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The map label for this gene is nifA [H]

Identifier: 116625691

GI number: 116625691

Start: 8327919

End: 8329448

Strand: Direct

Name: nifA [H]

Synonym: Acid_6640

Alternate gene names: 116625691

Gene position: 8327919-8329448 (Clockwise)

Preceding gene: 116625690

Following gene: 116625695

Centisome position: 83.57

GC content: 62.55

Gene sequence:

>1530_bases
ATGAGCTCGCTTCCCCAACCGGAATTGAATGAACGGCTGGCGGCCATTCTTGCCATCTCGCAAAAGATGAACTCGCAGCG
CGATCTGAGCGAGCTCCTCGACCTGATCGCGCGCGAAGCCACCAGCCTGTTGGATTGCGACCGCGCGAGCATTTTCCTGC
TGGATCGCGACACCAACGAGTTGTGGTCGAAGGTCGCGCTTGGGTCCGACGAAATCCTGCGCTTCGAGGCGAGCCGAGGG
ATCGCCGGCAACGCTGCGCGTACGGGCGACACGATCAACGTGCGCGATGCATACAGCGATCCGAGGTTCTACACCGGCAT
CGACGGCAAGACCGGCTATCGCACGCGGAACCTGCTCGCGGTGGCAGTTCGAAACCAGCGCGGGGAAATCATCGGCGCCT
TCGAGGCATTAAACAAGCGCGTGGGAGTTTTCACTGCGCGCGATGAGGAATCGCTGGCGGCGCTGGCGTCTCACGCGGCG
GTGGCCATCGAAACCGCGCAGCTGATCGGGGAATTGCGACGCAATCAGGACGAGTTGGCCCAGCAGAACGCGCATTTGTG
GAGGGAGGTGGAGAGCCGCTACGCCACGCACGGGATCGTCGGCTCCGGCCAGCGCATCCAGCAGGTGGTGCGGCTGGTGG
AGCGGATCCGCGACAGCGTGGTCAACGTGCTGATTACCGGGGAGACGGGGACCGGCAAAGAGCTCGCGGCCAAGGCGATC
CACTACAACAGTCCGAGGGTGCGCAAGCCATTCGTCGCGCTCAACTGCGCGGCGCTACCGGAAACGCTCCTCGAAAGCGA
GCTCTTCGGCATCGAAAAGGGGGTGGCGACGGGCGTCAATTCGCGAGTCGGCCAGTTTCAGAAGGCCGACGGCGGGACAC
TATTCCTGGACGAGATCGGCGACTTGAGCCTGACCGCGCAAGCGAAAATCCTACGCGTGTTGCAGGAACGCGTGCTGGAG
CGTGTGGGCGGACGCGCTCCGATCGCGGTGGACGTGCGGCTGCTAGCGGCGACAAATAAGGATCTGGAGGCCGAAATCGC
CAAGGGCAATTTCCGCGAGGATCTTTACTACCGGATCAAGGTCATACACATCCACATGCCTCCGCTGCGCGAGATCCGCG
AGGAAATCCCGCTGCTGGCGCGCCATTTCCTGAAGGAATACTGCCGCGAAACCGGCCGCGAGGCCATGGAGTTTGCGCCC
GACGTCATGCGCAAACTGGTGAATTCACCGTGGCCGGGGAACGTCAGGCAATTGCGCAACGAGGTCACACGGCTGGCGGC
ATGCGCACGTCTGTCGTTGATCGAGGAAGAGGATCTCTGGGAAGGTATCCCGCTGGTTGCCCGCCATGTGCCGACGGCAG
AGCCGGTCAAACTACAGTCTCTCAAGAAGGCGGTGGCTGAGATGGAGGGTCGAATGATCGCCGAGGCGCTGCAGGAGACC
CGGAATAATCAGCAGCAGGCCGCCAAACTGCTGGGTCTCAGCCGCCAAGGGCTCATCAACAAGATGAAGCGGTACGCGAT
CGCGGGCTAA

Upstream 100 bases:

>100_bases
CCACCCTGTCCAGCCGGTTTACGGACCGTCCACATCGTTGGCACCTGTCCACTTCGTGGACACTACCACACAGACTGGAG
TAGACTGAAAGCCTTCCCCC

Downstream 100 bases:

>100_bases
AACCGGCAGCGGCCGATACAGCCCTTTCCGACACACGTTTTGCAGCAGGGGGTCTTGCGTAGCTTTCCGGTCAAGAGTGC
TCGTGCCGGTGCTGTGGCAG

Product: NifA subfamily transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSSLPQPELNERLAAILAISQKMNSQRDLSELLDLIAREATSLLDCDRASIFLLDRDTNELWSKVALGSDEILRFEASRG
IAGNAARTGDTINVRDAYSDPRFYTGIDGKTGYRTRNLLAVAVRNQRGEIIGAFEALNKRVGVFTARDEESLAALASHAA
VAIETAQLIGELRRNQDELAQQNAHLWREVESRYATHGIVGSGQRIQQVVRLVERIRDSVVNVLITGETGTGKELAAKAI
HYNSPRVRKPFVALNCAALPETLLESELFGIEKGVATGVNSRVGQFQKADGGTLFLDEIGDLSLTAQAKILRVLQERVLE
RVGGRAPIAVDVRLLAATNKDLEAEIAKGNFREDLYYRIKVIHIHMPPLREIREEIPLLARHFLKEYCRETGREAMEFAP
DVMRKLVNSPWPGNVRQLRNEVTRLAACARLSLIEEEDLWEGIPLVARHVPTAEPVKLQSLKKAVAEMEGRMIAEALQET
RNNQQQAAKLLGLSRQGLINKMKRYAIAG

Sequences:

>Translated_509_residues
MSSLPQPELNERLAAILAISQKMNSQRDLSELLDLIAREATSLLDCDRASIFLLDRDTNELWSKVALGSDEILRFEASRG
IAGNAARTGDTINVRDAYSDPRFYTGIDGKTGYRTRNLLAVAVRNQRGEIIGAFEALNKRVGVFTARDEESLAALASHAA
VAIETAQLIGELRRNQDELAQQNAHLWREVESRYATHGIVGSGQRIQQVVRLVERIRDSVVNVLITGETGTGKELAAKAI
HYNSPRVRKPFVALNCAALPETLLESELFGIEKGVATGVNSRVGQFQKADGGTLFLDEIGDLSLTAQAKILRVLQERVLE
RVGGRAPIAVDVRLLAATNKDLEAEIAKGNFREDLYYRIKVIHIHMPPLREIREEIPLLARHFLKEYCRETGREAMEFAP
DVMRKLVNSPWPGNVRQLRNEVTRLAACARLSLIEEEDLWEGIPLVARHVPTAEPVKLQSLKKAVAEMEGRMIAEALQET
RNNQQQAAKLLGLSRQGLINKMKRYAIAG
>Mature_508_residues
SSLPQPELNERLAAILAISQKMNSQRDLSELLDLIAREATSLLDCDRASIFLLDRDTNELWSKVALGSDEILRFEASRGI
AGNAARTGDTINVRDAYSDPRFYTGIDGKTGYRTRNLLAVAVRNQRGEIIGAFEALNKRVGVFTARDEESLAALASHAAV
AIETAQLIGELRRNQDELAQQNAHLWREVESRYATHGIVGSGQRIQQVVRLVERIRDSVVNVLITGETGTGKELAAKAIH
YNSPRVRKPFVALNCAALPETLLESELFGIEKGVATGVNSRVGQFQKADGGTLFLDEIGDLSLTAQAKILRVLQERVLER
VGGRAPIAVDVRLLAATNKDLEAEIAKGNFREDLYYRIKVIHIHMPPLREIREEIPLLARHFLKEYCRETGREAMEFAPD
VMRKLVNSPWPGNVRQLRNEVTRLAACARLSLIEEEDLWEGIPLVARHVPTAEPVKLQSLKKAVAEMEGRMIAEALQETR
NNQQQAAKLLGLSRQGLINKMKRYAIAG

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Homo sapiens, GI4505657, Length=183, Percent_Identity=36.6120218579235, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI219802706, Length=183, Percent_Identity=36.6120218579235, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI61744432, Length=185, Percent_Identity=32.4324324324324, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI61744430, Length=185, Percent_Identity=32.4324324324324, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI226246591, Length=183, Percent_Identity=36.6120218579235, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI61744435, Length=185, Percent_Identity=32.4324324324324, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI5729972, Length=229, Percent_Identity=31.0043668122271, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI194595488, Length=229, Percent_Identity=31.0043668122271, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI116536085, Length=184, Percent_Identity=30.9782608695652, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=327, Percent_Identity=45.565749235474, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1790299, Length=342, Percent_Identity=44.4444444444444, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI1790437, Length=308, Percent_Identity=45.4545454545455, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1789087, Length=505, Percent_Identity=34.2574257425743, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI87082117, Length=522, Percent_Identity=33.7164750957854, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=42.2360248447205, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788905, Length=306, Percent_Identity=42.483660130719, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI87082152, Length=301, Percent_Identity=43.8538205980066, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=39.0625, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1786524, Length=356, Percent_Identity=36.7977528089888, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1787583, Length=311, Percent_Identity=36.6559485530547, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081858, Length=315, Percent_Identity=31.1111111111111, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789828, Length=343, Percent_Identity=30.6122448979592, Blast_Score=125, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI193202333, Length=189, Percent_Identity=32.8042328042328, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161076953, Length=183, Percent_Identity=28.9617486338798, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161076955, Length=183, Percent_Identity=28.9617486338798, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281361762, Length=103, Percent_Identity=39.8058252427184, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56599; Mature: 56467

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLPQPELNERLAAILAISQKMNSQRDLSELLDLIAREATSLLDCDRASIFLLDRDTNE
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHH
LWSKVALGSDEILRFEASRGIAGNAARTGDTINVRDAYSDPRFYTGIDGKTGYRTRNLLA
HHHHHHCCCCHHEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCCCHHCEEE
VAVRNQRGEIIGAFEALNKRVGVFTARDEESLAALASHAAVAIETAQLIGELRRNQDELA
EEEECCCCCEEEHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
QQNAHLWREVESRYATHGIVGSGQRIQQVVRLVERIRDSVVNVLITGETGTGKELAAKAI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHH
HYNSPRVRKPFVALNCAALPETLLESELFGIEKGVATGVNSRVGQFQKADGGTLFLDEIG
HCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCC
DLSLTAQAKILRVLQERVLERVGGRAPIAVDVRLLAATNKDLEAEIAKGNFREDLYYRIK
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHCCCCHHCCEEEEE
VIHIHMPPLREIREEIPLLARHFLKEYCRETGREAMEFAPDVMRKLVNSPWPGNVRQLRN
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
EVTRLAACARLSLIEEEDLWEGIPLVARHVPTAEPVKLQSLKKAVAEMEGRMIAEALQET
HHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RNNQQQAAKLLGLSRQGLINKMKRYAIAG
HCCHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSLPQPELNERLAAILAISQKMNSQRDLSELLDLIAREATSLLDCDRASIFLLDRDTNE
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHH
LWSKVALGSDEILRFEASRGIAGNAARTGDTINVRDAYSDPRFYTGIDGKTGYRTRNLLA
HHHHHHCCCCHHEEEECCCCCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCCCHHCEEE
VAVRNQRGEIIGAFEALNKRVGVFTARDEESLAALASHAAVAIETAQLIGELRRNQDELA
EEEECCCCCEEEHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
QQNAHLWREVESRYATHGIVGSGQRIQQVVRLVERIRDSVVNVLITGETGTGKELAAKAI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHH
HYNSPRVRKPFVALNCAALPETLLESELFGIEKGVATGVNSRVGQFQKADGGTLFLDEIG
HCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEECCC
DLSLTAQAKILRVLQERVLERVGGRAPIAVDVRLLAATNKDLEAEIAKGNFREDLYYRIK
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHCCCCHHCCEEEEE
VIHIHMPPLREIREEIPLLARHFLKEYCRETGREAMEFAPDVMRKLVNSPWPGNVRQLRN
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
EVTRLAACARLSLIEEEDLWEGIPLVARHVPTAEPVKLQSLKKAVAEMEGRMIAEALQET
HHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RNNQQQAAKLLGLSRQGLINKMKRYAIAG
HCCHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]