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The map label for this gene is glpF [H]

Identifier: 116624630

GI number: 116624630

Start: 6960227

End: 6960940

Strand: Reverse

Name: glpF [H]

Synonym: Acid_5554

Alternate gene names: 116624630

Gene position: 6960940-6960227 (Counterclockwise)

Preceding gene: 116624631

Following gene: 116624627

Centisome position: 69.85

GC content: 65.13

Gene sequence:

>714_bases
ATGGGGACCTTCATCCTCATCCTGATGGGCAATGGTGTGGTCGCCAATGTCCTGTTGAAGAAGAGCAAGGCGGAAGGCGC
GGGCTGGATGGTGATCGCTACCGGATGGGCCTTCGCCGTGATGACCGGCATCTTCACCGCGGTGGCCTGCGGCAGCGCCG
ACGCGCACTTGAATCCCGCGGTGACGCTCGGCTTCGCGGTCAGCACCGGCGACTATTCCAAGCTGGCCGTCTATCTTCCC
GCGCAGCTGCTGGGCGCGATGTGCGGAGCGCTTCTCGTGTGGCTCTACTTCCTGCCGCACTGGGCCGTCACGGAAGATCG
CGACATCAAGCTGGGATGCTTCGCCACTTCGCCCGCGATCAGCAACTTCGGTTCGAACCTGTTCAGCGAGGCCATCGGAA
CCTTCGTGCTCATCACCGTGGTGGCCGCGATCACCTCCAAGGCGGTCGGCAACCTGGGACCCGCCGGCGCCTTGTCCCCC
GGCTTCGGACCATACCTCGTTGGCATGCTGGTCTGGTCGATCGGCCTTTCGCTCGGCGGCACCACCGGCTACGCGATCAA
CCCTGCGCGCGACCTGGGACCGCGCATCATGCACGCCATCCTCCCGGTCGCGGGCAAAGGCGGTTCGAATTGGGGCTACG
CTCCAGTGCCCATCCTCGGGCCCATGATCGGCGCCGCGCTGGCCGGCATCTTCATCAAGTCCGCCGGATTCTAA

Upstream 100 bases:

>100_bases
GCGGCGGCAGGAGTTGTACAGCTTTTGGAAGAAGGCGGTTACCCGGTCGTTCGACTGGCTGTAGAGAGCGCTTATGCCAT
CCAAATTTTTCGGTGAGTTC

Downstream 100 bases:

>100_bases
ACTGCTGTCAGAGCCGCGACTGTCAGGGAGCGATCACCGGCGTTAATTCTCGCTCGGCCGCTCCACGTGATCGATCACCA
GCACTTCCACCGGACCCTTC

Product: major intrinsic protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MGTFILILMGNGVVANVLLKKSKAEGAGWMVIATGWAFAVMTGIFTAVACGSADAHLNPAVTLGFAVSTGDYSKLAVYLP
AQLLGAMCGALLVWLYFLPHWAVTEDRDIKLGCFATSPAISNFGSNLFSEAIGTFVLITVVAAITSKAVGNLGPAGALSP
GFGPYLVGMLVWSIGLSLGGTTGYAINPARDLGPRIMHAILPVAGKGGSNWGYAPVPILGPMIGAALAGIFIKSAGF

Sequences:

>Translated_237_residues
MGTFILILMGNGVVANVLLKKSKAEGAGWMVIATGWAFAVMTGIFTAVACGSADAHLNPAVTLGFAVSTGDYSKLAVYLP
AQLLGAMCGALLVWLYFLPHWAVTEDRDIKLGCFATSPAISNFGSNLFSEAIGTFVLITVVAAITSKAVGNLGPAGALSP
GFGPYLVGMLVWSIGLSLGGTTGYAINPARDLGPRIMHAILPVAGKGGSNWGYAPVPILGPMIGAALAGIFIKSAGF
>Mature_236_residues
GTFILILMGNGVVANVLLKKSKAEGAGWMVIATGWAFAVMTGIFTAVACGSADAHLNPAVTLGFAVSTGDYSKLAVYLPA
QLLGAMCGALLVWLYFLPHWAVTEDRDIKLGCFATSPAISNFGSNLFSEAIGTFVLITVVAAITSKAVGNLGPAGALSPG
FGPYLVGMLVWSIGLSLGGTTGYAINPARDLGPRIMHAILPVAGKGGSNWGYAPVPILGPMIGAALAGIFIKSAGF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=248, Percent_Identity=36.2903225806452, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI157266307, Length=243, Percent_Identity=34.156378600823, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI4502187, Length=247, Percent_Identity=34.0080971659919, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI22538420, Length=241, Percent_Identity=30.7053941908714, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1790362, Length=240, Percent_Identity=34.1666666666667, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994009, Length=244, Percent_Identity=30.327868852459, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17544068, Length=241, Percent_Identity=33.6099585062241, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17531431, Length=238, Percent_Identity=28.9915966386555, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17531429, Length=238, Percent_Identity=28.9915966386555, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17533613, Length=245, Percent_Identity=29.7959183673469, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32564052, Length=247, Percent_Identity=29.5546558704453, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=212, Percent_Identity=31.1320754716981, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322985, Length=211, Percent_Identity=32.2274881516588, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24164; Mature: 24033

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTFILILMGNGVVANVLLKKSKAEGAGWMVIATGWAFAVMTGIFTAVACGSADAHLNPA
CCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCE
VTLGFAVSTGDYSKLAVYLPAQLLGAMCGALLVWLYFLPHWAVTEDRDIKLGCFATSPAI
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHH
SNFGSNLFSEAIGTFVLITVVAAITSKAVGNLGPAGALSPGFGPYLVGMLVWSIGLSLGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TTGYAINPARDLGPRIMHAILPVAGKGGSNWGYAPVPILGPMIGAALAGIFIKSAGF
CCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GTFILILMGNGVVANVLLKKSKAEGAGWMVIATGWAFAVMTGIFTAVACGSADAHLNPA
CEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCE
VTLGFAVSTGDYSKLAVYLPAQLLGAMCGALLVWLYFLPHWAVTEDRDIKLGCFATSPAI
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHH
SNFGSNLFSEAIGTFVLITVVAAITSKAVGNLGPAGALSPGFGPYLVGMLVWSIGLSLGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TTGYAINPARDLGPRIMHAILPVAGKGGSNWGYAPVPILGPMIGAALAGIFIKSAGF
CCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]