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The map label for this gene is epsC [H]

Identifier: 116623949

GI number: 116623949

Start: 6138726

End: 6140639

Strand: Direct

Name: epsC [H]

Synonym: Acid_4861

Alternate gene names: 116623949

Gene position: 6138726-6140639 (Clockwise)

Preceding gene: 116623948

Following gene: 116623950

Centisome position: 61.6

GC content: 61.29

Gene sequence:

>1914_bases
ATGAAAAACCATCGGAATATCGATGTCTGGCTGGAGCGTGCGTGCCATCTCACGGTGGTCGGGCTATCACTGACGGCGGC
ATTCCTGCTTCGGTTCGACTTTGCGATTCCAGCCAGCGTGGTACCAATCTGGAAGCAAGCGCTGCTGATCGCGATCCTGG
TGAAATTGCCGATCTTTGATTGGGTCGGGTTCTATCGCAGCTTGCGGCGATTCGTCAGCATCCCTGACCTCTACCTGGTG
TTCCTGGGAAATGTGGCGGGCAGTTTGCTGCTGGCGGCGATGTCGATGTTCTTCACCGGGCCGGCGATGCCCCGCTCCGT
GCTGATCATGGACGGCATTCTGTGCTTTGTGGCGACGGCGCTGGTACGGTTTTCGGTTCGCATCTACAACGCTACCTTCC
GGGAGCGTTCCGCCGAGCAGCGGACCGGAATCCTCATCTACGGCGCGGGCGCGGCGGGTGCGGAGTTGGCGCGGGAAATC
CATGCCAACCGCAACACGAAGTACGAAGTGAAAGGGTTTTTGGACGACGATCCGCTGAAACAGAATGCGCGGATCATGGG
TATACCGGTTTTGGGACAGGGCCGCGAAGCCTATTCCGTAGTGCGACAACGGAACCGACGCGGACCCAGCGTCGGCGAGA
TCATCATCGCGATGCCCTCGGCCACCGGACAGCAGATGCGCGAGACGCTGGCCAACTGCCGCGCCGCGGGCGTCCCGTGC
AAGACTATCCCGGGAATCGACGAACTCCTGAGTGGGAGAGTCTTGCTGGCGCAGGTGCGCAATCCATCCGTGCAGGATTT
ACTGGGGCGGCAGCAGGTGCGGCTGGACGAGGCTCACGTGGCGGCCAGTATCGCCGGCCGTTCCGTCCTGGTGACGGGCG
CCGCGGGCTCGATCGGTTCGGAGTTGTGCCGGCAGGTGGCGCGCTTCGGACCGGGGTGCCTTATCGCATTCGACCAGGCC
GAGAGCGACCTCTTCCGCATCGAGAACGAACTTCGGGAACGCTACCCGGAACTGGAACTGGTAACCGCGCTGGGCAATAT
CCGTGAAGCCGAGCGGCTGTCTGAGGTGATGCGGCAGCATGCGGTGGAATCGATTTTCCATGCGGCGGCTTACAAGCATG
TCCCGATGATGGAATCGCACCCGCTCGAGGCGGTCTGCAACAACGTGCTTGGAACGTGGAACCTGGTGCGCGCGGCACGC
GGACACAACGTACGCAACCTGCTGATGATCTCGTCGGACAAGGCGGTGAACCCGAGTTGCGTGATGGGCGCGACCAAGCG
CGTCTGCGAGCGGATCGTCTCCTCGCGCTGGCCGGGGAACGGGCAGACCAAGTGTGTTTCGGTCCGATTTGGAAACGTCC
TCGGCAGCAATGGCAGCGTGGTCCCGGTGTTTCAGGCCCAGATTGCCGCGGGTGGGCCGATCAAAGTGACGCACCCGGAT
GCGCGCCGCTTCTTCATGACCATCTCCGAGGCCGTGTCGCTGGTGATTCAGGCTTCGACGAGAACCGAAGGCTCGGAGAT
TTTCGTTCTGGATATGGGCGAACCGATCCGCATCGTCGATCTGGCCGAGAACATGATCCGGCTGGCCGGCAAGGTGCCCT
ATGAAGATATCGACATCCAATTCACGGGAATGCGGCCGGGCGAAAAATTGATGGAGGAAATCAGGGGCAAGAGTGAGCGG
CTGATTCCCACTTCCATGGAAAAGATGCACGCCATTCAGGAGCAGCCCCTTGTGTGGGACAAGATCAACGGGTGGATCGA
GGAGTTGAAGGAACTGATCGCGGAGAGGCAGGAGCGCCGGATCATCGCGCATCTGCAAACACTGGTGCCGGAGTATACCC
CGTCTATGCGAGGGGCGCCCCAGCCGGTCATCATGCTGCCGGTTAAGAGACCGAGCGAATTGCGTTCGGCGTAG

Upstream 100 bases:

>100_bases
TCGAAAGTTCCAGACGAGGAAGGTTAGTGGCGGCAGGCACACCGGCCGCGATGGTCCCGGCCACTGTCTCGGACATTGCC
GGCTCTTAGGAGGGATGCAT

Downstream 100 bases:

>100_bases
TGAAGAGGTTGAACGAGCGGAAGGCTGGATCGAATTCCTATGTTGCCTAGCGCCTTAGTTGAAGCACAACTCTCAAGAGA
GGACGCCGCCGATGCGCGGT

Product: polysaccharide biosynthesis protein CapD

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MKNHRNIDVWLERACHLTVVGLSLTAAFLLRFDFAIPASVVPIWKQALLIAILVKLPIFDWVGFYRSLRRFVSIPDLYLV
FLGNVAGSLLLAAMSMFFTGPAMPRSVLIMDGILCFVATALVRFSVRIYNATFRERSAEQRTGILIYGAGAAGAELAREI
HANRNTKYEVKGFLDDDPLKQNARIMGIPVLGQGREAYSVVRQRNRRGPSVGEIIIAMPSATGQQMRETLANCRAAGVPC
KTIPGIDELLSGRVLLAQVRNPSVQDLLGRQQVRLDEAHVAASIAGRSVLVTGAAGSIGSELCRQVARFGPGCLIAFDQA
ESDLFRIENELRERYPELELVTALGNIREAERLSEVMRQHAVESIFHAAAYKHVPMMESHPLEAVCNNVLGTWNLVRAAR
GHNVRNLLMISSDKAVNPSCVMGATKRVCERIVSSRWPGNGQTKCVSVRFGNVLGSNGSVVPVFQAQIAAGGPIKVTHPD
ARRFFMTISEAVSLVIQASTRTEGSEIFVLDMGEPIRIVDLAENMIRLAGKVPYEDIDIQFTGMRPGEKLMEEIRGKSER
LIPTSMEKMHAIQEQPLVWDKINGWIEELKELIAERQERRIIAHLQTLVPEYTPSMRGAPQPVIMLPVKRPSELRSA

Sequences:

>Translated_637_residues
MKNHRNIDVWLERACHLTVVGLSLTAAFLLRFDFAIPASVVPIWKQALLIAILVKLPIFDWVGFYRSLRRFVSIPDLYLV
FLGNVAGSLLLAAMSMFFTGPAMPRSVLIMDGILCFVATALVRFSVRIYNATFRERSAEQRTGILIYGAGAAGAELAREI
HANRNTKYEVKGFLDDDPLKQNARIMGIPVLGQGREAYSVVRQRNRRGPSVGEIIIAMPSATGQQMRETLANCRAAGVPC
KTIPGIDELLSGRVLLAQVRNPSVQDLLGRQQVRLDEAHVAASIAGRSVLVTGAAGSIGSELCRQVARFGPGCLIAFDQA
ESDLFRIENELRERYPELELVTALGNIREAERLSEVMRQHAVESIFHAAAYKHVPMMESHPLEAVCNNVLGTWNLVRAAR
GHNVRNLLMISSDKAVNPSCVMGATKRVCERIVSSRWPGNGQTKCVSVRFGNVLGSNGSVVPVFQAQIAAGGPIKVTHPD
ARRFFMTISEAVSLVIQASTRTEGSEIFVLDMGEPIRIVDLAENMIRLAGKVPYEDIDIQFTGMRPGEKLMEEIRGKSER
LIPTSMEKMHAIQEQPLVWDKINGWIEELKELIAERQERRIIAHLQTLVPEYTPSMRGAPQPVIMLPVKRPSELRSA
>Mature_637_residues
MKNHRNIDVWLERACHLTVVGLSLTAAFLLRFDFAIPASVVPIWKQALLIAILVKLPIFDWVGFYRSLRRFVSIPDLYLV
FLGNVAGSLLLAAMSMFFTGPAMPRSVLIMDGILCFVATALVRFSVRIYNATFRERSAEQRTGILIYGAGAAGAELAREI
HANRNTKYEVKGFLDDDPLKQNARIMGIPVLGQGREAYSVVRQRNRRGPSVGEIIIAMPSATGQQMRETLANCRAAGVPC
KTIPGIDELLSGRVLLAQVRNPSVQDLLGRQQVRLDEAHVAASIAGRSVLVTGAAGSIGSELCRQVARFGPGCLIAFDQA
ESDLFRIENELRERYPELELVTALGNIREAERLSEVMRQHAVESIFHAAAYKHVPMMESHPLEAVCNNVLGTWNLVRAAR
GHNVRNLLMISSDKAVNPSCVMGATKRVCERIVSSRWPGNGQTKCVSVRFGNVLGSNGSVVPVFQAQIAAGGPIKVTHPD
ARRFFMTISEAVSLVIQASTRTEGSEIFVLDMGEPIRIVDLAENMIRLAGKVPYEDIDIQFTGMRPGEKLMEEIRGKSER
LIPTSMEKMHAIQEQPLVWDKINGWIEELKELIAERQERRIIAHLQTLVPEYTPSMRGAPQPVIMLPVKRPSELRSA

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786974, Length=188, Percent_Identity=27.1276595744681, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 70497; Mature: 70497

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNHRNIDVWLERACHLTVVGLSLTAAFLLRFDFAIPASVVPIWKQALLIAILVKLPIFD
CCCCCCHHHHHHCCHHEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
WVGFYRSLRRFVSIPDLYLVFLGNVAGSLLLAAMSMFFTGPAMPRSVLIMDGILCFVATA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LVRFSVRIYNATFRERSAEQRTGILIYGAGAAGAELAREIHANRNTKYEVKGFLDDDPLK
HHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCC
QNARIMGIPVLGQGREAYSVVRQRNRRGPSVGEIIIAMPSATGQQMRETLANCRAAGVPC
CCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
KTIPGIDELLSGRVLLAQVRNPSVQDLLGRQQVRLDEAHVAASIAGRSVLVTGAAGSIGS
CCCCCHHHHHCCCEEEEECCCCCHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
ELCRQVARFGPGCLIAFDQAESDLFRIENELRERYPELELVTALGNIREAERLSEVMRQH
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AVESIFHAAAYKHVPMMESHPLEAVCNNVLGTWNLVRAARGHNVRNLLMISSDKAVNPSC
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHH
VMGATKRVCERIVSSRWPGNGQTKCVSVRFGNVLGSNGSVVPVFQAQIAAGGPIKVTHPD
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEECCCCCEEEEEEHEECCCCCEEEECCH
ARRFFMTISEAVSLVIQASTRTEGSEIFVLDMGEPIRIVDLAENMIRLAGKVPYEDIDIQ
HHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCEE
FTGMRPGEKLMEEIRGKSERLIPTSMEKMHAIQEQPLVWDKINGWIEELKELIAERQERR
EECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
IIAHLQTLVPEYTPSMRGAPQPVIMLPVKRPSELRSA
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHCCC
>Mature Secondary Structure
MKNHRNIDVWLERACHLTVVGLSLTAAFLLRFDFAIPASVVPIWKQALLIAILVKLPIFD
CCCCCCHHHHHHCCHHEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
WVGFYRSLRRFVSIPDLYLVFLGNVAGSLLLAAMSMFFTGPAMPRSVLIMDGILCFVATA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LVRFSVRIYNATFRERSAEQRTGILIYGAGAAGAELAREIHANRNTKYEVKGFLDDDPLK
HHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCC
QNARIMGIPVLGQGREAYSVVRQRNRRGPSVGEIIIAMPSATGQQMRETLANCRAAGVPC
CCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
KTIPGIDELLSGRVLLAQVRNPSVQDLLGRQQVRLDEAHVAASIAGRSVLVTGAAGSIGS
CCCCCHHHHHCCCEEEEECCCCCHHHHHCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
ELCRQVARFGPGCLIAFDQAESDLFRIENELRERYPELELVTALGNIREAERLSEVMRQH
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AVESIFHAAAYKHVPMMESHPLEAVCNNVLGTWNLVRAARGHNVRNLLMISSDKAVNPSC
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHH
VMGATKRVCERIVSSRWPGNGQTKCVSVRFGNVLGSNGSVVPVFQAQIAAGGPIKVTHPD
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEECCCCCEEEEEEHEECCCCCEEEECCH
ARRFFMTISEAVSLVIQASTRTEGSEIFVLDMGEPIRIVDLAENMIRLAGKVPYEDIDIQ
HHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCEE
FTGMRPGEKLMEEIRGKSERLIPTSMEKMHAIQEQPLVWDKINGWIEELKELIAERQERR
EECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
IIAHLQTLVPEYTPSMRGAPQPVIMLPVKRPSELRSA
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]