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The map label for this gene is rspA [C]

Identifier: 116621746

GI number: 116621746

Start: 3324131

End: 3325369

Strand: Reverse

Name: rspA [C]

Synonym: Acid_2628

Alternate gene names: 116621746

Gene position: 3325369-3324131 (Counterclockwise)

Preceding gene: 116621749

Following gene: 116621744

Centisome position: 33.37

GC content: 62.79

Gene sequence:

>1239_bases
ATGAACCGGCGTGATTTCGTGAGAACCTCCGCGGGCGCCACCCTGCTCGCCTCCACCCTGCAATCCACCCCGCTGGACGC
ACCGGCGGACGAGAAGGACTCGGCCATCCCCGATTACCAGAAGCCCGTCTTCAACATCCACAAGTTCTTCCCCGCCCCGG
TGAAGATCGCTTCCATCGAGCTGCTGCAATCCGGCAAGCAGTTCTTCGTCCGCACCCGCTCCACCGAGGGCGTAGAGGGC
GTCGTGCTGACCAAGGACATGGAGGATTTCGTCCCCATCCTGCAGCGCCGCGTCATCCCGCACTTCACCGGCAAAGACGC
GCGCGACCTGGAAACGCTGGTGGACGACGTCTATATCGCCAACTACAAGCTCTCCGGGCAGGCCTTCTGGTGCTGCGTGG
CCTACGTGGAGCAGAGCCTCTTCGACCTGCTGGGCAAGACCGCGCGCAAGCCGGCCGGCGAGCTCATGGGCGGCATCCTG
CGCAAGGAGATCCCGGTCTACCTCTCCGGCTCCGGCCGCGACACCACCGCCGAGGAAGAGGTCGATGTCTACGTACAGGG
CGTCCAGGCCACCGGGGCCAAGGCCGTGAAGTTCAAGATCGGCGGCCGCATGAGCGCCAATCGCGACAGCTATCCCGGCC
GCACCGACAAGATCTTCGAGATCGCCGCCAAGACCCTGGCCGGGAAAGTCACGCTGATGGCCGACGCCAACGGCAGCTAC
AGCGCCGCCAAGGCCATCGAAATCGGCAAACACCTGGAGGAGATGAAGTACCTCTGGTTCGAAGAACCCTGCCCGTGGGA
GGAACTCAGCGAGACCAAGAAAGTGGCCGACGCGCTGACGATGAAGGTCGCCTTCGGCGAGCAGAATTCCAGTCTCTGGC
AATTTCAGTGGATGATCGAAAACAAGGTGATGGACGTGGTGCAGCCCGACCTCAACTACAACGGCGGCTTCACCCGCGCA
GCCCGCGTGGCACGCATGGCGCGCAAGCAGAACATGTGGATCTGCCCGCACAACACCCAGACCGGCGCCGCCTCCGTCAA
CATCCTGCAGTTCGCGGCCACCACGCCGAACATCGGCCCGTGGATGGAGTACGTCTCCCGTGGTCCGGCAAAGAAGGAAT
CCTGGTACACGCCGAACTTCGAGATCAAGAACGGCGTGATCCCGGTACCCACCGGCCATGGGATGGGACTGCAATTCGAT
CCGGATTACTTGAAGAAGGCGACGTTGATTAAGGGGTAA

Upstream 100 bases:

>100_bases
CCAGACCGCCGCCGCAACTGCCTTCATGCACACGTCTCCTTCACGATGAGTATGACGCTCTTCGTGGCGTCTTGGTTAGC
CCCGCGTTATGCTGTGTGTC

Downstream 100 bases:

>100_bases
AGTTTTTTTCTCGCAGAGACGCAGAGCCGCAGAGTAACACTGAGAAGAAAAAACAAGAAGGCCTTAAGGGTTTAATTTTC
TCTGCGTGACTCTGCGGCTC

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNRRDFVRTSAGATLLASTLQSTPLDAPADEKDSAIPDYQKPVFNIHKFFPAPVKIASIELLQSGKQFFVRTRSTEGVEG
VVLTKDMEDFVPILQRRVIPHFTGKDARDLETLVDDVYIANYKLSGQAFWCCVAYVEQSLFDLLGKTARKPAGELMGGIL
RKEIPVYLSGSGRDTTAEEEVDVYVQGVQATGAKAVKFKIGGRMSANRDSYPGRTDKIFEIAAKTLAGKVTLMADANGSY
SAAKAIEIGKHLEEMKYLWFEEPCPWEELSETKKVADALTMKVAFGEQNSSLWQFQWMIENKVMDVVQPDLNYNGGFTRA
ARVARMARKQNMWICPHNTQTGAASVNILQFAATTPNIGPWMEYVSRGPAKKESWYTPNFEIKNGVIPVPTGHGMGLQFD
PDYLKKATLIKG

Sequences:

>Translated_412_residues
MNRRDFVRTSAGATLLASTLQSTPLDAPADEKDSAIPDYQKPVFNIHKFFPAPVKIASIELLQSGKQFFVRTRSTEGVEG
VVLTKDMEDFVPILQRRVIPHFTGKDARDLETLVDDVYIANYKLSGQAFWCCVAYVEQSLFDLLGKTARKPAGELMGGIL
RKEIPVYLSGSGRDTTAEEEVDVYVQGVQATGAKAVKFKIGGRMSANRDSYPGRTDKIFEIAAKTLAGKVTLMADANGSY
SAAKAIEIGKHLEEMKYLWFEEPCPWEELSETKKVADALTMKVAFGEQNSSLWQFQWMIENKVMDVVQPDLNYNGGFTRA
ARVARMARKQNMWICPHNTQTGAASVNILQFAATTPNIGPWMEYVSRGPAKKESWYTPNFEIKNGVIPVPTGHGMGLQFD
PDYLKKATLIKG
>Mature_412_residues
MNRRDFVRTSAGATLLASTLQSTPLDAPADEKDSAIPDYQKPVFNIHKFFPAPVKIASIELLQSGKQFFVRTRSTEGVEG
VVLTKDMEDFVPILQRRVIPHFTGKDARDLETLVDDVYIANYKLSGQAFWCCVAYVEQSLFDLLGKTARKPAGELMGGIL
RKEIPVYLSGSGRDTTAEEEVDVYVQGVQATGAKAVKFKIGGRMSANRDSYPGRTDKIFEIAAKTLAGKVTLMADANGSY
SAAKAIEIGKHLEEMKYLWFEEPCPWEELSETKKVADALTMKVAFGEQNSSLWQFQWMIENKVMDVVQPDLNYNGGFTRA
ARVARMARKQNMWICPHNTQTGAASVNILQFAATTPNIGPWMEYVSRGPAKKESWYTPNFEIKNGVIPVPTGHGMGLQFD
PDYLKKATLIKG

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=23.2673267326733, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994953, Length=242, Percent_Identity=26.0330578512397, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI226510960, Length=334, Percent_Identity=24.5508982035928, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 45787; Mature: 45787

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDFVRTSAGATLLASTLQSTPLDAPADEKDSAIPDYQKPVFNIHKFFPAPVKIASIE
CCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEHHHHH
LLQSGKQFFVRTRSTEGVEGVVLTKDMEDFVPILQRRVIPHFTGKDARDLETLVDDVYIA
HHHCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEE
NYKLSGQAFWCCVAYVEQSLFDLLGKTARKPAGELMGGILRKEIPVYLSGSGRDTTAEEE
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCC
VDVYVQGVQATGAKAVKFKIGGRMSANRDSYPGRTDKIFEIAAKTLAGKVTLMADANGSY
EEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCC
SAAKAIEIGKHLEEMKYLWFEEPCPWEELSETKKVADALTMKVAFGEQNSSLWQFQWMIE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEEEHH
NKVMDVVQPDLNYNGGFTRAARVARMARKQNMWICPHNTQTGAASVNILQFAATTPNIGP
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEEEECCCCCCHH
WMEYVSRGPAKKESWYTPNFEIKNGVIPVPTGHGMGLQFDPDYLKKATLIKG
HHHHHHCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNRRDFVRTSAGATLLASTLQSTPLDAPADEKDSAIPDYQKPVFNIHKFFPAPVKIASIE
CCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEHHHHH
LLQSGKQFFVRTRSTEGVEGVVLTKDMEDFVPILQRRVIPHFTGKDARDLETLVDDVYIA
HHHCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEE
NYKLSGQAFWCCVAYVEQSLFDLLGKTARKPAGELMGGILRKEIPVYLSGSGRDTTAEEE
EEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCC
VDVYVQGVQATGAKAVKFKIGGRMSANRDSYPGRTDKIFEIAAKTLAGKVTLMADANGSY
EEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCC
SAAKAIEIGKHLEEMKYLWFEEPCPWEELSETKKVADALTMKVAFGEQNSSLWQFQWMIE
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEEEHH
NKVMDVVQPDLNYNGGFTRAARVARMARKQNMWICPHNTQTGAASVNILQFAATTPNIGP
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEEEECCCCCCHH
WMEYVSRGPAKKESWYTPNFEIKNGVIPVPTGHGMGLQFDPDYLKKATLIKG
HHHHHHCCCCCCCCCCCCCEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8277241 [H]