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The map label for this gene is prfA

Identifier: 116625721

GI number: 116625721

Start: 8373899

End: 8374987

Strand: Reverse

Name: prfA

Synonym: Acid_6670

Alternate gene names: 116625721

Gene position: 8374987-8373899 (Counterclockwise)

Preceding gene: 116625722

Following gene: 116625720

Centisome position: 84.04

GC content: 60.7

Gene sequence:

>1089_bases
ATGCAATTCGCTCAGAAGCTCGATCAGCTCGAGAAGCGTTTCGACGAGCTAACCCAACAGATGGCCGATCCGGCCGTGAT
CAGCGATGCGGACCAGTACCGCAAAGTCGCAAAGGCCCAAAGTGAACTTTCCGATATCGCGGCGAAGTACCGCGAGTGGA
AGAAGGTAGAGGACGGCCTTGCCCAGGCCCGGCCGATGCTCCAGGAACAGGACGCCGAGCTGAAAGAAATGGCGGAACTG
GAGATCGCCCAGCTGGAGCCGGAGAAGCTGCGCATCGAAGAGGAACTGCGGGTTCTGCTGCTTCCCAAGGATCCGAACGA
CGATAAGAACGTGGTGCTGGAAATCCGCGCCGGCACGGGCGGCGACGAGGCTACGCTGTTCGCGGCGGAGATTTTCCGGA
TGTACTCGCGCTACGCGGAGACGCAGAACTGGAAGATGGAAGTGACGTCCAGCAGCGAATCGAGCGTCGGCGGGTTGAAA
GAAGTGATCGCGCTGGTGAGCGGTAATAAGGTTTACGCCAAGCTGAAGTACGAAAGCGGCGTACACCGCGTGCAGCGGGT
CCCGGTGACGGAGCAGCAGGGGCGGGTACACACGTCGGCGATTACGGTGGCGGTGCTGCCAGAGGCTGATGAGGTCGAGG
TCAAACTCGAGGCGAAGGACATTCGCATCGACACGTTCTGTTCGTCGGGTCCGGGCGGCCAGAGCGTCAATACGACGTAC
TCCGCTGTCCGCATCACGCATCTTCCGACTGGGCTGGTGGTTTCCTGCCAGGACGAAAAATCGCAGATCAAGAATCGGGC
GAAGGCGGAGCGCGTGCTGCGATCGCGGCTCTATGAGCTGGAGTTGGAGAAGCAGCAGGCAGCGCTGGGAGCCGAACGGC
GGACAATGGTGGGCTCGGGCGACCGTAGCGAAAAGATCCGCACGTACAATTTCCCGCAGAATCGCGTCAGCGATCACCGC
ATTGGGCTGACGCTGCATCAGCTGGATTTCGTGATGGAAGGAAAGATGGACGCGATCGTGGACGCGCTCACGGAGTTCTA
CCAGGCGCAGAAGCTCAAGCAGCAGGCGGAGAAGGGCCCGGTCAGCTAG

Upstream 100 bases:

>100_bases
CCTGCGGACGACCAGACTGCGGTCGCGATCCGCGCGCTGGAAGGCGCGATGGCTCTCGAAGAGCAGCAGGGCGGCGAGTT
GGTTATCGCATAAATTCTCC

Downstream 100 bases:

>100_bases
GGATCCTAACGGCACGTATTCCGGATAGTAGGGGCGGCCGCGTCGCAGCGGGCGAGCCGGCTGAAAGCCGGCTGCCGGCA
GGATTGCCTGCCCCACAGAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MQFAQKLDQLEKRFDELTQQMADPAVISDADQYRKVAKAQSELSDIAAKYREWKKVEDGLAQARPMLQEQDAELKEMAEL
EIAQLEPEKLRIEEELRVLLLPKDPNDDKNVVLEIRAGTGGDEATLFAAEIFRMYSRYAETQNWKMEVTSSSESSVGGLK
EVIALVSGNKVYAKLKYESGVHRVQRVPVTEQQGRVHTSAITVAVLPEADEVEVKLEAKDIRIDTFCSSGPGGQSVNTTY
SAVRITHLPTGLVVSCQDEKSQIKNRAKAERVLRSRLYELELEKQQAALGAERRTMVGSGDRSEKIRTYNFPQNRVSDHR
IGLTLHQLDFVMEGKMDAIVDALTEFYQAQKLKQQAEKGPVS

Sequences:

>Translated_362_residues
MQFAQKLDQLEKRFDELTQQMADPAVISDADQYRKVAKAQSELSDIAAKYREWKKVEDGLAQARPMLQEQDAELKEMAEL
EIAQLEPEKLRIEEELRVLLLPKDPNDDKNVVLEIRAGTGGDEATLFAAEIFRMYSRYAETQNWKMEVTSSSESSVGGLK
EVIALVSGNKVYAKLKYESGVHRVQRVPVTEQQGRVHTSAITVAVLPEADEVEVKLEAKDIRIDTFCSSGPGGQSVNTTY
SAVRITHLPTGLVVSCQDEKSQIKNRAKAERVLRSRLYELELEKQQAALGAERRTMVGSGDRSEKIRTYNFPQNRVSDHR
IGLTLHQLDFVMEGKMDAIVDALTEFYQAQKLKQQAEKGPVS
>Mature_362_residues
MQFAQKLDQLEKRFDELTQQMADPAVISDADQYRKVAKAQSELSDIAAKYREWKKVEDGLAQARPMLQEQDAELKEMAEL
EIAQLEPEKLRIEEELRVLLLPKDPNDDKNVVLEIRAGTGGDEATLFAAEIFRMYSRYAETQNWKMEVTSSSESSVGGLK
EVIALVSGNKVYAKLKYESGVHRVQRVPVTEQQGRVHTSAITVAVLPEADEVEVKLEAKDIRIDTFCSSGPGGQSVNTTY
SAVRITHLPTGLVVSCQDEKSQIKNRAKAERVLRSRLYELELEKQQAALGAERRTMVGSGDRSEKIRTYNFPQNRVSDHR
IGLTLHQLDFVMEGKMDAIVDALTEFYQAQKLKQQAEKGPVS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=295, Percent_Identity=46.4406779661017, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI34577120, Length=350, Percent_Identity=41.1428571428571, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI166795305, Length=185, Percent_Identity=47.027027027027, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1787462, Length=354, Percent_Identity=51.6949152542373, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=34.5132743362832, Blast_Score=187, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=278, Percent_Identity=39.2086330935252, Blast_Score=190, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=40.6060606060606, Blast_Score=232, Evalue=5e-62,
Organism=Drosophila melanogaster, GI19921226, Length=262, Percent_Identity=44.6564885496183, Blast_Score=231, Evalue=4e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SOLUE (Q01RX8)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827877.1
- ProteinModelPortal:   Q01RX8
- SMR:   Q01RX8
- STRING:   Q01RX8
- GeneID:   4425228
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_6670
- NMPDR:   fig|234267.9.peg.6341
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q01RX8
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40746; Mature: 40746

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFAQKLDQLEKRFDELTQQMADPAVISDADQYRKVAKAQSELSDIAAKYREWKKVEDGL
CCHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQARPMLQEQDAELKEMAELEIAQLEPEKLRIEEELRVLLLPKDPNDDKNVVLEIRAGTG
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCC
GDEATLFAAEIFRMYSRYAETQNWKMEVTSSSESSVGGLKEVIALVSGNKVYAKLKYESG
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEHHHH
VHRVQRVPVTEQQGRVHTSAITVAVLPEADEVEVKLEAKDIRIDTFCSSGPGGQSVNTTY
HHHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEEECEEEEEEEECCCCCCCCCCCEE
SAVRITHLPTGLVVSCQDEKSQIKNRAKAERVLRSRLYELELEKQQAALGAERRTMVGSG
EEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCC
DRSEKIRTYNFPQNRVSDHRIGLTLHQLDFVMEGKMDAIVDALTEFYQAQKLKQQAEKGP
CCCCCCEEECCCCHHCCCCHHCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
VS
CC
>Mature Secondary Structure
MQFAQKLDQLEKRFDELTQQMADPAVISDADQYRKVAKAQSELSDIAAKYREWKKVEDGL
CCHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQARPMLQEQDAELKEMAELEIAQLEPEKLRIEEELRVLLLPKDPNDDKNVVLEIRAGTG
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCC
GDEATLFAAEIFRMYSRYAETQNWKMEVTSSSESSVGGLKEVIALVSGNKVYAKLKYESG
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEHHHH
VHRVQRVPVTEQQGRVHTSAITVAVLPEADEVEVKLEAKDIRIDTFCSSGPGGQSVNTTY
HHHHHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEEEECEEEEEEEECCCCCCCCCCCEE
SAVRITHLPTGLVVSCQDEKSQIKNRAKAERVLRSRLYELELEKQQAALGAERRTMVGSG
EEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCC
DRSEKIRTYNFPQNRVSDHRIGLTLHQLDFVMEGKMDAIVDALTEFYQAQKLKQQAEKGP
CCCCCCEEECCCCHHCCCCHHCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
VS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA