Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 116622949

GI number: 116622949

Start: 4886291

End: 4886851

Strand: Reverse

Name: hisH [H]

Synonym: Acid_3851

Alternate gene names: 116622949

Gene position: 4886851-4886291 (Counterclockwise)

Preceding gene: 116622950

Following gene: 116622948

Centisome position: 49.04

GC content: 61.32

Gene sequence:

>561_bases
ATGATCACCATCCTCGATTACGGCGCCGGTAACCTGCGCAGCGTGCAAAACACGTTGGCCGAATTGAACTGCGAGTACCG
CCTGGTCAACGACGCTGCCGGCCTGCAATCTGCAACCAAAATTCTCCTCCCCGGTGTCGGCCACTTCGGCCAGATGATGC
ACGCGCTCGATACCCTGAGCGTGCGCGAAGCGCTGGTCGATCGTATTCGCGCCAGCGTCCCGTTCTTCGGCATCTGTCTC
GGCATGCAGGCGCTCTTCGAATCCAGCGAAGAGGCCCCCGAGGTCCGCGGCCTCGGCCTTTACCCCGGCATAGTCCGCCG
CTTCCCGATGGATGCGCGTGTTCCCCACATGGGTTGGAATCAGCTGGAACCCGGCGGCCGCTACGTCTACTTCGCGCACA
GTTACTATGTCCCCGAAAATCAGTACACCTCCGCCGCCTGCACTTACGGCGTTCGCTTCACCGCCATGCTGCAAGCGGGC
AGCGTACAGGGCGTCCAGTTCCACCCCGAAAAATCCAGCACGGTCGGCCTCCAGATCGTGAAAGGCTTCCTCGAATGTTA
A

Upstream 100 bases:

>100_bases
AGCTGGAAGCCATCTTCAAATGCTTCGCGCGTGCCATGAAATACGCCTGCTCCAAAGATGCACGCCTCAAAGATCAACTG
CCCTCCACCAAGGGATTGCT

Downstream 100 bases:

>100_bases
TGTGTTCGCCGGCGGGCGCCTTCAATCCGAGCCGCGAGCGTCAGGGAGCGTCCTCATGCTAGCCAAGCGCATCATCCCGT
GCCTCGACGTTACCGGCGGC

Product: imidazole glycerol phosphate synthase subunit hisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MITILDYGAGNLRSVQNTLAELNCEYRLVNDAAGLQSATKILLPGVGHFGQMMHALDTLSVREALVDRIRASVPFFGICL
GMQALFESSEEAPEVRGLGLYPGIVRRFPMDARVPHMGWNQLEPGGRYVYFAHSYYVPENQYTSAACTYGVRFTAMLQAG
SVQGVQFHPEKSSTVGLQIVKGFLEC

Sequences:

>Translated_186_residues
MITILDYGAGNLRSVQNTLAELNCEYRLVNDAAGLQSATKILLPGVGHFGQMMHALDTLSVREALVDRIRASVPFFGICL
GMQALFESSEEAPEVRGLGLYPGIVRRFPMDARVPHMGWNQLEPGGRYVYFAHSYYVPENQYTSAACTYGVRFTAMLQAG
SVQGVQFHPEKSSTVGLQIVKGFLEC
>Mature_186_residues
MITILDYGAGNLRSVQNTLAELNCEYRLVNDAAGLQSATKILLPGVGHFGQMMHALDTLSVREALVDRIRASVPFFGICL
GMQALFESSEEAPEVRGLGLYPGIVRRFPMDARVPHMGWNQLEPGGRYVYFAHSYYVPENQYTSAACTYGVRFTAMLQAG
SVQGVQFHPEKSSTVGLQIVKGFLEC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=40.6091370558376, Blast_Score=127, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=30.1435406698565, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 20469; Mature: 20469

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITILDYGAGNLRSVQNTLAELNCEYRLVNDAAGLQSATKILLPGVGHFGQMMHALDTLS
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHCHHHHHHHHCCCCCHHHHHHHHHHHHH
VREALVDRIRASVPFFGICLGMQALFESSEEAPEVRGLGLYPGIVRRFPMDARVPHMGWN
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCC
QLEPGGRYVYFAHSYYVPENQYTSAACTYGVRFTAMLQAGSVQGVQFHPEKSSTVGLQIV
CCCCCCCEEEEEEEEECCCCCCCCHHHHHCHHEEEEEECCCCCCEEECCCCCCHHHHHHH
KGFLEC
HHHHCC
>Mature Secondary Structure
MITILDYGAGNLRSVQNTLAELNCEYRLVNDAAGLQSATKILLPGVGHFGQMMHALDTLS
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHCHHHHHHHHCCCCCHHHHHHHHHHHHH
VREALVDRIRASVPFFGICLGMQALFESSEEAPEVRGLGLYPGIVRRFPMDARVPHMGWN
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCC
QLEPGGRYVYFAHSYYVPENQYTSAACTYGVRFTAMLQAGSVQGVQFHPEKSSTVGLQIV
CCCCCCCEEEEEEEEECCCCCCCCHHHHHCHHEEEEEECCCCCCEEECCCCCCHHHHHHH
KGFLEC
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]