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The map label for this gene is truA

Identifier: 116624080

GI number: 116624080

Start: 6301387

End: 6302109

Strand: Direct

Name: truA

Synonym: Acid_4994

Alternate gene names: 116624080

Gene position: 6301387-6302109 (Clockwise)

Preceding gene: 116624079

Following gene: 116624081

Centisome position: 63.23

GC content: 63.62

Gene sequence:

>723_bases
ATGCGGCGCATCCGCATTTATCTCGCCTACGATGGCGGACCGTTTCACGGGTGGCAGGTGCAGCCCGGCCTCGCCACCAT
CCAGGGCATTCTCGAGGAAATCGTCGGCGGCATGGAAGGCAAGCCCGTCCACGTCGCCGGCAGCGGCCGCACCGACGCCG
GCGTCCACGCACTCGCTCAGGTCGCCGCCTTCAGCATCGAAAATCCCATACCGGTCGAGAACCTCCGCCGCGCCGTCAAT
CGCCTGCTTCCCCCGGCCATCCGAATCCTCTGCGCCGAGGAGGTGCAGGCCGATTTTCATCCGCGCTTCGACGCCGTGGC
CAAGACTTACGAGTACCGCATGTTCCGCGACGAAGTATGTAGCCCTTTCGAGTGGCCGTATGTGTATCACCACCCCTACC
CGCTGGATGAAGCACGCATGGCAACGCTCGCACGCGCTTTCGAAGGCGAGCACGATTTCACCCCATTCGCCGCCTCCGAT
GCCCGCGATGCCGAAGGCAAGTCCAAAGTGCGCACCATCTTCTCTTCCACCCTGGACCGCACCCCGCAAAGACTCGTCTA
CCGCGTGCGGGGCAGCGGTTTTCTCAAACACATGGTTCGCAATATCGTCGGCACCCTGATCGAAGCCGGTCGCGGCAACA
TCGCGGACCTCAATTCCCTGCCCGCCCGCTCCGGCGCCACCGCCCCCGCCAAAGGTCTCTTTCAGGTCAGCGTGGAGTAT
TAA

Upstream 100 bases:

>100_bases
TGGCACTGGCCACTCTGCAGGTGGCGGCTCATCGCCCTGAGGATTGCCCGCTGTGCCGGCAGGGCCTGCCCGTAGTCAAG
CCCGGCTCGCGCCCGGCGTA

Downstream 100 bases:

>100_bases
ATGGAAGCCCCCGAAATTCTCGCCGCTTTGCGCACGCAGGCGAACCCCGTCGACGTCGCCGGCATGGCGCGCTACGGCAT
CAGCACCAATGGCACGCTCG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRRIRIYLAYDGGPFHGWQVQPGLATIQGILEEIVGGMEGKPVHVAGSGRTDAGVHALAQVAAFSIENPIPVENLRRAVN
RLLPPAIRILCAEEVQADFHPRFDAVAKTYEYRMFRDEVCSPFEWPYVYHHPYPLDEARMATLARAFEGEHDFTPFAASD
ARDAEGKSKVRTIFSSTLDRTPQRLVYRVRGSGFLKHMVRNIVGTLIEAGRGNIADLNSLPARSGATAPAKGLFQVSVEY

Sequences:

>Translated_240_residues
MRRIRIYLAYDGGPFHGWQVQPGLATIQGILEEIVGGMEGKPVHVAGSGRTDAGVHALAQVAAFSIENPIPVENLRRAVN
RLLPPAIRILCAEEVQADFHPRFDAVAKTYEYRMFRDEVCSPFEWPYVYHHPYPLDEARMATLARAFEGEHDFTPFAASD
ARDAEGKSKVRTIFSSTLDRTPQRLVYRVRGSGFLKHMVRNIVGTLIEAGRGNIADLNSLPARSGATAPAKGLFQVSVEY
>Mature_240_residues
MRRIRIYLAYDGGPFHGWQVQPGLATIQGILEEIVGGMEGKPVHVAGSGRTDAGVHALAQVAAFSIENPIPVENLRRAVN
RLLPPAIRILCAEEVQADFHPRFDAVAKTYEYRMFRDEVCSPFEWPYVYHHPYPLDEARMATLARAFEGEHDFTPFAASD
ARDAEGKSKVRTIFSSTLDRTPQRLVYRVRGSGFLKHMVRNIVGTLIEAGRGNIADLNSLPARSGATAPAKGLFQVSVEY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=33.8775510204082, Blast_Score=128, Evalue=3e-31,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=28.476821192053, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281362146, Length=230, Percent_Identity=26.9565217391304, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648455, Length=234, Percent_Identity=26.4957264957265, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_SOLUE (Q01WL4)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_826236.1
- ProteinModelPortal:   Q01WL4
- SMR:   Q01WL4
- STRING:   Q01WL4
- GeneID:   4426777
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_4994
- NMPDR:   fig|234267.9.peg.4739
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q01WL4
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 26636; Mature: 26636

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIRIYLAYDGGPFHGWQVQPGLATIQGILEEIVGGMEGKPVHVAGSGRTDAGVHALAQ
CCEEEEEEEECCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHH
VAAFSIENPIPVENLRRAVNRLLPPAIRILCAEEVQADFHPRFDAVAKTYEYRMFRDEVC
HHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
SPFEWPYVYHHPYPLDEARMATLARAFEGEHDFTPFAASDARDAEGKSKVRTIFSSTLDR
CCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
TPQRLVYRVRGSGFLKHMVRNIVGTLIEAGRGNIADLNSLPARSGATAPAKGLFQVSVEY
CHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRRIRIYLAYDGGPFHGWQVQPGLATIQGILEEIVGGMEGKPVHVAGSGRTDAGVHALAQ
CCEEEEEEEECCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHH
VAAFSIENPIPVENLRRAVNRLLPPAIRILCAEEVQADFHPRFDAVAKTYEYRMFRDEVC
HHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
SPFEWPYVYHHPYPLDEARMATLARAFEGEHDFTPFAASDARDAEGKSKVRTIFSSTLDR
CCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
TPQRLVYRVRGSGFLKHMVRNIVGTLIEAGRGNIADLNSLPARSGATAPAKGLFQVSVEY
CHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA