Click here to switch to the map view.

The map label for this gene is yhcR [H]

Identifier: 116621750

GI number: 116621750

Start: 3329586

End: 3330905

Strand: Reverse

Name: yhcR [H]

Synonym: Acid_2632

Alternate gene names: 116621750

Gene position: 3330905-3329586 (Counterclockwise)

Preceding gene: 116621753

Following gene: 116621749

Centisome position: 33.42

GC content: 62.8

Gene sequence:

>1320_bases
ATGATCAGGTCCACCTCGCGCCGGCTGGCGCTGTTATTGATCGCCTGCGCGCCGCTGGCTGGAGAGGTCCGGTCGCTGAC
CATCCTGCACATCAACGATCTCCACGCGCGCCTGATGCCGCTGGAGAACAAGCATGGCGGCTTCGCCTACCTGGCCTCCG
TAATTCGCCGCGAGCGCGAGGGCTGCAACGATTGCATTCTGCTCAATGCCGGCGACGTCGCCCAAGGGACGCCGGTCTCC
ACCATTTTCCACGGGCTTCCCGTCTTCGAAGTCGCCAACCTCCTGGGCATCGACGTGGGCACGCTGGGCAATCACGATTT
CGACTATGGCTGGATGCAGGCGCGCAAGTTCATGGACACCGCGACCTACCCGATCGTCTCCGACAACATCGTCGGGCCGA
AGGGTGAATTGTTCGCCGCCAAGCCGTACGTCATTCTCAAGATCAACGGGCTGCGCGTCGCGGTCATCGGCGCGATGACG
GACGATCTGAAGACGCTCACCATTCCGGCCGCGATCGCACCCTGGCACACGCTGCCGGTGCTCGAAATCTCGATGAAATA
TGCCGCGGAGCTGAAGAGCCAGAGCGACCTGATCGTGCTGCTGGGCCACATCACGCCGCAGGAGGAATCGAAATTTCTGC
AGACCGCCACGAATATCCCGGTGTTCGTGACCGGCCACGCCCACAACGGCATGGCGCAGCCTGCGGTGCAGGATGGCCGC
ATCCTGGTCCGCGTGAAGGGCTATGCCGAGGAGCTCGGCCGCCTGGAACTGAAGGTGGATACAGAGACAAAATCCGTGGT
CTCTTCCAACTGGAAACACATCGTGGTCGATTCCACCGAGATCAAACCTGCCGCCGACGTGGCGGCGCTGGTCAAGCGCT
GGGAGGACGAGGTCAGCGCGCGCGTGGATCAGCCGCTCGCGGTGACCGCGCACGCCTTCGATAAGCGCGGCGTGAAGGCC
GTGATCGAGCAGGCGATGCGCGAGCAAAGCGGCGCCGATTTCGCCTTCATGAACATGGGCGGTGTCCGTGACACGCTGCC
CAAGGGGCAGATCCTGGTGCGCAAGATCTGGGACATCATGCCTTTCGACAATCGCGTGGTATTCGGCAAATTCAAGGGCC
GCGATTTGCCCGCCGTCGTGCTCGGCGACCGCAAGGTGGAACCGGACCGGGAATACACGCTCGCCGTCACCGATTTCACC
GCCGAGAATCAGTCCAGCCCTGAAAACCTGCGCAGCAAGGGACTCGCCTTCCCCGGGGATGGCGGACTGCTCCGCGACAT
GCTGGTGGATTGGTTCCGCAAGAAGAAGGTCGTCGAGTAG

Upstream 100 bases:

>100_bases
GGGCGAGCGCCGCACAGGCCGCCAGGCTCAAAATCGCAAGCAGACCGATCAGTCTCTTCATAACGAAAGCTAAAGTATCA
CACGTTACAATGGACGCAGT

Downstream 100 bases:

>100_bases
CGTGGTGATCGTGTTTGCGGGTCCCCCCTGCGCCGGTAAGAGCACGATCGCGGCCGAAGTGGCGCGCCGACTCGCGCTGC
CCCATCTCTCGATGGACGCC

Product: metallophosphoesterase

Products: ribonucleoside; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MIRSTSRRLALLLIACAPLAGEVRSLTILHINDLHARLMPLENKHGGFAYLASVIRREREGCNDCILLNAGDVAQGTPVS
TIFHGLPVFEVANLLGIDVGTLGNHDFDYGWMQARKFMDTATYPIVSDNIVGPKGELFAAKPYVILKINGLRVAVIGAMT
DDLKTLTIPAAIAPWHTLPVLEISMKYAAELKSQSDLIVLLGHITPQEESKFLQTATNIPVFVTGHAHNGMAQPAVQDGR
ILVRVKGYAEELGRLELKVDTETKSVVSSNWKHIVVDSTEIKPAADVAALVKRWEDEVSARVDQPLAVTAHAFDKRGVKA
VIEQAMREQSGADFAFMNMGGVRDTLPKGQILVRKIWDIMPFDNRVVFGKFKGRDLPAVVLGDRKVEPDREYTLAVTDFT
AENQSSPENLRSKGLAFPGDGGLLRDMLVDWFRKKKVVE

Sequences:

>Translated_439_residues
MIRSTSRRLALLLIACAPLAGEVRSLTILHINDLHARLMPLENKHGGFAYLASVIRREREGCNDCILLNAGDVAQGTPVS
TIFHGLPVFEVANLLGIDVGTLGNHDFDYGWMQARKFMDTATYPIVSDNIVGPKGELFAAKPYVILKINGLRVAVIGAMT
DDLKTLTIPAAIAPWHTLPVLEISMKYAAELKSQSDLIVLLGHITPQEESKFLQTATNIPVFVTGHAHNGMAQPAVQDGR
ILVRVKGYAEELGRLELKVDTETKSVVSSNWKHIVVDSTEIKPAADVAALVKRWEDEVSARVDQPLAVTAHAFDKRGVKA
VIEQAMREQSGADFAFMNMGGVRDTLPKGQILVRKIWDIMPFDNRVVFGKFKGRDLPAVVLGDRKVEPDREYTLAVTDFT
AENQSSPENLRSKGLAFPGDGGLLRDMLVDWFRKKKVVE
>Mature_439_residues
MIRSTSRRLALLLIACAPLAGEVRSLTILHINDLHARLMPLENKHGGFAYLASVIRREREGCNDCILLNAGDVAQGTPVS
TIFHGLPVFEVANLLGIDVGTLGNHDFDYGWMQARKFMDTATYPIVSDNIVGPKGELFAAKPYVILKINGLRVAVIGAMT
DDLKTLTIPAAIAPWHTLPVLEISMKYAAELKSQSDLIVLLGHITPQEESKFLQTATNIPVFVTGHAHNGMAQPAVQDGR
ILVRVKGYAEELGRLELKVDTETKSVVSSNWKHIVVDSTEIKPAADVAALVKRWEDEVSARVDQPLAVTAHAFDKRGVKA
VIEQAMREQSGADFAFMNMGGVRDTLPKGQILVRKIWDIMPFDNRVVFGKFKGRDLPAVVLGDRKVEPDREYTLAVTDFT
AENQSSPENLRSKGLAFPGDGGLLRDMLVDWFRKKKVVE

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=436, Percent_Identity=25.4587155963303, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786687, Length=453, Percent_Identity=23.6203090507726, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790658, Length=348, Percent_Identity=22.9885057471264, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19922444, Length=433, Percent_Identity=24.0184757505774, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922446, Length=406, Percent_Identity=26.3546798029557, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24654424, Length=406, Percent_Identity=26.3546798029557, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28573524, Length=417, Percent_Identity=23.7410071942446, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19921980, Length=373, Percent_Identity=21.71581769437, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652512, Length=373, Percent_Identity=21.71581769437, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161076508, Length=373, Percent_Identity=21.71581769437, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.1.3.5

Molecular weight: Translated: 48337; Mature: 48337

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSTSRRLALLLIACAPLAGEVRSLTILHINDLHARLMPLENKHGGFAYLASVIRRERE
CCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHEECCCCCCCCHHHHHHHHHHHHC
GCNDCILLNAGDVAQGTPVSTIFHGLPVFEVANLLGIDVGTLGNHDFDYGWMQARKFMDT
CCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
ATYPIVSDNIVGPKGELFAAKPYVILKINGLRVAVIGAMTDDLKTLTIPAAIAPWHTLPV
CCCCEEECCCCCCCCCEEEECCEEEEEECCEEEEEEEECCCCCEEEEECCHHCCCCCCHH
LEISMKYAAELKSQSDLIVLLGHITPQEESKFLQTATNIPVFVTGHAHNGMAQPAVQDGR
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCE
ILVRVKGYAEELGRLELKVDTETKSVVSSNWKHIVVDSTEIKPAADVAALVKRWEDEVSA
EEEEECCHHHCCCEEEEEEECCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
RVDQPLAVTAHAFDKRGVKAVIEQAMREQSGADFAFMNMGGVRDTLPKGQILVRKIWDIM
HCCCCEEEEEHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHC
PFDNRVVFGKFKGRDLPAVVLGDRKVEPDREYTLAVTDFTAENQSSPENLRSKGLAFPGD
CCCCEEEEEEECCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCC
GGLLRDMLVDWFRKKKVVE
CCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRSTSRRLALLLIACAPLAGEVRSLTILHINDLHARLMPLENKHGGFAYLASVIRRERE
CCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHEECCCCCCCCHHHHHHHHHHHHC
GCNDCILLNAGDVAQGTPVSTIFHGLPVFEVANLLGIDVGTLGNHDFDYGWMQARKFMDT
CCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
ATYPIVSDNIVGPKGELFAAKPYVILKINGLRVAVIGAMTDDLKTLTIPAAIAPWHTLPV
CCCCEEECCCCCCCCCEEEECCEEEEEECCEEEEEEEECCCCCEEEEECCHHCCCCCCHH
LEISMKYAAELKSQSDLIVLLGHITPQEESKFLQTATNIPVFVTGHAHNGMAQPAVQDGR
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCE
ILVRVKGYAEELGRLELKVDTETKSVVSSNWKHIVVDSTEIKPAADVAALVKRWEDEVSA
EEEEECCHHHCCCEEEEEEECCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
RVDQPLAVTAHAFDKRGVKAVIEQAMREQSGADFAFMNMGGVRDTLPKGQILVRKIWDIM
HCCCCEEEEEHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHC
PFDNRVVFGKFKGRDLPAVVLGDRKVEPDREYTLAVTDFTAENQSSPENLRSKGLAFPGD
CCCCEEEEEEECCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCC
GGLLRDMLVDWFRKKKVVE
CCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5'-ribonucleotide; H2O

Specific reaction: A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]