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The map label for this gene is cysS

Identifier: 116622355

GI number: 116622355

Start: 4112463

End: 4113863

Strand: Direct

Name: cysS

Synonym: Acid_3249

Alternate gene names: 116622355

Gene position: 4112463-4113863 (Clockwise)

Preceding gene: 116622354

Following gene: 116622356

Centisome position: 41.27

GC content: 60.67

Gene sequence:

>1401_bases
ATGGCGCTGCGCTTCTACAACACGTTGTCCCAACAGGTCGAGGAGTTTACCCCGGCGAGCGACAACACCGTCCGCATGTA
CACCTGCGGCCCCACGGTCTACGATTTTGCCCACATCGGCAATTTCCGGACCTTCACCTTCGTGGACCTTCTGCGCAAGG
TGCTCCGCGCCAACGGCTTCCAACTCAACCACGTCATGAACATTACCGACGTGGACGACAAAATCATCCGCAATGCCGTG
GCCCAGCACCAGTCGCTCGAGCAATACACGAAGATCTACACGGAGGCCTTCCTCGAGGATTGCAAAATGCTGCGCCTGGA
GCGCCCCGAGCGCATCGCTCCCGCCACCGAGCATATCCACGACATGGTCTCCGCGATCGAGCGCCTCGGCGATTCGCAGC
ACACCTATGCCAGCGACGGCTCGGTCTATTTCAAGATCGCCAGCTTCCCCGGGTATGGCAAGCTCTCGCACAACGATTTC
TCCGGTAACCTCGCCGGCGCTCGCGTGGATGTCGATGAATATGAAAAAGCCGACGCCCGCGATTTCGCGCTCTGGAAGAC
TCCCAAAGAGGGCGAGCCTTTCTGGGATACCACCATCGGCCCCGGGCGCCCCGGCTGGCACATCGAATGCTCCGTGATGG
CGATCAAGTATCTCGGCGAGACTCTCGATATCCACGCCGGCGGGATCGACCTCGTATTTCCGCATCACGAAAACGAGATC
GCGCAATCCGAATCGCTGACCGGCAAACTCTTCTCGCGCTTCTGGCTTCACGCCGAGTTCCTGATGGTGGAAGGCCAGAA
GATGTCGAAGTCGCTGGGCAACTACTACACGCTGCGCGACCTGGTCGCCAAAGGCTACGAGCCGGAGTCCATCCGCTACC
TGCTTGCCTCCGTCCCCTACCGCAAGAGCCTCAACTTCACGCTCGATGGACTCAAGGCCGCGCGCATCGCGATCGAGCGC
CTGCGCAATTTCAAGCGCCGCCTGGAAACCGAACCCTTCGCCGAAGGCCTCAGCGCCATTCTCGCCGAACGCACCGCACA
GGCATCGCAGGCGTTCATCGGTGGCCTCAATGAGGATCTCAATACCGCCGAGGCCCTGGCTTCCGTTTTCGAATACGTAC
GCGATCTCAACAGCGCCATGGACAGCGGCGAGTTTCGCGCAGGCAACACTACCGCGGCGCTGGAATTCCTGGCGCGCTTC
GATTCCATCTTCGACGTGCTGAAGCCCACCGCCAAAGCCGGCGAGCTTTCCGACGCCGAAATCGATGCTCAAATCGCGGC
CCGCGCCGCCGCCAAAAAATCGCGCGATTTCGCGCTTGCCGATCAAATTCGCGACCAGCTCCTGGCCCAGGGCATCATCC
TCGAGGACACCAAGAGCGGGGTTCGCTGGAAAAGGAAATAG

Upstream 100 bases:

>100_bases
GAATGCCGTCGCCGCGCGCCCCGTAACCGATTTGGGCTGACCTCTCGCGGGTGAACAATCACGCGCACGCGTCTGTTACG
ATGAAATTTCCCATCACATT

Downstream 100 bases:

>100_bases
GTTTCAGGTTTGAAGATCCATACCCAGGCAGTTCACACAGGCGACCGCCGCAAGCCGGGCGCCCATACCCCGGTTACCAC
CCCGATCCACCTCGCCACCA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MALRFYNTLSQQVEEFTPASDNTVRMYTCGPTVYDFAHIGNFRTFTFVDLLRKVLRANGFQLNHVMNITDVDDKIIRNAV
AQHQSLEQYTKIYTEAFLEDCKMLRLERPERIAPATEHIHDMVSAIERLGDSQHTYASDGSVYFKIASFPGYGKLSHNDF
SGNLAGARVDVDEYEKADARDFALWKTPKEGEPFWDTTIGPGRPGWHIECSVMAIKYLGETLDIHAGGIDLVFPHHENEI
AQSESLTGKLFSRFWLHAEFLMVEGQKMSKSLGNYYTLRDLVAKGYEPESIRYLLASVPYRKSLNFTLDGLKAARIAIER
LRNFKRRLETEPFAEGLSAILAERTAQASQAFIGGLNEDLNTAEALASVFEYVRDLNSAMDSGEFRAGNTTAALEFLARF
DSIFDVLKPTAKAGELSDAEIDAQIAARAAAKKSRDFALADQIRDQLLAQGIILEDTKSGVRWKRK

Sequences:

>Translated_466_residues
MALRFYNTLSQQVEEFTPASDNTVRMYTCGPTVYDFAHIGNFRTFTFVDLLRKVLRANGFQLNHVMNITDVDDKIIRNAV
AQHQSLEQYTKIYTEAFLEDCKMLRLERPERIAPATEHIHDMVSAIERLGDSQHTYASDGSVYFKIASFPGYGKLSHNDF
SGNLAGARVDVDEYEKADARDFALWKTPKEGEPFWDTTIGPGRPGWHIECSVMAIKYLGETLDIHAGGIDLVFPHHENEI
AQSESLTGKLFSRFWLHAEFLMVEGQKMSKSLGNYYTLRDLVAKGYEPESIRYLLASVPYRKSLNFTLDGLKAARIAIER
LRNFKRRLETEPFAEGLSAILAERTAQASQAFIGGLNEDLNTAEALASVFEYVRDLNSAMDSGEFRAGNTTAALEFLARF
DSIFDVLKPTAKAGELSDAEIDAQIAARAAAKKSRDFALADQIRDQLLAQGIILEDTKSGVRWKRK
>Mature_465_residues
ALRFYNTLSQQVEEFTPASDNTVRMYTCGPTVYDFAHIGNFRTFTFVDLLRKVLRANGFQLNHVMNITDVDDKIIRNAVA
QHQSLEQYTKIYTEAFLEDCKMLRLERPERIAPATEHIHDMVSAIERLGDSQHTYASDGSVYFKIASFPGYGKLSHNDFS
GNLAGARVDVDEYEKADARDFALWKTPKEGEPFWDTTIGPGRPGWHIECSVMAIKYLGETLDIHAGGIDLVFPHHENEIA
QSESLTGKLFSRFWLHAEFLMVEGQKMSKSLGNYYTLRDLVAKGYEPESIRYLLASVPYRKSLNFTLDGLKAARIAIERL
RNFKRRLETEPFAEGLSAILAERTAQASQAFIGGLNEDLNTAEALASVFEYVRDLNSAMDSGEFRAGNTTAALEFLARFD
SIFDVLKPTAKAGELSDAEIDAQIAARAAAKKSRDFALADQIRDQLLAQGIILEDTKSGVRWKRK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=491, Percent_Identity=32.9938900203666, Blast_Score=220, Evalue=3e-57,
Organism=Homo sapiens, GI21269877, Length=260, Percent_Identity=33.4615384615385, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI62240992, Length=260, Percent_Identity=33.4615384615385, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI303304987, Length=260, Percent_Identity=33.4615384615385, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI10835051, Length=260, Percent_Identity=33.4615384615385, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1786737, Length=474, Percent_Identity=41.3502109704641, Blast_Score=326, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17509797, Length=263, Percent_Identity=34.2205323193916, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17509795, Length=263, Percent_Identity=34.2205323193916, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6324082, Length=333, Percent_Identity=32.1321321321321, Blast_Score=170, Evalue=4e-43,
Organism=Drosophila melanogaster, GI19922218, Length=503, Percent_Identity=31.2127236580517, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI19922364, Length=450, Percent_Identity=29.3333333333333, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_SOLUE (Q021Y7)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_824511.1
- ProteinModelPortal:   Q021Y7
- SMR:   Q021Y7
- STRING:   Q021Y7
- GeneID:   4429019
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_3249
- NMPDR:   fig|234267.9.peg.3084
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52430; Mature: 52299

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRFYNTLSQQVEEFTPASDNTVRMYTCGPTVYDFAHIGNFRTFTFVDLLRKVLRANGF
CCEEHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCE
QLNHVMNITDVDDKIIRNAVAQHQSLEQYTKIYTEAFLEDCKMLRLERPERIAPATEHIH
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
DMVSAIERLGDSQHTYASDGSVYFKIASFPGYGKLSHNDFSGNLAGARVDVDEYEKADAR
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
DFALWKTPKEGEPFWDTTIGPGRPGWHIECSVMAIKYLGETLDIHAGGIDLVFPHHENEI
CEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEEECCCEEEEECCCCCHH
AQSESLTGKLFSRFWLHAEFLMVEGQKMSKSLGNYYTLRDLVAKGYEPESIRYLLASVPY
HHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCC
RKSLNFTLDGLKAARIAIERLRNFKRRLETEPFAEGLSAILAERTAQASQAFIGGLNEDL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTAEALASVFEYVRDLNSAMDSGEFRAGNTTAALEFLARFDSIFDVLKPTAKAGELSDAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
IDAQIAARAAAKKSRDFALADQIRDQLLAQGIILEDTKSGVRWKRK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
ALRFYNTLSQQVEEFTPASDNTVRMYTCGPTVYDFAHIGNFRTFTFVDLLRKVLRANGF
CEEHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCE
QLNHVMNITDVDDKIIRNAVAQHQSLEQYTKIYTEAFLEDCKMLRLERPERIAPATEHIH
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
DMVSAIERLGDSQHTYASDGSVYFKIASFPGYGKLSHNDFSGNLAGARVDVDEYEKADAR
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
DFALWKTPKEGEPFWDTTIGPGRPGWHIECSVMAIKYLGETLDIHAGGIDLVFPHHENEI
CEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEEECCCEEEEECCCCCHH
AQSESLTGKLFSRFWLHAEFLMVEGQKMSKSLGNYYTLRDLVAKGYEPESIRYLLASVPY
HHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCC
RKSLNFTLDGLKAARIAIERLRNFKRRLETEPFAEGLSAILAERTAQASQAFIGGLNEDL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTAEALASVFEYVRDLNSAMDSGEFRAGNTTAALEFLARFDSIFDVLKPTAKAGELSDAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
IDAQIAARAAAKKSRDFALADQIRDQLLAQGIILEDTKSGVRWKRK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA