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The map label for this gene is leuC

Identifier: 116623163

GI number: 116623163

Start: 5165500

End: 5166900

Strand: Direct

Name: leuC

Synonym: Acid_4069

Alternate gene names: 116623163

Gene position: 5165500-5166900 (Clockwise)

Preceding gene: 116623162

Following gene: 116623164

Centisome position: 51.83

GC content: 65.81

Gene sequence:

>1401_bases
ATGCCACGTACGATCATCGAAAAGCTTTGGGATTCCCACGTAGTTCACGAGCGCCCCGGCGCGCCCACGCTGCTCTTTAT
CGACCTCCACCTGGTACACGAAGTGACGTCGCCGCAGGCCTTCCAGGGCTTGCGCGAGCGCGGCTTGAAAGTGCGCCGCC
CCGATCTCACCATCGCCACCGCGGACCACAGCATTCCGACTACGGACCGCTCTCTGCCGATCGTAGACGAGATCGCGGCC
AAACAGCTGGCGCAGCTCGAGACCAACTGCAAGGATTTCGGCATTCGCTGCCTCGGCGTTCATAGCAGCCAGCAGGGCAT
CGTGCACGTGATCGGGCCCGAGCTAGGCCTGACCCAGCCCGGCATGACCGTGGTCTGCGGCGATAGCCACACGGCCACCC
ACGGCGCGTTCGGGGCACTCGCCTTCGGAATAGGCACCAGCGAAGTGGAACAGGTGCTGGCCAGCCAGTGCCTGCTGCAG
CGCAAGTCGAAGACCTTCCAGGTGAAGGTGGATGGCGCGCTGAAAGCCGGCGTGTCGGCCAAGGATATTATTCTCGCGCT
GATCGCCCGGATCGGCATCGGCGGCGGCACCGGCAGCGTCTTCGAATATACCGGCAGCGCCATCCGCGCGCTCTCCGTGG
AAGAGCGCATGACGGTCTGCAACATGTCGATCGAGGGCGGCGCGCGCGCCGGCCTGATTGCGCCTGACGATGCCACGTTC
GAATATCTCGCGGGTCGTCCGCACGCGCCGAAAGGCGCCGCCTGGGACGCCGCTATCCCGCGCTGGAAGCAGCTGCCCAC
CGATGACGGCGCGACCTACGATCGCAGCGTCTCGATCGACGCCGATACGCTCGAGCCGATGATCACCTATGGCACCAATC
CCGGGATGGGCGTCTCGATCAATGCGGCGCTCCCAGACCCATCGCAAGTCAGCGACCCGATGGCGCGCGATTCCATCACC
AAGGCGCTGGCGTACATGGGACTCGAAGGCGGCAAGCCGCTGGTCGGCCACCCCATCGACGTAGTCTTCATCGGCAGCTG
CACCAATTCGCGGATCAGCGATCTGCGTACCGCCGCCGGCTTGCTGAAGGGCCGCAAGGTCAGCCCCAAGGTTCGCGTGA
TGGTGGTGCCGGGCTCGCAGGAGGTCAAGCGCCAGGCCATCGCCGAGGGGCTGCCCGAGATTTTCCGCGCCGCCGGCTGC
GATTACCGCGAGCCCGGCTGCAGCATGTGCATCGCGATGAATGGTGACCAGCTCGCGCCCGGCGAGTACAGCGTTTCCAC
CAGCAATCGCAACTTCGAAGGACGGCAGGGCAAGGGCGGCCGCACCTTCCTGGCCAGCCCGCTGACCGCCGCCGCCAGCG
CCATCACCGGCGTCGTGACCGATGTGAGGACTCTCCTATGA

Upstream 100 bases:

>100_bases
CACAGGTTGGTCAAGCGGTCTGCGCCGCAATCGGCTGAGCACGCGGATTACCATTCCGCGCGCTCAGCCATAATAGAAAT
AGCCCCCACCGAGTTTTCTT

Downstream 100 bases:

>100_bases
TTCCGTTCACCAATTTCGAAAGCCGCCTGATCAAGCTGCCCATCAATAACGTCGATACCGACCAGATTATCCCGGCCCGA
TTTTTGAAGACCACGTCCAA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPRTIIEKLWDSHVVHERPGAPTLLFIDLHLVHEVTSPQAFQGLRERGLKVRRPDLTIATADHSIPTTDRSLPIVDEIAA
KQLAQLETNCKDFGIRCLGVHSSQQGIVHVIGPELGLTQPGMTVVCGDSHTATHGAFGALAFGIGTSEVEQVLASQCLLQ
RKSKTFQVKVDGALKAGVSAKDIILALIARIGIGGGTGSVFEYTGSAIRALSVEERMTVCNMSIEGGARAGLIAPDDATF
EYLAGRPHAPKGAAWDAAIPRWKQLPTDDGATYDRSVSIDADTLEPMITYGTNPGMGVSINAALPDPSQVSDPMARDSIT
KALAYMGLEGGKPLVGHPIDVVFIGSCTNSRISDLRTAAGLLKGRKVSPKVRVMVVPGSQEVKRQAIAEGLPEIFRAAGC
DYREPGCSMCIAMNGDQLAPGEYSVSTSNRNFEGRQGKGGRTFLASPLTAAASAITGVVTDVRTLL

Sequences:

>Translated_466_residues
MPRTIIEKLWDSHVVHERPGAPTLLFIDLHLVHEVTSPQAFQGLRERGLKVRRPDLTIATADHSIPTTDRSLPIVDEIAA
KQLAQLETNCKDFGIRCLGVHSSQQGIVHVIGPELGLTQPGMTVVCGDSHTATHGAFGALAFGIGTSEVEQVLASQCLLQ
RKSKTFQVKVDGALKAGVSAKDIILALIARIGIGGGTGSVFEYTGSAIRALSVEERMTVCNMSIEGGARAGLIAPDDATF
EYLAGRPHAPKGAAWDAAIPRWKQLPTDDGATYDRSVSIDADTLEPMITYGTNPGMGVSINAALPDPSQVSDPMARDSIT
KALAYMGLEGGKPLVGHPIDVVFIGSCTNSRISDLRTAAGLLKGRKVSPKVRVMVVPGSQEVKRQAIAEGLPEIFRAAGC
DYREPGCSMCIAMNGDQLAPGEYSVSTSNRNFEGRQGKGGRTFLASPLTAAASAITGVVTDVRTLL
>Mature_465_residues
PRTIIEKLWDSHVVHERPGAPTLLFIDLHLVHEVTSPQAFQGLRERGLKVRRPDLTIATADHSIPTTDRSLPIVDEIAAK
QLAQLETNCKDFGIRCLGVHSSQQGIVHVIGPELGLTQPGMTVVCGDSHTATHGAFGALAFGIGTSEVEQVLASQCLLQR
KSKTFQVKVDGALKAGVSAKDIILALIARIGIGGGTGSVFEYTGSAIRALSVEERMTVCNMSIEGGARAGLIAPDDATFE
YLAGRPHAPKGAAWDAAIPRWKQLPTDDGATYDRSVSIDADTLEPMITYGTNPGMGVSINAALPDPSQVSDPMARDSITK
ALAYMGLEGGKPLVGHPIDVVFIGSCTNSRISDLRTAAGLLKGRKVSPKVRVMVVPGSQEVKRQAIAEGLPEIFRAAGCD
YREPGCSMCIAMNGDQLAPGEYSVSTSNRNFEGRQGKGGRTFLASPLTAAASAITGVVTDVRTLL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=372, Percent_Identity=27.6881720430108, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI41352693, Length=377, Percent_Identity=24.6684350132626, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=58.2795698924731, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI1787531, Length=374, Percent_Identity=27.807486631016, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI2367097, Length=108, Percent_Identity=38.8888888888889, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87081781, Length=325, Percent_Identity=24.6153846153846, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=371, Percent_Identity=30.4582210242588, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=371, Percent_Identity=30.4582210242588, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=30.8196721311475, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=455, Percent_Identity=25.4945054945055, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=53.927813163482, Blast_Score=507, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6320440, Length=429, Percent_Identity=28.2051282051282, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=411, Percent_Identity=28.2238442822384, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=369, Percent_Identity=29.5392953929539, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281365315, Length=382, Percent_Identity=28.5340314136126, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17864292, Length=382, Percent_Identity=28.5340314136126, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=28.5340314136126, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=474, Percent_Identity=26.5822784810127, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645686, Length=462, Percent_Identity=26.6233766233766, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17137564, Length=368, Percent_Identity=27.445652173913, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SOLUE (Q01Z81)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_825319.1
- ProteinModelPortal:   Q01Z81
- SMR:   Q01Z81
- STRING:   Q01Z81
- GeneID:   4427668
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_4069
- NMPDR:   fig|234267.9.peg.3866
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q01Z81
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49283; Mature: 49152

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTIIEKLWDSHVVHERPGAPTLLFIDLHLVHEVTSPQAFQGLRERGLKVRRPDLTIAT
CCHHHHHHHHHCCCEECCCCCCEEEEEEEEEHHHCCCCHHHHHHHHCCCEEECCCEEEEE
ADHSIPTTDRSLPIVDEIAAKQLAQLETNCKDFGIRCLGVHSSQQGIVHVIGPELGLTQP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTATHGAFGALAFGIGTSEVEQVLASQCLLQRKSKTFQVKVDGALKAGVSA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCCCH
KDIILALIARIGIGGGTGSVFEYTGSAIRALSVEERMTVCNMSIEGGARAGLIAPDDATF
HHHHHHHHHHHCCCCCCCCCEEECCCHHEEEEHHHHHEEEEEEECCCCCCCEECCCCCCH
EYLAGRPHAPKGAAWDAAIPRWKQLPTDDGATYDRSVSIDADTLEPMITYGTNPGMGVSI
HHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCHHHCCCEEEECCCCCCCEEE
NAALPDPSQVSDPMARDSITKALAYMGLEGGKPLVGHPIDVVFIGSCTNSRISDLRTAAG
EECCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
LLKGRKVSPKVRVMVVPGSQEVKRQAIAEGLPEIFRAAGCDYREPGCSMCIAMNGDQLAP
HHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEECC
GEYSVSTSNRNFEGRQGKGGRTFLASPLTAAASAITGVVTDVRTLL
CCEEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PRTIIEKLWDSHVVHERPGAPTLLFIDLHLVHEVTSPQAFQGLRERGLKVRRPDLTIAT
CHHHHHHHHHCCCEECCCCCCEEEEEEEEEHHHCCCCHHHHHHHHCCCEEECCCEEEEE
ADHSIPTTDRSLPIVDEIAAKQLAQLETNCKDFGIRCLGVHSSQQGIVHVIGPELGLTQP
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTATHGAFGALAFGIGTSEVEQVLASQCLLQRKSKTFQVKVDGALKAGVSA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHCCCCH
KDIILALIARIGIGGGTGSVFEYTGSAIRALSVEERMTVCNMSIEGGARAGLIAPDDATF
HHHHHHHHHHHCCCCCCCCCEEECCCHHEEEEHHHHHEEEEEEECCCCCCCEECCCCCCH
EYLAGRPHAPKGAAWDAAIPRWKQLPTDDGATYDRSVSIDADTLEPMITYGTNPGMGVSI
HHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCHHHCCCEEEECCCCCCCEEE
NAALPDPSQVSDPMARDSITKALAYMGLEGGKPLVGHPIDVVFIGSCTNSRISDLRTAAG
EECCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
LLKGRKVSPKVRVMVVPGSQEVKRQAIAEGLPEIFRAAGCDYREPGCSMCIAMNGDQLAP
HHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEECC
GEYSVSTSNRNFEGRQGKGGRTFLASPLTAAASAITGVVTDVRTLL
CCEEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA