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The map label for this gene is greB [H]

Identifier: 116623798

GI number: 116623798

Start: 5962447

End: 5963130

Strand: Reverse

Name: greB [H]

Synonym: Acid_4710

Alternate gene names: 116623798

Gene position: 5963130-5962447 (Counterclockwise)

Preceding gene: 116623799

Following gene: 116623797

Centisome position: 59.84

GC content: 62.43

Gene sequence:

>684_bases
GTGAACGCTGTGCGGCTTTGCGCGAATATAAAGACGCGAGGATCACAGCAAGTTTGCGTGTGGTCAACGGGCTCCGATAT
GCGAAAAGGGTTTACGAGAAAACCGCAAGACGAGCCCGGGACGCCAGGCAAGAACTATATAACCCCGGATGGGCTGCAGC
GCCTCAAAGAGGAGCACCGGTTTCTGCTGACCAGGGAACGCCCGGCCGTGACCAAGGTGGTGGCATGGGCTGCGAGCAAC
GGCGATCGCAGTGAAAACGCCGACTATCAGTACGGCAAGCGGCGGCTGCGCCAGATCGACGGGCGGATTCGCTTTCTGAC
GAAGCGAATCGATGCCGCGGAAGTGGTGGACCCGGAACGTCCGAGGGCGGGGCAGGCGGCGACGCGGGCATTCTTCGGAG
CGACGGTGCATTATGCCAATGCCGCAGGAACGGAGCGAGTGGTGAGCATCGTGGGCACCGACGAGGTCGATCTCAATCGC
AACCACATCAGTTGGGTGTCGCCTCTGGGGCGCGCGTTGATGAAGTCGGCAGCGGGGGACCGCGTAGTTCTACAAGCGCC
GGGCGGTACAGAACACCTGGTCGTCCTGAAGGTGTGTTACGAGCGCATTTCGGTTGAACCGTTCCGTGAGCCGCCTGGAG
CGGAGGCCCCGGTAGAGGGGCTCCCTCGCCGAAGCGGCACATGA

Upstream 100 bases:

>100_bases
TCGTAGTCCCCGAGAAGGTCTAAAGAAATATTTCGCGCAAAGGCGCAAAGAACGCAAAGAAGAACCGAGCGGAGAGCCCC
AAAAAGGGGCCCGGCATTCG

Downstream 100 bases:

>100_bases
ATGTCCGACCCGAGCCAGGGTCGCTCACGTGCAACGTCGCAGTAGCGATTGACCCATCAGATCCGGCGGCATGGTAAGCT
CATCCGAAAAGGAGGGCACA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MNAVRLCANIKTRGSQQVCVWSTGSDMRKGFTRKPQDEPGTPGKNYITPDGLQRLKEEHRFLLTRERPAVTKVVAWAASN
GDRSENADYQYGKRRLRQIDGRIRFLTKRIDAAEVVDPERPRAGQAATRAFFGATVHYANAAGTERVVSIVGTDEVDLNR
NHISWVSPLGRALMKSAAGDRVVLQAPGGTEHLVVLKVCYERISVEPFREPPGAEAPVEGLPRRSGT

Sequences:

>Translated_227_residues
MNAVRLCANIKTRGSQQVCVWSTGSDMRKGFTRKPQDEPGTPGKNYITPDGLQRLKEEHRFLLTRERPAVTKVVAWAASN
GDRSENADYQYGKRRLRQIDGRIRFLTKRIDAAEVVDPERPRAGQAATRAFFGATVHYANAAGTERVVSIVGTDEVDLNR
NHISWVSPLGRALMKSAAGDRVVLQAPGGTEHLVVLKVCYERISVEPFREPPGAEAPVEGLPRRSGT
>Mature_227_residues
MNAVRLCANIKTRGSQQVCVWSTGSDMRKGFTRKPQDEPGTPGKNYITPDGLQRLKEEHRFLLTRERPAVTKVVAWAASN
GDRSENADYQYGKRRLRQIDGRIRFLTKRIDAAEVVDPERPRAGQAATRAFFGATVHYANAAGTERVVSIVGTDEVDLNR
NHISWVSPLGRALMKSAAGDRVVLQAPGGTEHLVVLKVCYERISVEPFREPPGAEAPVEGLPRRSGT

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=44.8051948051948, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI87082225, Length=156, Percent_Identity=35.8974358974359, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 25091; Mature: 25091

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVRLCANIKTRGSQQVCVWSTGSDMRKGFTRKPQDEPGTPGKNYITPDGLQRLKEEHR
CCHHEEEHHHCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FLLTRERPAVTKVVAWAASNGDRSENADYQYGKRRLRQIDGRIRFLTKRIDAAEVVDPER
EEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
PRAGQAATRAFFGATVHYANAAGTERVVSIVGTDEVDLNRNHISWVSPLGRALMKSAAGD
CCCCHHHHHHHHHHHEEECCCCCHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
RVVLQAPGGTEHLVVLKVCYERISVEPFREPPGAEAPVEGLPRRSGT
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAVRLCANIKTRGSQQVCVWSTGSDMRKGFTRKPQDEPGTPGKNYITPDGLQRLKEEHR
CCHHEEEHHHCCCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FLLTRERPAVTKVVAWAASNGDRSENADYQYGKRRLRQIDGRIRFLTKRIDAAEVVDPER
EEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
PRAGQAATRAFFGATVHYANAAGTERVVSIVGTDEVDLNRNHISWVSPLGRALMKSAAGD
CCCCHHHHHHHHHHHEEECCCCCHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
RVVLQAPGGTEHLVVLKVCYERISVEPFREPPGAEAPVEGLPRRSGT
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]