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The map label for this gene is yogA [H]

Identifier: 116623356

GI number: 116623356

Start: 5397357

End: 5398226

Strand: Reverse

Name: yogA [H]

Synonym: Acid_4265

Alternate gene names: 116623356

Gene position: 5398226-5397357 (Counterclockwise)

Preceding gene: 116623359

Following gene: 116623355

Centisome position: 54.17

GC content: 65.06

Gene sequence:

>870_bases
GTGCTGGTAGAAGGGCGGGCGCCGATGCCGGTACCGGGAGAGGGCGAACTGTTGATTCGCGTGCTAGCGGCGGGGATTAC
TACGACGGAGCTGGGCTGGTATCCGACGACGCATACTAAGAGCGGAGAAACCCGCAAGGGGGCGGTGCCGACGCACGAAT
TCTCGGGCGTGATCGCGGACGGGCCGGAGAAGGGGCACCCAGTCTTCGGGATGAATGACTGGTATGCGGAAGGCGCGCTG
GCCGAGTATTGCACCGCGCCGGTGGCGTCGGTGGCGGCCAAGCCGCAGCGGTTGACGCACGAGGAGGCGGCATCGGTGCC
GATCGGGGCGTTGACGGCGTGGCAGGGCTTGTACGATCGCGCGAAGCTGCGGGCGGGGGAGCGCGTGCTGGTGCACGGCG
GGTCGGGCGCGGTGGGGGTGTTCGCGATTCAACTGGCGCGGTTGCGGGGCGCGCACGTGATCGCGACGGCATCGGCGCGC
CATCTGGATTTCGTGCGGGCACTGGGAGCGGAGGAATCGGTAGACTACCGGGGGACTCCGTATAAGGACAAGGTGGATGT
GGTTTTCGATACGGTGGGTGGTGAGACACTTGCGCGATCCTGGAGCCTGCTGGGTGAGGGGGGACGGATGGTGACGATTG
TGTCGGGGGCAGAGGACTCGGCGGAGGCGCGGGAGAGAGAGGCGTTCTTCATTGTCGAGCCGAACCGGAAGCAACTTACG
GAAGTCGCGGAGTTACTGCAGGGCGGGCAACTTACTCCTGTGGTAGATACGGTAATCCCGCTAAGTTGGGCGGCGGAAGC
TTATACAGGGCAGTTGACGCGGAGCGGGCGAGGGAGAGTGGTGGTGGCGATAGAAAAGAGTGAACGATGA

Upstream 100 bases:

>100_bases
CGAGCGCATAAACGGATGACGGGGCTGGGGTCGGGAAATTAGTTGGAGTAGGATGGGAAAAATGAAGGTCATGCATTTGC
GGGATGATTCCGAGGGTCCG

Downstream 100 bases:

>100_bases
AGAGACTGGATGGGAAGCGCGCTTTGATTACCGGGGGGACGACCGGGATTGGATTGGAAACGGCGCGCCTCTTCATGGAG
GAAGGCGCGCGGGTCGCAAT

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLVEGRAPMPVPGEGELLIRVLAAGITTTELGWYPTTHTKSGETRKGAVPTHEFSGVIADGPEKGHPVFGMNDWYAEGAL
AEYCTAPVASVAAKPQRLTHEEAASVPIGALTAWQGLYDRAKLRAGERVLVHGGSGAVGVFAIQLARLRGAHVIATASAR
HLDFVRALGAEESVDYRGTPYKDKVDVVFDTVGGETLARSWSLLGEGGRMVTIVSGAEDSAEAREREAFFIVEPNRKQLT
EVAELLQGGQLTPVVDTVIPLSWAAEAYTGQLTRSGRGRVVVAIEKSER

Sequences:

>Translated_289_residues
MLVEGRAPMPVPGEGELLIRVLAAGITTTELGWYPTTHTKSGETRKGAVPTHEFSGVIADGPEKGHPVFGMNDWYAEGAL
AEYCTAPVASVAAKPQRLTHEEAASVPIGALTAWQGLYDRAKLRAGERVLVHGGSGAVGVFAIQLARLRGAHVIATASAR
HLDFVRALGAEESVDYRGTPYKDKVDVVFDTVGGETLARSWSLLGEGGRMVTIVSGAEDSAEAREREAFFIVEPNRKQLT
EVAELLQGGQLTPVVDTVIPLSWAAEAYTGQLTRSGRGRVVVAIEKSER
>Mature_289_residues
MLVEGRAPMPVPGEGELLIRVLAAGITTTELGWYPTTHTKSGETRKGAVPTHEFSGVIADGPEKGHPVFGMNDWYAEGAL
AEYCTAPVASVAAKPQRLTHEEAASVPIGALTAWQGLYDRAKLRAGERVLVHGGSGAVGVFAIQLARLRGAHVIATASAR
HLDFVRALGAEESVDYRGTPYKDKVDVVFDTVGGETLARSWSLLGEGGRMVTIVSGAEDSAEAREREAFFIVEPNRKQLT
EVAELLQGGQLTPVVDTVIPLSWAAEAYTGQLTRSGRGRVVVAIEKSER

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=301, Percent_Identity=29.9003322259136, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI13236495, Length=301, Percent_Identity=29.9003322259136, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI47519420, Length=254, Percent_Identity=31.1023622047244, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI194239676, Length=288, Percent_Identity=29.8611111111111, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI18379349, Length=245, Percent_Identity=28.1632653061224, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI28557745, Length=223, Percent_Identity=28.6995515695067, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI22538446, Length=316, Percent_Identity=26.8987341772152, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI22538444, Length=316, Percent_Identity=26.8987341772152, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI197927207, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI24308257, Length=330, Percent_Identity=26.3636363636364, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507255, Length=305, Percent_Identity=26.5573770491803, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 30812; Mature: 30812

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVEGRAPMPVPGEGELLIRVLAAGITTTELGWYPTTHTKSGETRKGAVPTHEFSGVIAD
CEECCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCHHCCCEEEC
GPEKGHPVFGMNDWYAEGALAEYCTAPVASVAAKPQRLTHEEAASVPIGALTAWQGLYDR
CCCCCCCEECCCCHHHCCCHHHHHHCHHHHHHCCCHHHCHHHHHCCCHHHHHHHHHHHHH
AKLRAGERVLVHGGSGAVGVFAIQLARLRGAHVIATASARHLDFVRALGAEESVDYRGTP
HHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCC
YKDKVDVVFDTVGGETLARSWSLLGEGGRMVTIVSGAEDSAEAREREAFFIVEPNRKQLT
CCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCEEEEECCCHHHHH
EVAELLQGGQLTPVVDTVIPLSWAAEAYTGQLTRSGRGRVVVAIEKSER
HHHHHHHCCCCCHHHHHHHCHHHHHHHHHCHHEECCCCEEEEEEECCCC
>Mature Secondary Structure
MLVEGRAPMPVPGEGELLIRVLAAGITTTELGWYPTTHTKSGETRKGAVPTHEFSGVIAD
CEECCCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCHHCCCEEEC
GPEKGHPVFGMNDWYAEGALAEYCTAPVASVAAKPQRLTHEEAASVPIGALTAWQGLYDR
CCCCCCCEECCCCHHHCCCHHHHHHCHHHHHHCCCHHHCHHHHHCCCHHHHHHHHHHHHH
AKLRAGERVLVHGGSGAVGVFAIQLARLRGAHVIATASARHLDFVRALGAEESVDYRGTP
HHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCC
YKDKVDVVFDTVGGETLARSWSLLGEGGRMVTIVSGAEDSAEAREREAFFIVEPNRKQLT
CCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCEEEEECCCHHHHH
EVAELLQGGQLTPVVDTVIPLSWAAEAYTGQLTRSGRGRVVVAIEKSER
HHHHHHHCCCCCHHHHHHHCHHHHHHHHHCHHEECCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]