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The map label for this gene is fmt

Identifier: 116621864

GI number: 116621864

Start: 3484477

End: 3485412

Strand: Direct

Name: fmt

Synonym: Acid_2747

Alternate gene names: 116621864

Gene position: 3484477-3485412 (Clockwise)

Preceding gene: 116621863

Following gene: 116621865

Centisome position: 34.96

GC content: 66.24

Gene sequence:

>936_bases
ATGCGGCTGGTCTTCCTCGGCACGCCCGCCTTCGCCGTGCCGACGCTCGAAGCCGTGGTCCGCGCCGGCCATGAAGTGGC
GGCCGTCCTGACCCAGCCCGACCGGCCGCGTGGCCGCGGCCAGAATCCCGCCGCCTCGCCCGTCAAGCAGGCGGCGTTGG
CCCTCAGCCTCGAGGTCTACCAGCCGGAGCGCGTCCGGCGTCCCGAACCCGTGGAATTCCTGCGCGGCATCGGTGCGCGC
GCCATGGTGATCGTCGGATATGGCCAAATCATTCCCCAAAACGTGATCGATCTCGCGCCCCTCGGCATCATCAACGTACA
CGCCTCGCTGCTTCCGAAGTATCGTGGCGCCGGACCGATCCAGTGGTCCATCGTCAACGGCGAAACCCGCACCGGCGTCA
CCACTATGCGGATCGATGCCGGGCTCGATACCGGCGATATGCTGCTTAAGCGCGATACCGAAATCGGCCCTGAAGAAAAC
GCCATGGAACTCGGCGCCCGGCTCGCCGTGCTGGGCGCGGACCTTTTGGTGAAAACGCTGGCCGGCCTCGAGGCCGGCAC
CATCGTTCCCGAAAAACAGGACGATTCCCAAGCTACGTTGGCTCCACTGCTCAAGAAGGAGGATGGCCGTATCGACTGGG
CACAGCCCGCGCTCGCCATCCACAATCGCGTCCGCGGCCTACAACCCTGGCCCGGCGCCCAGACCACCTTCCGCGGCCAG
CCGCTGCACATCTGGAAGTCACGCCCCGTCACTGCCGCGCAAGCTGCCGCGCCGGGCGCCGTACTTCGCACCCGCCCGCT
GCTTGTAGCCAGCGGCGAAGGAGCGCTCGAACTGCTCGAAGTTCAACAGGAAGGCCGCAAGCGCATTCCCGCCGCCGATT
TCGCCAACGGGCAGCGCTTGACGGAGAATGAGAAGTTAGGAGAGGGACATCTTTGA

Upstream 100 bases:

>100_bases
CGGCCGCCTCTACATCACTCACATTAGCGCCCTCAAACGCGACCTGATGAAGCGCAAGATCAAAAAGCTGCAGCGCGCCG
GGGACTGGAGTTAGACCCCC

Downstream 100 bases:

>100_bases
ATCTTCCGCTGGGATACGCTTACGCCACCACCTACGCCGGAATCCGTGCGGTGGAGAAGGATGATCTCGCTTTGATCGTC
ACCGGCCTGCCGGCTAACGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MRLVFLGTPAFAVPTLEAVVRAGHEVAAVLTQPDRPRGRGQNPAASPVKQAALALSLEVYQPERVRRPEPVEFLRGIGAR
AMVIVGYGQIIPQNVIDLAPLGIINVHASLLPKYRGAGPIQWSIVNGETRTGVTTMRIDAGLDTGDMLLKRDTEIGPEEN
AMELGARLAVLGADLLVKTLAGLEAGTIVPEKQDDSQATLAPLLKKEDGRIDWAQPALAIHNRVRGLQPWPGAQTTFRGQ
PLHIWKSRPVTAAQAAAPGAVLRTRPLLVASGEGALELLEVQQEGRKRIPAADFANGQRLTENEKLGEGHL

Sequences:

>Translated_311_residues
MRLVFLGTPAFAVPTLEAVVRAGHEVAAVLTQPDRPRGRGQNPAASPVKQAALALSLEVYQPERVRRPEPVEFLRGIGAR
AMVIVGYGQIIPQNVIDLAPLGIINVHASLLPKYRGAGPIQWSIVNGETRTGVTTMRIDAGLDTGDMLLKRDTEIGPEEN
AMELGARLAVLGADLLVKTLAGLEAGTIVPEKQDDSQATLAPLLKKEDGRIDWAQPALAIHNRVRGLQPWPGAQTTFRGQ
PLHIWKSRPVTAAQAAAPGAVLRTRPLLVASGEGALELLEVQQEGRKRIPAADFANGQRLTENEKLGEGHL
>Mature_311_residues
MRLVFLGTPAFAVPTLEAVVRAGHEVAAVLTQPDRPRGRGQNPAASPVKQAALALSLEVYQPERVRRPEPVEFLRGIGAR
AMVIVGYGQIIPQNVIDLAPLGIINVHASLLPKYRGAGPIQWSIVNGETRTGVTTMRIDAGLDTGDMLLKRDTEIGPEEN
AMELGARLAVLGADLLVKTLAGLEAGTIVPEKQDDSQATLAPLLKKEDGRIDWAQPALAIHNRVRGLQPWPGAQTTFRGQ
PLHIWKSRPVTAAQAAAPGAVLRTRPLLVASGEGALELLEVQQEGRKRIPAADFANGQRLTENEKLGEGHL

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=314, Percent_Identity=29.9363057324841, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI238814322, Length=310, Percent_Identity=29.0322580645161, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI164663775, Length=318, Percent_Identity=28.6163522012579, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789683, Length=301, Percent_Identity=43.5215946843854, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1788589, Length=283, Percent_Identity=34.2756183745583, Blast_Score=171, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI133930964, Length=340, Percent_Identity=27.0588235294118, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319458, Length=249, Percent_Identity=31.7269076305221, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550868, Length=303, Percent_Identity=32.6732673267327, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI28571984, Length=222, Percent_Identity=37.3873873873874, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24585660, Length=332, Percent_Identity=28.6144578313253, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_SOLUE (Q023V5)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_824020.1
- ProteinModelPortal:   Q023V5
- SMR:   Q023V5
- STRING:   Q023V5
- GeneID:   4429105
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_2747
- NMPDR:   fig|234267.9.peg.2623
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   VVAYGAI
- PhylomeDB:   Q023V5
- ProtClustDB:   CLSK775137
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33348; Mature: 33348

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVFLGTPAFAVPTLEAVVRAGHEVAAVLTQPDRPRGRGQNPAASPVKQAALALSLEVY
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHEEEEEE
QPERVRRPEPVEFLRGIGARAMVIVGYGQIIPQNVIDLAPLGIINVHASLLPKYRGAGPI
CCHHCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCEEHHHHHHHHHCCCCCE
QWSIVNGETRTGVTTMRIDAGLDTGDMLLKRDTEIGPEENAMELGARLAVLGADLLVKTL
EEEEECCCCCCCEEEEEEECCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGLEAGTIVPEKQDDSQATLAPLLKKEDGRIDWAQPALAIHNRVRGLQPWPGAQTTFRGQ
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PLHIWKSRPVTAAQAAAPGAVLRTRPLLVASGEGALELLEVQQEGRKRIPAADFANGQRL
CEEEECCCCCCHHHHCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TENEKLGEGHL
CCCCCCCCCCC
>Mature Secondary Structure
MRLVFLGTPAFAVPTLEAVVRAGHEVAAVLTQPDRPRGRGQNPAASPVKQAALALSLEVY
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHEEEEEE
QPERVRRPEPVEFLRGIGARAMVIVGYGQIIPQNVIDLAPLGIINVHASLLPKYRGAGPI
CCHHCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCCCCEEHHHHHHHHHCCCCCE
QWSIVNGETRTGVTTMRIDAGLDTGDMLLKRDTEIGPEENAMELGARLAVLGADLLVKTL
EEEEECCCCCCCEEEEEEECCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGLEAGTIVPEKQDDSQATLAPLLKKEDGRIDWAQPALAIHNRVRGLQPWPGAQTTFRGQ
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PLHIWKSRPVTAAQAAAPGAVLRTRPLLVASGEGALELLEVQQEGRKRIPAADFANGQRL
CEEEECCCCCCHHHHCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TENEKLGEGHL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA