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The map label for this gene is yfdH [C]

Identifier: 116621355

GI number: 116621355

Start: 2860335

End: 2861285

Strand: Reverse

Name: yfdH [C]

Synonym: Acid_2236

Alternate gene names: 116621355

Gene position: 2861285-2860335 (Counterclockwise)

Preceding gene: 116621356

Following gene: 116621352

Centisome position: 28.71

GC content: 57.2

Gene sequence:

>951_bases
ATGAGATTCATCAGCGTCGTCAGCGGGTGTTATAACGAGGAAGACAATATCCGCGAACTCTACGATAGGGTCTGCCGGGT
CATGGCCGACGAGCTGCCGGAGTACGCCTTCGAAATCGTCATCATCGATAACGCCTCGAAAGACGGCACGGCCAGAATTC
TCCGCGAAATCGCCGCCACCGATAAACGGCTCAAGGTCATCATTAACAACCGGAACTTCGGCCACGTTCGCTCACCCTAT
TACGGCGTGCTGCAGGCTGGCGGCGACGCCATGATTGCCATGGCCTCAGACCTCGAAGATCCGCCCGAGATGATCCCGCA
ATTCGTGCGCAAGTGGGAAGAAGGCTACAAGCTGGTCCTCGCGCAGAAGGCTTCCAGCGAGGAGTCTTCCGCCTTCTCTC
TGGTGCGCAAGTGCTACTACTTCCTGGTCAACCGCCTCTCCGACGTTCCGCTGGTTAAGGACGTGACGGGCTTCGGACTC
TATGACCGCCGCGTCATCGAAGACATGCGAAGAATCGGCGACCCCTACCCGTATATCCGCGGCATGATCTGCGACCTGGG
ATACGAGCAGTTTCTGATTCCCTTCCGCAAGCCCCTTCGCAAACGCGGCTTCAGCAAGAACAACTTCTACACGCTCTACG
ACCTCGCAATCCTCGGCATTACCAACCACTCCAAGGTCCCGCTGCGCGTCTCCGTGATGGCCGGAGTCGGCATGGGGATT
CTCAGCTTCCTGGTGGCTTTGGCCTACCTGGTATACAAGCTTCTGTTCTGGAATAATTTCAGCGTCGGCACCGCTCCCGT
GGTTATTGGAATGTTCTTTCTCGGAGCGATCCAGCTCTTCTGTATCGGTATGCTCGGCGAGTACATCGGCTCCATTCACA
CCCAGGTCCTGAAGCGCCCGCTGGTCATTGAAAAGGAGCGGATCAACTTCGACTCGGGCCCCGTCGGTTAA

Upstream 100 bases:

>100_bases
TCGAAGATGGCCACATGGTAGTCATGCACATGATTTGCTACTACTTTATGGATCATGAAAAAGTGAATCAGGAAAAACTC
GCGGGGTAGGCTGGTCCCGC

Downstream 100 bases:

>100_bases
ACATCGGCCGTACAGAACTTGCACTTCGTGGCGTCGATCGCGATCGCTTCCTTGCAGCGCGGGCAATTCTTCGTTGGCGG
TGGCGCCGGAGCCGGAGCCT

Product: glycosyl transferase family protein

Products: GDP; dolichyl D-mannosyl phosphate

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MRFISVVSGCYNEEDNIRELYDRVCRVMADELPEYAFEIVIIDNASKDGTARILREIAATDKRLKVIINNRNFGHVRSPY
YGVLQAGGDAMIAMASDLEDPPEMIPQFVRKWEEGYKLVLAQKASSEESSAFSLVRKCYYFLVNRLSDVPLVKDVTGFGL
YDRRVIEDMRRIGDPYPYIRGMICDLGYEQFLIPFRKPLRKRGFSKNNFYTLYDLAILGITNHSKVPLRVSVMAGVGMGI
LSFLVALAYLVYKLLFWNNFSVGTAPVVIGMFFLGAIQLFCIGMLGEYIGSIHTQVLKRPLVIEKERINFDSGPVG

Sequences:

>Translated_316_residues
MRFISVVSGCYNEEDNIRELYDRVCRVMADELPEYAFEIVIIDNASKDGTARILREIAATDKRLKVIINNRNFGHVRSPY
YGVLQAGGDAMIAMASDLEDPPEMIPQFVRKWEEGYKLVLAQKASSEESSAFSLVRKCYYFLVNRLSDVPLVKDVTGFGL
YDRRVIEDMRRIGDPYPYIRGMICDLGYEQFLIPFRKPLRKRGFSKNNFYTLYDLAILGITNHSKVPLRVSVMAGVGMGI
LSFLVALAYLVYKLLFWNNFSVGTAPVVIGMFFLGAIQLFCIGMLGEYIGSIHTQVLKRPLVIEKERINFDSGPVG
>Mature_316_residues
MRFISVVSGCYNEEDNIRELYDRVCRVMADELPEYAFEIVIIDNASKDGTARILREIAATDKRLKVIINNRNFGHVRSPY
YGVLQAGGDAMIAMASDLEDPPEMIPQFVRKWEEGYKLVLAQKASSEESSAFSLVRKCYYFLVNRLSDVPLVKDVTGFGL
YDRRVIEDMRRIGDPYPYIRGMICDLGYEQFLIPFRKPLRKRGFSKNNFYTLYDLAILGITNHSKVPLRVSVMAGVGMGI
LSFLVALAYLVYKLLFWNNFSVGTAPVVIGMFFLGAIQLFCIGMLGEYIGSIHTQVLKRPLVIEKERINFDSGPVG

Specific function: Involved In O Antigen Modification. Catalyzes The Transfer Of The Glucose Residue From Udp-Glucose To A Lipid Carrier (By Similarity). [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family. GtrB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788692, Length=305, Percent_Identity=32.7868852459016, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788588, Length=307, Percent_Identity=29.3159609120521, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.4.1.83

Molecular weight: Translated: 35869; Mature: 35869

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFISVVSGCYNEEDNIRELYDRVCRVMADELPEYAFEIVIIDNASKDGTARILREIAAT
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHEEEEEEEECCCCCHHHHHHHHHHHC
DKRLKVIINNRNFGHVRSPYYGVLQAGGDAMIAMASDLEDPPEMIPQFVRKWEEGYKLVL
CCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEHHCCCCCHHHHHHHHHHHHHCCEEEE
AQKASSEESSAFSLVRKCYYFLVNRLSDVPLVKDVTGFGLYDRRVIEDMRRIGDPYPYIR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCHHHH
GMICDLGYEQFLIPFRKPLRKRGFSKNNFYTLYDLAILGITNHSKVPLRVSVMAGVGMGI
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEHHEEEEECCCCCCCEEEEHHHHCHHHH
LSFLVALAYLVYKLLFWNNFSVGTAPVVIGMFFLGAIQLFCIGMLGEYIGSIHTQVLKRP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LVIEKERINFDSGPVG
CCEEHHHCCCCCCCCC
>Mature Secondary Structure
MRFISVVSGCYNEEDNIRELYDRVCRVMADELPEYAFEIVIIDNASKDGTARILREIAAT
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHEEEEEEEECCCCCHHHHHHHHHHHC
DKRLKVIINNRNFGHVRSPYYGVLQAGGDAMIAMASDLEDPPEMIPQFVRKWEEGYKLVL
CCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEHHCCCCCHHHHHHHHHHHHHCCEEEE
AQKASSEESSAFSLVRKCYYFLVNRLSDVPLVKDVTGFGLYDRRVIEDMRRIGDPYPYIR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCHHHH
GMICDLGYEQFLIPFRKPLRKRGFSKNNFYTLYDLAILGITNHSKVPLRVSVMAGVGMGI
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEHHEEEEECCCCCCCEEEEHHHHCHHHH
LSFLVALAYLVYKLLFWNNFSVGTAPVVIGMFFLGAIQLFCIGMLGEYIGSIHTQVLKRP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LVIEKERINFDSGPVG
CCEEHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPmannose; dolichyl phosphate

Specific reaction: GDP-mannose + dolichyl phosphate = GDP + dolichyl D-mannosyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]