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The map label for this gene is ywkE [H]

Identifier: 116625720

GI number: 116625720

Start: 8372914

End: 8373732

Strand: Reverse

Name: ywkE [H]

Synonym: Acid_6669

Alternate gene names: 116625720

Gene position: 8373732-8372914 (Counterclockwise)

Preceding gene: 116625721

Following gene: 116625719

Centisome position: 84.03

GC content: 65.2

Gene sequence:

>819_bases
ATGAACGTTCAGACAGCGCTGGTGCAGGGGACGCGGCTGTTGGAGGATGCCGGGGTGGGCGTGCCGCGGCTCACGGCGGA
AGTGCTGCTGGGGCATGCGATGCGCGTGCAGCGGGAATACTTTTACGCGCATCCGGAGCAGGAATTGCGCGAGGTGGAAT
GGCTCCACTACGGCCGCTACCTGCACGAACGGCTCGGCGGCAAGCCGACGCAATACATCACACATCGACAGGAATTTTAC
AGCCGGGAATTCCGGGTCACGCCGGATGTTCTGATTCCGCGTCCGGAGACGGAGCATGTGGTGGAAGCGGCGCTTGAAGT
GGCACGGGGGGCAAAGCGCGTGCTCGATGTCGGCACCGGGTCGGGCGCGCTGGCGATCACGCTGCGACTGGAGATGGGCA
CGGAAGCCTGGGCCACGGACATTTCGGCAGAGGCCGCGACGGTAGCGGCAGGCAATGCAGCGCGGCTTGGGGCGAACGTG
CATTTCGCGGTCTGCGACCTGATGGATGCGATCGCGGCGGACTCCATGAATCTGATCGTTTCCAATCCACCCTATGTGCC
GCTGGCGCACCGCGAGGGGCTGCAGCGCGAGGTGCGCGACTTCGAACCGCACGTGGCGTTGTTCGGAGGGCAGACCGGAT
TTGAACTCTACGACAGGATCGTGGCGGACGCGCCGCGCGTACTGCGCCCCGGCGGCTGGTTGATCATGGAGTTGGGATTC
ACCAGCCTGGATCATGTGCGGGCGCTGCTGGCGAGTTGGAGCGACGTGCGCGTAATCCCCGACCTGGCCGGCATCCCGCG
GGTAATTGCCGCCCGATAA

Upstream 100 bases:

>100_bases
GCCGGCTGCCGGCAGGATTGCCTGCCCCACAGAATGGGCATGCCGCAAGGCGTGCCGCGAGATCGTTCCCTCACGGTCGT
GGCTCGTTGACGGAGAGCGA

Downstream 100 bases:

>100_bases
GATGGGCATATGCGTATTCAAACGGCGGCGCTGATTTTCTGGGCGTCCGCCTCTTCCTTATTTGCCGCGGATTGGAAGGT
TGCCGACAATCCTCTCTCGA

Product: HemK family modification methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNVQTALVQGTRLLEDAGVGVPRLTAEVLLGHAMRVQREYFYAHPEQELREVEWLHYGRYLHERLGGKPTQYITHRQEFY
SREFRVTPDVLIPRPETEHVVEAALEVARGAKRVLDVGTGSGALAITLRLEMGTEAWATDISAEAATVAAGNAARLGANV
HFAVCDLMDAIAADSMNLIVSNPPYVPLAHREGLQREVRDFEPHVALFGGQTGFELYDRIVADAPRVLRPGGWLIMELGF
TSLDHVRALLASWSDVRVIPDLAGIPRVIAAR

Sequences:

>Translated_272_residues
MNVQTALVQGTRLLEDAGVGVPRLTAEVLLGHAMRVQREYFYAHPEQELREVEWLHYGRYLHERLGGKPTQYITHRQEFY
SREFRVTPDVLIPRPETEHVVEAALEVARGAKRVLDVGTGSGALAITLRLEMGTEAWATDISAEAATVAAGNAARLGANV
HFAVCDLMDAIAADSMNLIVSNPPYVPLAHREGLQREVRDFEPHVALFGGQTGFELYDRIVADAPRVLRPGGWLIMELGF
TSLDHVRALLASWSDVRVIPDLAGIPRVIAAR
>Mature_272_residues
MNVQTALVQGTRLLEDAGVGVPRLTAEVLLGHAMRVQREYFYAHPEQELREVEWLHYGRYLHERLGGKPTQYITHRQEFY
SREFRVTPDVLIPRPETEHVVEAALEVARGAKRVLDVGTGSGALAITLRLEMGTEAWATDISAEAATVAAGNAARLGANV
HFAVCDLMDAIAADSMNLIVSNPPYVPLAHREGLQREVRDFEPHVALFGGQTGFELYDRIVADAPRVLRPGGWLIMELGF
TSLDHVRALLASWSDVRVIPDLAGIPRVIAAR

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=292, Percent_Identity=30.8219178082192, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI1787463, Length=256, Percent_Identity=34.765625, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082085, Length=203, Percent_Identity=33.0049261083744, Blast_Score=80, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24582226, Length=242, Percent_Identity=33.0578512396694, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30061; Mature: 30061

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVQTALVQGTRLLEDAGVGVPRLTAEVLLGHAMRVQREYFYAHPEQELREVEWLHYGRY
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LHERLGGKPTQYITHRQEFYSREFRVTPDVLIPRPETEHVVEAALEVARGAKRVLDVGTG
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHEECCCC
SGALAITLRLEMGTEAWATDISAEAATVAAGNAARLGANVHFAVCDLMDAIAADSMNLIV
CCEEEEEEEEECCCHHHHHCCCCCHHEEECCCHHHHCCCCHHHHHHHHHHHHCCCCEEEE
SNPPYVPLAHREGLQREVRDFEPHVALFGGQTGFELYDRIVADAPRVLRPGGWLIMELGF
CCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEEECC
TSLDHVRALLASWSDVRVIPDLAGIPRVIAAR
CCHHHHHHHHHCCCCCEECCCCCCCCHHHHCC
>Mature Secondary Structure
MNVQTALVQGTRLLEDAGVGVPRLTAEVLLGHAMRVQREYFYAHPEQELREVEWLHYGRY
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LHERLGGKPTQYITHRQEFYSREFRVTPDVLIPRPETEHVVEAALEVARGAKRVLDVGTG
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHEECCCC
SGALAITLRLEMGTEAWATDISAEAATVAAGNAARLGANVHFAVCDLMDAIAADSMNLIV
CCEEEEEEEEECCCHHHHHCCCCCHHEEECCCHHHHCCCCHHHHHHHHHHHHCCCCEEEE
SNPPYVPLAHREGLQREVRDFEPHVALFGGQTGFELYDRIVADAPRVLRPGGWLIMELGF
CCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEEECC
TSLDHVRALLASWSDVRVIPDLAGIPRVIAAR
CCHHHHHHHHHCCCCCEECCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]