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The map label for this gene is def [H]

Identifier: 116621863

GI number: 116621863

Start: 3483955

End: 3484470

Strand: Direct

Name: def [H]

Synonym: Acid_2746

Alternate gene names: 116621863

Gene position: 3483955-3484470 (Clockwise)

Preceding gene: 116621862

Following gene: 116621864

Centisome position: 34.96

GC content: 58.72

Gene sequence:

>516_bases
ATGGTTTATCCGATTGTGAAGTTTGGCGACCCCGTGCTCGAACGCGAGGCCGATACCGTAACTGAGTTCGATACTCCGGA
ACTGCACAAGTTCCTGGACGATATGTTCGAATCGATGTACGCCGCCAAGGGCGTGGGCCTTGCCGCCCCCCAGATCGGCG
TATCGAAGAAGATTGCCGTCATCGATGTTTCGAACGGCGAGAACGCGGACGACAAGCTCGTCATCATCAATCCTAAGATT
CTTAAGATCGATGGCAAGCAGGAAGGCGAAGAGGGCTGCCTCAGTATTCCCGGCTTCCGCGAGCAGGTGCGCCGCGCCCG
CTGCGTCACCATCCGCGCGCAGAATGCCAAGGGCGAGGAGTTCGAGAAGACCGGCGAAGACCTGCTCGCGCGCGCCTTCC
TCCATGAGACCGACCACCTTTACGGCCGCCTCTACATCACTCACATTAGCGCCCTCAAACGCGACCTGATGAAGCGCAAG
ATCAAAAAGCTGCAGCGCGCCGGGGACTGGAGTTAG

Upstream 100 bases:

>100_bases
GCACAGGCCTTTTTGGAAAAGTTCGGCGGCGATTCCCTGACCGAAACGCGCCGCAATTTCGATAGCTACCTGGAACAGGT
GAAGAACTACTAATAGCGAT

Downstream 100 bases:

>100_bases
ACCCCCATGCGGCTGGTCTTCCTCGGCACGCCCGCCTTCGCCGTGCCGACGCTCGAAGCCGTGGTCCGCGCCGGCCATGA
AGTGGCGGCCGTCCTGACCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MVYPIVKFGDPVLEREADTVTEFDTPELHKFLDDMFESMYAAKGVGLAAPQIGVSKKIAVIDVSNGENADDKLVIINPKI
LKIDGKQEGEEGCLSIPGFREQVRRARCVTIRAQNAKGEEFEKTGEDLLARAFLHETDHLYGRLYITHISALKRDLMKRK
IKKLQRAGDWS

Sequences:

>Translated_171_residues
MVYPIVKFGDPVLEREADTVTEFDTPELHKFLDDMFESMYAAKGVGLAAPQIGVSKKIAVIDVSNGENADDKLVIINPKI
LKIDGKQEGEEGCLSIPGFREQVRRARCVTIRAQNAKGEEFEKTGEDLLARAFLHETDHLYGRLYITHISALKRDLMKRK
IKKLQRAGDWS
>Mature_171_residues
MVYPIVKFGDPVLEREADTVTEFDTPELHKFLDDMFESMYAAKGVGLAAPQIGVSKKIAVIDVSNGENADDKLVIINPKI
LKIDGKQEGEEGCLSIPGFREQVRRARCVTIRAQNAKGEEFEKTGEDLLARAFLHETDHLYGRLYITHISALKRDLMKRK
IKKLQRAGDWS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=44.2424242424242, Blast_Score=133, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=31.2101910828025, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24645726, Length=162, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19274; Mature: 19274

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYPIVKFGDPVLEREADTVTEFDTPELHKFLDDMFESMYAAKGVGLAAPQIGVSKKIAV
CCCCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
IDVSNGENADDKLVIINPKILKIDGKQEGEEGCLSIPGFREQVRRARCVTIRAQNAKGEE
EECCCCCCCCCEEEEECCEEEEECCCCCCCCCCEECCCHHHHHHHHHEEEEEECCCCCHH
FEKTGEDLLARAFLHETDHLYGRLYITHISALKRDLMKRKIKKLQRAGDWS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVYPIVKFGDPVLEREADTVTEFDTPELHKFLDDMFESMYAAKGVGLAAPQIGVSKKIAV
CCCCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
IDVSNGENADDKLVIINPKILKIDGKQEGEEGCLSIPGFREQVRRARCVTIRAQNAKGEE
EECCCCCCCCCEEEEECCEEEEECCCCCCCCCCEECCCHHHHHHHHHEEEEEECCCCCHH
FEKTGEDLLARAFLHETDHLYGRLYITHISALKRDLMKRKIKKLQRAGDWS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA