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The map label for this gene is cusS [H]

Identifier: 116621284

GI number: 116621284

Start: 2770267

End: 2771811

Strand: Direct

Name: cusS [H]

Synonym: Acid_2165

Alternate gene names: 116621284

Gene position: 2770267-2771811 (Clockwise)

Preceding gene: 116621283

Following gene: 116621286

Centisome position: 27.8

GC content: 62.39

Gene sequence:

>1545_bases
GTGAGCCGGAAACGCGATTTCCGCCGATTGGCCCGCGGACTCCGCTTCCGCTTGGCGTTGAGCTACGCGCTGTTATTCAC
GTTATTGCTGACCGGCATCGCGCTTGTCTTCCGCGCCAACCTGGCGCGCGCGCTTGACACGCAAATCCAGGAAGAACTCG
CCGGAGACTGGGGTGCGATGAAGGGCTACATGCGTATCGAGCCCGATCCCGAGCTCGGCAACAAGTTCGCGGTCGCCTGG
TATTACGACAAAGACGACCCCGACGAAACCACCATCGTTCTGGACGCCCGCAAGATCTACATGGTAGCCGACCAGAACGG
CAATCCGATTCCGGCCGCGGAAACCCAGGAGGCGGCCGTCTCGACCGCCTACGAAGATATCGGCATCGATAAACCGGCGG
ATATTCGCAAGAAAGTGGCCGAGGCCTTGGCCTCCTCTAAACCCAAGGTCTTCCTGGAAGACCGCTTCACTTCGAAGGGT
GAGCCCGTCAAGATTCGCAGCGGCATCGTTTTTTCCGAGCGCCATACCGCGCCCTACTACGTCGCCATCGGAACTTCGTA
CGCGGACAACGTCAAAATTCTCCACCGCTATACCTGGATTTTCGCCGGCGTGATCCCCGGCGCCCTCATCCTCGGCTCCC
TGCTCGGATATTTCATGGCGGGCCGCGCGCTCACGCCGGTGCTGGCCGTCGCGCAGGCCGCGCAGCGCATCTCCGGGTCG
AACCTCAGTATGCGCATCCCAACGCGTGAGGCCGGCGACGAGCTCGACTATCTCATCCTCACCTTCAACCGCATGATTGA
GCGCCTGGAGGCCAGCTTCCAGCAGATCCGCCAGTTCTCCACCGACGTCTCCCACGAGCTGCGCACCCCGATCACCGGCA
TCCGCGGACAGTTGGAAGTGGCGCTTTTCACCGCCCAGACCACTGACCAGTATCGCGAGGCCATGTTCAATTCCCTGCAG
GATATCGACCGCCTCTCGCAGATTGTGCGCGCGCTGTTGCTGTTGTCGCAGGCCGAATCCGGCCAGCTGCTGCTGCAGAA
ATCGCGCCTCAATCTGTGCGAGGTGGTGGAAGACTTGGTGGATCAGTTCCAGATTCCGGCAGAAGCTGCCGGCGTACGGC
TCACCGCGTCGCTGCCGCCGCAATGCGACGCCGATGTGGATCGCGTCCAGATCGAGCGCATGATCACCAACCTGCTCTCC
AACGCCCTCAAGTTCACCCCCGAAGGCGGCGAGGTCAATGTCGCCCTGGTGGTTTATCCGGACGCCGTGGAACTCGTGGT
GCAGGACACCGGCCGCGGGATTCCAACCGAGCACTTGCCCCACATCTTCGACCGCTTCTATCGCGTACCCGGCAAGGGTA
CCGCGCCCAGCCCCGAGCAGGGCCTCGGTTTGGGCCTGAGTTTCGTGGCCTGGATCGTCAAAGCGCACCACGGGAAAATC
GATGTCGATAGCACGCCCGGCCGCGGCACCACCTTCACCATCCGTCTGCCCAACGAAGAAGTTGCGTCCGACACCCTCGA
ACTAGCCGGCCCGGCCACCTACTAG

Upstream 100 bases:

>100_bases
AAGCTGATTCAAACCGTGCGCGGCATCGGATACATGATCGAAGCGCCCGACAAACCAGCCGACCGCTCTACCGAGCCGGC
TGCCCTCCCGCAGGTCTGAT

Downstream 100 bases:

>100_bases
TCCCTCGATCGTCTGCTCTGTTTGCTCGCACGCCGCGAAGGTTCGCTTCAGAGCCGCACTACACTAGGGAGCGATCTCGC
AGTACGCGCAAACCATGCTT

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSRKRDFRRLARGLRFRLALSYALLFTLLLTGIALVFRANLARALDTQIQEELAGDWGAMKGYMRIEPDPELGNKFAVAW
YYDKDDPDETTIVLDARKIYMVADQNGNPIPAAETQEAAVSTAYEDIGIDKPADIRKKVAEALASSKPKVFLEDRFTSKG
EPVKIRSGIVFSERHTAPYYVAIGTSYADNVKILHRYTWIFAGVIPGALILGSLLGYFMAGRALTPVLAVAQAAQRISGS
NLSMRIPTREAGDELDYLILTFNRMIERLEASFQQIRQFSTDVSHELRTPITGIRGQLEVALFTAQTTDQYREAMFNSLQ
DIDRLSQIVRALLLLSQAESGQLLLQKSRLNLCEVVEDLVDQFQIPAEAAGVRLTASLPPQCDADVDRVQIERMITNLLS
NALKFTPEGGEVNVALVVYPDAVELVVQDTGRGIPTEHLPHIFDRFYRVPGKGTAPSPEQGLGLGLSFVAWIVKAHHGKI
DVDSTPGRGTTFTIRLPNEEVASDTLELAGPATY

Sequences:

>Translated_514_residues
MSRKRDFRRLARGLRFRLALSYALLFTLLLTGIALVFRANLARALDTQIQEELAGDWGAMKGYMRIEPDPELGNKFAVAW
YYDKDDPDETTIVLDARKIYMVADQNGNPIPAAETQEAAVSTAYEDIGIDKPADIRKKVAEALASSKPKVFLEDRFTSKG
EPVKIRSGIVFSERHTAPYYVAIGTSYADNVKILHRYTWIFAGVIPGALILGSLLGYFMAGRALTPVLAVAQAAQRISGS
NLSMRIPTREAGDELDYLILTFNRMIERLEASFQQIRQFSTDVSHELRTPITGIRGQLEVALFTAQTTDQYREAMFNSLQ
DIDRLSQIVRALLLLSQAESGQLLLQKSRLNLCEVVEDLVDQFQIPAEAAGVRLTASLPPQCDADVDRVQIERMITNLLS
NALKFTPEGGEVNVALVVYPDAVELVVQDTGRGIPTEHLPHIFDRFYRVPGKGTAPSPEQGLGLGLSFVAWIVKAHHGKI
DVDSTPGRGTTFTIRLPNEEVASDTLELAGPATY
>Mature_513_residues
SRKRDFRRLARGLRFRLALSYALLFTLLLTGIALVFRANLARALDTQIQEELAGDWGAMKGYMRIEPDPELGNKFAVAWY
YDKDDPDETTIVLDARKIYMVADQNGNPIPAAETQEAAVSTAYEDIGIDKPADIRKKVAEALASSKPKVFLEDRFTSKGE
PVKIRSGIVFSERHTAPYYVAIGTSYADNVKILHRYTWIFAGVIPGALILGSLLGYFMAGRALTPVLAVAQAAQRISGSN
LSMRIPTREAGDELDYLILTFNRMIERLEASFQQIRQFSTDVSHELRTPITGIRGQLEVALFTAQTTDQYREAMFNSLQD
IDRLSQIVRALLLLSQAESGQLLLQKSRLNLCEVVEDLVDQFQIPAEAAGVRLTASLPPQCDADVDRVQIERMITNLLSN
ALKFTPEGGEVNVALVVYPDAVELVVQDTGRGIPTEHLPHIFDRFYRVPGKGTAPSPEQGLGLGLSFVAWIVKAHHGKID
VDSTPGRGTTFTIRLPNEEVASDTLELAGPATY

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=324, Percent_Identity=33.9506172839506, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788393, Length=283, Percent_Identity=32.8621908127208, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1786600, Length=232, Percent_Identity=30.1724137931034, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788279, Length=246, Percent_Identity=26.0162601626016, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=32.6446280991736, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=30.2631578947368, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=30.6201550387597, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1789403, Length=319, Percent_Identity=27.5862068965517, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=30.1801801801802, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994928, Length=246, Percent_Identity=29.6747967479675, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790346, Length=275, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787894, Length=273, Percent_Identity=28.9377289377289, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789149, Length=243, Percent_Identity=28.8065843621399, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1790861, Length=259, Percent_Identity=29.3436293436293, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790551, Length=311, Percent_Identity=25.4019292604502, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=31.0810810810811, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=262, Percent_Identity=28.2442748091603, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787374, Length=284, Percent_Identity=23.943661971831, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789808, Length=257, Percent_Identity=28.0155642023346, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790300, Length=243, Percent_Identity=29.2181069958848, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56857; Mature: 56726

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKRDFRRLARGLRFRLALSYALLFTLLLTGIALVFRANLARALDTQIQEELAGDWGAM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KGYMRIEPDPELGNKFAVAWYYDKDDPDETTIVLDARKIYMVADQNGNPIPAAETQEAAV
CCCEEECCCCCCCCEEEEEEEECCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCHHHHH
STAYEDIGIDKPADIRKKVAEALASSKPKVFLEDRFTSKGEPVKIRSGIVFSERHTAPYY
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCEEEECCCCCCEE
VAIGTSYADNVKILHRYTWIFAGVIPGALILGSLLGYFMAGRALTPVLAVAQAAQRISGS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLSMRIPTREAGDELDYLILTFNRMIERLEASFQQIRQFSTDVSHELRTPITGIRGQLEV
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
ALFTAQTTDQYREAMFNSLQDIDRLSQIVRALLLLSQAESGQLLLQKSRLNLCEVVEDLV
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHH
DQFQIPAEAAGVRLTASLPPQCDADVDRVQIERMITNLLSNALKFTPEGGEVNVALVVYP
HHHCCCHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEC
DAVELVVQDTGRGIPTEHLPHIFDRFYRVPGKGTAPSPEQGLGLGLSFVAWIVKAHHGKI
CCEEEHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
DVDSTPGRGTTFTIRLPNEEVASDTLELAGPATY
ECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRKRDFRRLARGLRFRLALSYALLFTLLLTGIALVFRANLARALDTQIQEELAGDWGAM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KGYMRIEPDPELGNKFAVAWYYDKDDPDETTIVLDARKIYMVADQNGNPIPAAETQEAAV
CCCEEECCCCCCCCEEEEEEEECCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCHHHHH
STAYEDIGIDKPADIRKKVAEALASSKPKVFLEDRFTSKGEPVKIRSGIVFSERHTAPYY
HHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCEEEECCCCCCEE
VAIGTSYADNVKILHRYTWIFAGVIPGALILGSLLGYFMAGRALTPVLAVAQAAQRISGS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLSMRIPTREAGDELDYLILTFNRMIERLEASFQQIRQFSTDVSHELRTPITGIRGQLEV
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
ALFTAQTTDQYREAMFNSLQDIDRLSQIVRALLLLSQAESGQLLLQKSRLNLCEVVEDLV
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHH
DQFQIPAEAAGVRLTASLPPQCDADVDRVQIERMITNLLSNALKFTPEGGEVNVALVVYP
HHHCCCHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEC
DAVELVVQDTGRGIPTEHLPHIFDRFYRVPGKGTAPSPEQGLGLGLSFVAWIVKAHHGKI
CCEEEHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
DVDSTPGRGTTFTIRLPNEEVASDTLELAGPATY
ECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]