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The map label for this gene is rihA [H]

Identifier: 116625519

GI number: 116625519

Start: 8104859

End: 8105797

Strand: Direct

Name: rihA [H]

Synonym: Acid_6466

Alternate gene names: 116625519

Gene position: 8104859-8105797 (Clockwise)

Preceding gene: 116625513

Following gene: 116625520

Centisome position: 81.33

GC content: 64.96

Gene sequence:

>939_bases
TTGATCGACACAGATACAGCGTCTGACGATGCGGTCGCGCTGATCATGGCGCTGCGCAATCCGAGTGTTTTCGTACGTGC
CATCACGACCGTCGCAGGAAATGTACCGGTTGCGCAGGCAACGCGCAACGCGCTCTACGTCGCGCAACTTTGCGGCTCCG
ACGTGCCCGTGTACCAGGGTGCCGCACGGCCGCTGTGGCGGGAGCACGCGCATGCCGAGTGGTTTCATGGCAAGGACGGC
CTGGGCGATCATGGCTACCCGGCTCCGGCGCGAGCGGCGGAGCCCGGCAATGCGGCGGACGCGATCATCGCCGCGATCGA
ACAGCATCCCGGCATCGTGGTGGTGACGCTCGGGCCGCTTACAAACATGGCCGTGGCACTCGCCAAGGCTCCGGACATCG
CGGCAAAGGTCGGCCGCTGCGTGGTGATGGGAGGTAATCCCTGCTGTGAGGGCAATGTAACACCGGCGGCGGAGTACAAC
ATGTGGGTTGACCCCGAGGCGGCGCGAATCGTCCTGCGCTCTGGCCTGCCGGTGGAACTGGCGGGCTGGCATCTCTGCCG
CGGCGAAGCGGTGCTCGGCGCGGCGGATATCGAGCACGTGCTTTCGCTCAACACGCCGCTCGCCAGGTTCGCCGTGGAAT
GCAACTCGCGCGCGATGGATGCCTACCGCATTCAGACCGGCGAGTTGGGAATCTCGCTGCCGGACCCGGTGGCGATGTCG
ATCGCGCTCGACCCCGTGGTGTGCACGCAACTAAGTTCGCACTATGTGGATGTCGAAGTGAGCAGTGAGTTGACGCGCGG
GATGACTGTGGTGGACCGGCTCAACGTGTCGGGCGACGAACGCAATTCGCCGGTATGGCGCGGCACGCTACAGGCGCAGG
TAGTGTGGACCATCGACAATGAGCGCTGGAAGCAAGCGTTGTACGCGGCGCTCGGTTAG

Upstream 100 bases:

>100_bases
TATGAACGCGAATCCGCGAGCTTGTCAAGAAGTTTACGTGAATTCGTCACAGAATCTTCAAGATTCGCAGTGGTACGCTG
ATACCTTGTCCCGGGACCTT

Downstream 100 bases:

>100_bases
TGCGGCTCGCTGGCGAGTTCGTCGTCCTTGCTAAACTCGGAAGTTGAACCCTACAGCTCAGATCATCGTCCTCGATGCGG
GGGGGCAGTATTGCCACCTC

Product: inosine/uridine-preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIDTDTASDDAVALIMALRNPSVFVRAITTVAGNVPVAQATRNALYVAQLCGSDVPVYQGAARPLWREHAHAEWFHGKDG
LGDHGYPAPARAAEPGNAADAIIAAIEQHPGIVVVTLGPLTNMAVALAKAPDIAAKVGRCVVMGGNPCCEGNVTPAAEYN
MWVDPEAARIVLRSGLPVELAGWHLCRGEAVLGAADIEHVLSLNTPLARFAVECNSRAMDAYRIQTGELGISLPDPVAMS
IALDPVVCTQLSSHYVDVEVSSELTRGMTVVDRLNVSGDERNSPVWRGTLQAQVVWTIDNERWKQALYAALG

Sequences:

>Translated_312_residues
MIDTDTASDDAVALIMALRNPSVFVRAITTVAGNVPVAQATRNALYVAQLCGSDVPVYQGAARPLWREHAHAEWFHGKDG
LGDHGYPAPARAAEPGNAADAIIAAIEQHPGIVVVTLGPLTNMAVALAKAPDIAAKVGRCVVMGGNPCCEGNVTPAAEYN
MWVDPEAARIVLRSGLPVELAGWHLCRGEAVLGAADIEHVLSLNTPLARFAVECNSRAMDAYRIQTGELGISLPDPVAMS
IALDPVVCTQLSSHYVDVEVSSELTRGMTVVDRLNVSGDERNSPVWRGTLQAQVVWTIDNERWKQALYAALG
>Mature_312_residues
MIDTDTASDDAVALIMALRNPSVFVRAITTVAGNVPVAQATRNALYVAQLCGSDVPVYQGAARPLWREHAHAEWFHGKDG
LGDHGYPAPARAAEPGNAADAIIAAIEQHPGIVVVTLGPLTNMAVALAKAPDIAAKVGRCVVMGGNPCCEGNVTPAAEYN
MWVDPEAARIVLRSGLPVELAGWHLCRGEAVLGAADIEHVLSLNTPLARFAVECNSRAMDAYRIQTGELGISLPDPVAMS
IALDPVVCTQLSSHYVDVEVSSELTRGMTVVDRLNVSGDERNSPVWRGTLQAQVVWTIDNERWKQALYAALG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=288, Percent_Identity=35.0694444444444, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1788486, Length=286, Percent_Identity=32.8671328671329, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786213, Length=306, Percent_Identity=32.6797385620915, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32565474, Length=293, Percent_Identity=35.4948805460751, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17565698, Length=297, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320608, Length=254, Percent_Identity=29.9212598425197, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24641839, Length=332, Percent_Identity=31.9277108433735, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24583915, Length=295, Percent_Identity=31.864406779661, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24641837, Length=199, Percent_Identity=32.1608040201005, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33159; Mature: 33159

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTDTASDDAVALIMALRNPSVFVRAITTVAGNVPVAQATRNALYVAQLCGSDVPVYQG
CCCCCCCCCCCEEEEEECCCCCEEEEEHHHHCCCCCHHHHHHHHEEEEHHHCCCCCCCCC
AARPLWREHAHAEWFHGKDGLGDHGYPAPARAAEPGNAADAIIAAIEQHPGIVVVTLGPL
CCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCH
TNMAVALAKAPDIAAKVGRCVVMGGNPCCEGNVTPAAEYNMWVDPEAARIVLRSGLPVEL
HHHHHHHHCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCEEECCHHHHEEECCCCCEEE
AGWHLCRGEAVLGAADIEHVLSLNTPLARFAVECNSRAMDAYRIQTGELGISLPDPVAMS
CCEEEECCCEEECHHHHHHHHHCCCCHHHHEECCCCCCCEEEEEEECCCCCCCCCCEEEE
IALDPVVCTQLSSHYVDVEVSSELTRGMTVVDRLNVSGDERNSPVWRGTLQAQVVWTIDN
EEECHHHHHHHHCEEEEEEECCHHHCCCEEEEEECCCCCCCCCCEEEEEEEEEEEEEECC
ERWKQALYAALG
HHHHHHHHHHCC
>Mature Secondary Structure
MIDTDTASDDAVALIMALRNPSVFVRAITTVAGNVPVAQATRNALYVAQLCGSDVPVYQG
CCCCCCCCCCCEEEEEECCCCCEEEEEHHHHCCCCCHHHHHHHHEEEEHHHCCCCCCCCC
AARPLWREHAHAEWFHGKDGLGDHGYPAPARAAEPGNAADAIIAAIEQHPGIVVVTLGPL
CCCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCH
TNMAVALAKAPDIAAKVGRCVVMGGNPCCEGNVTPAAEYNMWVDPEAARIVLRSGLPVEL
HHHHHHHHCCCCHHHHCCEEEEECCCCCCCCCCCCCCCCCEEECCHHHHEEECCCCCEEE
AGWHLCRGEAVLGAADIEHVLSLNTPLARFAVECNSRAMDAYRIQTGELGISLPDPVAMS
CCEEEECCCEEECHHHHHHHHHCCCCHHHHEECCCCCCCEEEEEEECCCCCCCCCCEEEE
IALDPVVCTQLSSHYVDVEVSSELTRGMTVVDRLNVSGDERNSPVWRGTLQAQVVWTIDN
EEECHHHHHHHHCEEEEEEECCHHHCCCEEEEEECCCCCCCCCCEEEEEEEEEEEEEECC
ERWKQALYAALG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA