Click here to switch to the map view.

The map label for this gene is rnhA

Identifier: 116625731

GI number: 116625731

Start: 8382783

End: 8383223

Strand: Direct

Name: rnhA

Synonym: Acid_6680

Alternate gene names: 116625731

Gene position: 8382783-8383223 (Clockwise)

Preceding gene: 116625730

Following gene: 116625732

Centisome position: 84.12

GC content: 58.73

Gene sequence:

>441_bases
GTGAAAAAAGTTCAATTGATCACCGATGGCGCCTGCCTCGGCAACCCCGGTCCCGGCGGCTGGTCCGCCATCCTTCGTTT
TGAGGAACAGAAGAAGGAGTTGTGGGGCTGTGAGAAACAGACCACCAACAACCGAATGGAATTGACCGCCGCCATCGAAG
GCCTTCGCGCCTTGCGTGAAAAGTGCCAGGTCGAAGTGGTCACGGATTCCGAGTACGTCCTGAAGGGCATCACCACCTGG
ATCGACGGCTGGAAAAGGAAAGGCTGGATGACCGCCGCCAAGAAACCCGTCATCAACCAGGACCTCTGGAAGCTTCTCGA
CGAGCAGGTCAACCGGCACCAGGCCACCTGGACCTGGACCAAAGGTCACGCCAGCCATGCCGATAACAATCGCTGCGACG
AACTTGCCACTCGCGCCGCCCGGGAGCAGTCCAAAAGCTAA

Upstream 100 bases:

>100_bases
ACGGTGCCCACCTGCGCGCCTCCATCTATCAGGCCCACGAAACCGCCGCCATTCTTGAACTTGTCGACCAGTTCTTTCAC
TCCGAACCGGTGCTCGCTCA

Downstream 100 bases:

>100_bases
TTAGATGGATGCCCTAACCCTACACCGCCTGCACTTCGCCTTCACCATCACCTTTCACTATCTCTTTCCGCAATTGACCA
TGGGGCTCGCGCTCCTCATC

Product: RNase HI

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKKVQLITDGACLGNPGPGGWSAILRFEEQKKELWGCEKQTTNNRMELTAAIEGLRALREKCQVEVVTDSEYVLKGITTW
IDGWKRKGWMTAAKKPVINQDLWKLLDEQVNRHQATWTWTKGHASHADNNRCDELATRAAREQSKS

Sequences:

>Translated_146_residues
MKKVQLITDGACLGNPGPGGWSAILRFEEQKKELWGCEKQTTNNRMELTAAIEGLRALREKCQVEVVTDSEYVLKGITTW
IDGWKRKGWMTAAKKPVINQDLWKLLDEQVNRHQATWTWTKGHASHADNNRCDELATRAAREQSKS
>Mature_146_residues
MKKVQLITDGACLGNPGPGGWSAILRFEEQKKELWGCEKQTTNNRMELTAAIEGLRALREKCQVEVVTDSEYVLKGITTW
IDGWKRKGWMTAAKKPVINQDLWKLLDEQVNRHQATWTWTKGHASHADNNRCDELATRAAREQSKS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=103, Percent_Identity=32.0388349514563, Blast_Score=63, Evalue=7e-11,
Organism=Escherichia coli, GI1786408, Length=140, Percent_Identity=57.1428571428571, Blast_Score=172, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137476, Length=147, Percent_Identity=29.2517006802721, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=29.2517006802721, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_SOLUE (Q01RW8)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827887.1
- ProteinModelPortal:   Q01RW8
- STRING:   Q01RW8
- GeneID:   4425203
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_6680
- NMPDR:   fig|234267.9.peg.6350
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q01RW8
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16637; Mature: 16637

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVQLITDGACLGNPGPGGWSAILRFEEQKKELWGCEKQTTNNRMELTAAIEGLRALRE
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
KCQVEVVTDSEYVLKGITTWIDGWKRKGWMTAAKKPVINQDLWKLLDEQVNRHQATWTWT
HCEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
KGHASHADNNRCDELATRAAREQSKS
CCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKVQLITDGACLGNPGPGGWSAILRFEEQKKELWGCEKQTTNNRMELTAAIEGLRALRE
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
KCQVEVVTDSEYVLKGITTWIDGWKRKGWMTAAKKPVINQDLWKLLDEQVNRHQATWTWT
HCEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEE
KGHASHADNNRCDELATRAAREQSKS
CCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA