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The map label for this gene is liaS [H]

Identifier: 116621907

GI number: 116621907

Start: 3537485

End: 3540001

Strand: Direct

Name: liaS [H]

Synonym: Acid_2792

Alternate gene names: 116621907

Gene position: 3537485-3540001 (Clockwise)

Preceding gene: 116621901

Following gene: 116621908

Centisome position: 35.5

GC content: 61.54

Gene sequence:

>2517_bases
ATGAGTGACGAGGGAGCTGTCAAGCCGGATTTCGAGAGGCTGTTCAACTCAGTGACAGGGGACTATCTGGTGGTTGATGC
CAATCTGCGGGTAGTCGCGGCCAGCGACGGTTACCTCCAAGAAGCCATGGTGAGCCGAACCGACATACTCGGGCTGGACG
TTTTCGAGGCGTTTCGAGGGAAGCGCCACAGTGAGCACACGGTCCGAATGGAGCCGGAATATCCGCAGCCGCTCGAGCCA
GACGAACTGAGCGGGAGGCTCTCCGTGGCGAACGACGAACTGGTCAAGCGCGCTGAGGAGCTTCAAGCCGCGCTGGACGC
CGCGCCGGTCGCCATGTGGATTGCCCACGACCCGAAATGTTTGCGCATCACCGGAAATGCTTACGCGGACAAGATCCTGA
TGGGGGCCGAGTGGGGAGGCGATAGATCAGCCAGCGCGCAGTCTCGCGATGTGGCAGTCTCGTATCGGGCTTTTCGCAAC
GGCATGGAACTGCTGCCAGAGGACCTGCCTGCCCAGGTCGCGGCAGCCGCGGGCAAGCCGGTGCCCGAAGGAGAAATCGA
GCTGGTCTTCTCCGATCGCCGAACCGTCAATATGCTGATGAGCGCCGTGCCTCTGTACAACGCGGAGGGCCAGGTTCGGG
GCTCGGTTGCGGTGGGGGTGGACGTGACCGCCCGAAAGCGCGCGGAAGCGGCGCTGCGCGACAGCGAGGAGAAGTTTCGA
ACTCTGGCGAACGCCATCCCGCAGTTGTGCTCGATGGCAAATGCCGATGGCTGGATTTCGTGGTACAACCAGCGCTGGTA
CGAGTATACGGGGACAACACCCGAACAGATGGAGGGGTGGGGCTGGCAATCGGTTCACGATCCCGACACGCTGGCGGAGG
TGCTTGACCGGTGGAAGCATTCGATTTCGAGCGGAGAGCCCTTCGACATGACGTTCCCGCTGCGGGGCGCCGATGGTGTG
TTTCGCCCTTTCCTGACACGAATCATGGCCGTCCGCGATCGGGATGGCATGGTGACCGGGTGGTTCGGGACAAACACGGA
TGTCAGCGAACAGCAGAAGATTGAGGAGGCGCTGCGCGGAAGCGAGGAACTGTTGAAGACCTTCGTCAGGCACGTGCCGG
CAGCAGTAGCGATGCTCGATCGCGAAATGCGATACCTCCAGGTGAGTGACCGCTGGTATGCCGATTACTCCCTGATAAAC
GGTGAGATCCTGGGGCGGTCGCACTACGAGGTCTTTCCGGACATTCCAGAGCATTGGAAGCAACTTCACAGCCGCTGCCT
CGCCGGAGAAACGCTGCGCGCAGACGAGGACTGTTGGGAGCACGCCGACGGAGAGACGACGTGGCTCCGCTGGGAAATAC
GGCCGTGGGGTGATCAGACAGAGAAATCCGCAGGGAAGCCGGCAGGCGTCCTGATTTTTACCGAAGACATCACAGAGCGA
AAAAGGATTGAGGCATCGCTGCGTGAGCGTGAGGCGACCATTCGCGCTCTTCTGGAAACCGCGGCCCAATCGATATTGGC
GGTGGACGCGAAAGGCGTTGTAGTTCTGGCCAACCGGATGGCTGAGGAGATGTTCGGCTTCGGACCGAGCGAACTTCCGG
GAATGCGGCTCGAGCAGTTGCTACCGGAGCGTAGCCGCGAAGGGCACGTTTTCTACCGTGCCGAATACACGGACAATCCA
CGCACGCGTCCGATGGGGTTTGGACTGAACCTACAGGGCCGACGGAAGGACGGCACGGAATTTCCGATTGAGGTGAGCCT
AAGCAGCGTGCAGACCAGCGAGGGAACACTCTCAGTCGCGTTCGTCAGCGACATCACGAAGCGGAAGCAGGCGGAGGCGG
CGTTGCAGAGGAGTGAGAGCGAGTTGCGCGCGCTGGCGAGAAGCCTGATCACAGCGCAGGAGGACGAGCGGCGGCGAGTC
GCGCGGGACCTTCATGACGACGTGACGCAGCGGCTGGCGCTGCTCTCGATCGAGATCGGCAAGTTAGCGACCGAGACCTC
AGGGTCAGTGGAGGAAATCAGGAAGCGGCTTCGCTATTTTCAGAGCCAGGCCCGGCTTGCATCGAACGAGGTCCGGCGGC
TATCCCACGGTCTGCATCCTTCGGTGATCGAGGATTTTGGGCTGAGCACGGCACTGGAGGACTTTTGCCAAGAGTTCGCC
AGGGCCCAGGGGATCACCCTCATATTCGACGCCCTGGTCGCGGATGCGGTACTCAGCGCGGGCGATGCATCCTGTCTCTA
CCGGGTGGCACAGGAGTGTTTGCGGAACGTGGCCAAGCACTCCGGCGCCACCGAGGTGCGGGTGGGGATCGCGCGGGACG
GTGCAAACGTGCGGCTCACGATTAAGGACAACGGTTCAGGTTTTCATGCCGGTGCGGATCGCGTGAACAAGGGCCTCGGA
TTGGTCAGCATGAAGGAGCGGGTCAAAATGGCGAACGGAACTCTTTTGATCACCTCCCAGCCGGGACAAGGTACGGAGAT
TGTCGCCAGCGTTCCACTAACGGGAGGAGCGGCTTGA

Upstream 100 bases:

>100_bases
CGGGTCGAATGTCAACCCGAAATGACATTGCGTATATCTGCTAAGTTACTTTTGATACTGGAGTTTGTGATCCGTTGAGC
GTTTTAGCGAGGGTGCTGGG

Downstream 100 bases:

>100_bases
GAAAATTGCGCATTCTGCTGGGAGATGACCATGCCATGATCCTGAGCGGGATCAAGGGACTGCTTGAAGGTCATTACGAG
GTGATCGGGTCCGAGAAGAA

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 838; Mature: 837

Protein sequence:

>838_residues
MSDEGAVKPDFERLFNSVTGDYLVVDANLRVVAASDGYLQEAMVSRTDILGLDVFEAFRGKRHSEHTVRMEPEYPQPLEP
DELSGRLSVANDELVKRAEELQAALDAAPVAMWIAHDPKCLRITGNAYADKILMGAEWGGDRSASAQSRDVAVSYRAFRN
GMELLPEDLPAQVAAAAGKPVPEGEIELVFSDRRTVNMLMSAVPLYNAEGQVRGSVAVGVDVTARKRAEAALRDSEEKFR
TLANAIPQLCSMANADGWISWYNQRWYEYTGTTPEQMEGWGWQSVHDPDTLAEVLDRWKHSISSGEPFDMTFPLRGADGV
FRPFLTRIMAVRDRDGMVTGWFGTNTDVSEQQKIEEALRGSEELLKTFVRHVPAAVAMLDREMRYLQVSDRWYADYSLIN
GEILGRSHYEVFPDIPEHWKQLHSRCLAGETLRADEDCWEHADGETTWLRWEIRPWGDQTEKSAGKPAGVLIFTEDITER
KRIEASLREREATIRALLETAAQSILAVDAKGVVVLANRMAEEMFGFGPSELPGMRLEQLLPERSREGHVFYRAEYTDNP
RTRPMGFGLNLQGRRKDGTEFPIEVSLSSVQTSEGTLSVAFVSDITKRKQAEAALQRSESELRALARSLITAQEDERRRV
ARDLHDDVTQRLALLSIEIGKLATETSGSVEEIRKRLRYFQSQARLASNEVRRLSHGLHPSVIEDFGLSTALEDFCQEFA
RAQGITLIFDALVADAVLSAGDASCLYRVAQECLRNVAKHSGATEVRVGIARDGANVRLTIKDNGSGFHAGADRVNKGLG
LVSMKERVKMANGTLLITSQPGQGTEIVASVPLTGGAA

Sequences:

>Translated_838_residues
MSDEGAVKPDFERLFNSVTGDYLVVDANLRVVAASDGYLQEAMVSRTDILGLDVFEAFRGKRHSEHTVRMEPEYPQPLEP
DELSGRLSVANDELVKRAEELQAALDAAPVAMWIAHDPKCLRITGNAYADKILMGAEWGGDRSASAQSRDVAVSYRAFRN
GMELLPEDLPAQVAAAAGKPVPEGEIELVFSDRRTVNMLMSAVPLYNAEGQVRGSVAVGVDVTARKRAEAALRDSEEKFR
TLANAIPQLCSMANADGWISWYNQRWYEYTGTTPEQMEGWGWQSVHDPDTLAEVLDRWKHSISSGEPFDMTFPLRGADGV
FRPFLTRIMAVRDRDGMVTGWFGTNTDVSEQQKIEEALRGSEELLKTFVRHVPAAVAMLDREMRYLQVSDRWYADYSLIN
GEILGRSHYEVFPDIPEHWKQLHSRCLAGETLRADEDCWEHADGETTWLRWEIRPWGDQTEKSAGKPAGVLIFTEDITER
KRIEASLREREATIRALLETAAQSILAVDAKGVVVLANRMAEEMFGFGPSELPGMRLEQLLPERSREGHVFYRAEYTDNP
RTRPMGFGLNLQGRRKDGTEFPIEVSLSSVQTSEGTLSVAFVSDITKRKQAEAALQRSESELRALARSLITAQEDERRRV
ARDLHDDVTQRLALLSIEIGKLATETSGSVEEIRKRLRYFQSQARLASNEVRRLSHGLHPSVIEDFGLSTALEDFCQEFA
RAQGITLIFDALVADAVLSAGDASCLYRVAQECLRNVAKHSGATEVRVGIARDGANVRLTIKDNGSGFHAGADRVNKGLG
LVSMKERVKMANGTLLITSQPGQGTEIVASVPLTGGAA
>Mature_837_residues
SDEGAVKPDFERLFNSVTGDYLVVDANLRVVAASDGYLQEAMVSRTDILGLDVFEAFRGKRHSEHTVRMEPEYPQPLEPD
ELSGRLSVANDELVKRAEELQAALDAAPVAMWIAHDPKCLRITGNAYADKILMGAEWGGDRSASAQSRDVAVSYRAFRNG
MELLPEDLPAQVAAAAGKPVPEGEIELVFSDRRTVNMLMSAVPLYNAEGQVRGSVAVGVDVTARKRAEAALRDSEEKFRT
LANAIPQLCSMANADGWISWYNQRWYEYTGTTPEQMEGWGWQSVHDPDTLAEVLDRWKHSISSGEPFDMTFPLRGADGVF
RPFLTRIMAVRDRDGMVTGWFGTNTDVSEQQKIEEALRGSEELLKTFVRHVPAAVAMLDREMRYLQVSDRWYADYSLING
EILGRSHYEVFPDIPEHWKQLHSRCLAGETLRADEDCWEHADGETTWLRWEIRPWGDQTEKSAGKPAGVLIFTEDITERK
RIEASLREREATIRALLETAAQSILAVDAKGVVVLANRMAEEMFGFGPSELPGMRLEQLLPERSREGHVFYRAEYTDNPR
TRPMGFGLNLQGRRKDGTEFPIEVSLSSVQTSEGTLSVAFVSDITKRKQAEAALQRSESELRALARSLITAQEDERRRVA
RDLHDDVTQRLALLSIEIGKLATETSGSVEEIRKRLRYFQSQARLASNEVRRLSHGLHPSVIEDFGLSTALEDFCQEFAR
AQGITLIFDALVADAVLSAGDASCLYRVAQECLRNVAKHSGATEVRVGIARDGANVRLTIKDNGSGFHAGADRVNKGLGL
VSMKERVKMANGTLLITSQPGQGTEIVASVPLTGGAA

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=196, Percent_Identity=29.0816326530612, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87082311, Length=242, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 93018; Mature: 92887

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEGAVKPDFERLFNSVTGDYLVVDANLRVVAASDGYLQEAMVSRTDILGLDVFEAFRG
CCCCCCCCCHHHHHHHHCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
KRHSEHTVRMEPEYPQPLEPDELSGRLSVANDELVKRAEELQAALDAAPVAMWIAHDPKC
CCCCCCEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
LRITGNAYADKILMGAEWGGDRSASAQSRDVAVSYRAFRNGMELLPEDLPAQVAAAAGKP
EEEECCHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHCCCC
VPEGEIELVFSDRRTVNMLMSAVPLYNAEGQVRGSVAVGVDVTARKRAEAALRDSEEKFR
CCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHH
TLANAIPQLCSMANADGWISWYNQRWYEYTGTTPEQMEGWGWQSVHDPDTLAEVLDRWKH
HHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHH
SISSGEPFDMTFPLRGADGVFRPFLTRIMAVRDRDGMVTGWFGTNTDVSEQQKIEEALRG
HCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCC
SEELLKTFVRHVPAAVAMLDREMRYLQVSDRWYADYSLINGEILGRSHYEVFPDIPEHWK
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCEEECCEEECCCCCCCCCCCHHHHH
QLHSRCLAGETLRADEDCWEHADGETTWLRWEIRPWGDQTEKSAGKPAGVLIFTEDITER
HHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCHHHCCCCCCEEEEEECCHHHH
KRIEASLREREATIRALLETAAQSILAVDAKGVVVLANRMAEEMFGFGPSELPGMRLEQL
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHH
LPERSREGHVFYRAEYTDNPRTRPMGFGLNLQGRRKDGTEFPIEVSLSSVQTSEGTLSVA
CCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCCCCEEEEECCEECCCCCEEEH
FVSDITKRKQAEAALQRSESELRALARSLITAQEDERRRVARDLHDDVTQRLALLSIEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLATETSGSVEEIRKRLRYFQSQARLASNEVRRLSHGLHPSVIEDFGLSTALEDFCQEFA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHH
RAQGITLIFDALVADAVLSAGDASCLYRVAQECLRNVAKHSGATEVRVGIARDGANVRLT
HHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEE
IKDNGSGFHAGADRVNKGLGLVSMKERVKMANGTLLITSQPGQGTEIVASVPLTGGAA
EEECCCCCCCCHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SDEGAVKPDFERLFNSVTGDYLVVDANLRVVAASDGYLQEAMVSRTDILGLDVFEAFRG
CCCCCCCCHHHHHHHHCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
KRHSEHTVRMEPEYPQPLEPDELSGRLSVANDELVKRAEELQAALDAAPVAMWIAHDPKC
CCCCCCEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
LRITGNAYADKILMGAEWGGDRSASAQSRDVAVSYRAFRNGMELLPEDLPAQVAAAAGKP
EEEECCHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHCCCC
VPEGEIELVFSDRRTVNMLMSAVPLYNAEGQVRGSVAVGVDVTARKRAEAALRDSEEKFR
CCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHH
TLANAIPQLCSMANADGWISWYNQRWYEYTGTTPEQMEGWGWQSVHDPDTLAEVLDRWKH
HHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHH
SISSGEPFDMTFPLRGADGVFRPFLTRIMAVRDRDGMVTGWFGTNTDVSEQQKIEEALRG
HCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCC
SEELLKTFVRHVPAAVAMLDREMRYLQVSDRWYADYSLINGEILGRSHYEVFPDIPEHWK
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCEEECCEEECCCCCCCCCCCHHHHH
QLHSRCLAGETLRADEDCWEHADGETTWLRWEIRPWGDQTEKSAGKPAGVLIFTEDITER
HHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCHHHCCCCCCEEEEEECCHHHH
KRIEASLREREATIRALLETAAQSILAVDAKGVVVLANRMAEEMFGFGPSELPGMRLEQL
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHH
LPERSREGHVFYRAEYTDNPRTRPMGFGLNLQGRRKDGTEFPIEVSLSSVQTSEGTLSVA
CCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCCCCEEEEECCEECCCCCEEEH
FVSDITKRKQAEAALQRSESELRALARSLITAQEDERRRVARDLHDDVTQRLALLSIEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLATETSGSVEEIRKRLRYFQSQARLASNEVRRLSHGLHPSVIEDFGLSTALEDFCQEFA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHH
RAQGITLIFDALVADAVLSAGDASCLYRVAQECLRNVAKHSGATEVRVGIARDGANVRLT
HHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEE
IKDNGSGFHAGADRVNKGLGLVSMKERVKMANGTLLITSQPGQGTEIVASVPLTGGAA
EEECCCCCCCCHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]