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The map label for this gene is nolF [H]

Identifier: 116621482

GI number: 116621482

Start: 3010418

End: 3011695

Strand: Direct

Name: nolF [H]

Synonym: Acid_2364

Alternate gene names: 116621482

Gene position: 3010418-3011695 (Clockwise)

Preceding gene: 116621481

Following gene: 116621483

Centisome position: 30.21

GC content: 65.18

Gene sequence:

>1278_bases
ATGAACCGACTTCTTCTTTCATCTCTTGCACTTGCTTCTGTATTCCTGATCTCTTGCTCGAAGAGCAAGGAGGTCACCGC
GGCCGGACCAGCCGCGCGCCAGCCCGTCGTCGTCTCGGCCGCCCCGGCGATCACCCGCACCGTCCCCGCCTCCTTCCAGG
AGACTGGAACCTTTATCGCCGATGAGACCAGCGACATCGCGCCTCTCGTCGCCGGGCGCGTCATCGCCACCCCGGTCAAT
GTCGGCGATTTCGTCAAGCAGGGGCAGGTGATCTGCGAACTCGAGCACCGCGACGCCCAGCTTCGCCTGGACCAGGCGCG
AGCCGCCCTCGATCAGGCCACCGCCGGCGTCCGCCAGTCGCAATCCCGCATCGGCCTCGCCGCCAATGGCAAGTTCGACC
CAAATAATGTCCCCGAAGCCGCCGCCGCCCGCGCCGCCTACGAATCCGCCCAGGCACAGGCCCGCCTGGCCGCCGCCGAC
GCCAAGCGCTACGAAAATCTGGTGGCATCCGGCGACGTCTCGCGCAGCGCCTTTGAGAAGGCCCACACCGCGCAGGAGAC
TGCCGAGGCCCAGGCCAATGCCGCCAAACAGCAATACGAGGCCGCCCTCAACACCGCGCGCATGAGCTACGGCGCCGTGG
AAAACTCGCAGGCATCGCTCGAGGGCGCCCGCTCCCAGCTGGCGCAGGCTGAAAAAGGCCTGTCCGATACCACCATTCGA
GCGCCCTTCGACGGCTACATCACCGCCCGCCCCGTGGCTGCCGGCGAGTATGTCGCGCTCTCCAATAAGATCGCCACTAT
CGTCCGCATCGGCACCATGAAGCTCGAATTGCAGACCCCCGAACAGCAGGCCGGACGAGCCAAACTGGGGATGGCCGTGG
TCGCCCGCGTGGCCGCCTACCCGGATCGCGATTTCAACGGACGCGTCAGCGCCATCAACCAGTCCGTGGACCCCAACTCC
CGCGTTTTCATCCTCGAAGCGAAATTTGATAATCCAAAAGGTGAACTCCGCCCCGGCATGTTTTCCACCGCCCGCGTCAT
CCTTCCCGGTGGCGAGCAGGCTACCTTCGCACCCCGCAACGCCGTCATTCGCGATAAGACCACCGACTCCTTCCAGGTCT
TCACGATCGATGGCAACACCGCCCATCTCCGCGTAGTCGTCGCCGGCGACGCGGAAGGCGATCAGATCCGCATCGTGAGC
GGTCTCAATCCCAACGACACCGTCGCCACCAGCCATCAGGCCGAGCTCTTCGATGGCGCCACGGTGCAGGTTCGGTAA

Upstream 100 bases:

>100_bases
GATTCAAGCCGAATGCCGATGATCAACAGGTAGCCAGGGACTTCACCACCATCATGATGCACGGAATTGGGACCAACTCG
ACCACTCAGGGGAAATAGTA

Downstream 100 bases:

>100_bases
CCCATGCATACACTCGCCCAACTCTGCGTTCGCCGTCCCGTTTTTGCGACCATGCTGGTTCTTTCGCTGGTCGTGGTGGG
CACCTTTTGCTATTTCAGCC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNRLLLSSLALASVFLISCSKSKEVTAAGPAARQPVVVSAAPAITRTVPASFQETGTFIADETSDIAPLVAGRVIATPVN
VGDFVKQGQVICELEHRDAQLRLDQARAALDQATAGVRQSQSRIGLAANGKFDPNNVPEAAAARAAYESAQAQARLAAAD
AKRYENLVASGDVSRSAFEKAHTAQETAEAQANAAKQQYEAALNTARMSYGAVENSQASLEGARSQLAQAEKGLSDTTIR
APFDGYITARPVAAGEYVALSNKIATIVRIGTMKLELQTPEQQAGRAKLGMAVVARVAAYPDRDFNGRVSAINQSVDPNS
RVFILEAKFDNPKGELRPGMFSTARVILPGGEQATFAPRNAVIRDKTTDSFQVFTIDGNTAHLRVVVAGDAEGDQIRIVS
GLNPNDTVATSHQAELFDGATVQVR

Sequences:

>Translated_425_residues
MNRLLLSSLALASVFLISCSKSKEVTAAGPAARQPVVVSAAPAITRTVPASFQETGTFIADETSDIAPLVAGRVIATPVN
VGDFVKQGQVICELEHRDAQLRLDQARAALDQATAGVRQSQSRIGLAANGKFDPNNVPEAAAARAAYESAQAQARLAAAD
AKRYENLVASGDVSRSAFEKAHTAQETAEAQANAAKQQYEAALNTARMSYGAVENSQASLEGARSQLAQAEKGLSDTTIR
APFDGYITARPVAAGEYVALSNKIATIVRIGTMKLELQTPEQQAGRAKLGMAVVARVAAYPDRDFNGRVSAINQSVDPNS
RVFILEAKFDNPKGELRPGMFSTARVILPGGEQATFAPRNAVIRDKTTDSFQVFTIDGNTAHLRVVVAGDAEGDQIRIVS
GLNPNDTVATSHQAELFDGATVQVR
>Mature_425_residues
MNRLLLSSLALASVFLISCSKSKEVTAAGPAARQPVVVSAAPAITRTVPASFQETGTFIADETSDIAPLVAGRVIATPVN
VGDFVKQGQVICELEHRDAQLRLDQARAALDQATAGVRQSQSRIGLAANGKFDPNNVPEAAAARAAYESAQAQARLAAAD
AKRYENLVASGDVSRSAFEKAHTAQETAEAQANAAKQQYEAALNTARMSYGAVENSQASLEGARSQLAQAEKGLSDTTIR
APFDGYITARPVAAGEYVALSNKIATIVRIGTMKLELQTPEQQAGRAKLGMAVVARVAAYPDRDFNGRVSAINQSVDPNS
RVFILEAKFDNPKGELRPGMFSTARVILPGGEQATFAPRNAVIRDKTTDSFQVFTIDGNTAHLRVVVAGDAEGDQIRIVS
GLNPNDTVATSHQAELFDGATVQVR

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790519, Length=264, Percent_Identity=25.7575757575758, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789041, Length=264, Percent_Identity=26.5151515151515, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44919; Mature: 44919

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLLLSSLALASVFLISCSKSKEVTAAGPAARQPVVVSAAPAITRTVPASFQETGTFIA
CCHHHHHHHHHHHHHHEECCCCCCEEECCCCCCCCEEEECCCHHHHCCCCCHHHCCCEEE
DETSDIAPLVAGRVIATPVNVGDFVKQGQVICELEHRDAQLRLDQARAALDQATAGVRQS
CCCCCHHHHHHCEEEEECCCHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHH
QSRIGLAANGKFDPNNVPEAAAARAAYESAQAQARLAAADAKRYENLVASGDVSRSAFEK
HHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AHTAQETAEAQANAAKQQYEAALNTARMSYGAVENSQASLEGARSQLAQAEKGLSDTTIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
APFDGYITARPVAAGEYVALSNKIATIVRIGTMKLELQTPEQQAGRAKLGMAVVARVAAY
CCCCCCEEECCCCCCCEEEECCCEEEEEEEEEEEEEEECCHHHCCHHHHHHHHHHHHHHC
PDRDFNGRVSAINQSVDPNSRVFILEAKFDNPKGELRPGMFSTARVILPGGEQATFAPRN
CCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEECCCC
AVIRDKTTDSFQVFTIDGNTAHLRVVVAGDAEGDQIRIVSGLNPNDTVATSHQAELFDGA
CEEECCCCCCEEEEEEECCCEEEEEEEECCCCCCEEEEEECCCCCCCEECCCCHHHCCCC
TVQVR
EEEEC
>Mature Secondary Structure
MNRLLLSSLALASVFLISCSKSKEVTAAGPAARQPVVVSAAPAITRTVPASFQETGTFIA
CCHHHHHHHHHHHHHHEECCCCCCEEECCCCCCCCEEEECCCHHHHCCCCCHHHCCCEEE
DETSDIAPLVAGRVIATPVNVGDFVKQGQVICELEHRDAQLRLDQARAALDQATAGVRQS
CCCCCHHHHHHCEEEEECCCHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHH
QSRIGLAANGKFDPNNVPEAAAARAAYESAQAQARLAAADAKRYENLVASGDVSRSAFEK
HHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
AHTAQETAEAQANAAKQQYEAALNTARMSYGAVENSQASLEGARSQLAQAEKGLSDTTIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
APFDGYITARPVAAGEYVALSNKIATIVRIGTMKLELQTPEQQAGRAKLGMAVVARVAAY
CCCCCCEEECCCCCCCEEEECCCEEEEEEEEEEEEEEECCHHHCCHHHHHHHHHHHHHHC
PDRDFNGRVSAINQSVDPNSRVFILEAKFDNPKGELRPGMFSTARVILPGGEQATFAPRN
CCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEECCCC
AVIRDKTTDSFQVFTIDGNTAHLRVVVAGDAEGDQIRIVSGLNPNDTVATSHQAELFDGA
CEEECCCCCCEEEEEEECCCEEEEEEEECCCCCCEEEEEECCCCCCCEECCCCHHHCCCC
TVQVR
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]