Click here to switch to the map view.

The map label for this gene is nuoN2

Identifier: 116624766

GI number: 116624766

Start: 7127363

End: 7128844

Strand: Direct

Name: nuoN2

Synonym: Acid_5690

Alternate gene names: 116624766

Gene position: 7127363-7128844 (Clockwise)

Preceding gene: 116624765

Following gene: 116624767

Centisome position: 71.52

GC content: 61.4

Gene sequence:

>1482_bases
ATGATCGATTTCAGCCTGTTCTACAATTCCACCGATCACTTCTCGATCATTCCGGCGGTCATGCTGGCGCTCTTCGGCTG
CGCCATCCTGCTCTTCGATTTCCTGATCTTCCCGGATCCGCGCCAGCGCAAGTTCCTGCTGATCTTCGTGGTACTGGCCG
AGGCATTCACCGGCTTCGGGCTCTTCCGGCAGCAGGCCTGGCTGGCCGCGCAGAACGCGCCGGAGTTGAGCGGCTTCGGC
GGCTCCGTCACGGTCGATGGCTTCGCCATCTTCTTCAACTGGATCTTCCTGGTCGCGGCCGTCGTAGTCGCGATTGTCTC
TTATAAGTATCTTGAGATCTCGGGCGAGCACCACGGTGAATACTACAGCCTGATTCTCTTCGCGCAGTGCGGGATGTACT
TCCTGGCCACCGGCACCGACCTGATCACGCTCTTCATCGGCCTCGAGCTGATGGCGCTCTGCTTCTACGTGATGGTCGGC
TTCCTGCGAACCGAGCGGCGCTCCAATGAGGCGGCGCTGAAATACCTACTGCTGGGGGCATTCTCGAGCGGCTTCCTGGT
TTACGGCTTCTCCGTGATGTACGGCATCGCGGGTTCCACCAAGCTCTCCGATATCGCCGCCGCCATTGCCAGCCGTCCAC
CGTGGGACCCGATGGTGTTCCTCGCGCTCTCCACGACCTCGGTCGGCCTGCTTTTCAAAGTCTCCGCCGTGCCCTTCCAC
ATGTGGGCTCCGGATGCTTACGAGGGCGCGCCCACCACCGTGACGGCGTATCTCTCGGTAGCTTCTAAGGCGGCATCGAT
CGCGTTCCTGCTGCGCATCTTCCTGGGGCCACTCGCCTCGGCGCGCGCGGTCTGGGAGCCGCTGCTGGCCTTTATCGCGA
TCATCACCCTGACCATCGGCAACCTGGCGGCGATCAATCAAACCAACATCAAGCGCCTCCTCGCCTACAGCTCGATTTCC
CACGCCGGTTACATGCTGCTCGGCCTGGTGGCCGGAAACGATACCGGGATCAAGGGCATTGCGGTCTACGTGATGGTCTA
CACCTTCATGAACCTGGGCGCCTTCCTGGTGATCATCGCGATGCGCCGCGCCAGCATCATCGGCGAAGATCTCGACGATC
TCGCCGGGCTGGTTCACAAGAGTCCCGGCTACGCGTTCCTGATGTTGATCTTCCTGCTCTCGCTGGCCGGCATTCCACCC
ACTGCGGGCTTCCTGGGCAAGTACTACATCTTCCTGGCGCTGATCCAGACCGGACATACCGGGCTCGCGATTGTGGCCAC
CCTTTACGTCGCGGTGGCGATTTACTACTACTTCAAGATCGTGCGCAGCATGTTCATCCGCGAGGAGATGGAGAAGACTC
CCATGCTCGCCACCAGCTTCGGCCTGCGCTGCGCGCTCGCCCTGACGGGCATCGCGACGCTGGCGATCGGCATTTATCCC
GAGCCGTTTCTCCGGCTCGCGCAGACTTCGTTGTTCCGGTAG

Upstream 100 bases:

>100_bases
TGCGGCCCGGATATTTTAGCGGCGCCGTGCAAAACGCGGGAGCGCGGCCCGCTGCCCCACCGGTGGCGCAGGCCCCGGCG
GTTGTCGCTGGAGGCGCGCA

Downstream 100 bases:

>100_bases
ACATATATGGAGACCGTTCTCAACCATCCGCTGTTCATGCCCGTCCTGGTGACGCTGGTCATCATTGCGGCCTTCCCGCT
GGTCGCCGGCTATATCGTGC

Product: proton-translocating NADH-quinone oxidoreductase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N 2; NDH-1 subunit N 2

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MIDFSLFYNSTDHFSIIPAVMLALFGCAILLFDFLIFPDPRQRKFLLIFVVLAEAFTGFGLFRQQAWLAAQNAPELSGFG
GSVTVDGFAIFFNWIFLVAAVVVAIVSYKYLEISGEHHGEYYSLILFAQCGMYFLATGTDLITLFIGLELMALCFYVMVG
FLRTERRSNEAALKYLLLGAFSSGFLVYGFSVMYGIAGSTKLSDIAAAIASRPPWDPMVFLALSTTSVGLLFKVSAVPFH
MWAPDAYEGAPTTVTAYLSVASKAASIAFLLRIFLGPLASARAVWEPLLAFIAIITLTIGNLAAINQTNIKRLLAYSSIS
HAGYMLLGLVAGNDTGIKGIAVYVMVYTFMNLGAFLVIIAMRRASIIGEDLDDLAGLVHKSPGYAFLMLIFLLSLAGIPP
TAGFLGKYYIFLALIQTGHTGLAIVATLYVAVAIYYYFKIVRSMFIREEMEKTPMLATSFGLRCALALTGIATLAIGIYP
EPFLRLAQTSLFR

Sequences:

>Translated_493_residues
MIDFSLFYNSTDHFSIIPAVMLALFGCAILLFDFLIFPDPRQRKFLLIFVVLAEAFTGFGLFRQQAWLAAQNAPELSGFG
GSVTVDGFAIFFNWIFLVAAVVVAIVSYKYLEISGEHHGEYYSLILFAQCGMYFLATGTDLITLFIGLELMALCFYVMVG
FLRTERRSNEAALKYLLLGAFSSGFLVYGFSVMYGIAGSTKLSDIAAAIASRPPWDPMVFLALSTTSVGLLFKVSAVPFH
MWAPDAYEGAPTTVTAYLSVASKAASIAFLLRIFLGPLASARAVWEPLLAFIAIITLTIGNLAAINQTNIKRLLAYSSIS
HAGYMLLGLVAGNDTGIKGIAVYVMVYTFMNLGAFLVIIAMRRASIIGEDLDDLAGLVHKSPGYAFLMLIFLLSLAGIPP
TAGFLGKYYIFLALIQTGHTGLAIVATLYVAVAIYYYFKIVRSMFIREEMEKTPMLATSFGLRCALALTGIATLAIGIYP
EPFLRLAQTSLFR
>Mature_493_residues
MIDFSLFYNSTDHFSIIPAVMLALFGCAILLFDFLIFPDPRQRKFLLIFVVLAEAFTGFGLFRQQAWLAAQNAPELSGFG
GSVTVDGFAIFFNWIFLVAAVVVAIVSYKYLEISGEHHGEYYSLILFAQCGMYFLATGTDLITLFIGLELMALCFYVMVG
FLRTERRSNEAALKYLLLGAFSSGFLVYGFSVMYGIAGSTKLSDIAAAIASRPPWDPMVFLALSTTSVGLLFKVSAVPFH
MWAPDAYEGAPTTVTAYLSVASKAASIAFLLRIFLGPLASARAVWEPLLAFIAIITLTIGNLAAINQTNIKRLLAYSSIS
HAGYMLLGLVAGNDTGIKGIAVYVMVYTFMNLGAFLVIIAMRRASIIGEDLDDLAGLVHKSPGYAFLMLIFLLSLAGIPP
TAGFLGKYYIFLALIQTGHTGLAIVATLYVAVAIYYYFKIVRSMFIREEMEKTPMLATSFGLRCALALTGIATLAIGIYP
EPFLRLAQTSLFR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831108, Length=303, Percent_Identity=22.4422442244224, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI145693160, Length=416, Percent_Identity=33.4134615384615, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1788614, Length=321, Percent_Identity=25.8566978193146, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788613, Length=262, Percent_Identity=26.7175572519084, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788831, Length=376, Percent_Identity=25.7978723404255, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788829, Length=356, Percent_Identity=25.561797752809, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788827, Length=278, Percent_Identity=24.4604316546763, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON2_SOLUE (Q01UN3)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_826922.1
- STRING:   Q01UN3
- GeneID:   4426100
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_5690
- NMPDR:   fig|234267.9.peg.5413
- HOGENOM:   HBG747830
- OMA:   MIMASAI
- PhylomeDB:   Q01UN3
- ProtClustDB:   CLSK774504
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 53824; Mature: 53824

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e159168)-; HASH(0x1d783944)-; HASH(0x1e61ce50)-; HASH(0x17e9588c)-; HASH(0x1e6009bc)-; HASH(0x1db6cb88)-; HASH(0x1e5f2de8)-; HASH(0x1d792a70)-; HASH(0x1e62ed6c)-; HASH(0x1dfaf29c)-; HASH(0x1e5f670c)-; HASH(0x1cefed9c)-; HASH(0x1e5f67fc)-; HASH(0x1ca4edc4)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFSLFYNSTDHFSIIPAVMLALFGCAILLFDFLIFPDPRQRKFLLIFVVLAEAFTGFG
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LFRQQAWLAAQNAPELSGFGGSVTVDGFAIFFNWIFLVAAVVVAIVSYKYLEISGEHHGE
HHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCH
YYSLILFAQCGMYFLATGTDLITLFIGLELMALCFYVMVGFLRTERRSNEAALKYLLLGA
HHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FSSGFLVYGFSVMYGIAGSTKLSDIAAAIASRPPWDPMVFLALSTTSVGLLFKVSAVPFH
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCEEE
MWAPDAYEGAPTTVTAYLSVASKAASIAFLLRIFLGPLASARAVWEPLLAFIAIITLTIG
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLAAINQTNIKRLLAYSSISHAGYMLLGLVAGNDTGIKGIAVYVMVYTFMNLGAFLVIIA
CHHCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MRRASIIGEDLDDLAGLVHKSPGYAFLMLIFLLSLAGIPPTAGFLGKYYIFLALIQTGHT
HHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
GLAIVATLYVAVAIYYYFKIVRSMFIREEMEKTPMLATSFGLRCALALTGIATLAIGIYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EPFLRLAQTSLFR
HHHHHHHHHHHCH
>Mature Secondary Structure
MIDFSLFYNSTDHFSIIPAVMLALFGCAILLFDFLIFPDPRQRKFLLIFVVLAEAFTGFG
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LFRQQAWLAAQNAPELSGFGGSVTVDGFAIFFNWIFLVAAVVVAIVSYKYLEISGEHHGE
HHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCH
YYSLILFAQCGMYFLATGTDLITLFIGLELMALCFYVMVGFLRTERRSNEAALKYLLLGA
HHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FSSGFLVYGFSVMYGIAGSTKLSDIAAAIASRPPWDPMVFLALSTTSVGLLFKVSAVPFH
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCEEE
MWAPDAYEGAPTTVTAYLSVASKAASIAFLLRIFLGPLASARAVWEPLLAFIAIITLTIG
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLAAINQTNIKRLLAYSSISHAGYMLLGLVAGNDTGIKGIAVYVMVYTFMNLGAFLVIIA
CHHCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MRRASIIGEDLDDLAGLVHKSPGYAFLMLIFLLSLAGIPPTAGFLGKYYIFLALIQTGHT
HHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
GLAIVATLYVAVAIYYYFKIVRSMFIREEMEKTPMLATSFGLRCALALTGIATLAIGIYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EPFLRLAQTSLFR
HHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA