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The map label for this gene is glpK

Identifier: 116624631

GI number: 116624631

Start: 6960977

End: 6962461

Strand: Reverse

Name: glpK

Synonym: Acid_5555

Alternate gene names: 116624631

Gene position: 6962461-6960977 (Counterclockwise)

Preceding gene: 116624635

Following gene: 116624630

Centisome position: 69.86

GC content: 65.32

Gene sequence:

>1485_bases
TTGTCCAACTACATCGGCGCCATCGACCAAGGCACCACCAGCACGCGATTCATCGTGTTCGACCACGGGGGCCGGATCGT
CTCCGTCGCCCAGAAAGAGCACGAGCAGATCTTCCCCCAGCCCGGCTGGGTGGAGCATGACGCCAACGAGATCTGGCGCC
GCACCCGCGAAGTAATCGCCGAGGCGCTCGAGAAGCGCGCCCTCTCCGCCTCCGACCTCGCCGCCATCGGCATCACCAAC
CAGCGCGAAACCACCGTGGTGTGGGACCGCCTTAGCGGCGAGCCCGTGTACAACGCCCTCGTCTGGCAGGATACGCGCAC
CGCGGCCGCCGTGGCCGAGCTCGCCCGTGACGGCGGCGCCGACCGCTTCCGCGCCCAGACCGGACTTCCGCTTGCCACCT
ACTTCAGCGCCCTCAAGATCGGCTGGATCCTCGACAACGTGCCCGCCGTCCGCGCGCGCGCCGAAGCAGGCGACATCCTC
TTCGGCAACATCGACACCTTCCTGCTGTGGAACCTCACCGGCGGCCTGCACCTCACCGATTGCACCAACGCCAGCCGCAC
TCACCTGATGAACCTGCAGACGCTGGATTGGGACGACGAGCTGCTCGCTGCCTTCCGCATTCCCCGCGCCATGCTGCCGC
GCATCGTCTCCAGCAGCGAGGTCTACGGCAACGCAACTCTTGCCTCCGTGGCCGGCGTCCCGATCGCGGGCATCCTCGGC
GACCAGCAGGCGGCACTCGTGGGCCAGACCTGTTTTCACGCGGGCGAGGCCAAAAACACGTATGGCACCGGATGTTTCCT
GCTGATGAACACCGGAACCAAGCCCACGCCTTCGAAACACGGCATGTTAACCACCGTGGCCTATCGCTTCGCCACGCAGC
CCGCTGTCTATGCGCTCGAAGGCAGCGTCGCCATCACGGGCGCATTGGTGCAATGGGTCCGCGACAATTTCGGCCTGATC
GAAAAGAGCAGCGATATCGAAGTGCTCGCGCGCACTGTCAAGGATAACGGCGGCGTATACTTCGTCCCCGCCTTCAGCGG
CCTTTACGCGCCCTACTGGAAGGACAACGCGCGCGGCGTGATCGCCGGCCTCACGCGCTATACCAACAAAGGCCACCTGG
CGCGCGCCGTCCTCGAAGCCACCGCTTTCCAGACGCGCGAAGTAGTGGAAGCGATGGAGAAGGATTCCGCCATCGCGCTC
GAGACCCTGCGCACCGATGGGGGCATGGTCTCTAACGACCTGCTCATGCAGTTCCAGTCCGATATACTGGATCGCCCCGT
GGTGCGTCCCGCGGTGCAGGAAACCACGGCTCTCGGCGCTGCCTACGCGGCCGGCCTGGCGGTGGGATTCTTCCAGGACA
CCGACGACCTGCGCAGCCGGTGGAGCGTGGATCATACCTGGAAGCCGCAGATGGAGCCCGAGCGGCGGCAGGAGTTGTAC
AGCTTTTGGAAGAAGGCGGTTACCCGGTCGTTCGACTGGCTGTAG

Upstream 100 bases:

>100_bases
TCTCTAGCGCCCTCGACGGCATTAGGACACACCCCTCCGATAGCGAAAACCCTCCGAAGCCGCTACACTATTTCGTTACG
GAAAGTGAGAGTGACCTCGT

Downstream 100 bases:

>100_bases
AGAGCGCTTATGCCATCCAAATTTTTCGGTGAGTTCATGGGGACCTTCATCCTCATCCTGATGGGCAATGGTGTGGTCGC
CAATGTCCTGTTGAAGAAGA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSNYIGAIDQGTTSTRFIVFDHGGRIVSVAQKEHEQIFPQPGWVEHDANEIWRRTREVIAEALEKRALSASDLAAIGITN
QRETTVVWDRLSGEPVYNALVWQDTRTAAAVAELARDGGADRFRAQTGLPLATYFSALKIGWILDNVPAVRARAEAGDIL
FGNIDTFLLWNLTGGLHLTDCTNASRTHLMNLQTLDWDDELLAAFRIPRAMLPRIVSSSEVYGNATLASVAGVPIAGILG
DQQAALVGQTCFHAGEAKNTYGTGCFLLMNTGTKPTPSKHGMLTTVAYRFATQPAVYALEGSVAITGALVQWVRDNFGLI
EKSSDIEVLARTVKDNGGVYFVPAFSGLYAPYWKDNARGVIAGLTRYTNKGHLARAVLEATAFQTREVVEAMEKDSAIAL
ETLRTDGGMVSNDLLMQFQSDILDRPVVRPAVQETTALGAAYAAGLAVGFFQDTDDLRSRWSVDHTWKPQMEPERRQELY
SFWKKAVTRSFDWL

Sequences:

>Translated_494_residues
MSNYIGAIDQGTTSTRFIVFDHGGRIVSVAQKEHEQIFPQPGWVEHDANEIWRRTREVIAEALEKRALSASDLAAIGITN
QRETTVVWDRLSGEPVYNALVWQDTRTAAAVAELARDGGADRFRAQTGLPLATYFSALKIGWILDNVPAVRARAEAGDIL
FGNIDTFLLWNLTGGLHLTDCTNASRTHLMNLQTLDWDDELLAAFRIPRAMLPRIVSSSEVYGNATLASVAGVPIAGILG
DQQAALVGQTCFHAGEAKNTYGTGCFLLMNTGTKPTPSKHGMLTTVAYRFATQPAVYALEGSVAITGALVQWVRDNFGLI
EKSSDIEVLARTVKDNGGVYFVPAFSGLYAPYWKDNARGVIAGLTRYTNKGHLARAVLEATAFQTREVVEAMEKDSAIAL
ETLRTDGGMVSNDLLMQFQSDILDRPVVRPAVQETTALGAAYAAGLAVGFFQDTDDLRSRWSVDHTWKPQMEPERRQELY
SFWKKAVTRSFDWL
>Mature_493_residues
SNYIGAIDQGTTSTRFIVFDHGGRIVSVAQKEHEQIFPQPGWVEHDANEIWRRTREVIAEALEKRALSASDLAAIGITNQ
RETTVVWDRLSGEPVYNALVWQDTRTAAAVAELARDGGADRFRAQTGLPLATYFSALKIGWILDNVPAVRARAEAGDILF
GNIDTFLLWNLTGGLHLTDCTNASRTHLMNLQTLDWDDELLAAFRIPRAMLPRIVSSSEVYGNATLASVAGVPIAGILGD
QQAALVGQTCFHAGEAKNTYGTGCFLLMNTGTKPTPSKHGMLTTVAYRFATQPAVYALEGSVAITGALVQWVRDNFGLIE
KSSDIEVLARTVKDNGGVYFVPAFSGLYAPYWKDNARGVIAGLTRYTNKGHLARAVLEATAFQTREVVEAMEKDSAIALE
TLRTDGGMVSNDLLMQFQSDILDRPVVRPAVQETTALGAAYAAGLAVGFFQDTDDLRSRWSVDHTWKPQMEPERRQELYS
FWKKAVTRSFDWL

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI41393575, Length=510, Percent_Identity=51.9607843137255, Blast_Score=509, Evalue=1e-144,
Organism=Homo sapiens, GI4504007, Length=504, Percent_Identity=52.5793650793651, Blast_Score=508, Evalue=1e-144,
Organism=Homo sapiens, GI189409120, Length=504, Percent_Identity=52.5793650793651, Blast_Score=507, Evalue=1e-144,
Organism=Homo sapiens, GI42794763, Length=510, Percent_Identity=51.9607843137255, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=31.8271119842829, Blast_Score=287, Evalue=2e-77,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=53.9393939393939, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1790005, Length=470, Percent_Identity=25.531914893617, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789168, Length=490, Percent_Identity=25.7142857142857, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787789, Length=507, Percent_Identity=27.2189349112426, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789987, Length=482, Percent_Identity=25.103734439834, Blast_Score=99, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17535599, Length=499, Percent_Identity=45.2905811623246, Blast_Score=438, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71986140, Length=526, Percent_Identity=24.9049429657795, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71986147, Length=261, Percent_Identity=31.4176245210728, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321755, Length=553, Percent_Identity=39.2405063291139, Blast_Score=344, Evalue=2e-95,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=48.4189723320158, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24655555, Length=502, Percent_Identity=44.4223107569721, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24655560, Length=502, Percent_Identity=44.4223107569721, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24655570, Length=502, Percent_Identity=44.4223107569721, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24655574, Length=502, Percent_Identity=44.4223107569721, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24655565, Length=502, Percent_Identity=44.4223107569721, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24654573, Length=434, Percent_Identity=49.7695852534562, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI19922058, Length=531, Percent_Identity=35.0282485875706, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=35.8452138492872, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=31.2015503875969, Blast_Score=222, Evalue=5e-58,
Organism=Drosophila melanogaster, GI21357867, Length=528, Percent_Identity=29.9242424242424, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24656372, Length=329, Percent_Identity=32.5227963525836, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45552909, Length=270, Percent_Identity=31.4814814814815, Blast_Score=128, Evalue=9e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_SOLUE (Q01V13)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_826787.1
- ProteinModelPortal:   Q01V13
- SMR:   Q01V13
- STRING:   Q01V13
- GeneID:   4426210
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_5555
- NMPDR:   fig|234267.9.peg.5283
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   ATYFSGT
- PhylomeDB:   Q01V13
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54311; Mature: 54180

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 241-241 BINDING 263-263 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYIGAIDQGTTSTRFIVFDHGGRIVSVAQKEHEQIFPQPGWVEHDANEIWRRTREVIA
CCCCCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EALEKRALSASDLAAIGITNQRETTVVWDRLSGEPVYNALVWQDTRTAAAVAELARDGGA
HHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCCCHHEEEEECCCHHHHHHHHHHHCCCC
DRFRAQTGLPLATYFSALKIGWILDNVPAVRARAEAGDILFGNIDTFLLWNLTGGLHLTD
CHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCEEEEEEECCCEEEEE
CTNASRTHLMNLQTLDWDDELLAAFRIPRAMLPRIVSSSEVYGNATLASVAGVPIAGILG
CCCCCHHEEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECC
DQQAALVGQTCFHAGEAKNTYGTGCFLLMNTGTKPTPSKHGMLTTVAYRFATQPAVYALE
CCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEC
GSVAITGALVQWVRDNFGLIEKSSDIEVLARTVKDNGGVYFVPAFSGLYAPYWKDNARGV
CCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEE
IAGLTRYTNKGHLARAVLEATAFQTREVVEAMEKDSAIALETLRTDGGMVSNDLLMQFQS
EHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCHHHHHHHHHH
DILDRPVVRPAVQETTALGAAYAAGLAVGFFQDTDDLRSRWSVDHTWKPQMEPERRQELY
HHHCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHH
SFWKKAVTRSFDWL
HHHHHHHHHCCCCC
>Mature Secondary Structure 
SNYIGAIDQGTTSTRFIVFDHGGRIVSVAQKEHEQIFPQPGWVEHDANEIWRRTREVIA
CCCCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EALEKRALSASDLAAIGITNQRETTVVWDRLSGEPVYNALVWQDTRTAAAVAELARDGGA
HHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCCCHHEEEEECCCHHHHHHHHHHHCCCC
DRFRAQTGLPLATYFSALKIGWILDNVPAVRARAEAGDILFGNIDTFLLWNLTGGLHLTD
CHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCEEEEEEECCCEEEEE
CTNASRTHLMNLQTLDWDDELLAAFRIPRAMLPRIVSSSEVYGNATLASVAGVPIAGILG
CCCCCHHEEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECC
DQQAALVGQTCFHAGEAKNTYGTGCFLLMNTGTKPTPSKHGMLTTVAYRFATQPAVYALE
CCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEC
GSVAITGALVQWVRDNFGLIEKSSDIEVLARTVKDNGGVYFVPAFSGLYAPYWKDNARGV
CCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEE
IAGLTRYTNKGHLARAVLEATAFQTREVVEAMEKDSAIALETLRTDGGMVSNDLLMQFQS
EHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCHHHHHHHHHH
DILDRPVVRPAVQETTALGAAYAAGLAVGFFQDTDDLRSRWSVDHTWKPQMEPERRQELY
HHHCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHH
SFWKKAVTRSFDWL
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA