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The map label for this gene is gatA2 [H]

Identifier: 116623437

GI number: 116623437

Start: 5507724

End: 5509268

Strand: Direct

Name: gatA2 [H]

Synonym: Acid_4346

Alternate gene names: 116623437

Gene position: 5507724-5509268 (Clockwise)

Preceding gene: 116623433

Following gene: 116623438

Centisome position: 55.27

GC content: 66.47

Gene sequence:

>1545_bases
ATGAAACGCTGGCTGCTCTATTGGTTCCTGGTAACTCCGCTGGCGGCGCAGAAGGCGGCGCCGTTTGAAGTGGAGGAGGC
GACGATCGCGCAGGTGCACGACGCGATGAAGGCCGGCCGGCTGACTTGCCGTGCGCTGGTCGGCATGTACCTAAAGCGGA
TCGAGGCGTACGACAAGAACGGGCCGGCGATCAACTCCATCGTGACGGTAAATCCGGACGTGGAGAGGCAGGCCGCGGAG
CTGGACGCGCGGTTCGCACAATCCGGGCTCACGGGGCCGCTGCACTGCGTGCCCGTGATCGTGAAAGACAACTTCGAGAC
TAAAGGGCTACAGACCACCGACGGGGCGCTGGCGTTAGCGGGCTATCTCCCCGAGAAGGACGCGTTCCAGGTAAAGCGCG
TGAAGGACGCCGGCGCGCTGGTGCTGGCGAAATCGAACATGGCGGAGTGGGCCTTCAGCCCGTACGAGACGGTGAACTCG
ATATTGCCCGGCTACACGCGGAACCCCTATGCTCTGGACCGGGTGACGGCCGGCTCGAGCGGCGGGACAGCGGCGGCGAT
TGCGGCGAGCCTCGGCCTGGTCGGGCTGGGCAGCGATACCGGCAACTCGATCCGCGGCCCGTCGTCGCACCAGGCGCTGG
CGGGGATCCGCTCGACGATGGGGCTGACCAGCCGCGCCGGCGTGCTGCCGCTCAGCATGCTCGCCGATATCGCAGGGCCG
ATGTGCCGCACGGTGGAAGACGCGGCCAGAGTGTTTCAGGTGATCGTGGGCGCGGATCCCGATGACGCGGTGACGGCGGC
GGCGAAGGCGCACCTGCCCCGCGACTACACGGCGGCCCTCAGGCGCGACGGATTGCGCGGGGCAGTCATCGGAGTGCTGC
GGCAGGCTTACGAGCGCGACACCACGGACCCGGAGATCGTGCGCATCTTCACGGCCGCGGTGGAGGATTTGAAGCGCGCC
GGGGCGACCATCGTGGACCCGGCGACGGTGGAAGGGCTGACGGAGATCCGGCGTCCGCGCGATGCGGGACCGTGCATGGG
CTTCAAGTACGACCTCAATCATTTTCTGGCGGCGCGGGGCGATCGGGTGCCGGTGAAGAACCTGACGGAGATCATCAAGT
CGGGACGCTTCCATCCCTCGGCGCAATTCCGGCTTGAAAGCGCGGAGAAGGGTCCGGAGAACGGACCGGATTCGCCGGGA
TGCGTAGCCGACCGCGAGTATCGCGAGAAGGTGCGTGAGGCGGTGGTGAAGACGATGGACCGGCTCAAGCTGGATGCGTT
CGTGTATCCAACGTGGAGCAATCCGCCGCGGCTGATCGGCGATTTAAATACGCCGGCGGGGGACAACAGCCAGTTCTATT
CGCCGACCACGGGCTTCCCGGCGATCAGCGTGCCGATGGGCTATTCACGCGGAGGCACGCTGCCGGCGGGAATGACGATC
TACGGCCGCGCGTGGAGCGAGGAGATCTTGATTCAGTATGCGTACGCGTATGAACAGGCGACGCACCACAGGCGACCGCC
GGCCTCGACGCCGCCACTGCGATGA

Upstream 100 bases:

>100_bases
CATCGCCTTCGCGCTTTTCCAGGACTGCAAGGCCTGCAGCATCGAAAGGCAATTATAGGTGATTGCGGAGAGCCGGCGCG
GGCACAGGTACAATAATGCG

Downstream 100 bases:

>100_bases
CCTAGGAAGGGTCCGGCGGTAAAGGGCGATCCGAGCGATTACGGGGAAGCCGCCTGAAAGGCGGCTGCAGCCAGGATTGG
CTGCCCCACAAGGAAGCAGG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKRWLLYWFLVTPLAAQKAAPFEVEEATIAQVHDAMKAGRLTCRALVGMYLKRIEAYDKNGPAINSIVTVNPDVERQAAE
LDARFAQSGLTGPLHCVPVIVKDNFETKGLQTTDGALALAGYLPEKDAFQVKRVKDAGALVLAKSNMAEWAFSPYETVNS
ILPGYTRNPYALDRVTAGSSGGTAAAIAASLGLVGLGSDTGNSIRGPSSHQALAGIRSTMGLTSRAGVLPLSMLADIAGP
MCRTVEDAARVFQVIVGADPDDAVTAAAKAHLPRDYTAALRRDGLRGAVIGVLRQAYERDTTDPEIVRIFTAAVEDLKRA
GATIVDPATVEGLTEIRRPRDAGPCMGFKYDLNHFLAARGDRVPVKNLTEIIKSGRFHPSAQFRLESAEKGPENGPDSPG
CVADREYREKVREAVVKTMDRLKLDAFVYPTWSNPPRLIGDLNTPAGDNSQFYSPTTGFPAISVPMGYSRGGTLPAGMTI
YGRAWSEEILIQYAYAYEQATHHRRPPASTPPLR

Sequences:

>Translated_514_residues
MKRWLLYWFLVTPLAAQKAAPFEVEEATIAQVHDAMKAGRLTCRALVGMYLKRIEAYDKNGPAINSIVTVNPDVERQAAE
LDARFAQSGLTGPLHCVPVIVKDNFETKGLQTTDGALALAGYLPEKDAFQVKRVKDAGALVLAKSNMAEWAFSPYETVNS
ILPGYTRNPYALDRVTAGSSGGTAAAIAASLGLVGLGSDTGNSIRGPSSHQALAGIRSTMGLTSRAGVLPLSMLADIAGP
MCRTVEDAARVFQVIVGADPDDAVTAAAKAHLPRDYTAALRRDGLRGAVIGVLRQAYERDTTDPEIVRIFTAAVEDLKRA
GATIVDPATVEGLTEIRRPRDAGPCMGFKYDLNHFLAARGDRVPVKNLTEIIKSGRFHPSAQFRLESAEKGPENGPDSPG
CVADREYREKVREAVVKTMDRLKLDAFVYPTWSNPPRLIGDLNTPAGDNSQFYSPTTGFPAISVPMGYSRGGTLPAGMTI
YGRAWSEEILIQYAYAYEQATHHRRPPASTPPLR
>Mature_514_residues
MKRWLLYWFLVTPLAAQKAAPFEVEEATIAQVHDAMKAGRLTCRALVGMYLKRIEAYDKNGPAINSIVTVNPDVERQAAE
LDARFAQSGLTGPLHCVPVIVKDNFETKGLQTTDGALALAGYLPEKDAFQVKRVKDAGALVLAKSNMAEWAFSPYETVNS
ILPGYTRNPYALDRVTAGSSGGTAAAIAASLGLVGLGSDTGNSIRGPSSHQALAGIRSTMGLTSRAGVLPLSMLADIAGP
MCRTVEDAARVFQVIVGADPDDAVTAAAKAHLPRDYTAALRRDGLRGAVIGVLRQAYERDTTDPEIVRIFTAAVEDLKRA
GATIVDPATVEGLTEIRRPRDAGPCMGFKYDLNHFLAARGDRVPVKNLTEIIKSGRFHPSAQFRLESAEKGPENGPDSPG
CVADREYREKVREAVVKTMDRLKLDAFVYPTWSNPPRLIGDLNTPAGDNSQFYSPTTGFPAISVPMGYSRGGTLPAGMTI
YGRAWSEEILIQYAYAYEQATHHRRPPASTPPLR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=265, Percent_Identity=34.7169811320755, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI195972892, Length=239, Percent_Identity=31.3807531380753, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=233, Percent_Identity=32.618025751073, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=246, Percent_Identity=30.0813008130081, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=194, Percent_Identity=30.9278350515464, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=230, Percent_Identity=26.9565217391304, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=158, Percent_Identity=30.379746835443, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=225, Percent_Identity=29.3333333333333, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=421, Percent_Identity=26.3657957244656, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=244, Percent_Identity=25, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=240, Percent_Identity=28.3333333333333, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648113, Length=271, Percent_Identity=32.4723247232472, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078093, Length=509, Percent_Identity=26.1296660117878, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24644968, Length=512, Percent_Identity=24.8046875, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356731, Length=250, Percent_Identity=32.8, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648435, Length=239, Percent_Identity=29.2887029288703, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648437, Length=239, Percent_Identity=29.2887029288703, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45550774, Length=239, Percent_Identity=29.2887029288703, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648441, Length=173, Percent_Identity=32.9479768786127, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648439, Length=173, Percent_Identity=32.9479768786127, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652985, Length=235, Percent_Identity=27.2340425531915, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922090, Length=235, Percent_Identity=27.2340425531915, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24652981, Length=235, Percent_Identity=27.2340425531915, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24652983, Length=235, Percent_Identity=27.2340425531915, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 55471; Mature: 55471

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWLLYWFLVTPLAAQKAAPFEVEEATIAQVHDAMKAGRLTCRALVGMYLKRIEAYDKN
CCHHEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GPAINSIVTVNPDVERQAAELDARFAQSGLTGPLHCVPVIVKDNFETKGLQTTDGALALA
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEE
GYLPEKDAFQVKRVKDAGALVLAKSNMAEWAFSPYETVNSILPGYTRNPYALDRVTAGSS
CCCCCCCCHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHHHCCCCCCCCCEEHEEECCCC
GGTAAAIAASLGLVGLGSDTGNSIRGPSSHQALAGIRSTMGLTSRAGVLPLSMLADIAGP
CCHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
MCRTVEDAARVFQVIVGADPDDAVTAAAKAHLPRDYTAALRRDGLRGAVIGVLRQAYERD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
TTDPEIVRIFTAAVEDLKRAGATIVDPATVEGLTEIRRPRDAGPCMGFKYDLNHFLAARG
CCCHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
DRVPVKNLTEIIKSGRFHPSAQFRLESAEKGPENGPDSPGCVADREYREKVREAVVKTMD
CCCCHHHHHHHHHCCCCCCCHHEEECCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RLKLDAFVYPTWSNPPRLIGDLNTPAGDNSQFYSPTTGFPAISVPMGYSRGGTLPAGMTI
HHEEEEEEECCCCCCCHHEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
YGRAWSEEILIQYAYAYEQATHHRRPPASTPPLR
ECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKRWLLYWFLVTPLAAQKAAPFEVEEATIAQVHDAMKAGRLTCRALVGMYLKRIEAYDKN
CCHHEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GPAINSIVTVNPDVERQAAELDARFAQSGLTGPLHCVPVIVKDNFETKGLQTTDGALALA
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEE
GYLPEKDAFQVKRVKDAGALVLAKSNMAEWAFSPYETVNSILPGYTRNPYALDRVTAGSS
CCCCCCCCHHHHHHCCCCEEEEEECCCHHHCCCHHHHHHHHCCCCCCCCCEEHEEECCCC
GGTAAAIAASLGLVGLGSDTGNSIRGPSSHQALAGIRSTMGLTSRAGVLPLSMLADIAGP
CCHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
MCRTVEDAARVFQVIVGADPDDAVTAAAKAHLPRDYTAALRRDGLRGAVIGVLRQAYERD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
TTDPEIVRIFTAAVEDLKRAGATIVDPATVEGLTEIRRPRDAGPCMGFKYDLNHFLAARG
CCCHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
DRVPVKNLTEIIKSGRFHPSAQFRLESAEKGPENGPDSPGCVADREYREKVREAVVKTMD
CCCCHHHHHHHHHCCCCCCCHHEEECCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RLKLDAFVYPTWSNPPRLIGDLNTPAGDNSQFYSPTTGFPAISVPMGYSRGGTLPAGMTI
HHEEEEEEECCCCCCCHHEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
YGRAWSEEILIQYAYAYEQATHHRRPPASTPPLR
ECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]