: 116625965

Following gene: 116625967

Centisome position: 87.26

GC content: 60.8

Gene sequence:

>954_bases
GTGAGCGCGGAGAGCCAGGGTATCGACCGCCGCGGGCTGTTTCGCATCCTGGCGGCCGGCGCGGCCACTGCCGCAGTTGC
CGTGCCGCCCGCCCAAGCCCGCGTAGCGCCGGAGGAAGTCCGTGAGGCGCCGCAGAATGCCGCGTCCATGCTTTATGACA
CGACGCTCTGCATTGGCTGTAAAGCCTGCGTAGGCGCCTGCAATGAGGCCAACGGACTGCTCCCCGATCCGGGATTCTCA
AAGGCCTTCGGAAACATCTACGATGCGCCCCCGTCGCTCAATGCCTCGACGAAGAACATCATCAAGCTGTACAAGAGCCG
GACCAGCGACGAATGGTCCTTCGTCAAGCAGCAATGCATGCACTGCGTTGACCCCTCCTGCGTACAGGCGTGCCCGCTCT
CGGCGCTCACCAAGGGAGATCACGGCATCGTGGCCTGGAACGGCAATCAATGCATCGGCTGCCGGTGCTGCCAGCTCTCC
TGCCCTTTCAATATTCCTAAGTTCGAGTGGAGCAGTTTCAATCCTAAGATTGTGAAATGCGAGTTCTGCCGGCACCTCCT
GGCGGTTGGCAAGAACCCGGCTTGCACGTCGGCGTGTCCACGCGGGGCAGTGATTTACGGCACTCGCGAACAGCTCATGG
CGGAAGCCAAAAACCGGATCGCGAAATCTCCCGGCAAGTACTTTGAGAACCGCGTCTACGGGGAAAAGGAAGGCGGCGGC
ACACAGGTGCTTTACCTTTCGCACGTGGCATTCAGCCAGATCGGCCTGCCCGACATGGAAGACGAAGCCAACGCCGCCAG
AGTCCGTAGCCATTCGGCGGCGATCTACCACGGCGGCGTCACGCCCCTGGTGATTTACGGCGTTCTGCTTGGGTTTACAC
GGCACTTCTTTAAGAAGCACCTCGAGGAAGCCGAACAGGAAAGAGCCGAAACGGGCCTCAAGGAGCAACTCTAA

Upstream 100 bases:

>100_bases
TGGTCGCCGGCGGCGCGATCGCCGGAGCCTTGAGGCTGTCCAAGAAGGTGGAAGAGACCGCCGCTGAAACAGATTCGAAT
CCTCCAGACGGAGGAGCCAG

Downstream 100 bases:

>100_bases
TGCGATGGAGTTCGACTTACCCCAACGCCTGGCGCCGGGCCATCGTTTTGGGCGGCCGCGTGATCACGTTGCCGTTTGTT
GTAGTCTCCGCGATCGCGGC

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSAESQGIDRRGLFRILAAGAATAAVAVPPAQARVAPEEVREAPQNAASMLYDTTLCIGCKACVGACNEANGLLPDPGFS
KAFGNIYDAPPSLNASTKNIIKLYKSRTSDEWSFVKQQCMHCVDPSCVQACPLSALTKGDHGIVAWNGNQCIGCRCCQLS
CPFNIPKFEWSSFNPKIVKCEFCRHLLAVGKNPACTSACPRGAVIYGTREQLMAEAKNRIAKSPGKYFENRVYGEKEGGG
TQVLYLSHVAFSQIGLPDMEDEANAARVRSHSAAIYHGGVTPLVIYGVLLGFTRHFFKKHLEEAEQERAETGLKEQL

Sequences:

>Translated_317_residues
MSAESQGIDRRGLFRILAAGAATAAVAVPPAQARVAPEEVREAPQNAASMLYDTTLCIGCKACVGACNEANGLLPDPGFS
KAFGNIYDAPPSLNASTKNIIKLYKSRTSDEWSFVKQQCMHCVDPSCVQACPLSALTKGDHGIVAWNGNQCIGCRCCQLS
CPFNIPKFEWSSFNPKIVKCEFCRHLLAVGKNPACTSACPRGAVIYGTREQLMAEAKNRIAKSPGKYFENRVYGEKEGGG
TQVLYLSHVAFSQIGLPDMEDEANAARVRSHSAAIYHGGVTPLVIYGVLLGFTRHFFKKHLEEAEQERAETGLKEQL
>Mature_316_residues
SAESQGIDRRGLFRILAAGAATAAVAVPPAQARVAPEEVREAPQNAASMLYDTTLCIGCKACVGACNEANGLLPDPGFSK
AFGNIYDAPPSLNASTKNIIKLYKSRTSDEWSFVKQQCMHCVDPSCVQACPLSALTKGDHGIVAWNGNQCIGCRCCQLSC
PFNIPKFEWSSFNPKIVKCEFCRHLLAVGKNPACTSACPRGAVIYGTREQLMAEAKNRIAKSPGKYFENRVYGEKEGGGT
QVLYLSHVAFSQIGLPDMEDEANAARVRSHSAAIYHGGVTPLVIYGVLLGFTRHFFKKHLEEAEQERAETGLKEQL

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=324, Percent_Identity=36.1111111111111, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787749, Length=321, Percent_Identity=30.8411214953271, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1790326, Length=304, Percent_Identity=33.2236842105263, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787122, Length=176, Percent_Identity=28.9772727272727, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787872, Length=176, Percent_Identity=29.5454545454545, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI226510944, Length=200, Percent_Identity=30, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI2367345, Length=177, Percent_Identity=31.0734463276836, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI2367245, Length=182, Percent_Identity=26.9230769230769, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082179, Length=176, Percent_Identity=27.8409090909091, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 34353; Mature: 34221

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAESQGIDRRGLFRILAAGAATAAVAVPPAQARVAPEEVREAPQNAASMLYDTTLCIGC
CCCCCCCCCHHHHHHHHHHHHHHHEEECCCHHHCCCHHHHHHCHHHHHHHHHHHHHHHHH
KACVGACNEANGLLPDPGFSKAFGNIYDAPPSLNASTKNIIKLYKSRTSDEWSFVKQQCM
HHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
HCVDPSCVQACPLSALTKGDHGIVAWNGNQCIGCRCCQLSCPFNIPKFEWSSFNPKIVKC
HHCCHHHHHHCCHHHHCCCCCEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEEH
EFCRHLLAVGKNPACTSACPRGAVIYGTREQLMAEAKNRIAKSPGKYFENRVYGEKEGGG
HHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
TQVLYLSHVAFSQIGLPDMEDEANAARVRSHSAAIYHGGVTPLVIYGVLLGFTRHFFKKH
EEEEEEEHHHHHHCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
LEEAEQERAETGLKEQL
HHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
SAESQGIDRRGLFRILAAGAATAAVAVPPAQARVAPEEVREAPQNAASMLYDTTLCIGC
CCCCCCCCHHHHHHHHHHHHHHHEEECCCHHHCCCHHHHHHCHHHHHHHHHHHHHHHHH
KACVGACNEANGLLPDPGFSKAFGNIYDAPPSLNASTKNIIKLYKSRTSDEWSFVKQQCM
HHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
HCVDPSCVQACPLSALTKGDHGIVAWNGNQCIGCRCCQLSCPFNIPKFEWSSFNPKIVKC
HHCCHHHHHHCCHHHHCCCCCEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEEH
EFCRHLLAVGKNPACTSACPRGAVIYGTREQLMAEAKNRIAKSPGKYFENRVYGEKEGGG
HHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
TQVLYLSHVAFSQIGLPDMEDEANAARVRSHSAAIYHGGVTPLVIYGVLLGFTRHFFKKH
EEEEEEEHHHHHHCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
LEEAEQERAETGLKEQL
HHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]