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The map label for this gene is rhmD [H]

Identifier: 116621176

GI number: 116621176

Start: 2612348

End: 2613466

Strand: Direct

Name: rhmD [H]

Synonym: Acid_2057

Alternate gene names: 116621176

Gene position: 2612348-2613466 (Clockwise)

Preceding gene: 116621169

Following gene: 116621177

Centisome position: 26.21

GC content: 58.09

Gene sequence:

>1119_bases
GTGGAACCCGCATCTGCTCCCAAAGTAGAGCGGTTGGAGGTGTCGGCTTATACGGTCCCCACCGAAACGCCCGAGTCGGA
CGGCACTATTGCATGGAGCGAGACCACACTAGTGATGGTGCGCATGGCGGCATGCGGGGCGGAAGGTTTGGGATACACTT
TTGCCGACGTCGCGACTGCCGCTCTGATTGATGCGCATCTTAAAGATGCGGTCGTCGGCCATAACCCTTTGAACATTCCT
GAACTTTGGTCCACGATGGTCCGCAAGGTTCGCAATTTAGGCCGGCCCGGAGTGTGCTCTATGGCAATTTCCGCCGTCGA
TAATGCTCTCTGGGATCTGAAGGCTCGTCTTCTTCATCTCTCCGTCGCCGACCTTTTGGGTCGTGCACATGACTCCATCG
GGGCTTATGGCAGCGGCGGCTTTACATCCTACTCCGATGAGGAACTAAGACAGCAGTTGACTGGGTGGATTGGCCAAGGG
CTGACTCAGGTCAAAATGAAAATCGGCAGCGATCCGGCATCGGACTTGGCGCGCGTGCGATCGGCTCGCGGCGCCATCGG
CCCGGACGCCGAATTGTTCGTAGATGCCAACGGAGCTTACGATCGCAAACAGGCGCTTGCGAAGGCCGCCGCATTCGCCG
ATCTCGGCGTGAGCTGGTTTGAAGAGCCGGTGTCCTCCGACGATCTCGCAGGTTTGCGGCTGATCCGCGAGCGCGCGCCG
GCGGGCATGCGCATTGCGGCCGGCGAATACGGTTACGATTCATTCTATTTTCGGCGCATGCTCGAAGCGGAGGCGGTGGA
CGTGCTCCAAGGCGACGCCACTCGCTGCGCGGGCATTACCGGTTTCCTTGCCGCAGGCAATTTGAGCACGTCCTTTTCAA
CGCCATTCTCTGCCCACACCGCGCCTTCCCTACACGCCCACGCAGCTTGCGCCCTTACCTCCGCAATTAACGTCGAGTAT
TTTTTCGACCATTACAGGATCGAACAGATGTTCTTCGACGGCGCTCTTGTTCCCTCGAACGGCGTGCTCCGTCCCGATTC
AACGCGTCCCGGTTTCGGTCTGGAATGCAAATTATCAGACATGGAGAAGTATCGGGTTTATGGAAACGTTACTACATGA

Upstream 100 bases:

>100_bases
GGATTCGCGGCAGGCTAAAATTGCGCTGCTGAGCACCATCGCATTCGCGGGCGACTGTTGAACGCGAAACACGCAGCGAA
GCCGTCTCATCTACCTAATA

Downstream 100 bases:

>100_bases
CTCACAGATCGCTTGGGCACGACCGCGGTCGACGGAGTATCCTTCGACTACGGCCAAAGCCCTTGAAAGCGAGCTCCTGA
AACGAATTGAGGGAGAGGTC

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEPASAPKVERLEVSAYTVPTETPESDGTIAWSETTLVMVRMAACGAEGLGYTFADVATAALIDAHLKDAVVGHNPLNIP
ELWSTMVRKVRNLGRPGVCSMAISAVDNALWDLKARLLHLSVADLLGRAHDSIGAYGSGGFTSYSDEELRQQLTGWIGQG
LTQVKMKIGSDPASDLARVRSARGAIGPDAELFVDANGAYDRKQALAKAAAFADLGVSWFEEPVSSDDLAGLRLIRERAP
AGMRIAAGEYGYDSFYFRRMLEAEAVDVLQGDATRCAGITGFLAAGNLSTSFSTPFSAHTAPSLHAHAACALTSAINVEY
FFDHYRIEQMFFDGALVPSNGVLRPDSTRPGFGLECKLSDMEKYRVYGNVTT

Sequences:

>Translated_372_residues
MEPASAPKVERLEVSAYTVPTETPESDGTIAWSETTLVMVRMAACGAEGLGYTFADVATAALIDAHLKDAVVGHNPLNIP
ELWSTMVRKVRNLGRPGVCSMAISAVDNALWDLKARLLHLSVADLLGRAHDSIGAYGSGGFTSYSDEELRQQLTGWIGQG
LTQVKMKIGSDPASDLARVRSARGAIGPDAELFVDANGAYDRKQALAKAAAFADLGVSWFEEPVSSDDLAGLRLIRERAP
AGMRIAAGEYGYDSFYFRRMLEAEAVDVLQGDATRCAGITGFLAAGNLSTSFSTPFSAHTAPSLHAHAACALTSAINVEY
FFDHYRIEQMFFDGALVPSNGVLRPDSTRPGFGLECKLSDMEKYRVYGNVTT
>Mature_372_residues
MEPASAPKVERLEVSAYTVPTETPESDGTIAWSETTLVMVRMAACGAEGLGYTFADVATAALIDAHLKDAVVGHNPLNIP
ELWSTMVRKVRNLGRPGVCSMAISAVDNALWDLKARLLHLSVADLLGRAHDSIGAYGSGGFTSYSDEELRQQLTGWIGQG
LTQVKMKIGSDPASDLARVRSARGAIGPDAELFVDANGAYDRKQALAKAAAFADLGVSWFEEPVSSDDLAGLRLIRERAP
AGMRIAAGEYGYDSFYFRRMLEAEAVDVLQGDATRCAGITGFLAAGNLSTSFSTPFSAHTAPSLHAHAACALTSAINVEY
FFDHYRIEQMFFDGALVPSNGVLRPDSTRPGFGLECKLSDMEKYRVYGNVTT

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI186972148, Length=347, Percent_Identity=23.9193083573487, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI42544119, Length=243, Percent_Identity=26.3374485596708, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI42544117, Length=242, Percent_Identity=23.9669421487603, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI226510960, Length=331, Percent_Identity=26.5861027190332, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI48994953, Length=316, Percent_Identity=26.5822784810127, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 39915; Mature: 39915

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPASAPKVERLEVSAYTVPTETPESDGTIAWSETTLVMVRMAACGAEGLGYTFADVATA
CCCCCCCCCEEEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHH
ALIDAHLKDAVVGHNPLNIPELWSTMVRKVRNLGRPGVCSMAISAVDNALWDLKARLLHL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVADLLGRAHDSIGAYGSGGFTSYSDEELRQQLTGWIGQGLTQVKMKIGSDPASDLARVR
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHH
SARGAIGPDAELFVDANGAYDRKQALAKAAAFADLGVSWFEEPVSSDDLAGLRLIRERAP
HHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHCC
AGMRIAAGEYGYDSFYFRRMLEAEAVDVLQGDATRCAGITGFLAAGNLSTSFSTPFSAHT
CCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHEEECCCCCCCCCCCCCCC
APSLHAHAACALTSAINVEYFFDHYRIEQMFFDGALVPSNGVLRPDSTRPGFGLECKLSD
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEECC
MEKYRVYGNVTT
CEEEEEEECCCC
>Mature Secondary Structure
MEPASAPKVERLEVSAYTVPTETPESDGTIAWSETTLVMVRMAACGAEGLGYTFADVATA
CCCCCCCCCEEEEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHH
ALIDAHLKDAVVGHNPLNIPELWSTMVRKVRNLGRPGVCSMAISAVDNALWDLKARLLHL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SVADLLGRAHDSIGAYGSGGFTSYSDEELRQQLTGWIGQGLTQVKMKIGSDPASDLARVR
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHH
SARGAIGPDAELFVDANGAYDRKQALAKAAAFADLGVSWFEEPVSSDDLAGLRLIRERAP
HHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHCC
AGMRIAAGEYGYDSFYFRRMLEAEAVDVLQGDATRCAGITGFLAAGNLSTSFSTPFSAHT
CCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHEEECCCCCCCCCCCCCCC
APSLHAHAACALTSAINVEYFFDHYRIEQMFFDGALVPSNGVLRPDSTRPGFGLECKLSD
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEECC
MEKYRVYGNVTT
CEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA