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The map label for this gene is purN [H]

Identifier: 116625773

GI number: 116625773

Start: 8424551

End: 8425150

Strand: Direct

Name: purN [H]

Synonym: Acid_6723

Alternate gene names: 116625773

Gene position: 8424551-8425150 (Clockwise)

Preceding gene: 116625772

Following gene: 116625777

Centisome position: 84.54

GC content: 65.5

Gene sequence:

>600_bases
GTGAAGCGACTCGGCATTCTGATCTCCGGCCGGGGATCGAATTTCGAGGCGATTGCCGCGAACGTGCAGAGCGGCGCGCT
GAACGCCGATATCGCGGTGGTGATCGCCAACCGTGCGGAGGCTCCGGGCCTGGAGATCGCGCGGGCCCGCGGCTTGACTG
CCGTCTGCCTGCCTTCGAAGGGCCTGGATCGCGAAGTCTATGACCGGATGCTGGCCGCGGAGTTGCGCAGGCACGAGGTC
GAACTGGTCTGCCTCGCGGGCTTCATGCGGCTCCTCAGCGCCGGCTTCGTCCGCGAATTTCCGCAGAGGATTTTGAACAT
CCACCCCTCGCTGCTGCCGGCCTTTCCCGGCCTGGACGCGCAGCATCAGGCGCTGGCGCACGGCGTGAAGCTGACGGGGT
GCACCGTCCATTTCGTGGACCAGGACCTCGATGCGGGCCCGATTGTCTTACAGGCGGCCGTCCCGGTGAAGGACGATGAC
ACGGTGGATGCGCTCTCGGCGCGCATCCTCAAAGAGGAGCACCGCATTTATTCGGAGGCGATCCGCATTGTGATCGCGGG
GAACTACCGGATCGACGGGCGGCGGGTTCTACTCGGTTGA

Upstream 100 bases:

>100_bases
CGCTTAAGGCCCAGGCCGCACTGCGCAAGATCGGCGAGACGCCCTTCCGGATCGGCAGCGTGGTGGAACTCAAGCGCGGC
CGTCCGCGCGTGGAGTACCA

Downstream 100 bases:

>100_bases
CTCCGCGGCCCGGCGGCGCTCGCGGAGAGCCAGAAAGTGCGAGAGCAGCATGGGCGGTCCGGGACCTGCATCCAGTTGGC
GTTCCACGGCCCGCTGATAC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKRLGILISGRGSNFEAIAANVQSGALNADIAVVIANRAEAPGLEIARARGLTAVCLPSKGLDREVYDRMLAAELRRHEV
ELVCLAGFMRLLSAGFVREFPQRILNIHPSLLPAFPGLDAQHQALAHGVKLTGCTVHFVDQDLDAGPIVLQAAVPVKDDD
TVDALSARILKEEHRIYSEAIRIVIAGNYRIDGRRVLLG

Sequences:

>Translated_199_residues
MKRLGILISGRGSNFEAIAANVQSGALNADIAVVIANRAEAPGLEIARARGLTAVCLPSKGLDREVYDRMLAAELRRHEV
ELVCLAGFMRLLSAGFVREFPQRILNIHPSLLPAFPGLDAQHQALAHGVKLTGCTVHFVDQDLDAGPIVLQAAVPVKDDD
TVDALSARILKEEHRIYSEAIRIVIAGNYRIDGRRVLLG
>Mature_199_residues
MKRLGILISGRGSNFEAIAANVQSGALNADIAVVIANRAEAPGLEIARARGLTAVCLPSKGLDREVYDRMLAAELRRHEV
ELVCLAGFMRLLSAGFVREFPQRILNIHPSLLPAFPGLDAQHQALAHGVKLTGCTVHFVDQDLDAGPIVLQAAVPVKDDD
TVDALSARILKEEHRIYSEAIRIVIAGNYRIDGRRVLLG

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=44.2105263157895, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=44.2105263157895, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=44.2105263157895, Blast_Score=162, Evalue=2e-40,
Organism=Escherichia coli, GI1788846, Length=199, Percent_Identity=42.2110552763819, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1787483, Length=197, Percent_Identity=30.9644670050761, Blast_Score=100, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17567511, Length=184, Percent_Identity=42.3913043478261, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6320616, Length=209, Percent_Identity=28.7081339712919, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24582400, Length=193, Percent_Identity=46.6321243523316, Blast_Score=171, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21545; Mature: 21545

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLGILISGRGSNFEAIAANVQSGALNADIAVVIANRAEAPGLEIARARGLTAVCLPSK
CCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHCCCEEEEECCC
GLDREVYDRMLAAELRRHEVELVCLAGFMRLLSAGFVREFPQRILNIHPSLLPAFPGLDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCH
QHQALAHGVKLTGCTVHFVDQDLDAGPIVLQAAVPVKDDDTVDALSARILKEEHRIYSEA
HHHHHHCCEEEECEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
IRIVIAGNYRIDGRRVLLG
EEEEEECCEEECCEEEECC
>Mature Secondary Structure
MKRLGILISGRGSNFEAIAANVQSGALNADIAVVIANRAEAPGLEIARARGLTAVCLPSK
CCCEEEEEECCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHCCCEEEEECCC
GLDREVYDRMLAAELRRHEVELVCLAGFMRLLSAGFVREFPQRILNIHPSLLPAFPGLDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCH
QHQALAHGVKLTGCTVHFVDQDLDAGPIVLQAAVPVKDDDTVDALSARILKEEHRIYSEA
HHHHHHCCEEEECEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
IRIVIAGNYRIDGRRVLLG
EEEEEECCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]