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The map label for this gene is yfkE [H]

Identifier: 116624025

GI number: 116624025

Start: 6232916

End: 6233980

Strand: Direct

Name: yfkE [H]

Synonym: Acid_4937

Alternate gene names: 116624025

Gene position: 6232916-6233980 (Clockwise)

Preceding gene: 116624024

Following gene: 116624026

Centisome position: 62.54

GC content: 65.07

Gene sequence:

>1065_bases
ATGCTGAACTGGCTGCTGATCTTCGTGCCGGTAGCGATCGGGCTCGAGTTTTTCACGCCCGGGCAGCACACGTTGATCTT
CATTACCGCCTGCCTAGCCGTTATCCCGCTGGCCGGCTGGCTCGGCCACGCAACCGAGGAACTGGCGCGCTACACCGGCG
AAGGCGCGGGCGGCTTACTCAATGCAACCTTCGGCAACGCCGCCGAGCTCATCATCGCCTTCATGGCGATTCGCAAGGGG
CTCTACTCCGTCGTCAAAGCGTCGCTCACGGGAAGCATCATCGGCAACATCCTGCTCGTGCTCGGCGCCGCCGTGCTCGC
CGGCGGCCTCAAGCACAAGCACCAGCGCTTCAACGTCACCGGCGCGCGCGCCCAGGCCACGCTGCTCACGCTCGCCGCCA
TCGCGCTGGTCATGCCCGCCGCCTTCCACGCGCTCGCCGGGCCGCCCAACATCGTGAAGGAGGATGGCCTCAGCCTTGCG
ATCGCCTGCGTCCTGCTGATCGCTTACGGACTCCACCTGCTCTTCTCACTGCGCACTCACAAACAGCTTTTCGAAGGAGA
GCCCGAGCATCAGGAGGAGCCTGAGCCCGCCGCGTGGAGCCTCAAGCGCGCGCTCCTCACGCTCGCGGGGGCCACGCTCG
TGATCGCCTGGGTCAGCGAGATCCTGGTCGGCTCGGTGGAGCAGACCGCCGCCGCCTTCGGGATGACCAAGGTCTTCATC
GGCGTGATCGTGGTCGCCATCGTCGGCAACGCCGCCGAGCACAGCAGCGCCGTCATGCTCGCGCTGAAGAATCGCATGGA
GCTTGCCATGGGGATCGCGGTCGGCAGCAGCCTGCAGATCGCCCTCTTCGTGGCCCCGCTGCTGGTGATCGTCAGCCATT
TCATCGGTCCCCGGCCGATGGATCTGATGTTCACGCCGGCGGAAGTTCTCGCCATCTTCCTCGCCGTCCTCATCACCGGG
CAGATCTCCTCCGACGGCGAATCCAATTGGATCGAAGGCGTCCAGCTTCTGGCCGTCTACGTCATCATGGCCATCGTCTT
TTTCTTCCTTCCCGAGGCGGGGTAA

Upstream 100 bases:

>100_bases
ATCAGTGGCTCGACTATTACAACGCTCGCAGCTTCCCCATGCAGTTCAACAAAGTCGAGCCCGCGAGCACGCTGCAATTC
CGTCCCACGCGCTGACCCGC

Downstream 100 bases:

>100_bases
CGCTCTTGCTACGCTTATGGAATGCCGTCCAATTCTGAAATCGCGAACACTCTCACTCAATCCCTGCGTCTGGCGCAGCC
GCCCGTGGCTGTCGGTCTGA

Product: calcium/cation antiporter

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLNWLLIFVPVAIGLEFFTPGQHTLIFITACLAVIPLAGWLGHATEELARYTGEGAGGLLNATFGNAAELIIAFMAIRKG
LYSVVKASLTGSIIGNILLVLGAAVLAGGLKHKHQRFNVTGARAQATLLTLAAIALVMPAAFHALAGPPNIVKEDGLSLA
IACVLLIAYGLHLLFSLRTHKQLFEGEPEHQEEPEPAAWSLKRALLTLAGATLVIAWVSEILVGSVEQTAAAFGMTKVFI
GVIVVAIVGNAAEHSSAVMLALKNRMELAMGIAVGSSLQIALFVAPLLVIVSHFIGPRPMDLMFTPAEVLAIFLAVLITG
QISSDGESNWIEGVQLLAVYVIMAIVFFFLPEAG

Sequences:

>Translated_354_residues
MLNWLLIFVPVAIGLEFFTPGQHTLIFITACLAVIPLAGWLGHATEELARYTGEGAGGLLNATFGNAAELIIAFMAIRKG
LYSVVKASLTGSIIGNILLVLGAAVLAGGLKHKHQRFNVTGARAQATLLTLAAIALVMPAAFHALAGPPNIVKEDGLSLA
IACVLLIAYGLHLLFSLRTHKQLFEGEPEHQEEPEPAAWSLKRALLTLAGATLVIAWVSEILVGSVEQTAAAFGMTKVFI
GVIVVAIVGNAAEHSSAVMLALKNRMELAMGIAVGSSLQIALFVAPLLVIVSHFIGPRPMDLMFTPAEVLAIFLAVLITG
QISSDGESNWIEGVQLLAVYVIMAIVFFFLPEAG
>Mature_354_residues
MLNWLLIFVPVAIGLEFFTPGQHTLIFITACLAVIPLAGWLGHATEELARYTGEGAGGLLNATFGNAAELIIAFMAIRKG
LYSVVKASLTGSIIGNILLVLGAAVLAGGLKHKHQRFNVTGARAQATLLTLAAIALVMPAAFHALAGPPNIVKEDGLSLA
IACVLLIAYGLHLLFSLRTHKQLFEGEPEHQEEPEPAAWSLKRALLTLAGATLVIAWVSEILVGSVEQTAAAFGMTKVFI
GVIVVAIVGNAAEHSSAVMLALKNRMELAMGIAVGSSLQIALFVAPLLVIVSHFIGPRPMDLMFTPAEVLAIFLAVLITG
QISSDGESNWIEGVQLLAVYVIMAIVFFFLPEAG

Specific function: According To Ref.1 The Natural Activity Of This Protein Is As A Calcium/Proton Antiporter, But It Can Act As A Weak Sodium/Proton Antiporter. The Presence Of A Preferred Substrate And Normal Ph Cytoplasmic Values Might Prevent Significant Sodium/Proton An

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320075, Length=366, Percent_Identity=43.9890710382514, Blast_Score=262, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6324008, Length=374, Percent_Identity=27.5401069518717, Blast_Score=132, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004798
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 37329; Mature: 37329

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNWLLIFVPVAIGLEFFTPGQHTLIFITACLAVIPLAGWLGHATEELARYTGEGAGGLL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
NATFGNAAELIIAFMAIRKGLYSVVKASLTGSIIGNILLVLGAAVLAGGLKHKHQRFNVT
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GARAQATLLTLAAIALVMPAAFHALAGPPNIVKEDGLSLAIACVLLIAYGLHLLFSLRTH
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KQLFEGEPEHQEEPEPAAWSLKRALLTLAGATLVIAWVSEILVGSVEQTAAAFGMTKVFI
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GVIVVAIVGNAAEHSSAVMLALKNRMELAMGIAVGSSLQIALFVAPLLVIVSHFIGPRPM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
DLMFTPAEVLAIFLAVLITGQISSDGESNWIEGVQLLAVYVIMAIVFFFLPEAG
CEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNWLLIFVPVAIGLEFFTPGQHTLIFITACLAVIPLAGWLGHATEELARYTGEGAGGLL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
NATFGNAAELIIAFMAIRKGLYSVVKASLTGSIIGNILLVLGAAVLAGGLKHKHQRFNVT
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GARAQATLLTLAAIALVMPAAFHALAGPPNIVKEDGLSLAIACVLLIAYGLHLLFSLRTH
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KQLFEGEPEHQEEPEPAAWSLKRALLTLAGATLVIAWVSEILVGSVEQTAAAFGMTKVFI
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GVIVVAIVGNAAEHSSAVMLALKNRMELAMGIAVGSSLQIALFVAPLLVIVSHFIGPRPM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
DLMFTPAEVLAIFLAVLITGQISSDGESNWIEGVQLLAVYVIMAIVFFFLPEAG
CEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]