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The map label for this gene is malZ [C]

Identifier: 116623597

GI number: 116623597

Start: 5704182

End: 5705672

Strand: Reverse

Name: malZ [C]

Synonym: Acid_4507

Alternate gene names: 116623597

Gene position: 5705672-5704182 (Counterclockwise)

Preceding gene: 116623598

Following gene: 116623595

Centisome position: 57.25

GC content: 62.51

Gene sequence:

>1491_bases
ATGTCGAAATGGCCCCGGCATCCCACCGTCTTTGAGATCAACACGTGGGTATGGCTTTCCGAACTCAGCCGCAAGGATCA
CGCCTCCCGAAACCTGGCGAACGTGCCCGCGCGGGAATGGGATGAGATCGCCAAATTCGGGTTCGATGCCGTGTGGTTGA
TGGGAGTCTGGGAGCGCAGCCCGGCCGGGATTTCGATCGCCAACCGGAATCCTGGCCTGCTGGAGGATTTCCGGCGGGCC
CTTCCCGATTTTCAACCGGCGGACAACGTGGGTTCTCCCTACTGCGTACGCCGCTACCAGGTGGACCCGCACCTGGGAGG
GCGCGGCGGTCTGGAAACGGCCCGGAAGGAACTCGCGAAACGCGGCATGCGCCTGCTGCTCGATTTCGTTCCGAACCACG
TGGCGCCGGATCATCCCTGGGTGTCGGAGCACCGCGAATACTTCATTCTTGGAAGCGCGGAGGATGCCCGCAATAATCCC
GAATCCTTCGCAGAGGTCGGAGCAGTGATCTATTCTTGCGGTCGCGACCCCTACTTCCCGGCCTGGCCGGACGTGCTGCA
GCTGAATGCTTTTCAGCCAGGTCTCCGTCAGGCGGTTCGTGAGACCCTCTCGGACATCGCGAGTCAGTGCGACGGAATCC
GCTGCGATATGGCAATGCTCCTGTTGAACGCGGTCTTCGCGCGCACCTGGGGCAGCCGTGCGGGTCAACCGCCCGCCACC
GAATACTGGGCTGACCTGATCCCGGCCGTCAAGGCGGCACATCCGGACTTCCTCTTCATCGCAGAAGCCTATTGGGATCT
CGAGTGGGAACTGCAGCAAGAGGGCTTTGACTTCTGTTACGACAAGCGGCTCTACGACCGCCTGGAGCACGATTCCGCGG
AGAGCGTCCGGCTGCATCTCTGCGCCGATCTGCCGTACCAGGAGAGGCTGGTCCGCTTCATTGAGAACCATGACGAGCCT
CGCGCGGCGGCGGCATTCTCTCCGGCAAAGGAGCGTGCCGCCGCGGTGACCATGGCCACCTTGCCGGGAGCCAGCCTCTT
TCATGAGGGGCAGTTTGAGGGACGAAAGGTAAGACTCCCGGTCTTTTTGGGGCGGCTTCCCGATGAACCTATGGACGTGG
AACTGAGCGCGTTTTACCGGAAGCTCCTGGAGACCCTCCGCAGCCCGATCCTTCGCGATGGTCAGTGGAGCCTGTGCAAC
TGCACGGGCTGGCCCGACAATCCAAGCTTTCGGAATCTCGTGGCCTGGAGCTGGGTGAACAACGACGACCGGTTGCTGAT
AGTGGTCAATCTGAGCGGCGATGCCGCGCAAGCCAGGGTGCACCCGCCCTGGAACGACGCACGGGGCCACACTTGGCGGT
TGACCGACAGCCTTTCGGGCGTCTCTTACGATGGCGGAGATGAGATCCAGGCGGACGGGCTGTACGTGGAATTAGAGCCC
TGGGGCCGGCACCTCCTCCGGTGCCAAGCACCGCAATTGATTCACGCATAG

Upstream 100 bases:

>100_bases
GTCGCAAAACTGATCGAACTCTTTGGCAGGCTCGACGGAGCTCAGCTCCTCGCCGCTGGCAAAAGCGGCGCATTCACCAT
CAGGGAAATGAAGGAGCAGT

Downstream 100 bases:

>100_bases
ACCCGGTCGCGCAACTCGATCCGTCCCCGGAGTGGCAGCGTTCGAAGTCTGTACTTTGTGCCTGATAGGCGTTGTCGGTC
CATACGGCGATAGCGGAAAC

Product: alpha amylase catalytic subunit

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: Alpha Amylase; Alpha Amylase Domain-Containing Protein; Alpha-Amylase Family Protein; Alpha Amylase Catalytic Subunit; Alpha-Amylase; Glycosidase; Alpha Amylase Catalytic Domain-Containing Protein; Alpha Amylase Family Protein; Alpha Amylase Domain Protein; Alpha-Glucosidase; Glycoside Hydrolase

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSKWPRHPTVFEINTWVWLSELSRKDHASRNLANVPAREWDEIAKFGFDAVWLMGVWERSPAGISIANRNPGLLEDFRRA
LPDFQPADNVGSPYCVRRYQVDPHLGGRGGLETARKELAKRGMRLLLDFVPNHVAPDHPWVSEHREYFILGSAEDARNNP
ESFAEVGAVIYSCGRDPYFPAWPDVLQLNAFQPGLRQAVRETLSDIASQCDGIRCDMAMLLLNAVFARTWGSRAGQPPAT
EYWADLIPAVKAAHPDFLFIAEAYWDLEWELQQEGFDFCYDKRLYDRLEHDSAESVRLHLCADLPYQERLVRFIENHDEP
RAAAAFSPAKERAAAVTMATLPGASLFHEGQFEGRKVRLPVFLGRLPDEPMDVELSAFYRKLLETLRSPILRDGQWSLCN
CTGWPDNPSFRNLVAWSWVNNDDRLLIVVNLSGDAAQARVHPPWNDARGHTWRLTDSLSGVSYDGGDEIQADGLYVELEP
WGRHLLRCQAPQLIHA

Sequences:

>Translated_496_residues
MSKWPRHPTVFEINTWVWLSELSRKDHASRNLANVPAREWDEIAKFGFDAVWLMGVWERSPAGISIANRNPGLLEDFRRA
LPDFQPADNVGSPYCVRRYQVDPHLGGRGGLETARKELAKRGMRLLLDFVPNHVAPDHPWVSEHREYFILGSAEDARNNP
ESFAEVGAVIYSCGRDPYFPAWPDVLQLNAFQPGLRQAVRETLSDIASQCDGIRCDMAMLLLNAVFARTWGSRAGQPPAT
EYWADLIPAVKAAHPDFLFIAEAYWDLEWELQQEGFDFCYDKRLYDRLEHDSAESVRLHLCADLPYQERLVRFIENHDEP
RAAAAFSPAKERAAAVTMATLPGASLFHEGQFEGRKVRLPVFLGRLPDEPMDVELSAFYRKLLETLRSPILRDGQWSLCN
CTGWPDNPSFRNLVAWSWVNNDDRLLIVVNLSGDAAQARVHPPWNDARGHTWRLTDSLSGVSYDGGDEIQADGLYVELEP
WGRHLLRCQAPQLIHA
>Mature_495_residues
SKWPRHPTVFEINTWVWLSELSRKDHASRNLANVPAREWDEIAKFGFDAVWLMGVWERSPAGISIANRNPGLLEDFRRAL
PDFQPADNVGSPYCVRRYQVDPHLGGRGGLETARKELAKRGMRLLLDFVPNHVAPDHPWVSEHREYFILGSAEDARNNPE
SFAEVGAVIYSCGRDPYFPAWPDVLQLNAFQPGLRQAVRETLSDIASQCDGIRCDMAMLLLNAVFARTWGSRAGQPPATE
YWADLIPAVKAAHPDFLFIAEAYWDLEWELQQEGFDFCYDKRLYDRLEHDSAESVRLHLCADLPYQERLVRFIENHDEPR
AAAAFSPAKERAAAVTMATLPGASLFHEGQFEGRKVRLPVFLGRLPDEPMDVELSAFYRKLLETLRSPILRDGQWSLCNC
TGWPDNPSFRNLVAWSWVNNDDRLLIVVNLSGDAAQARVHPPWNDARGHTWRLTDSLSGVSYDGGDEIQADGLYVELEPW
GRHLLRCQAPQLIHA

Specific function: May Play A Role In Regulating The Intracellular Level Of Maltotriose. Cleaves Glucose From The Reducing End Of Maltotriose And Longer Maltodextrins With A Chain Length Of Up To 7 Glucose Units. [C]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 56380; Mature: 56249

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKWPRHPTVFEINTWVWLSELSRKDHASRNLANVPAREWDEIAKFGFDAVWLMGVWERS
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
PAGISIANRNPGLLEDFRRALPDFQPADNVGSPYCVRRYQVDPHLGGRGGLETARKELAK
CCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
RGMRLLLDFVPNHVAPDHPWVSEHREYFILGSAEDARNNPESFAEVGAVIYSCGRDPYFP
HHHHHHHHHHCCCCCCCCCCHHCCCCEEEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCC
AWPDVLQLNAFQPGLRQAVRETLSDIASQCDGIRCDMAMLLLNAVFARTWGSRAGQPPAT
CCCHHHEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
EYWADLIPAVKAAHPDFLFIAEAYWDLEWELQQEGFDFCYDKRLYDRLEHDSAESVRLHL
HHHHHHHHHHHHCCCCEEEEEEHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEE
CADLPYQERLVRFIENHDEPRAAAAFSPAKERAAAVTMATLPGASLFHEGQFEGRKVRLP
EECCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHHEEEEEECCCHHHHCCCCCCCCEEEEE
VFLGRLPDEPMDVELSAFYRKLLETLRSPILRDGQWSLCNCTGWPDNPSFRNLVAWSWVN
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEEC
NDDRLLIVVNLSGDAAQARVHPPWNDARGHTWRLTDSLSGVSYDGGDEIQADGLYVELEP
CCCCEEEEEECCCCCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCEEECCEEEEECH
WGRHLLRCQAPQLIHA
HHHHHHHCCCCHHHCC
>Mature Secondary Structure 
SKWPRHPTVFEINTWVWLSELSRKDHASRNLANVPAREWDEIAKFGFDAVWLMGVWERS
CCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCC
PAGISIANRNPGLLEDFRRALPDFQPADNVGSPYCVRRYQVDPHLGGRGGLETARKELAK
CCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
RGMRLLLDFVPNHVAPDHPWVSEHREYFILGSAEDARNNPESFAEVGAVIYSCGRDPYFP
HHHHHHHHHHCCCCCCCCCCHHCCCCEEEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCC
AWPDVLQLNAFQPGLRQAVRETLSDIASQCDGIRCDMAMLLLNAVFARTWGSRAGQPPAT
CCCHHHEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHH
EYWADLIPAVKAAHPDFLFIAEAYWDLEWELQQEGFDFCYDKRLYDRLEHDSAESVRLHL
HHHHHHHHHHHHCCCCEEEEEEHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEE
CADLPYQERLVRFIENHDEPRAAAAFSPAKERAAAVTMATLPGASLFHEGQFEGRKVRLP
EECCCHHHHHHHHHHCCCCCCHHHHCCCHHHHHHEEEEEECCCHHHHCCCCCCCCEEEEE
VFLGRLPDEPMDVELSAFYRKLLETLRSPILRDGQWSLCNCTGWPDNPSFRNLVAWSWVN
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEEC
NDDRLLIVVNLSGDAAQARVHPPWNDARGHTWRLTDSLSGVSYDGGDEIQADGLYVELEP
CCCCEEEEEECCCCCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCEEECCEEEEECH
WGRHLLRCQAPQLIHA
HHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA