Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 116624780

GI number: 116624780

Start: 7146563

End: 7148092

Strand: Direct

Name: leuA [H]

Synonym: Acid_5704

Alternate gene names: 116624780

Gene position: 7146563-7148092 (Clockwise)

Preceding gene: 116624779

Following gene: 116624781

Centisome position: 71.71

GC content: 65.1

Gene sequence:

>1530_bases
TTGAGGGACGGGGAACAAGCCGCCGGAACCCGCCTGGGAAGCCGCGAGAAGCTCATCATCGCCCGCCAACTCGCCCTGCT
CAACGTTGACGTCATTGAGGCCGGCTATCCCAACTCCTCGCCGGAGGATTTCGACGCCGTCCAGCGCATCGCCGCCGAGA
TTCAGGGGCCCGCCATCTGCGCGCTCTCCCGCGCTGTGGCCGCCGATATCGAGGCCTGCGGTAAAGCCCTCGCCGGCTGC
CCCCGCCCTCGCATTCATACCGGCATCGGCGCCTCCGACATCCACATCGCCGGCAAATTTCAGGACGATCGCTACGGCAA
ATCGCTCGCCGAAAAGAAGCGCAAGATCCTCCAGATGGCGATCGATGCCGTCACGCTCGCCCGCCACTATGTCGCTGAGG
TCGAGTTCTACGCCGAGGATGCCGGCCGCGCCGATCCTGTCTACCTTTATGAGATGATCGCCGCCGTCATCGACGCCGGC
GCCTCCGTCGTCAACATCCCCGACACCACTGGCTACACCGTGCCGGAACAGTACGGCGCCCTGATCGCCGGCATCCGCAC
CCATGTGCCCGGCATCGATCGAGCCGTCATCAGCGTCCACTGTCACGACGATCTCGGCATGGCGGTAGCCAATACCCTGC
ATGGCGTGCTCAACGGCGCCCGCCAGGTCGAAGGCACCATCAACGGGCTCGGCGAGCGCGCAGGTAATGCCGCGCTAGAG
GAAATCGTGATGGCCCTCGCCACGCGCGCCGACTATTTCGGCGTCCACACCGGCGTCAACACCCGCGAACTCCATCGCAC
CAGCCGCCTGGTCGCCGACCTGCTCGGCGTACAGGTCCCACCCAACAAGGCCGTCATCGGCGCGAACGCCTTCTCCCACA
GCTCCGGCATTCACGTCGATGGCGTTCTCAAAAAACGCGAGACCTACGAGATCATGCGGCCCGAGGATGTCGGCATGACC
GAGAGCCGTGTCGTCCTCACTGCCCGCACCGGCCGCCACGGCCTCCTCGACCGGCTCCGCAAGCTCGGCTATGAACTCTC
CGATCTCGAGCTGGATCAGGCCTATCGCCGCTTCCTCAGCGTCGCGGACAAAAAGCAGGAGGTCTTCGACGAAGATCTCA
TCGCCATCCTGCGCGACGAAATCCACCCGCTGCCCGCGGTTTATGAGCTCCAGTACCTGCACATCTACAGCGGTACTTCG
GCCATTCCCACGGCCACCGTAAGATTGCTGGTTAGCGGCGACACGCGCGAAGGCGCCGCGATCGGCGATGGTCCCGTGGA
CGCGGTCTGCCGGGCGATCTCACAGGTCACTGCCACCGCCGCCAAACTGCAACGCTACGAAATCCGCGGCGTCACCAGCG
GCACCGAGGCCATGGGCGAAGTCACCATCCAACTCGAGGAGGCCGGCCGCAAGGTGATCGGCCGCGGCGCTTCCACCGAT
ATCATCGAAGCCAGCGCCAAGGCCTACCTCGACGGTCTCAACAAGCTCGCCAACATCGCTGCCGCTTCCCACCGTTCCGC
CGGCGCATAG

Upstream 100 bases:

>100_bases
AAAGTTCGAAGAATTCTCCACTCTGCTGGGGTCAGCGAAAGTAAAATCAGCCATGCCGCCAAAAACAGTCTCTGAAAAAG
TAGCCGTCTTCGATACCACG

Downstream 100 bases:

>100_bases
CCTGCCAACGAGCCGCGACCGCAAGGGAGCGATTCCTAGTACGTGTAAACCACGTTTCGGTTTGCGGCTCTTGCCGCCTT
GTGGGGCAGGCAATCCTGCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MRDGEQAAGTRLGSREKLIIARQLALLNVDVIEAGYPNSSPEDFDAVQRIAAEIQGPAICALSRAVAADIEACGKALAGC
PRPRIHTGIGASDIHIAGKFQDDRYGKSLAEKKRKILQMAIDAVTLARHYVAEVEFYAEDAGRADPVYLYEMIAAVIDAG
ASVVNIPDTTGYTVPEQYGALIAGIRTHVPGIDRAVISVHCHDDLGMAVANTLHGVLNGARQVEGTINGLGERAGNAALE
EIVMALATRADYFGVHTGVNTRELHRTSRLVADLLGVQVPPNKAVIGANAFSHSSGIHVDGVLKKRETYEIMRPEDVGMT
ESRVVLTARTGRHGLLDRLRKLGYELSDLELDQAYRRFLSVADKKQEVFDEDLIAILRDEIHPLPAVYELQYLHIYSGTS
AIPTATVRLLVSGDTREGAAIGDGPVDAVCRAISQVTATAAKLQRYEIRGVTSGTEAMGEVTIQLEEAGRKVIGRGASTD
IIEASAKAYLDGLNKLANIAAASHRSAGA

Sequences:

>Translated_509_residues
MRDGEQAAGTRLGSREKLIIARQLALLNVDVIEAGYPNSSPEDFDAVQRIAAEIQGPAICALSRAVAADIEACGKALAGC
PRPRIHTGIGASDIHIAGKFQDDRYGKSLAEKKRKILQMAIDAVTLARHYVAEVEFYAEDAGRADPVYLYEMIAAVIDAG
ASVVNIPDTTGYTVPEQYGALIAGIRTHVPGIDRAVISVHCHDDLGMAVANTLHGVLNGARQVEGTINGLGERAGNAALE
EIVMALATRADYFGVHTGVNTRELHRTSRLVADLLGVQVPPNKAVIGANAFSHSSGIHVDGVLKKRETYEIMRPEDVGMT
ESRVVLTARTGRHGLLDRLRKLGYELSDLELDQAYRRFLSVADKKQEVFDEDLIAILRDEIHPLPAVYELQYLHIYSGTS
AIPTATVRLLVSGDTREGAAIGDGPVDAVCRAISQVTATAAKLQRYEIRGVTSGTEAMGEVTIQLEEAGRKVIGRGASTD
IIEASAKAYLDGLNKLANIAAASHRSAGA
>Mature_509_residues
MRDGEQAAGTRLGSREKLIIARQLALLNVDVIEAGYPNSSPEDFDAVQRIAAEIQGPAICALSRAVAADIEACGKALAGC
PRPRIHTGIGASDIHIAGKFQDDRYGKSLAEKKRKILQMAIDAVTLARHYVAEVEFYAEDAGRADPVYLYEMIAAVIDAG
ASVVNIPDTTGYTVPEQYGALIAGIRTHVPGIDRAVISVHCHDDLGMAVANTLHGVLNGARQVEGTINGLGERAGNAALE
EIVMALATRADYFGVHTGVNTRELHRTSRLVADLLGVQVPPNKAVIGANAFSHSSGIHVDGVLKKRETYEIMRPEDVGMT
ESRVVLTARTGRHGLLDRLRKLGYELSDLELDQAYRRFLSVADKKQEVFDEDLIAILRDEIHPLPAVYELQYLHIYSGTS
AIPTATVRLLVSGDTREGAAIGDGPVDAVCRAISQVTATAAKLQRYEIRGVTSGTEAMGEVTIQLEEAGRKVIGRGASTD
IIEASAKAYLDGLNKLANIAAASHRSAGA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=497, Percent_Identity=45.0704225352113, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320071, Length=382, Percent_Identity=28.7958115183246, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320019, Length=382, Percent_Identity=28.2722513089005, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324682, Length=554, Percent_Identity=25.0902527075812, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324225, Length=549, Percent_Identity=24.408014571949, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 54440; Mature: 54440

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDGEQAAGTRLGSREKLIIARQLALLNVDVIEAGYPNSSPEDFDAVQRIAAEIQGPAIC
CCCCCHHHHCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHH
ALSRAVAADIEACGKALAGCPRPRIHTGIGASDIHIAGKFQDDRYGKSLAEKKRKILQMA
HHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
IDAVTLARHYVAEVEFYAEDAGRADPVYLYEMIAAVIDAGASVVNIPDTTGYTVPEQYGA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHH
LIAGIRTHVPGIDRAVISVHCHDDLGMAVANTLHGVLNGARQVEGTINGLGERAGNAALE
HHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EIVMALATRADYFGVHTGVNTRELHRTSRLVADLLGVQVPPNKAVIGANAFSHSSGIHVD
HHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEH
GVLKKRETYEIMRPEDVGMTESRVVLTARTGRHGLLDRLRKLGYELSDLELDQAYRRFLS
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VADKKQEVFDEDLIAILRDEIHPLPAVYELQYLHIYSGTSAIPTATVRLLVSGDTREGAA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCC
IGDGPVDAVCRAISQVTATAAKLQRYEIRGVTSGTEAMGEVTIQLEEAGRKVIGRGASTD
CCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHEEEEEHHHCHHHHCCCCCCH
IIEASAKAYLDGLNKLANIAAASHRSAGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRDGEQAAGTRLGSREKLIIARQLALLNVDVIEAGYPNSSPEDFDAVQRIAAEIQGPAIC
CCCCCHHHHCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHH
ALSRAVAADIEACGKALAGCPRPRIHTGIGASDIHIAGKFQDDRYGKSLAEKKRKILQMA
HHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
IDAVTLARHYVAEVEFYAEDAGRADPVYLYEMIAAVIDAGASVVNIPDTTGYTVPEQYGA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHH
LIAGIRTHVPGIDRAVISVHCHDDLGMAVANTLHGVLNGARQVEGTINGLGERAGNAALE
HHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EIVMALATRADYFGVHTGVNTRELHRTSRLVADLLGVQVPPNKAVIGANAFSHSSGIHVD
HHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEH
GVLKKRETYEIMRPEDVGMTESRVVLTARTGRHGLLDRLRKLGYELSDLELDQAYRRFLS
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VADKKQEVFDEDLIAILRDEIHPLPAVYELQYLHIYSGTSAIPTATVRLLVSGDTREGAA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCC
IGDGPVDAVCRAISQVTATAAKLQRYEIRGVTSGTEAMGEVTIQLEEAGRKVIGRGASTD
CCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHEEEEEHHHCHHHHCCCCCCH
IIEASAKAYLDGLNKLANIAAASHRSAGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA