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The map label for this gene is asnB [H]

Identifier: 116623198

GI number: 116623198

Start: 5215443

End: 5217362

Strand: Direct

Name: asnB [H]

Synonym: Acid_4105

Alternate gene names: 116623198

Gene position: 5215443-5217362 (Clockwise)

Preceding gene: 116623193

Following gene: 116623199

Centisome position: 52.33

GC content: 61.82

Gene sequence:

>1920_bases
ATGTGCGGAATCGCCGGGTTCACTCGTCTCAATAAGTATGCCAGCCGGGGTGTAGCGCAGCGAATTACGGAAGCGCTCTC
ACATCGCGGGCCGGACCAACAGGGAGTCTTCGAGGGAAGCGAAGCGACACTGTGCGCCGTGCGGCTGAAGATCATCGACC
TGGCGGGCGGGGATCAGCCGATTCTCAGCGACGATGGCGACACGGCGATCGCCTTTAACGGGGAAATCTACAATCATCGC
GAGGTGCGCGGCGAGCTGGAAGCTCTGGGACACAGATTCCGTTCGAATTGCGACACGGAGACAGTGCTGCGCGCGTTCCT
GCAGTGGGACACGGCGGCGTTTCAGCGGCTGCGAGGCATGTTTGCGATCGCGCTGTGGACGGAATCACGGAAGCGGTTGC
TGCTGGTTCGCGACCGGATGGGGATCAAGCCGCTGTACTACTACCGGAGCGGCGACGATCTCTATTTCGGCTCCGAATTA
AAGGCCATCCTTGAACATCCGGACGTGCCGCGGACCCTGGACCTGAGCGCGCTGGACACGTATCTCTCGGTGAACTATGT
GCCGGGTCCGCAAACGCTGATTCGGGGAATCCGGAAGGTCCCCCCGGGGCACCTGCTGGAATGGCGGCACGGCAAGGCGT
GGTGCGAGGCGTGGTGGAAGCGGCCGGATTCGGGGACACGCCGGATTTCGCTGGGGGCGGCGAAGGAACAACTCGACGTA
CTGATGAAGGATTCGGTGCGTGAGCACCTGGTGTCGGATGTTCCGTTGGGTGTCTGGGCGTCGGGCGGATTGGACTCGTC
GGCGATCCTGCACTATGCCGCGGCGCAGACCGCGGGGCGGCTGAAGACGTTCTCGGTTTCGTTCGAGGGACGGAGTTTCG
ACGAGAGCCGATATTTCCGAGAGATCGCGCAGGTATACGGCACGGATCATCACGAGTTCGATTTGAACCCGGATGTGGAA
CTGCAGAGCGCGATCGAGGATTTCGCGTACTACTCCGACGAACCGAGCGCGGACGCGGGCGCGCTGCCGGTGTGGTTTCT
CTCGCGCATGAGCCGGCGTCACGTAACGGTGGCGCTTTCGGGCGAGGGCGCCGACGAGTTGTTCGGCGGCTATCTGACGT
ACCAGGCGGACAGACTGGCGCGGCCGTTCCGGATGGTGCCGGCGGGATTGCGGCGGTTGATGCGGGGCGCGTTGGACCGG
TACATGCCAGTGTCCGACGACAAGATCAGCCTGGAATACAAACTGAAGCGCTGGATCGACGGCAGCCTGTTGAATGCCGA
CGAGGCGCACTTCTTCTGGAACGGGACCTTCTCGCCGGAGCAGTTGCGGCAGATCCGTCCGGGCACCAACGGGAACGGAT
TACAGCGCATGGTCGAAGGACTGAACGGTCCGCGCGAGGTGGTCCAGCGTTATCTCGACGTGGACCAGAGCTTCTATCTT
CCGGACGATATTTTGTACAAGACGGATCGGATGAGCATGGCGCACTCGCTGGAAGTGCGGCCGCCCTTCCTGGATCACCG
GATCGTGGAATTCGCGGCTTCCCTGCCCCAGCGGCTGAAGATCCGCGGCGCAAAGCAGAAGCATGTGCTGCGCGAACTGA
TGCGCGGGAAACTGCCGGAAAGCGTGCTGACGCGCAAGAAGAGCGGGTTCGACATACCGACGCACGACTGGTTCCGCGGA
ACGCTTCGCGGAATGCTGATGGATACACTGACGCCGGACGCGATCGCAGCGACCGGCGTGTTCGACGCGAACGCAATCGA
CGGCTTGATACGCGATCATATGGAGAGGCGTATCAACGTCGGATATCACTTATGGGGCCTGCTGACGCTTTTCCTGTGGA
TGAAAAGATGGAAGGTGGAGGCATTGCCTCCCGAGGAAGAGATGCGTCAGGTTCCGGCGCGAGTTCTCGCTACCAGATAA

Upstream 100 bases:

>100_bases
ATGAAGTGTTTCGGGAAGGCATTGTGGTATATTTAACGAAAATCCATCAATAGAATGGAATCTAGATGTATTTTCTAGGG
AACGGGGACAGCTACGCAGC

Downstream 100 bases:

>100_bases
TCGTACTGCTGGTGGCGGCTGCGATTTTCCTGGGCTGTATTGTCAGCCCGCCATCGTTAATGGATGACGTGGACGCCGTA
CAGGCGCAGATTGGGCGGAA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MCGIAGFTRLNKYASRGVAQRITEALSHRGPDQQGVFEGSEATLCAVRLKIIDLAGGDQPILSDDGDTAIAFNGEIYNHR
EVRGELEALGHRFRSNCDTETVLRAFLQWDTAAFQRLRGMFAIALWTESRKRLLLVRDRMGIKPLYYYRSGDDLYFGSEL
KAILEHPDVPRTLDLSALDTYLSVNYVPGPQTLIRGIRKVPPGHLLEWRHGKAWCEAWWKRPDSGTRRISLGAAKEQLDV
LMKDSVREHLVSDVPLGVWASGGLDSSAILHYAAAQTAGRLKTFSVSFEGRSFDESRYFREIAQVYGTDHHEFDLNPDVE
LQSAIEDFAYYSDEPSADAGALPVWFLSRMSRRHVTVALSGEGADELFGGYLTYQADRLARPFRMVPAGLRRLMRGALDR
YMPVSDDKISLEYKLKRWIDGSLLNADEAHFFWNGTFSPEQLRQIRPGTNGNGLQRMVEGLNGPREVVQRYLDVDQSFYL
PDDILYKTDRMSMAHSLEVRPPFLDHRIVEFAASLPQRLKIRGAKQKHVLRELMRGKLPESVLTRKKSGFDIPTHDWFRG
TLRGMLMDTLTPDAIAATGVFDANAIDGLIRDHMERRINVGYHLWGLLTLFLWMKRWKVEALPPEEEMRQVPARVLATR

Sequences:

>Translated_639_residues
MCGIAGFTRLNKYASRGVAQRITEALSHRGPDQQGVFEGSEATLCAVRLKIIDLAGGDQPILSDDGDTAIAFNGEIYNHR
EVRGELEALGHRFRSNCDTETVLRAFLQWDTAAFQRLRGMFAIALWTESRKRLLLVRDRMGIKPLYYYRSGDDLYFGSEL
KAILEHPDVPRTLDLSALDTYLSVNYVPGPQTLIRGIRKVPPGHLLEWRHGKAWCEAWWKRPDSGTRRISLGAAKEQLDV
LMKDSVREHLVSDVPLGVWASGGLDSSAILHYAAAQTAGRLKTFSVSFEGRSFDESRYFREIAQVYGTDHHEFDLNPDVE
LQSAIEDFAYYSDEPSADAGALPVWFLSRMSRRHVTVALSGEGADELFGGYLTYQADRLARPFRMVPAGLRRLMRGALDR
YMPVSDDKISLEYKLKRWIDGSLLNADEAHFFWNGTFSPEQLRQIRPGTNGNGLQRMVEGLNGPREVVQRYLDVDQSFYL
PDDILYKTDRMSMAHSLEVRPPFLDHRIVEFAASLPQRLKIRGAKQKHVLRELMRGKLPESVLTRKKSGFDIPTHDWFRG
TLRGMLMDTLTPDAIAATGVFDANAIDGLIRDHMERRINVGYHLWGLLTLFLWMKRWKVEALPPEEEMRQVPARVLATR
>Mature_639_residues
MCGIAGFTRLNKYASRGVAQRITEALSHRGPDQQGVFEGSEATLCAVRLKIIDLAGGDQPILSDDGDTAIAFNGEIYNHR
EVRGELEALGHRFRSNCDTETVLRAFLQWDTAAFQRLRGMFAIALWTESRKRLLLVRDRMGIKPLYYYRSGDDLYFGSEL
KAILEHPDVPRTLDLSALDTYLSVNYVPGPQTLIRGIRKVPPGHLLEWRHGKAWCEAWWKRPDSGTRRISLGAAKEQLDV
LMKDSVREHLVSDVPLGVWASGGLDSSAILHYAAAQTAGRLKTFSVSFEGRSFDESRYFREIAQVYGTDHHEFDLNPDVE
LQSAIEDFAYYSDEPSADAGALPVWFLSRMSRRHVTVALSGEGADELFGGYLTYQADRLARPFRMVPAGLRRLMRGALDR
YMPVSDDKISLEYKLKRWIDGSLLNADEAHFFWNGTFSPEQLRQIRPGTNGNGLQRMVEGLNGPREVVQRYLDVDQSFYL
PDDILYKTDRMSMAHSLEVRPPFLDHRIVEFAASLPQRLKIRGAKQKHVLRELMRGKLPESVLTRKKSGFDIPTHDWFRG
TLRGMLMDTLTPDAIAATGVFDANAIDGLIRDHMERRINVGYHLWGLLTLFLWMKRWKVEALPPEEEMRQVPARVLATR

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI296010848, Length=370, Percent_Identity=25.4054054054054, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI168229248, Length=373, Percent_Identity=25.4691689008043, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI168229252, Length=373, Percent_Identity=25.4691689008043, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI168229250, Length=373, Percent_Identity=25.4691689008043, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI296010852, Length=303, Percent_Identity=24.0924092409241, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI296010850, Length=303, Percent_Identity=24.0924092409241, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1786889, Length=396, Percent_Identity=31.0606060606061, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71993933, Length=490, Percent_Identity=28.1632653061224, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147557, Length=490, Percent_Identity=28.1632653061224, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147560, Length=310, Percent_Identity=29.0322580645161, Blast_Score=102, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6325403, Length=414, Percent_Identity=28.9855072463768, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321563, Length=415, Percent_Identity=27.9518072289157, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI45553209, Length=398, Percent_Identity=30.4020100502513, Blast_Score=148, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72657; Mature: 72657

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGFTRLNKYASRGVAQRITEALSHRGPDQQGVFEGSEATLCAVRLKIIDLAGGDQP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCC
ILSDDGDTAIAFNGEIYNHREVRGELEALGHRFRSNCDTETVLRAFLQWDTAAFQRLRGM
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FAIALWTESRKRLLLVRDRMGIKPLYYYRSGDDLYFGSELKAILEHPDVPRTLDLSALDT
EEEEEEECCCCEEEEEECCCCCCEEEEEECCCCEEECHHHHHHHCCCCCCCEECHHHHHH
YLSVNYVPGPQTLIRGIRKVPPGHLLEWRHGKAWCEAWWKRPDSGTRRISLGAAKEQLDV
HHEEECCCCHHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHH
LMKDSVREHLVSDVPLGVWASGGLDSSAILHYAAAQTAGRLKTFSVSFEGRSFDESRYFR
HHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHH
EIAQVYGTDHHEFDLNPDVELQSAIEDFAYYSDEPSADAGALPVWFLSRMSRRHVTVALS
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEC
GEGADELFGGYLTYQADRLARPFRMVPAGLRRLMRGALDRYMPVSDDKISLEYKLKRWID
CCCHHHHHCCHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHCC
GSLLNADEAHFFWNGTFSPEQLRQIRPGTNGNGLQRMVEGLNGPREVVQRYLDVDQSFYL
CCCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
PDDILYKTDRMSMAHSLEVRPPFLDHRIVEFAASLPQRLKIRGAKQKHVLRELMRGKLPE
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCH
SVLTRKKSGFDIPTHDWFRGTLRGMLMDTLTPDAIAATGVFDANAIDGLIRDHMERRINV
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCC
GYHLWGLLTLFLWMKRWKVEALPPEEEMRQVPARVLATR
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGIAGFTRLNKYASRGVAQRITEALSHRGPDQQGVFEGSEATLCAVRLKIIDLAGGDQP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCC
ILSDDGDTAIAFNGEIYNHREVRGELEALGHRFRSNCDTETVLRAFLQWDTAAFQRLRGM
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FAIALWTESRKRLLLVRDRMGIKPLYYYRSGDDLYFGSELKAILEHPDVPRTLDLSALDT
EEEEEEECCCCEEEEEECCCCCCEEEEEECCCCEEECHHHHHHHCCCCCCCEECHHHHHH
YLSVNYVPGPQTLIRGIRKVPPGHLLEWRHGKAWCEAWWKRPDSGTRRISLGAAKEQLDV
HHEEECCCCHHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHH
LMKDSVREHLVSDVPLGVWASGGLDSSAILHYAAAQTAGRLKTFSVSFEGRSFDESRYFR
HHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHH
EIAQVYGTDHHEFDLNPDVELQSAIEDFAYYSDEPSADAGALPVWFLSRMSRRHVTVALS
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEC
GEGADELFGGYLTYQADRLARPFRMVPAGLRRLMRGALDRYMPVSDDKISLEYKLKRWID
CCCHHHHHCCHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHCC
GSLLNADEAHFFWNGTFSPEQLRQIRPGTNGNGLQRMVEGLNGPREVVQRYLDVDQSFYL
CCCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
PDDILYKTDRMSMAHSLEVRPPFLDHRIVEFAASLPQRLKIRGAKQKHVLRELMRGKLPE
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCH
SVLTRKKSGFDIPTHDWFRGTLRGMLMDTLTPDAIAATGVFDANAIDGLIRDHMERRINV
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCC
GYHLWGLLTLFLWMKRWKVEALPPEEEMRQVPARVLATR
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]