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The map label for this gene is wcaF [H]

Identifier: 116623744

GI number: 116623744

Start: 5887260

End: 5887844

Strand: Direct

Name: wcaF [H]

Synonym: Acid_4656

Alternate gene names: 116623744

Gene position: 5887260-5887844 (Clockwise)

Preceding gene: 116623743

Following gene: 116623746

Centisome position: 59.08

GC content: 56.75

Gene sequence:

>585_bases
ATGGCAATTGACCTTTCGAAATTCACGAATTCATGGTATTCGCCCGGGCGCTCGCGGAATTTTCAAGCTCTCTGGTACGC
GGTAGGGCTGCCAATCTTGCGGTCATGTTGGGTCCCGTCGTACAGCGTCCGACGGCAAGTTCTGCGTCTATTTGGAGCTA
AGATCGGATGCGGTGTGGTGATCAAGCCCGGCGTCAGGGTCAAGTATCCGTGGTTTCTGGAAATCGGCGACCATTCCTGG
ATCGGGGAAGACGTCTGGATCGACAACTTGGCGCTCGTCCAGATCGGAGCCAACGTGTGCCTCTCGCAGGGCGCATATCT
ATGCACCGGTAACCACGATTGGAGCGACCCTGCATTTGGACTTATCGTACAACCGATTGTTATCCATGCCGGCGCGTGGG
TAGGTGCAAAGACAATAGTCGGACCCGGTGCCGTGATCGGCTTGCAAGCGATCGCTACCGCGGGCAGCGTGGTCACCGGG
AATCTTGACCCTGACACCATTTACTCCGGCAATCCGGCTGTTGCCAGGAAAGCGAGAGTCATCCGAACCGAAGAGGCTCG
CTACTTTACCCCAGCTGCGGAATAG

Upstream 100 bases:

>100_bases
GTGTTATCGCGTTGGCGCCGTTCCGCCGACCCCGTGCTGAACTTGAGATTAGCTCTGCAGAATGCGCGACTGCTTCCCGT
CACTGATTCAAAAAGCGTCC

Downstream 100 bases:

>100_bases
ATTGTCTGGACAACGGCAAGATCCTTGTTGGGAAACCATCCGCGGAACATCAGGATGTCGCCCTCCGCGACAGCGTTCGT
GAGAAATGTCTGATTGATCG

Product: putative colanic acid biosynthesis acetyltransferase WcaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MAIDLSKFTNSWYSPGRSRNFQALWYAVGLPILRSCWVPSYSVRRQVLRLFGAKIGCGVVIKPGVRVKYPWFLEIGDHSW
IGEDVWIDNLALVQIGANVCLSQGAYLCTGNHDWSDPAFGLIVQPIVIHAGAWVGAKTIVGPGAVIGLQAIATAGSVVTG
NLDPDTIYSGNPAVARKARVIRTEEARYFTPAAE

Sequences:

>Translated_194_residues
MAIDLSKFTNSWYSPGRSRNFQALWYAVGLPILRSCWVPSYSVRRQVLRLFGAKIGCGVVIKPGVRVKYPWFLEIGDHSW
IGEDVWIDNLALVQIGANVCLSQGAYLCTGNHDWSDPAFGLIVQPIVIHAGAWVGAKTIVGPGAVIGLQAIATAGSVVTG
NLDPDTIYSGNPAVARKARVIRTEEARYFTPAAE
>Mature_193_residues
AIDLSKFTNSWYSPGRSRNFQALWYAVGLPILRSCWVPSYSVRRQVLRLFGAKIGCGVVIKPGVRVKYPWFLEIGDHSWI
GEDVWIDNLALVQIGANVCLSQGAYLCTGNHDWSDPAFGLIVQPIVIHAGAWVGAKTIVGPGAVIGLQAIATAGSVVTGN
LDPDTIYSGNPAVARKARVIRTEEARYFTPAAE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=159, Percent_Identity=42.7672955974843, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1786664, Length=139, Percent_Identity=26.6187050359712, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 21042; Mature: 20911

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDLSKFTNSWYSPGRSRNFQALWYAVGLPILRSCWVPSYSVRRQVLRLFGAKIGCGVV
CEEEHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEE
IKPGVRVKYPWFLEIGDHSWIGEDVWIDNLALVQIGANVCLSQGAYLCTGNHDWSDPAFG
ECCCCEEECCEEEEECCCCCCCCCEEECCEEEEEECCHHHHCCCCEEEECCCCCCCCCHH
LIVQPIVIHAGAWVGAKTIVGPGAVIGLQAIATAGSVVTGNLDPDTIYSGNPAVARKARV
HHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHCCCCEEECCCCCCCEECCCCHHHHHHHE
IRTEEARYFTPAAE
EEECCCEEECCCCC
>Mature Secondary Structure 
AIDLSKFTNSWYSPGRSRNFQALWYAVGLPILRSCWVPSYSVRRQVLRLFGAKIGCGVV
EEEHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEE
IKPGVRVKYPWFLEIGDHSWIGEDVWIDNLALVQIGANVCLSQGAYLCTGNHDWSDPAFG
ECCCCEEECCEEEEECCCCCCCCCEEECCEEEEEECCHHHHCCCCEEEECCCCCCCCCHH
LIVQPIVIHAGAWVGAKTIVGPGAVIGLQAIATAGSVVTGNLDPDTIYSGNPAVARKARV
HHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHCCCCEEECCCCCCCEECCCCHHHHHHHE
IRTEEARYFTPAAE
EEECCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]