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The map label for this gene is leuD

Identifier: 116623164

GI number: 116623164

Start: 5166897

End: 5167472

Strand: Direct

Name: leuD

Synonym: Acid_4070

Alternate gene names: 116623164

Gene position: 5166897-5167472 (Clockwise)

Preceding gene: 116623163

Following gene: 116623165

Centisome position: 51.85

GC content: 60.94

Gene sequence:

>576_bases
ATGATTCCGTTCACCAATTTCGAAAGCCGCCTGATCAAGCTGCCCATCAATAACGTCGATACCGACCAGATTATCCCGGC
CCGATTTTTGAAGACCACGTCCAAGGTCGGGCTCGATAAACAGCTCTTTAACGACTGGCGGCAGGACCCGGATTTCGTCC
TGAACCGCCCCGAGTCGCAGGGCCTGCAAATCTTGCTGGCGGGCGATAACTTCGGCTGCGGCAGTTCCCGCGAACATGCT
CCGTGGGCGCTGACCCAGTGGGGCTTCCGCGCCGTCATCTCCACCAGCTTCGCCGACATCTTCAAGCAGAATTCCCTGAA
GAACAGCCTGCTGCCCATCGAAGTCCCGGCCGACGTCCACGCCGAACTCTTCTCTTCGGACGGCCCGGCAAAGATCGACC
TGCCCAACCAGACTCTGACGACCCCCTCGGGCCGCGAAGTCCATTTCGAAGTGGACCAGTTCTCCAAGCACTGCCTCGTG
AAGGGCGTGGACGAGCTCGGCTACATCCTCGAGCAGGCCCCCGAAATCGCCGCCTACGAGGCCTCGCACCCCGCCCCAAT
CAACGCGCTGGCGTAA

Upstream 100 bases:

>100_bases
AAGGACGGCAGGGCAAGGGCGGCCGCACCTTCCTGGCCAGCCCGCTGACCGCCGCCGCCAGCGCCATCACCGGCGTCGTG
ACCGATGTGAGGACTCTCCT

Downstream 100 bases:

>100_bases
CGCGAGGTATGCCTCCGTGCTTCTCTTTCAAGCACCGGACGAAGACACCCGACCGAGCCGTGACCGTAAAGTAAGGGAGC
GGTTGCGCGCCGAAAATTGA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MIPFTNFESRLIKLPINNVDTDQIIPARFLKTTSKVGLDKQLFNDWRQDPDFVLNRPESQGLQILLAGDNFGCGSSREHA
PWALTQWGFRAVISTSFADIFKQNSLKNSLLPIEVPADVHAELFSSDGPAKIDLPNQTLTTPSGREVHFEVDQFSKHCLV
KGVDELGYILEQAPEIAAYEASHPAPINALA

Sequences:

>Translated_191_residues
MIPFTNFESRLIKLPINNVDTDQIIPARFLKTTSKVGLDKQLFNDWRQDPDFVLNRPESQGLQILLAGDNFGCGSSREHA
PWALTQWGFRAVISTSFADIFKQNSLKNSLLPIEVPADVHAELFSSDGPAKIDLPNQTLTTPSGREVHFEVDQFSKHCLV
KGVDELGYILEQAPEIAAYEASHPAPINALA
>Mature_191_residues
MIPFTNFESRLIKLPINNVDTDQIIPARFLKTTSKVGLDKQLFNDWRQDPDFVLNRPESQGLQILLAGDNFGCGSSREHA
PWALTQWGFRAVISTSFADIFKQNSLKNSLLPIEVPADVHAELFSSDGPAKIDLPNQTLTTPSGREVHFEVDQFSKHCLV
KGVDELGYILEQAPEIAAYEASHPAPINALA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=44.1624365482233, Blast_Score=167, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321429, Length=197, Percent_Identity=44.1624365482233, Blast_Score=160, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6320440, Length=113, Percent_Identity=34.5132743362832, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_SOLUE (Q01Z80)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_825320.1
- ProteinModelPortal:   Q01Z80
- SMR:   Q01Z80
- STRING:   Q01Z80
- GeneID:   4427669
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_4070
- NMPDR:   fig|234267.9.peg.3867
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   NCGMLAI
- PhylomeDB:   Q01Z80
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21200; Mature: 21200

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFTNFESRLIKLPINNVDTDQIIPARFLKTTSKVGLDKQLFNDWRQDPDFVLNRPESQ
CCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEECCCCC
GLQILLAGDNFGCGSSREHAPWALTQWGFRAVISTSFADIFKQNSLKNSLLPIEVPADVH
CEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHH
AELFSSDGPAKIDLPNQTLTTPSGREVHFEVDQFSKHCLVKGVDELGYILEQAPEIAAYE
HHHHCCCCCEEEECCCCEECCCCCCEEEEEHHHHHHHHHHHCHHHHHHHHHHCCCCEEEC
ASHPAPINALA
CCCCCCCCCCC
>Mature Secondary Structure
MIPFTNFESRLIKLPINNVDTDQIIPARFLKTTSKVGLDKQLFNDWRQDPDFVLNRPESQ
CCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEECCCCC
GLQILLAGDNFGCGSSREHAPWALTQWGFRAVISTSFADIFKQNSLKNSLLPIEVPADVH
CEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHH
AELFSSDGPAKIDLPNQTLTTPSGREVHFEVDQFSKHCLVKGVDELGYILEQAPEIAAYE
HHHHCCCCCEEEECCCCEECCCCCCEEEEEHHHHHHHHHHHCHHHHHHHHHHCCCCEEEC
ASHPAPINALA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA