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The map label for this gene is ruvC

Identifier: 116625019

GI number: 116625019

Start: 7459211

End: 7459711

Strand: Direct

Name: ruvC

Synonym: Acid_5949

Alternate gene names: 116625019

Gene position: 7459211-7459711 (Clockwise)

Preceding gene: 116625018

Following gene: 116625020

Centisome position: 74.85

GC content: 62.28

Gene sequence:

>501_bases
GTGCGCGTCCTGGGCGTCGATTGCGGTTCAGAACGTACTGGCTGGGGCGTGATCGATAGCGACGGAGGGTGCCACCGGAT
GGTTGCCGCGGGGGTGATTCGGACCAGTCCGAAATGCCCGTTCGACCGGCGGCTGCTGGAGATTGGAACGGCGCTGCGGG
GCTTGATTCGGGAGCACACACCCGAATTTGCCGCGGTGGAAGAGGTTTTCCACGCGGTCAACGCCAAAACGGCATTGAAG
CTCGCGCATGTGCGGGGGGTCGCCCTGCTGACGATCGCGGAAGCAGGGGTCGTGTTCGCGGAGTACTCTCCGCTGGAAGT
TAAAATGAGCGTAGTGGGGTACGGCAGGGCGGAGAAAAGCCAGGTGCAGATGATGGTGCAATCTCTGTTGCGATTACCGG
AAGTGATCGCGAGCGAAGATGCCTCCGATGCCCTGGCAATCGCGATCTGCCACGCAACCCACCAGGCCACCCACGAAGCA
ACCAGCCGGAGATTGGGCTAG

Upstream 100 bases:

>100_bases
CTCCGGCTCACCGAAGTTCTGGAAGACCACGACGATGTGCAGAGCGTCTATTCCAACTTCGACATCGATGAGAAGGAGTT
GGAGGCGCTGTCGCAGTAAG

Downstream 100 bases:

>100_bases
CAGGTACCTATGAAGTGGCTTCTTATTCTGTGGTTCGCGGGCACGCTGGTCGCACAGGACGCCCAGCGGTTCTTCTACTC
AAAGGCCTTTCCCAATAGCG

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MRVLGVDCGSERTGWGVIDSDGGCHRMVAAGVIRTSPKCPFDRRLLEIGTALRGLIREHTPEFAAVEEVFHAVNAKTALK
LAHVRGVALLTIAEAGVVFAEYSPLEVKMSVVGYGRAEKSQVQMMVQSLLRLPEVIASEDASDALAIAICHATHQATHEA
TSRRLG

Sequences:

>Translated_166_residues
MRVLGVDCGSERTGWGVIDSDGGCHRMVAAGVIRTSPKCPFDRRLLEIGTALRGLIREHTPEFAAVEEVFHAVNAKTALK
LAHVRGVALLTIAEAGVVFAEYSPLEVKMSVVGYGRAEKSQVQMMVQSLLRLPEVIASEDASDALAIAICHATHQATHEA
TSRRLG
>Mature_166_residues
MRVLGVDCGSERTGWGVIDSDGGCHRMVAAGVIRTSPKCPFDRRLLEIGTALRGLIREHTPEFAAVEEVFHAVNAKTALK
LAHVRGVALLTIAEAGVVFAEYSPLEVKMSVVGYGRAEKSQVQMMVQSLLRLPEVIASEDASDALAIAICHATHQATHEA
TSRRLG

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=162, Percent_Identity=38.2716049382716, Blast_Score=97, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_SOLUE (Q01TY0)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827175.1
- ProteinModelPortal:   Q01TY0
- SMR:   Q01TY0
- STRING:   Q01TY0
- GeneID:   4425834
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_5949
- NMPDR:   fig|234267.9.peg.5660
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q01TY0
- ProtClustDB:   CLSK775008
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17831; Mature: 17831

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLGVDCGSERTGWGVIDSDGGCHRMVAAGVIRTSPKCPFDRRLLEIGTALRGLIREHT
CEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
PEFAAVEEVFHAVNAKTALKLAHVRGVALLTIAEAGVVFAEYSPLEVKMSVVGYGRAEKS
CCHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEHHCCEEEEECCCCEEEEEEEECCCCHHH
QVQMMVQSLLRLPEVIASEDASDALAIAICHATHQATHEATSRRLG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVLGVDCGSERTGWGVIDSDGGCHRMVAAGVIRTSPKCPFDRRLLEIGTALRGLIREHT
CEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
PEFAAVEEVFHAVNAKTALKLAHVRGVALLTIAEAGVVFAEYSPLEVKMSVVGYGRAEKS
CCHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEHHCCEEEEECCCCEEEEEEEECCCCHHH
QVQMMVQSLLRLPEVIASEDASDALAIAICHATHQATHEATSRRLG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA