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The map label for this gene is afr [H]

Identifier: 116621360

GI number: 116621360

Start: 2865302

End: 2866366

Strand: Reverse

Name: afr [H]

Synonym: Acid_2241

Alternate gene names: 116621360

Gene position: 2866366-2865302 (Counterclockwise)

Preceding gene: 116621361

Following gene: 116621359

Centisome position: 28.76

GC content: 64.23

Gene sequence:

>1065_bases
ATGAAAATTCTGATCGTCGGACTGGGCAGCATCGGCCAGCGCCACGTGCGCAATCTGCGCGCGCTGCTGGGTGAGGAAAC
CGAACTCCTCGCATGGCGCGTCCGCCGCCTCTCCCACGTCGTCACCCCTGCCATGCAGGCCGACCAGAACCAGGAGGTCG
AGAAGGTCTACGGTATCCGCTCCTTCGATACGCTGGAGGGCGCGCTCGCCGAAGAGCCTCGGGTTGCCTTCATCTGCAAC
CCCAGCAGCATGCACGTAGCGCCGGCCCTGGCGTGCCTGCGCGCCGGCTGCGACCTGTTCCTGGAAAAGCCCGTATCCCA
CAACCTGGATGGGCTCCCGGAGTTGTTACGCGCGGCGGCGGAACGCAACCGAGTAGTCATGGTGGGCTACCAGCTTCGCT
TCCATCCCTGTTTCCTGGCAGTGCAAAAAGCGGTACGCGAGGGCAGCCTCGGCAATCTCCTGGCCGTCCGCGCGCACGTC
GGCGAATACCTGCCCGGCTGGCATCCTTATGAGGATTACCGCCAGACCTACGCCTCGCGTGCCGATCTCGGCGGCGGCGT
CATTGTCACGCAGATCCACGAGTTCGACTATCTCTACGCCCTGTTCGGCGTGCCGCGCCGCCTGTTCGCCCTCGGCGGAC
ACTGGAGCCACCTGGAAATCGACGTGGAAGACACCGCCAGCACCCTGATGGAATTCCAGGTCTCCGGCCGCCCTCTCCCG
GTCCACCTGCAGCAGGATTACCTGCAGCGCCCGCCCAGCCGCGGCTGCGAAATCATCGGCGATAGCGGCAAGATTCACAT
GGACCTCACGGGCCTCTCGGTCACCCGCTACGACCGCGAAGGCAAGGTGGCCGAAACGGCGCGCTGGGAGAATTTCGACC
GCAACCAGCTGTTTCTCGATCAACTCCGCCACTTCCTGGAGTGCGTGGAGACTCGCCGCAAACCTGTCGTGGATCTTCAG
GACGGCATCTGGAGTCTGAGGATGGCTCTCGCCGCGAGAGAATCCATCGCTTCCGGCCGAGCCGTCGAGCTAAGCGAGCA
AAGTCAGGATGCCCACCATGCCTGA

Upstream 100 bases:

>100_bases
GCGAAGCCGGCGGCGAACTCGCCTGGGCCCGCCGCAACCTCGATGTCGTGAAGCAGCAATTCGCGAAAGCCAACTGCCTC
GCCCGCGGGAACTACTCGAC

Downstream 100 bases:

>100_bases
TGTCTTCGATCTCACCGGACGCGTCGCCGCCATTACCGGCGGCGCCGGCTTACTCGGCGAACAACATGCCCGCGCCATCG
CGCGCGCCGGCGGTTTCCCG

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKILIVGLGSIGQRHVRNLRALLGEETELLAWRVRRLSHVVTPAMQADQNQEVEKVYGIRSFDTLEGALAEEPRVAFICN
PSSMHVAPALACLRAGCDLFLEKPVSHNLDGLPELLRAAAERNRVVMVGYQLRFHPCFLAVQKAVREGSLGNLLAVRAHV
GEYLPGWHPYEDYRQTYASRADLGGGVIVTQIHEFDYLYALFGVPRRLFALGGHWSHLEIDVEDTASTLMEFQVSGRPLP
VHLQQDYLQRPPSRGCEIIGDSGKIHMDLTGLSVTRYDREGKVAETARWENFDRNQLFLDQLRHFLECVETRRKPVVDLQ
DGIWSLRMALAARESIASGRAVELSEQSQDAHHA

Sequences:

>Translated_354_residues
MKILIVGLGSIGQRHVRNLRALLGEETELLAWRVRRLSHVVTPAMQADQNQEVEKVYGIRSFDTLEGALAEEPRVAFICN
PSSMHVAPALACLRAGCDLFLEKPVSHNLDGLPELLRAAAERNRVVMVGYQLRFHPCFLAVQKAVREGSLGNLLAVRAHV
GEYLPGWHPYEDYRQTYASRADLGGGVIVTQIHEFDYLYALFGVPRRLFALGGHWSHLEIDVEDTASTLMEFQVSGRPLP
VHLQQDYLQRPPSRGCEIIGDSGKIHMDLTGLSVTRYDREGKVAETARWENFDRNQLFLDQLRHFLECVETRRKPVVDLQ
DGIWSLRMALAARESIASGRAVELSEQSQDAHHA
>Mature_354_residues
MKILIVGLGSIGQRHVRNLRALLGEETELLAWRVRRLSHVVTPAMQADQNQEVEKVYGIRSFDTLEGALAEEPRVAFICN
PSSMHVAPALACLRAGCDLFLEKPVSHNLDGLPELLRAAAERNRVVMVGYQLRFHPCFLAVQKAVREGSLGNLLAVRAHV
GEYLPGWHPYEDYRQTYASRADLGGGVIVTQIHEFDYLYALFGVPRRLFALGGHWSHLEIDVEDTASTLMEFQVSGRPLP
VHLQQDYLQRPPSRGCEIIGDSGKIHMDLTGLSVTRYDREGKVAETARWENFDRNQLFLDQLRHFLECVETRRKPVVDLQ
DGIWSLRMALAARESIASGRAVELSEQSQDAHHA

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=329, Percent_Identity=24.9240121580547, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 39849; Mature: 39849

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIVGLGSIGQRHVRNLRALLGEETELLAWRVRRLSHVVTPAMQADQNQEVEKVYGIR
CEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
SFDTLEGALAEEPRVAFICNPSSMHVAPALACLRAGCDLFLEKPVSHNLDGLPELLRAAA
CHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHEECCCCCCCCCCHHHHHHHHH
ERNRVVMVGYQLRFHPCFLAVQKAVREGSLGNLLAVRAHVGEYLPGWHPYEDYRQTYASR
HCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ADLGGGVIVTQIHEFDYLYALFGVPRRLFALGGHWSHLEIDVEDTASTLMEFQVSGRPLP
CCCCCCEEEEEHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCEE
VHLQQDYLQRPPSRGCEIIGDSGKIHMDLTGLSVTRYDREGKVAETARWENFDRNQLFLD
EEECHHHHHCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCHHHHHHCCCCCCHHHHHH
QLRHFLECVETRRKPVVDLQDGIWSLRMALAARESIASGRAVELSEQSQDAHHA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKILIVGLGSIGQRHVRNLRALLGEETELLAWRVRRLSHVVTPAMQADQNQEVEKVYGIR
CEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
SFDTLEGALAEEPRVAFICNPSSMHVAPALACLRAGCDLFLEKPVSHNLDGLPELLRAAA
CHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHEECCCCCCCCCCHHHHHHHHH
ERNRVVMVGYQLRFHPCFLAVQKAVREGSLGNLLAVRAHVGEYLPGWHPYEDYRQTYASR
HCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ADLGGGVIVTQIHEFDYLYALFGVPRRLFALGGHWSHLEIDVEDTASTLMEFQVSGRPLP
CCCCCCEEEEEHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCEE
VHLQQDYLQRPPSRGCEIIGDSGKIHMDLTGLSVTRYDREGKVAETARWENFDRNQLFLD
EEECHHHHHCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCHHHHHHCCCCCCHHHHHH
QLRHFLECVETRRKPVVDLQDGIWSLRMALAARESIASGRAVELSEQSQDAHHA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA