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The map label for this gene is lacZ [H]

Identifier: 116623148

GI number: 116623148

Start: 5143697

End: 5145979

Strand: Reverse

Name: lacZ [H]

Synonym: Acid_4054

Alternate gene names: 116623148

Gene position: 5145979-5143697 (Counterclockwise)

Preceding gene: 116623149

Following gene: 116623146

Centisome position: 51.64

GC content: 63.91

Gene sequence:

>2283_bases
ATGAAACTGGCCGTATGTCTGCTGTGGAGCGCCGCGCTTGCGGCCGCTCCGCGCCTGACCATTCCGTTCGACACGGACTG
GCGTTTTTCCCACGGCGACGAAACCGCGTCGCGAACGGTCACCCTGCCGCACGATTGGGGCATCGAAGGTCCCTTCGACG
AGAAGAACCCCAGCGGTTGGGCGGGCGCATTTCTGCCGGCCGGAGTGGGCTGGTACCGGAAGCAGTTCACTTTGCCAGCC
GGTACCGCGGGACGGCGCGTCTTCGTCGAGTTCGATGGCGTGATGGCCAATAGCGAGGTCTGGATCAATGATGTTTCGCT
GGGCAAGCGGCCGTATGGGTACGTGAGCTTCCGCTACGAGCTGACCGATCATCTCGCACCGGTCAACGTTCTCGCGGTTC
GCGCCGATAACTCGCGACAGCCGGCGTCGCGCTGGTTTCCGGGCGCCGGCATCAATCGCCACGTCCGCCTGATCGTTACC
GACCCGGTGCACCTCGATCAATGGGCTACGTTCGTCACTACACCGAAGGTGACCGCCGCGGAGGCAACCGTGAAATTCGC
GACGTCGGTGGTAAACCAGTCCGCCGCGCCGCACACCGTCAGCGTGGAATTCACCACTGCGGGCAAGACGGTGCAAACCA
AATCGCAGACCATCGCACCCGGCAAGTCTGCCGAGTTCGAGGCGGAGATCGCCGTGGCCAATCCCAAGTTGTGGGGCCTG
GAACCGCAGGAACTCTACGTCGCGACGGCCAGGGTGCGCGAGGGGAAGGACATGCTCGATGACGAGTCAGTTCCCTTCGG
CATTCGCGAAGCGCGGTTCGACGCCGATTCTGGCTTTTTGCTGAATGGCAAGAATCTGAAGATCAAAGGCGTCTGCCTGC
ACGCCGATGGCGGTGCAGTGGGTACCGCCGTCCCGCTCGATATCTGGGAGCGCCGGCTCGCCGCGCTCAGGAGGATCGGC
GTCAACGCGATCCGCACCGCCCACGGTCCGGTCGCGCCCGAGTTTCTCGATCTGACCGACCGCATGGGCTTCCTGGTGAT
GGACGAGATGTTCGATCAATGGACCGTTGCCAAAAATCTCTACGACTATCACCTCTTCTTCCGCGAGTGGTCCCACATCG
ACACGCGCGACACCGTGCACCGCGACCGCAACCACCCCAGCGTGATCATCTATAGCGCAGGCAATGAGATTCACGATACG
CCGCAGGCCGAACTCGCCAAGGGCATCCTCAAGGGATTGGTGGAGACCTTCCACACCGCCGACCCCACGCGTCCCGTCAC
GCAGGCGCTCTTCCGCCCCAACGTGAGCCACGATTACGATAACGGACTCGCCGACCTGCTCGACGTAGTCGGCCAGAATT
ACCGCGAGAACGAAATCCTCGCGGCCCATGCACAGAAGCCCACGCGCAAGATTCTCGGCACCGAGAATACACACGATCGC
CGCGCCTGGCTTGCCCTGCGCGATAGCCCTGAGTACGCCGGACAGTTCCTGTGGGCCGGCATCGATTACCTGGGCGAAGG
CCGCATGTGGCCTTACATCTCCAACTCCTTCGGCCTGCTCGACCGCACCGGCGCAATCAAGCCCATCGGCTGGGAGCGCG
CCAGTTGGTGGAGCACCGGGCCGGTGGTACACGCCACCCGCCGCGCCGCCGTGCGGGAATTGGCGCCCACCGATCCGGGC
TACGAGGCTGCCCGCCGCCAGACGCAGACGCTGTTTGACGATTGGTCGCCGGCCAATCGCGAACCGCACCCCGAGAACGT
AGAGGTGTACAGCAATTGCGAGGACGTGGAACTGCTGCTGAATGGGAAATCGCTCGGATCCAAGCCGCTTCCGAAGGATG
CCGCGCCCCGCAACTGGCACGTCGAGTTCGCCCCCGGAAAGATCGAGGCGCTGTGCCGCAATCAGGGCAAGGTGGCGGCC
GTGAGCGATCTGCGCACCGCCGGCAAGCCCGTCCGCATCGCGCTTTCCATCGACAAAGTGAAGCTGCCGTTCAACTGGGA
CAGCGTCGCCTACATCAACGCCAGCGTGGTCGATGAGAACGGCATCATGGTTCCCGCCGCAGCCGATAACATTGTGTTCG
CGATCGATGGCCCCGGTGCGATCGCCGCCGTGGACAATGCCGATACCACTAGCCACGAAGGCTTCCAGGCCAACGAGCGG
AGCGCGTTCCAGGGCCGCTGCATCGCCCTGGTGAAGGCATCGCGCGATCAGGGCCGCATCACCGTGAAAGCCTCGGCCGC
CGGTCTCGCTACCGGTTCCATCACGCTCGAGGCGGTCAAGTGA

Upstream 100 bases:

>100_bases
AGCCCAATGTGAGCTTCACGCCGGACCAGAAGTGGGTGGTCTTCCGATCCAACATGTTCGGCGCGACTTACGCGTTCGCC
GTCGAGGTCGAAAAGAGCAG

Downstream 100 bases:

>100_bases
GATTCGGTCCGGGTTTAGTGCGCGATCACCAGCGTCTCCGCCAGAAACTGCAGCACATCCTCGCGCCAGAGCCTGGCTCC
CGGCGCCGCAAACAGCAGAT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MKLAVCLLWSAALAAAPRLTIPFDTDWRFSHGDETASRTVTLPHDWGIEGPFDEKNPSGWAGAFLPAGVGWYRKQFTLPA
GTAGRRVFVEFDGVMANSEVWINDVSLGKRPYGYVSFRYELTDHLAPVNVLAVRADNSRQPASRWFPGAGINRHVRLIVT
DPVHLDQWATFVTTPKVTAAEATVKFATSVVNQSAAPHTVSVEFTTAGKTVQTKSQTIAPGKSAEFEAEIAVANPKLWGL
EPQELYVATARVREGKDMLDDESVPFGIREARFDADSGFLLNGKNLKIKGVCLHADGGAVGTAVPLDIWERRLAALRRIG
VNAIRTAHGPVAPEFLDLTDRMGFLVMDEMFDQWTVAKNLYDYHLFFREWSHIDTRDTVHRDRNHPSVIIYSAGNEIHDT
PQAELAKGILKGLVETFHTADPTRPVTQALFRPNVSHDYDNGLADLLDVVGQNYRENEILAAHAQKPTRKILGTENTHDR
RAWLALRDSPEYAGQFLWAGIDYLGEGRMWPYISNSFGLLDRTGAIKPIGWERASWWSTGPVVHATRRAAVRELAPTDPG
YEAARRQTQTLFDDWSPANREPHPENVEVYSNCEDVELLLNGKSLGSKPLPKDAAPRNWHVEFAPGKIEALCRNQGKVAA
VSDLRTAGKPVRIALSIDKVKLPFNWDSVAYINASVVDENGIMVPAAADNIVFAIDGPGAIAAVDNADTTSHEGFQANER
SAFQGRCIALVKASRDQGRITVKASAAGLATGSITLEAVK

Sequences:

>Translated_760_residues
MKLAVCLLWSAALAAAPRLTIPFDTDWRFSHGDETASRTVTLPHDWGIEGPFDEKNPSGWAGAFLPAGVGWYRKQFTLPA
GTAGRRVFVEFDGVMANSEVWINDVSLGKRPYGYVSFRYELTDHLAPVNVLAVRADNSRQPASRWFPGAGINRHVRLIVT
DPVHLDQWATFVTTPKVTAAEATVKFATSVVNQSAAPHTVSVEFTTAGKTVQTKSQTIAPGKSAEFEAEIAVANPKLWGL
EPQELYVATARVREGKDMLDDESVPFGIREARFDADSGFLLNGKNLKIKGVCLHADGGAVGTAVPLDIWERRLAALRRIG
VNAIRTAHGPVAPEFLDLTDRMGFLVMDEMFDQWTVAKNLYDYHLFFREWSHIDTRDTVHRDRNHPSVIIYSAGNEIHDT
PQAELAKGILKGLVETFHTADPTRPVTQALFRPNVSHDYDNGLADLLDVVGQNYRENEILAAHAQKPTRKILGTENTHDR
RAWLALRDSPEYAGQFLWAGIDYLGEGRMWPYISNSFGLLDRTGAIKPIGWERASWWSTGPVVHATRRAAVRELAPTDPG
YEAARRQTQTLFDDWSPANREPHPENVEVYSNCEDVELLLNGKSLGSKPLPKDAAPRNWHVEFAPGKIEALCRNQGKVAA
VSDLRTAGKPVRIALSIDKVKLPFNWDSVAYINASVVDENGIMVPAAADNIVFAIDGPGAIAAVDNADTTSHEGFQANER
SAFQGRCIALVKASRDQGRITVKASAAGLATGSITLEAVK
>Mature_760_residues
MKLAVCLLWSAALAAAPRLTIPFDTDWRFSHGDETASRTVTLPHDWGIEGPFDEKNPSGWAGAFLPAGVGWYRKQFTLPA
GTAGRRVFVEFDGVMANSEVWINDVSLGKRPYGYVSFRYELTDHLAPVNVLAVRADNSRQPASRWFPGAGINRHVRLIVT
DPVHLDQWATFVTTPKVTAAEATVKFATSVVNQSAAPHTVSVEFTTAGKTVQTKSQTIAPGKSAEFEAEIAVANPKLWGL
EPQELYVATARVREGKDMLDDESVPFGIREARFDADSGFLLNGKNLKIKGVCLHADGGAVGTAVPLDIWERRLAALRRIG
VNAIRTAHGPVAPEFLDLTDRMGFLVMDEMFDQWTVAKNLYDYHLFFREWSHIDTRDTVHRDRNHPSVIIYSAGNEIHDT
PQAELAKGILKGLVETFHTADPTRPVTQALFRPNVSHDYDNGLADLLDVVGQNYRENEILAAHAQKPTRKILGTENTHDR
RAWLALRDSPEYAGQFLWAGIDYLGEGRMWPYISNSFGLLDRTGAIKPIGWERASWWSTGPVVHATRRAAVRELAPTDPG
YEAARRQTQTLFDDWSPANREPHPENVEVYSNCEDVELLLNGKSLGSKPLPKDAAPRNWHVEFAPGKIEALCRNQGKVAA
VSDLRTAGKPVRIALSIDKVKLPFNWDSVAYINASVVDENGIMVPAAADNIVFAIDGPGAIAAVDNADTTSHEGFQANER
SAFQGRCIALVKASRDQGRITVKASAAGLATGSITLEAVK

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=429, Percent_Identity=25.6410256410256, Blast_Score=99, Evalue=2e-20,
Organism=Escherichia coli, GI1787903, Length=425, Percent_Identity=27.7647058823529, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI48994920, Length=382, Percent_Identity=27.7486910994764, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1786539, Length=649, Percent_Identity=25.115562403698, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17510825, Length=425, Percent_Identity=25.6470588235294, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24651745, Length=424, Percent_Identity=25, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24655438, Length=401, Percent_Identity=26.4339152119701, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922582, Length=401, Percent_Identity=26.4339152119701, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI62471735, Length=401, Percent_Identity=26.4339152119701, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 83561; Mature: 83561

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVCLLWSAALAAAPRLTIPFDTDWRFSHGDETASRTVTLPHDWGIEGPFDEKNPSGW
CEEEEEEHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
AGAFLPAGVGWYRKQFTLPAGTAGRRVFVEFDGVMANSEVWINDVSLGKRPYGYVSFRYE
CCCHHHCCCCHHHEEEECCCCCCCCEEEEEECCEEECCEEEEEECCCCCCCCEEEEEEEE
LTDHLAPVNVLAVRADNSRQPASRWFPGAGINRHVRLIVTDPVHLDQWATFVTTPKVTAA
ECCCCCCEEEEEEEECCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCHH
EATVKFATSVVNQSAAPHTVSVEFTTAGKTVQTKSQTIAPGKSAEFEAEIAVANPKLWGL
HHHHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCEECCCCCCCEEEEEEEECCCEECC
EPQELYVATARVREGKDMLDDESVPFGIREARFDADSGFLLNGKNLKIKGVCLHADGGAV
CHHHEEEEEHHHHCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCEEEEEEEEECCCCCC
GTAVPLDIWERRLAALRRIGVNAIRTAHGPVAPEFLDLTDRMGFLVMDEMFDQWTVAKNL
CCCCCHHHHHHHHHHHHHHCHHHEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
YDYHLFFREWSHIDTRDTVHRDRNHPSVIIYSAGNEIHDTPQAELAKGILKGLVETFHTA
HHHHHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
DPTRPVTQALFRPNVSHDYDNGLADLLDVVGQNYRENEILAAHAQKPTRKILGTENTHDR
CCCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHCCCCCCCCC
RAWLALRDSPEYAGQFLWAGIDYLGEGRMWPYISNSFGLLDRTGAIKPIGWERASWWSTG
EEEEEEECCHHHHCEEEEECHHHCCCCCCCCEECCCCCCEECCCCCCCCCCCCCCCCCCC
PVVHATRRAAVRELAPTDPGYEAARRQTQTLFDDWSPANREPHPENVEVYSNCEDVELLL
CEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEE
NGKSLGSKPLPKDAAPRNWHVEFAPGKIEALCRNQGKVAAVSDLRTAGKPVRIALSIDKV
ECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCEEEEHHHHHCCCCEEEEEEEEEE
KLPFNWDSVAYINASVVDENGIMVPAAADNIVFAIDGPGAIAAVDNADTTSHEGFQANER
ECCCCCCCEEEEEEEEECCCCEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCC
SAFQGRCIALVKASRDQGRITVKASAAGLATGSITLEAVK
CCCCCEEEEEEEECCCCCEEEEEECCCCEEECEEEEEEEC
>Mature Secondary Structure
MKLAVCLLWSAALAAAPRLTIPFDTDWRFSHGDETASRTVTLPHDWGIEGPFDEKNPSGW
CEEEEEEHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
AGAFLPAGVGWYRKQFTLPAGTAGRRVFVEFDGVMANSEVWINDVSLGKRPYGYVSFRYE
CCCHHHCCCCHHHEEEECCCCCCCCEEEEEECCEEECCEEEEEECCCCCCCCEEEEEEEE
LTDHLAPVNVLAVRADNSRQPASRWFPGAGINRHVRLIVTDPVHLDQWATFVTTPKVTAA
ECCCCCCEEEEEEEECCCCCCHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCHH
EATVKFATSVVNQSAAPHTVSVEFTTAGKTVQTKSQTIAPGKSAEFEAEIAVANPKLWGL
HHHHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCEECCCCCCCEEEEEEEECCCEECC
EPQELYVATARVREGKDMLDDESVPFGIREARFDADSGFLLNGKNLKIKGVCLHADGGAV
CHHHEEEEEHHHHCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCEEEEEEEEECCCCCC
GTAVPLDIWERRLAALRRIGVNAIRTAHGPVAPEFLDLTDRMGFLVMDEMFDQWTVAKNL
CCCCCHHHHHHHHHHHHHHCHHHEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
YDYHLFFREWSHIDTRDTVHRDRNHPSVIIYSAGNEIHDTPQAELAKGILKGLVETFHTA
HHHHHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
DPTRPVTQALFRPNVSHDYDNGLADLLDVVGQNYRENEILAAHAQKPTRKILGTENTHDR
CCCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHCCCCCCCCC
RAWLALRDSPEYAGQFLWAGIDYLGEGRMWPYISNSFGLLDRTGAIKPIGWERASWWSTG
EEEEEEECCHHHHCEEEEECHHHCCCCCCCCEECCCCCCEECCCCCCCCCCCCCCCCCCC
PVVHATRRAAVRELAPTDPGYEAARRQTQTLFDDWSPANREPHPENVEVYSNCEDVELLL
CEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEE
NGKSLGSKPLPKDAAPRNWHVEFAPGKIEALCRNQGKVAAVSDLRTAGKPVRIALSIDKV
ECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCEEEEHHHHHCCCCEEEEEEEEEE
KLPFNWDSVAYINASVVDENGIMVPAAADNIVFAIDGPGAIAAVDNADTTSHEGFQANER
ECCCCCCCEEEEEEEEECCCCEEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCC
SAFQGRCIALVKASRDQGRITVKASAAGLATGSITLEAVK
CCCCCEEEEEEEECCCCCEEEEEECCCCEEECEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA