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The map label for this gene is cusS [H]

Identifier: 116624554

GI number: 116624554

Start: 6870962

End: 6872272

Strand: Direct

Name: cusS [H]

Synonym: Acid_5478

Alternate gene names: 116624554

Gene position: 6870962-6872272 (Clockwise)

Preceding gene: 116624553

Following gene: 116624559

Centisome position: 68.95

GC content: 64.84

Gene sequence:

>1311_bases
ATGTTTAAGAGCCTCCGCGCGCGCCTGACAGCGCTTTACCTGGTGCTGTTCTCGCTGTTGTTCGTCCTATTCAGCCTGTC
CCTGTACGGTGAACTCTCGCGGTCTTTGCTTTTGCGGCTGGACCAGACCCTGGCATCGGAGGTGGATACCGCCGCGTTCC
TGTTCCCCGACGAATTCCAGGAGATGAGGGGCGACGCGCTGCTGGCGGCCCGCGAAGTGGTCAACGAAATGAAGGTGCAC
GGCGATCTGGTCACGGTGCGGGAGGGCGGAGAGGTGCTGGCCTCCAGTGGTCAACCCGCGCCAGGCGCGCGCACACGAGG
CGCATCGCGCATGGCCGAGGCCGGGGGCCGGAAGTTCCAGATTGAGATTGTGGCCTCGCTCGATTCGATCGATGCCGAAC
TGGGAGTGGTGCGCCGGGTGATCTTCATCGGGCTGCCGCTGGTGCTGGCCCTGGCCGGTTTCGGCGGCTACCTGCTGGCC
ACACGCACGCTGCGGCCGCTGGCTTCGATGGCCGAACAGGCGCAGCGCATCACGGGCACCAATTTGGAAACCCGCATTCA
GGTAGAGAACGCCGCTTCGGAACTGGCCGTGCTGGTGACCTCCTTCAACGAGTTGCTTTCGCGCCTCGACCACTCGTTCG
ATACCATGCGACGGTTCGTCGCCGACGCTTCCCACGAGTTGCGGACGCCGATCTCGGTGATCCGCGGGGAGGCCGACGTG
GCGCTCTCGCAGGAGCGCGGGAATGGCGAGTACCGCGAGTCGCTGGGCATCGTGCTGGATGAATCGCGGCGCCTCTCGCG
GCTGGTGGACGATTTGTTGAACCTGGCGCGCGCCGATGCCGGCCACGTCAAACTGCAGACCCACGATTTTTACCTGAATG
AGTTAGTGACGGAATGCTGCCGGTCCGTGCAGGGCCTGGCCGGCACGCGGCAACTCACGCTCGAATGCCGAGCGGGCAGC
GACCTCCAGTTCACGGGCGACGAGCAACTGCTGCGCCGGCTCGTGATCAATTTGCTCGACAATGCGATTCGCTATACGCC
GGTGGGCGGCAGCGTGACTGCCTCGCTGGAGGCGGGTCCGGAGAGCGTATCGCTGCGCGTTTCCGATACCGGAATCGGCA
TCGCACCGGGAGATGCGGCACGCGTTTTCGAACGCTTCTACCGCGCCGATGAGGCACGCTCGCGGCAGGACGGCGGTTTC
GGGCTTGGCCTGGCGATCGTAAAGTGGATCGCCGAGTCGCACCGCGGCATGGTGGAATGCTCGAGCCAGCAGGGTTCGGG
AAGTTGCTTCACGGTGAGGCTCCCGCGGTAA

Upstream 100 bases:

>100_bases
AAGAGGCTGCGCGCCAAGCTCGATGGGCCGGGATCGCCGCGGCTGATCCACACGCGCCGGGGCGAGGGCTACATCCTCAC
GGCGAAGCCGGAGGCCGGCG

Downstream 100 bases:

>100_bases
GAGGCGAGTGGTTCATGGTCTTATGTGGTACCGCTCTGGCTGACAGCGACTACAGTTCCGTGCCCGATGGAATCAAGCGC
ATCGTTTGCAGGCTCGAACG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MFKSLRARLTALYLVLFSLLFVLFSLSLYGELSRSLLLRLDQTLASEVDTAAFLFPDEFQEMRGDALLAAREVVNEMKVH
GDLVTVREGGEVLASSGQPAPGARTRGASRMAEAGGRKFQIEIVASLDSIDAELGVVRRVIFIGLPLVLALAGFGGYLLA
TRTLRPLASMAEQAQRITGTNLETRIQVENAASELAVLVTSFNELLSRLDHSFDTMRRFVADASHELRTPISVIRGEADV
ALSQERGNGEYRESLGIVLDESRRLSRLVDDLLNLARADAGHVKLQTHDFYLNELVTECCRSVQGLAGTRQLTLECRAGS
DLQFTGDEQLLRRLVINLLDNAIRYTPVGGSVTASLEAGPESVSLRVSDTGIGIAPGDAARVFERFYRADEARSRQDGGF
GLGLAIVKWIAESHRGMVECSSQQGSGSCFTVRLPR

Sequences:

>Translated_436_residues
MFKSLRARLTALYLVLFSLLFVLFSLSLYGELSRSLLLRLDQTLASEVDTAAFLFPDEFQEMRGDALLAAREVVNEMKVH
GDLVTVREGGEVLASSGQPAPGARTRGASRMAEAGGRKFQIEIVASLDSIDAELGVVRRVIFIGLPLVLALAGFGGYLLA
TRTLRPLASMAEQAQRITGTNLETRIQVENAASELAVLVTSFNELLSRLDHSFDTMRRFVADASHELRTPISVIRGEADV
ALSQERGNGEYRESLGIVLDESRRLSRLVDDLLNLARADAGHVKLQTHDFYLNELVTECCRSVQGLAGTRQLTLECRAGS
DLQFTGDEQLLRRLVINLLDNAIRYTPVGGSVTASLEAGPESVSLRVSDTGIGIAPGDAARVFERFYRADEARSRQDGGF
GLGLAIVKWIAESHRGMVECSSQQGSGSCFTVRLPR
>Mature_436_residues
MFKSLRARLTALYLVLFSLLFVLFSLSLYGELSRSLLLRLDQTLASEVDTAAFLFPDEFQEMRGDALLAAREVVNEMKVH
GDLVTVREGGEVLASSGQPAPGARTRGASRMAEAGGRKFQIEIVASLDSIDAELGVVRRVIFIGLPLVLALAGFGGYLLA
TRTLRPLASMAEQAQRITGTNLETRIQVENAASELAVLVTSFNELLSRLDHSFDTMRRFVADASHELRTPISVIRGEADV
ALSQERGNGEYRESLGIVLDESRRLSRLVDDLLNLARADAGHVKLQTHDFYLNELVTECCRSVQGLAGTRQLTLECRAGS
DLQFTGDEQLLRRLVINLLDNAIRYTPVGGSVTASLEAGPESVSLRVSDTGIGIAPGDAARVFERFYRADEARSRQDGGF
GLGLAIVKWIAESHRGMVECSSQQGSGSCFTVRLPR

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=369, Percent_Identity=32.2493224932249, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1786600, Length=291, Percent_Identity=32.6460481099656, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1788393, Length=272, Percent_Identity=32.7205882352941, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788279, Length=253, Percent_Identity=30.8300395256917, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=29.6703296703297, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790551, Length=299, Percent_Identity=31.7725752508361, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI145693157, Length=272, Percent_Identity=29.4117647058824, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=33.9366515837104, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789403, Length=290, Percent_Identity=32.4137931034483, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787894, Length=298, Percent_Identity=27.5167785234899, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789149, Length=222, Percent_Identity=31.0810810810811, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786912, Length=302, Percent_Identity=30.4635761589404, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081816, Length=253, Percent_Identity=29.2490118577075, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788713, Length=225, Percent_Identity=28, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790861, Length=247, Percent_Identity=32.3886639676113, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789808, Length=288, Percent_Identity=27.0833333333333, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=26.0089686098655, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI48994928, Length=222, Percent_Identity=27.4774774774775, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082128, Length=223, Percent_Identity=26.9058295964126, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787374, Length=320, Percent_Identity=24.375, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47549; Mature: 47549

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKSLRARLTALYLVLFSLLFVLFSLSLYGELSRSLLLRLDQTLASEVDTAAFLFPDEFQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCHHHH
EMRGDALLAAREVVNEMKVHGDLVTVREGGEVLASSGQPAPGARTRGASRMAEAGGRKFQ
HHCCHHHHHHHHHHHHHHHCCCEEEEECCCHHEECCCCCCCCCCCCCHHHHHHCCCCEEE
IEIVASLDSIDAELGVVRRVIFIGLPLVLALAGFGGYLLATRTLRPLASMAEQAQRITGT
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
NLETRIQVENAASELAVLVTSFNELLSRLDHSFDTMRRFVADASHELRTPISVIRGEADV
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCHHHHCCCCCE
ALSQERGNGEYRESLGIVLDESRRLSRLVDDLLNLARADAGHVKLQTHDFYLNELVTECC
EEECCCCCCHHHHHCCCEECCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
RSVQGLAGTRQLTLECRAGSDLQFTGDEQLLRRLVINLLDNAIRYTPVGGSVTASLEAGP
HHHHCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCC
ESVSLRVSDTGIGIAPGDAARVFERFYRADEARSRQDGGFGLGLAIVKWIAESHRGMVEC
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEE
SSQQGSGSCFTVRLPR
CCCCCCCCEEEEECCC
>Mature Secondary Structure
MFKSLRARLTALYLVLFSLLFVLFSLSLYGELSRSLLLRLDQTLASEVDTAAFLFPDEFQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCHHHH
EMRGDALLAAREVVNEMKVHGDLVTVREGGEVLASSGQPAPGARTRGASRMAEAGGRKFQ
HHCCHHHHHHHHHHHHHHHCCCEEEEECCCHHEECCCCCCCCCCCCCHHHHHHCCCCEEE
IEIVASLDSIDAELGVVRRVIFIGLPLVLALAGFGGYLLATRTLRPLASMAEQAQRITGT
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
NLETRIQVENAASELAVLVTSFNELLSRLDHSFDTMRRFVADASHELRTPISVIRGEADV
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCHHHHCCCCCE
ALSQERGNGEYRESLGIVLDESRRLSRLVDDLLNLARADAGHVKLQTHDFYLNELVTECC
EEECCCCCCHHHHHCCCEECCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
RSVQGLAGTRQLTLECRAGSDLQFTGDEQLLRRLVINLLDNAIRYTPVGGSVTASLEAGP
HHHHCCCCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCC
ESVSLRVSDTGIGIAPGDAARVFERFYRADEARSRQDGGFGLGLAIVKWIAESHRGMVEC
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEE
SSQQGSGSCFTVRLPR
CCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]