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The map label for this gene is mltF [H]

Identifier: 116623957

GI number: 116623957

Start: 6148223

End: 6149800

Strand: Reverse

Name: mltF [H]

Synonym: Acid_4869

Alternate gene names: 116623957

Gene position: 6149800-6148223 (Counterclockwise)

Preceding gene: 116623958

Following gene: 116623956

Centisome position: 61.71

GC content: 58.81

Gene sequence:

>1578_bases
ATGTTGTGGCCATCGAGGCATGCCTTCGCCACGTGCGGAGGCGTCATATTACTGGTCATCTTCGCGGCTTGCAGCGGGGG
CGGGAAGCCAGTCACGAGAGCTGAGACCAAGACTGTGGAGCAGGCGCCGCCGGCCCCGGAAACTGCACCGCCGCAAGGCG
TCGCCCACCGCGTGACGGCGGCGGCAAATATCGTAACGCGGGGGCCAATCGAACAGCGCCTGGTACGTGAAAAATGGCAT
GGCGATCTGGATCGAATCGCAAGACGCAGGTTCCTACGGGTGCTGGTTACGCCCAGCAAACTCGGGTTCCATTTCGCCGG
AGGCCAGATGCTGGGCGCAATCCACGAAGTCTGTCGCGAGTTCGAAAAGTTTCTCAGCAAGAAACTGAACACGGGGAATC
TCTCGATCGAGGCAGTGTTCATCCCGGTTAGCCGTGAGACGCTGATCCCGATGCTCGAGGACGGCTCGGGGGATCTGGTC
GCAAGCCTCGTCGGCGCCAGCGAACGGTATCAGGAAAGTGTTGACTACAGCGACCCGGTCTGGGACGGGGCAAAGGCCGT
TATTGTGAGCGGCCCGAGTGCTCCGCCTATCGGCCAGTTGAGCGACCTGGCGGGCCAGGAGATCTATTACTACCGTGACA
CCATCCCCTTCGACAAGTTAGGCCAGCTCAGCGAAAGCTTTAAGGAAAAGGGCAAGCAGCCGATTCAGTTAACCGCAACC
GACAGGGACTTACAGGCGGATGATTTACTGGAAATGGTCAACGCGGGTCTGGTGCCCATGACGGTGGCCGAGGAGCGTGT
GGCCACGGTGTTCGCCCGGATTCTGCCCCACCTCTTGATCAACTCCGGCGTTGTTGTGGCGGAGGGTCCGGTACGCTGGG
CGATCCGGAAGAACACCCCGCAATTGAAGGCCGCGGTAAATGAGTTTATCCAGAGTCATCGGCCCGGAACGGCTTACGGC
AACATGGTAGCGCGCCGATACCTGCGCGACGAAAAGTGGGTGAAAGACGCCACAGCCAGAAAGGACCTTGCCCGGTTCGA
GGAACTCGTTAGTCTCTTCCGGCAGTACGGTGAGAAATACGATTTTCCGTATCTTCTGCTGGCTGCGCAAGCGTACCAGG
AATCGCGCCTGAACCCCAACCTGAGGAGCAGGGCCGGTGCGGTGGGTGTTATGCAAATCAAGCCGAGCACCGCCGCGGCG
AGTCCGATCAATATCTCCGACGTCATTAAGACAGACCGCAACGTGGAATCCGGAGCCAAGTATTTACGATACATGGCCGA
TCAATACTACGGGAACGAACCGATGGACCAGGTTACCAGGGGTGTGTTCGCCATCGCTTCGTACAATGCCGGCCCGGGCA
TGATTCGAAAACTGCGAGCCCAGGCGGCCGACGAGGGGTACGATCCCAATCGGTGGTCCAACAACGTGGAGATCATCGCA
TCAAAGAGAATCGGCCATGAAACGGTTCAGTATGTCGGGAACATATACAAGTATTACCTGGCTTACAAAATGATTACGGA
GAAAGACGCGAAGAGGCTAGCCGCGCGCCAGAGTGCCGCGACCCGCGCCGGTAAGTGA

Upstream 100 bases:

>100_bases
ACTGGCCGGCGTGGTTTATCCCGCCCTGGCGCACAGAGGAGCAACGGAAACCGTACGCACCCCGCGGCTCGCGGCCTGAC
GCACGAGGCAAACAAAAACC

Downstream 100 bases:

>100_bases
AGTCGCAGACAGTACAGTGAGGCCATACCAATGCAGAATCTGTTCACGAAGCTGATCCTCGAACCTGGGATTAAGATCAA
GCTAGATGAAATCTTCCCGG

Product: lytic transglycosylase catalytic subunit subunit

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MLWPSRHAFATCGGVILLVIFAACSGGGKPVTRAETKTVEQAPPAPETAPPQGVAHRVTAAANIVTRGPIEQRLVREKWH
GDLDRIARRRFLRVLVTPSKLGFHFAGGQMLGAIHEVCREFEKFLSKKLNTGNLSIEAVFIPVSRETLIPMLEDGSGDLV
ASLVGASERYQESVDYSDPVWDGAKAVIVSGPSAPPIGQLSDLAGQEIYYYRDTIPFDKLGQLSESFKEKGKQPIQLTAT
DRDLQADDLLEMVNAGLVPMTVAEERVATVFARILPHLLINSGVVVAEGPVRWAIRKNTPQLKAAVNEFIQSHRPGTAYG
NMVARRYLRDEKWVKDATARKDLARFEELVSLFRQYGEKYDFPYLLLAAQAYQESRLNPNLRSRAGAVGVMQIKPSTAAA
SPINISDVIKTDRNVESGAKYLRYMADQYYGNEPMDQVTRGVFAIASYNAGPGMIRKLRAQAADEGYDPNRWSNNVEIIA
SKRIGHETVQYVGNIYKYYLAYKMITEKDAKRLAARQSAATRAGK

Sequences:

>Translated_525_residues
MLWPSRHAFATCGGVILLVIFAACSGGGKPVTRAETKTVEQAPPAPETAPPQGVAHRVTAAANIVTRGPIEQRLVREKWH
GDLDRIARRRFLRVLVTPSKLGFHFAGGQMLGAIHEVCREFEKFLSKKLNTGNLSIEAVFIPVSRETLIPMLEDGSGDLV
ASLVGASERYQESVDYSDPVWDGAKAVIVSGPSAPPIGQLSDLAGQEIYYYRDTIPFDKLGQLSESFKEKGKQPIQLTAT
DRDLQADDLLEMVNAGLVPMTVAEERVATVFARILPHLLINSGVVVAEGPVRWAIRKNTPQLKAAVNEFIQSHRPGTAYG
NMVARRYLRDEKWVKDATARKDLARFEELVSLFRQYGEKYDFPYLLLAAQAYQESRLNPNLRSRAGAVGVMQIKPSTAAA
SPINISDVIKTDRNVESGAKYLRYMADQYYGNEPMDQVTRGVFAIASYNAGPGMIRKLRAQAADEGYDPNRWSNNVEIIA
SKRIGHETVQYVGNIYKYYLAYKMITEKDAKRLAARQSAATRAGK
>Mature_525_residues
MLWPSRHAFATCGGVILLVIFAACSGGGKPVTRAETKTVEQAPPAPETAPPQGVAHRVTAAANIVTRGPIEQRLVREKWH
GDLDRIARRRFLRVLVTPSKLGFHFAGGQMLGAIHEVCREFEKFLSKKLNTGNLSIEAVFIPVSRETLIPMLEDGSGDLV
ASLVGASERYQESVDYSDPVWDGAKAVIVSGPSAPPIGQLSDLAGQEIYYYRDTIPFDKLGQLSESFKEKGKQPIQLTAT
DRDLQADDLLEMVNAGLVPMTVAEERVATVFARILPHLLINSGVVVAEGPVRWAIRKNTPQLKAAVNEFIQSHRPGTAYG
NMVARRYLRDEKWVKDATARKDLARFEELVSLFRQYGEKYDFPYLLLAAQAYQESRLNPNLRSRAGAVGVMQIKPSTAAA
SPINISDVIKTDRNVESGAKYLRYMADQYYGNEPMDQVTRGVFAIASYNAGPGMIRKLRAQAADEGYDPNRWSNNVEIIA
SKRIGHETVQYVGNIYKYYLAYKMITEKDAKRLAARQSAATRAGK

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=452, Percent_Identity=26.3274336283186, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082441, Length=254, Percent_Identity=26.3779527559055, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 58076; Mature: 58076

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWPSRHAFATCGGVILLVIFAACSGGGKPVTRAETKTVEQAPPAPETAPPQGVAHRVTA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHH
AANIVTRGPIEQRLVREKWHGDLDRIARRRFLRVLVTPSKLGFHFAGGQMLGAIHEVCRE
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHH
FEKFLSKKLNTGNLSIEAVFIPVSRETLIPMLEDGSGDLVASLVGASERYQESVDYSDPV
HHHHHHHCCCCCCEEEEEEEEECCHHHHCCCEECCCCHHHHHHHCCHHHHHHHCCCCCCC
WDGAKAVIVSGPSAPPIGQLSDLAGQEIYYYRDTIPFDKLGQLSESFKEKGKQPIQLTAT
CCCCCEEEEECCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEC
DRDLQADDLLEMVNAGLVPMTVAEERVATVFARILPHLLINSGVVVAEGPVRWAIRKNTP
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCH
QLKAAVNEFIQSHRPGTAYGNMVARRYLRDEKWVKDATARKDLARFEELVSLFRQYGEKY
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFPYLLLAAQAYQESRLNPNLRSRAGAVGVMQIKPSTAAASPINISDVIKTDRNVESGAK
CCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCHHHHHH
YLRYMADQYYGNEPMDQVTRGVFAIASYNAGPGMIRKLRAQAADEGYDPNRWSNNVEIIA
HHHHHHHHHCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
SKRIGHETVQYVGNIYKYYLAYKMITEKDAKRLAARQSAATRAGK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLWPSRHAFATCGGVILLVIFAACSGGGKPVTRAETKTVEQAPPAPETAPPQGVAHRVTA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHH
AANIVTRGPIEQRLVREKWHGDLDRIARRRFLRVLVTPSKLGFHFAGGQMLGAIHEVCRE
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHH
FEKFLSKKLNTGNLSIEAVFIPVSRETLIPMLEDGSGDLVASLVGASERYQESVDYSDPV
HHHHHHHCCCCCCEEEEEEEEECCHHHHCCCEECCCCHHHHHHHCCHHHHHHHCCCCCCC
WDGAKAVIVSGPSAPPIGQLSDLAGQEIYYYRDTIPFDKLGQLSESFKEKGKQPIQLTAT
CCCCCEEEEECCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEC
DRDLQADDLLEMVNAGLVPMTVAEERVATVFARILPHLLINSGVVVAEGPVRWAIRKNTP
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCH
QLKAAVNEFIQSHRPGTAYGNMVARRYLRDEKWVKDATARKDLARFEELVSLFRQYGEKY
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DFPYLLLAAQAYQESRLNPNLRSRAGAVGVMQIKPSTAAASPINISDVIKTDRNVESGAK
CCCHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCHHHHHH
YLRYMADQYYGNEPMDQVTRGVFAIASYNAGPGMIRKLRAQAADEGYDPNRWSNNVEIIA
HHHHHHHHHCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
SKRIGHETVQYVGNIYKYYLAYKMITEKDAKRLAARQSAATRAGK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA