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The map label for this gene is mscL [H]

Identifier: 116621354

GI number: 116621354

Start: 2859928

End: 2860338

Strand: Direct

Name: mscL [H]

Synonym: Acid_2235

Alternate gene names: 116621354

Gene position: 2859928-2860338 (Clockwise)

Preceding gene: 116621353

Following gene: 116621372

Centisome position: 28.7

GC content: 57.42

Gene sequence:

>411_bases
ATGATCAAGGGGTTCAAGGAGTTTCTGTTAAAGAACAATGTGTTGGCACTGGCGGTCGCGGTGATCGTCGGCGGCGCGGT
TGGCAAGGTGGTATCCTCGCTGGCCGCGGATGTGATCATGCCAGTGATCAGCCTCGTGCTCCCGGCGGGGGATTGGCGGA
CCGCCAAGTTTGTGCTGGGCAAGACGGTGGGACCGGACGGAAAAGAAACGCTGAATACGCTGAATTACGGATCGTTCTTC
GGCAGCGTTGTAGATTTCCTGATTATTGCGCTGGTGGTCTACTTAATGACCAAGGCGCTGATCAAGGAGGCTCCGGCTCC
GGCGCCACCGCCAACGAAGAATTGCCCGCGCTGCAAGGAAGCGATCGCGATCGACGCCACGAAGTGCAAGTTCTGTACGG
CCGATGTTTAA

Upstream 100 bases:

>100_bases
GCGGCGAAGGTTCTTTCCGCATCTGCTATGCGGCGGAACGCAGTATTCTGGAACCTGCAATGGAGCGGTTGACCAAATTC
CTGCGTAAGGGAGCTCACAG

Downstream 100 bases:

>100_bases
CCGACGGGGCCCGAGTCGAAGTTGATCCGCTCCTTTTCAATGACCAGCGGGCGCTTCAGGACCTGGGTGTGAATGGAGCC
GATGTACTCGCCGAGCATAC

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MIKGFKEFLLKNNVLALAVAVIVGGAVGKVVSSLAADVIMPVISLVLPAGDWRTAKFVLGKTVGPDGKETLNTLNYGSFF
GSVVDFLIIALVVYLMTKALIKEAPAPAPPPTKNCPRCKEAIAIDATKCKFCTADV

Sequences:

>Translated_136_residues
MIKGFKEFLLKNNVLALAVAVIVGGAVGKVVSSLAADVIMPVISLVLPAGDWRTAKFVLGKTVGPDGKETLNTLNYGSFF
GSVVDFLIIALVVYLMTKALIKEAPAPAPPPTKNCPRCKEAIAIDATKCKFCTADV
>Mature_136_residues
MIKGFKEFLLKNNVLALAVAVIVGGAVGKVVSSLAADVIMPVISLVLPAGDWRTAKFVLGKTVGPDGKETLNTLNYGSFF
GSVVDFLIIALVVYLMTKALIKEAPAPAPPPTKNCPRCKEAIAIDATKCKFCTADV

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=119, Percent_Identity=40.3361344537815, Blast_Score=82, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14308; Mature: 14308

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGFKEFLLKNNVLALAVAVIVGGAVGKVVSSLAADVIMPVISLVLPAGDWRTAKFVLG
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
KTVGPDGKETLNTLNYGSFFGSVVDFLIIALVVYLMTKALIKEAPAPAPPPTKNCPRCKE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
AIAIDATKCKFCTADV
HHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIKGFKEFLLKNNVLALAVAVIVGGAVGKVVSSLAADVIMPVISLVLPAGDWRTAKFVLG
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
KTVGPDGKETLNTLNYGSFFGSVVDFLIIALVVYLMTKALIKEAPAPAPPPTKNCPRCKE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
AIAIDATKCKFCTADV
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA