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The map label for this gene is camA [H]

Identifier: 116622785

GI number: 116622785

Start: 4676729

End: 4677946

Strand: Direct

Name: camA [H]

Synonym: Acid_3685

Alternate gene names: 116622785

Gene position: 4676729-4677946 (Clockwise)

Preceding gene: 116622784

Following gene: 116622786

Centisome position: 46.93

GC content: 59.77

Gene sequence:

>1218_bases
ATGGCCAGCTTTAAGCACCTTATTTTAGGTGGAGGAATGGTCGCGGGCCACTGCGCCAAGCAGTACGTGGAGAACGGCGG
CAAAGCCGGAGAACTCGCGATTGTGTCCGGCGACGATGCGCTGCCCTACGAGCGCCCGCCGCTCTCCAAAGGCTTTCTCG
CCGGCAAAGACAGCGAGGAGTCCGTTCGGATCAACGCCGCGGAATTCTACGCCGAGCATGGAATCGACGTGAGGTTGAAT
ACCCGCGTCAATTCGATCGATGCGCGCGGCGGACGTCTGTCCACAAGCTCGGGCGAAGAATTCGGTTTTGAGAAACTGAT
CCTCGCCACCGGAGCGGAAGTGCGGCGGCTCGACGTGCCCGGCGCCGCTTCCAGCAATGTCCTGTACCTGCGCTCTTTGA
ACGATTCGAAACGGCTACGCGACGCCTCGATAAAGGCCAAACATGCCGTGGTTGCGGGCGGCGGATTTATCGCGATGGAA
GTTGCTTCCGTGCTGGCGAGCCGTGGAATCGAGACCACCATACTGGCGCGCCAAAACCGTTTCGGCGCCGCGTTTTTCAC
GCCCGAGATGTCCGCATTCTTCGAAAAGTATTACGTCGATCGAGGTGTGCGAATCCTCAAACAGACCGAAGTAATGGGCA
TCGAGAAGGGATCGAGAGCACTGCTGAAAGACGGGCGGGCAGTCGATTTCGATCTCTTCCTTGCGGGTATCGGAGTGCAG
CCGGTGACAGTACTGGCCGAGAAAGCCGGCTTGCCGGTGGACAACGGCATTCTGGTCAACGAGTATCTCGAAACCCGTGA
TGCGAACCTGTACGCCGCGGGCGACGTAGCCAACTATCCGGATTCACTGTTCGGCATGAAACGGCGGCGGGTAGAACACT
GGGACAACGCCGTCTCGCAAGGCCAGTACCTGGCCGGCGCCCTGCTTGGCAAACGCGAACCGTTCGTTCACGTGCCGTAT
TTCTTCTCGGATGTCTTCGACCTTTCGTACGAATTCTGGGGCGATCCCTCGCCGTCCGACCGCGTTGTGCACCGTGGCGA
TCTACAAACCAGCAGCTTCAGCATCTGGTGGCTGTCACAAAACCGGCTGGTGGCCGCCTTCGCGATGAACCGTCCCGATG
AGGAACGTGAACTGGCTCCGGAGCTGATTCGCTCTAGGAAGCTTCTCTCGGCCGAGCGATTACGTGAGGCAGGGTCCGTG
CGTGACGCGGCCGCATGA

Upstream 100 bases:

>100_bases
GTATCTCTGCGGACTGCACGGTTTCGACATTGTATCGACGGACGCAAGGCGCGCGCGGCGAACGGCGCGATGTGCCGCTC
GGCGGGAGTACGATGGCACT

Downstream 100 bases:

>100_bases
TGCGGGTCGCACCGCGTCAGTTATAAGATTGGCGCATGGATCTTACTGTCCACTTCCAACTGCTCGCGCGTTACAACCGT
ATCGCAAACGAGCGGCTATA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MASFKHLILGGGMVAGHCAKQYVENGGKAGELAIVSGDDALPYERPPLSKGFLAGKDSEESVRINAAEFYAEHGIDVRLN
TRVNSIDARGGRLSTSSGEEFGFEKLILATGAEVRRLDVPGAASSNVLYLRSLNDSKRLRDASIKAKHAVVAGGGFIAME
VASVLASRGIETTILARQNRFGAAFFTPEMSAFFEKYYVDRGVRILKQTEVMGIEKGSRALLKDGRAVDFDLFLAGIGVQ
PVTVLAEKAGLPVDNGILVNEYLETRDANLYAAGDVANYPDSLFGMKRRRVEHWDNAVSQGQYLAGALLGKREPFVHVPY
FFSDVFDLSYEFWGDPSPSDRVVHRGDLQTSSFSIWWLSQNRLVAAFAMNRPDEERELAPELIRSRKLLSAERLREAGSV
RDAAA

Sequences:

>Translated_405_residues
MASFKHLILGGGMVAGHCAKQYVENGGKAGELAIVSGDDALPYERPPLSKGFLAGKDSEESVRINAAEFYAEHGIDVRLN
TRVNSIDARGGRLSTSSGEEFGFEKLILATGAEVRRLDVPGAASSNVLYLRSLNDSKRLRDASIKAKHAVVAGGGFIAME
VASVLASRGIETTILARQNRFGAAFFTPEMSAFFEKYYVDRGVRILKQTEVMGIEKGSRALLKDGRAVDFDLFLAGIGVQ
PVTVLAEKAGLPVDNGILVNEYLETRDANLYAAGDVANYPDSLFGMKRRRVEHWDNAVSQGQYLAGALLGKREPFVHVPY
FFSDVFDLSYEFWGDPSPSDRVVHRGDLQTSSFSIWWLSQNRLVAAFAMNRPDEERELAPELIRSRKLLSAERLREAGSV
RDAAA
>Mature_404_residues
ASFKHLILGGGMVAGHCAKQYVENGGKAGELAIVSGDDALPYERPPLSKGFLAGKDSEESVRINAAEFYAEHGIDVRLNT
RVNSIDARGGRLSTSSGEEFGFEKLILATGAEVRRLDVPGAASSNVLYLRSLNDSKRLRDASIKAKHAVVAGGGFIAMEV
ASVLASRGIETTILARQNRFGAAFFTPEMSAFFEKYYVDRGVRILKQTEVMGIEKGSRALLKDGRAVDFDLFLAGIGVQP
VTVLAEKAGLPVDNGILVNEYLETRDANLYAAGDVANYPDSLFGMKRRRVEHWDNAVSQGQYLAGALLGKREPFVHVPYF
FSDVFDLSYEFWGDPSPSDRVVHRGDLQTSSFSIWWLSQNRLVAAFAMNRPDEERELAPELIRSRKLLSAERLREAGSVR
DAAA

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=370, Percent_Identity=26.4864864864865, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI226437568, Length=370, Percent_Identity=26.4864864864865, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI21389617, Length=370, Percent_Identity=26.4864864864865, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4757732, Length=339, Percent_Identity=30.9734513274336, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI22202629, Length=339, Percent_Identity=30.9734513274336, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI195927004, Length=140, Percent_Identity=37.1428571428571, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI22202631, Length=140, Percent_Identity=37.1428571428571, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1788892, Length=395, Percent_Identity=29.873417721519, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789065, Length=272, Percent_Identity=29.4117647058824, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789765, Length=298, Percent_Identity=23.8255033557047, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=371, Percent_Identity=30.4582210242588, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24639257, Length=403, Percent_Identity=27.5434243176179, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281359715, Length=403, Percent_Identity=27.5434243176179, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281359713, Length=403, Percent_Identity=27.5434243176179, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24639250, Length=403, Percent_Identity=27.5434243176179, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18543267, Length=403, Percent_Identity=27.5434243176179, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24639252, Length=403, Percent_Identity=27.5434243176179, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24585130, Length=388, Percent_Identity=25.2577319587629, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24581020, Length=367, Percent_Identity=27.7929155313352, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI28573993, Length=367, Percent_Identity=27.7929155313352, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 44344; Mature: 44212

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFKHLILGGGMVAGHCAKQYVENGGKAGELAIVSGDDALPYERPPLSKGFLAGKDSEE
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
SVRINAAEFYAEHGIDVRLNTRVNSIDARGGRLSTSSGEEFGFEKLILATGAEVRRLDVP
CEEEEHHHHHHHCCCEEEEECCCCEECCCCCEECCCCCCCCCHHEEEEECCCCEEEEECC
GAASSNVLYLRSLNDSKRLRDASIKAKHAVVAGGGFIAMEVASVLASRGIETTILARQNR
CCCCCCEEEEEECCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCC
FGAAFFTPEMSAFFEKYYVDRGVRILKQTEVMGIEKGSRALLKDGRAVDFDLFLAGIGVQ
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEEEEECCCCC
PVTVLAEKAGLPVDNGILVNEYLETRDANLYAAGDVANYPDSLFGMKRRRVEHWDNAVSQ
HHHHHHHHCCCCCCCCEEEEHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GQYLAGALLGKREPFVHVPYFFSDVFDLSYEFWGDPSPSDRVVHRGDLQTSSFSIWWLSQ
CHHHHHHHHCCCCCEEECCHHHHHHHHCCHHCCCCCCCCCCEEECCCCCCCCEEEEEEEC
NRLVAAFAMNRPDEERELAPELIRSRKLLSAERLREAGSVRDAAA
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ASFKHLILGGGMVAGHCAKQYVENGGKAGELAIVSGDDALPYERPPLSKGFLAGKDSEE
CCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
SVRINAAEFYAEHGIDVRLNTRVNSIDARGGRLSTSSGEEFGFEKLILATGAEVRRLDVP
CEEEEHHHHHHHCCCEEEEECCCCEECCCCCEECCCCCCCCCHHEEEEECCCCEEEEECC
GAASSNVLYLRSLNDSKRLRDASIKAKHAVVAGGGFIAMEVASVLASRGIETTILARQNR
CCCCCCEEEEEECCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCC
FGAAFFTPEMSAFFEKYYVDRGVRILKQTEVMGIEKGSRALLKDGRAVDFDLFLAGIGVQ
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEEEEECCCCC
PVTVLAEKAGLPVDNGILVNEYLETRDANLYAAGDVANYPDSLFGMKRRRVEHWDNAVSQ
HHHHHHHHCCCCCCCCEEEEHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GQYLAGALLGKREPFVHVPYFFSDVFDLSYEFWGDPSPSDRVVHRGDLQTSSFSIWWLSQ
CHHHHHHHHCCCCCEEECCHHHHHHHHCCHHCCCCCCCCCCEEECCCCCCCCEEEEEEEC
NRLVAAFAMNRPDEERELAPELIRSRKLLSAERLREAGSVRDAAA
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]