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The map label for this gene is emrA [H]

Identifier: 116621741

GI number: 116621741

Start: 3315635

End: 3316981

Strand: Reverse

Name: emrA [H]

Synonym: Acid_2623

Alternate gene names: 116621741

Gene position: 3316981-3315635 (Counterclockwise)

Preceding gene: 116621742

Following gene: 116621740

Centisome position: 33.28

GC content: 64.66

Gene sequence:

>1347_bases
ATGGTAGACGACCGAACCCCTTATGCATCGCACCAGGAAATCGAACAACTGCGCGACGAGCTTCGCCGTCTGAAAGAAGA
GCAGGAAAAGCAAAAGAACGGCCGGCACGAAGACGGACAGCCGAAGGACGAGAAGAAAGAAGAGAGCGGCGAGGAAAACG
AGAAGCCGGAGGAGCCGAAAAAGCCGCACCCCGTGCGCAAGTTCATCCTCATCGCCCTGGTGCTCGTGTTGGCCATCGGC
GGCCTCCTCTGGTGGCTCCATGCGCGGAATTTCGAAAGCACCGACGACGCGATCGTGGATGGCCACACCAGCGGACTCGC
CGCCCGCGTCGCGGGCACCGTGATCGCCGTGTACGTGGAAGAAAATCAATTTGTCAAAGCCGGCCAGGTGGTGGTCGATC
TCGACCCTCGCGATTACGAAGCCGCCGTCGCCTCCGCCCGCGGGCAGCTCCAGCAGGCCCAAGCACAACAGCAGGCCGAG
CTGCCCAGTGTCCCCGTGACGCGCGTCACAAGCCAGACCAACATCGCCAATGCCGGCTCCTCTATTGCGAGCGCCGAAGC
CGGTGTCGCTGCCGCGCAGCGCAATTACGAGGCTGCGCTCGATCGCGTCCGCGAGGCACAGGCCACCGCCGCCAAAGCAC
AGTCGGACGTTGATCGCTATCGTCCGCTTGCCGAGAAAGACGAAGTCCCGCGCGAGCAATTTGACCAGGTGGTTGCCACC
GCCACCTCCAGCGCCGCCGCGGTCGCCGCCAATCAGGCCGGCGCGCAAGCCGCGCTGAAACAAGTAGATCAGGCTCGCGC
GCAGCTTGCGCAAGCACGGCAAACCGCCGGCGAAGTCGCCAACAATGCCCCGCGCCAGATCGCGATCCAACAGGCCAACG
TGCGCAGCCGCGCAGGCAGCGCGCAGATGTCGCGCGCCCAGCTCGAGCAGGCCCTGCTCAATCTCTCCTACTGCAAGATC
CAGACTCCCGTGAGCGGAATCGTCGCCCGTCGCGTCGCCGAAATCGGCCAGCACGTCGCCCCCGGCCAGGAGGTGCTTCT
GGTCACGCGGCTCGACGATCTCTGGGTGACCGCCAACTTTCGCGAAACCCAGATCCGCCGCATGGTAGCCGGCCAGTCCG
CCCGCATTCACGTTGACGCGCTCGCTCAGGATTTCGATGGCTACGTGGAAAGCATGCCCGCCGCCAGCGGCTCTATCACC
AGCCTGCTGCCGCCCGAAAATGCCACGGGCAATTTCATCAAGGTGGTGCAGCGGCTGCCAGTGCGCATCCGCTTCAAGCA
GGGCCAAAACGGGCTCGACCGCCTCCGTCCCGGCATGTCCGTCGAACCCAAAGTGAGGGTCCGGTAG

Upstream 100 bases:

>100_bases
CAGCGCGCTCTTCGCCTTCAACCTCGCGCGCGTGGCCCTCGCCCGCGCCACCGCGCAAACCGAGCAAGGCGTTACCCGCC
TGCTGCGAGAAAGGTGAGCC

Downstream 100 bases:

>100_bases
CCTCATGCCGATTGACGAATCCCAGGGCTGGCATCCCGGCCACAGTCCCTGGACGGTTGCCTTGACCGTCACGATCGCCA
CGTTCATGGAGGTGATGGAT

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MVDDRTPYASHQEIEQLRDELRRLKEEQEKQKNGRHEDGQPKDEKKEESGEENEKPEEPKKPHPVRKFILIALVLVLAIG
GLLWWLHARNFESTDDAIVDGHTSGLAARVAGTVIAVYVEENQFVKAGQVVVDLDPRDYEAAVASARGQLQQAQAQQQAE
LPSVPVTRVTSQTNIANAGSSIASAEAGVAAAQRNYEAALDRVREAQATAAKAQSDVDRYRPLAEKDEVPREQFDQVVAT
ATSSAAAVAANQAGAQAALKQVDQARAQLAQARQTAGEVANNAPRQIAIQQANVRSRAGSAQMSRAQLEQALLNLSYCKI
QTPVSGIVARRVAEIGQHVAPGQEVLLVTRLDDLWVTANFRETQIRRMVAGQSARIHVDALAQDFDGYVESMPAASGSIT
SLLPPENATGNFIKVVQRLPVRIRFKQGQNGLDRLRPGMSVEPKVRVR

Sequences:

>Translated_448_residues
MVDDRTPYASHQEIEQLRDELRRLKEEQEKQKNGRHEDGQPKDEKKEESGEENEKPEEPKKPHPVRKFILIALVLVLAIG
GLLWWLHARNFESTDDAIVDGHTSGLAARVAGTVIAVYVEENQFVKAGQVVVDLDPRDYEAAVASARGQLQQAQAQQQAE
LPSVPVTRVTSQTNIANAGSSIASAEAGVAAAQRNYEAALDRVREAQATAAKAQSDVDRYRPLAEKDEVPREQFDQVVAT
ATSSAAAVAANQAGAQAALKQVDQARAQLAQARQTAGEVANNAPRQIAIQQANVRSRAGSAQMSRAQLEQALLNLSYCKI
QTPVSGIVARRVAEIGQHVAPGQEVLLVTRLDDLWVTANFRETQIRRMVAGQSARIHVDALAQDFDGYVESMPAASGSIT
SLLPPENATGNFIKVVQRLPVRIRFKQGQNGLDRLRPGMSVEPKVRVR
>Mature_448_residues
MVDDRTPYASHQEIEQLRDELRRLKEEQEKQKNGRHEDGQPKDEKKEESGEENEKPEEPKKPHPVRKFILIALVLVLAIG
GLLWWLHARNFESTDDAIVDGHTSGLAARVAGTVIAVYVEENQFVKAGQVVVDLDPRDYEAAVASARGQLQQAQAQQQAE
LPSVPVTRVTSQTNIANAGSSIASAEAGVAAAQRNYEAALDRVREAQATAAKAQSDVDRYRPLAEKDEVPREQFDQVVAT
ATSSAAAVAANQAGAQAALKQVDQARAQLAQARQTAGEVANNAPRQIAIQQANVRSRAGSAQMSRAQLEQALLNLSYCKI
QTPVSGIVARRVAEIGQHVAPGQEVLLVTRLDDLWVTANFRETQIRRMVAGQSARIHVDALAQDFDGYVESMPAASGSIT
SLLPPENATGNFIKVVQRLPVRIRFKQGQNGLDRLRPGMSVEPKVRVR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=401, Percent_Identity=31.6708229426434, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788711, Length=394, Percent_Identity=29.1878172588832, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790519, Length=359, Percent_Identity=23.6768802228412, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48877; Mature: 48877

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDDRTPYASHQEIEQLRDELRRLKEEQEKQKNGRHEDGQPKDEKKEESGEENEKPEEPK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCC
KPHPVRKFILIALVLVLAIGGLLWWLHARNFESTDDAIVDGHTSGLAARVAGTVIAVYVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHCEEEEEEEE
ENQFVKAGQVVVDLDPRDYEAAVASARGQLQQAQAQQQAELPSVPVTRVTSQTNIANAGS
CCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCC
SIASAEAGVAAAQRNYEAALDRVREAQATAAKAQSDVDRYRPLAEKDEVPREQFDQVVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ATSSAAAVAANQAGAQAALKQVDQARAQLAQARQTAGEVANNAPRQIAIQQANVRSRAGS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHCCH
AQMSRAQLEQALLNLSYCKIQTPVSGIVARRVAEIGQHVAPGQEVLLVTRLDDLWVTANF
HHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCC
RETQIRRMVAGQSARIHVDALAQDFDGYVESMPAASGSITSLLPPENATGNFIKVVQRLP
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
VRIRFKQGQNGLDRLRPGMSVEPKVRVR
CEEEECCCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVDDRTPYASHQEIEQLRDELRRLKEEQEKQKNGRHEDGQPKDEKKEESGEENEKPEEPK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCC
KPHPVRKFILIALVLVLAIGGLLWWLHARNFESTDDAIVDGHTSGLAARVAGTVIAVYVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHCEEEEEEEE
ENQFVKAGQVVVDLDPRDYEAAVASARGQLQQAQAQQQAELPSVPVTRVTSQTNIANAGS
CCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCC
SIASAEAGVAAAQRNYEAALDRVREAQATAAKAQSDVDRYRPLAEKDEVPREQFDQVVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ATSSAAAVAANQAGAQAALKQVDQARAQLAQARQTAGEVANNAPRQIAIQQANVRSRAGS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHCCH
AQMSRAQLEQALLNLSYCKIQTPVSGIVARRVAEIGQHVAPGQEVLLVTRLDDLWVTANF
HHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCC
RETQIRRMVAGQSARIHVDALAQDFDGYVESMPAASGSITSLLPPENATGNFIKVVQRLP
HHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
VRIRFKQGQNGLDRLRPGMSVEPKVRVR
CEEEECCCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]