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The map label for this gene is galE [C]

Identifier: 116621061

GI number: 116621061

Start: 2421606

End: 2422676

Strand: Reverse

Name: galE [C]

Synonym: Acid_1942

Alternate gene names: 116621061

Gene position: 2422676-2421606 (Counterclockwise)

Preceding gene: 116621062

Following gene: 116621060

Centisome position: 24.31

GC content: 61.62

Gene sequence:

>1071_bases
ATGTCTCAGGTCGTTTCACCGCCGCGATTGACCTTCAGAACGCCCACGACCATTCCTTCAGCAAGCGTTCGACAATGGCT
TTCTTGTCGTACTATGGAAATCTCTCTGATGCGCGTCGTTATCATCGGTGGCTCCGGACATATCGGCTCGTATCTAACCC
CCCGCCTGGTGGAAGCCGGGCACTCCGTTTTTTGCGTTAGCCGCGGTTTGAAGCAGCCGTACGTGGAGCACGTGGCTTGG
AAGAGGGTCGAGTCGGTTGTCCTCGATCGGTCGGTTGAGGAAGCGGCTGGCACGTTCGGCGGAAAGATTCGCGACCTGGA
GCCCGATTGCGTCATCGATCTCACAGCATACACGCGGGAGAGCACGGAACAGCTCGTAGAGGCGTTGCGGGGGCGCGTAC
AGCAATTTCTCCATTGCGGCACGATTTGGGTGCACGGTCCCACCGTAGAGGCGCCGGTTGCCGAGGATCAGCCGCGTCGA
CCGATTAGCGAGTATGGAGTCCGCAAAGCCGAGATCGAAGCATACCTCCTCGAGGAGGCGCGGCGCCATGAATTTCCCGC
CACCGTGCTCCACCCGGGGCACCTGGTGGGAATGGGCTGGGTTCCATTGAATCCGGCGGCGAATTTCAACCCCGCGGTGT
TCGCGGATCTCGCGGCAGGGCGCGAGATCGTGCTGCCCAATTTGGGCCGCGAGACACTGCATCACGTACACGCAGACGAC
GTCGCGCAGGCTTTTGTTCAAGCCATGGACCACTGGCCCGCAGCGGTGGGGGAGTGTTTTCACGTGGTCTCTCCTGCCGC
CGTGTCGATGGCCGGTTACGCCGAGGCTGTCGCCGGCTGGTTCGGGCAGCGGGCGCGCATACGGTTCCTGCCATGGGAGG
AGTGGCGGTCGGTCGTATCGGAGAAGGAGGCGAAGATCACCTGGGATCACATCGCCAGATCGCCTGTTTGCAGCATTCGC
AAGGCGGAACAGCTGCTGAACTACCGGCCGCGGTACAGGTCGCTCGAAGCGGTGCGGGAATCCGTGTTGTGGCTGGTGGC
AAACCTCATCATCCGAGGCGCAAGTGTCTGA

Upstream 100 bases:

>100_bases
CATACAGAATCTGCCCAGTGATGAACTCAGACGCGGTAACACCAGGAACAGCGAGACCGAACGTATTTTCGATAAATGAC
ACTCGCGGATTCCAGCGTAG

Downstream 100 bases:

>100_bases
AGCCGGGGCCGGAATGGAATAATCTAGTACAGGAATGCCGCTAACAAGTGTGAAGAAAAATGGGAAGCGGAGGGCGGGCG
CTCCGGACACCCCCGCCATA

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: Oxidoreductase; Isoflavone Reductase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSQVVSPPRLTFRTPTTIPSASVRQWLSCRTMEISLMRVVIIGGSGHIGSYLTPRLVEAGHSVFCVSRGLKQPYVEHVAW
KRVESVVLDRSVEEAAGTFGGKIRDLEPDCVIDLTAYTRESTEQLVEALRGRVQQFLHCGTIWVHGPTVEAPVAEDQPRR
PISEYGVRKAEIEAYLLEEARRHEFPATVLHPGHLVGMGWVPLNPAANFNPAVFADLAAGREIVLPNLGRETLHHVHADD
VAQAFVQAMDHWPAAVGECFHVVSPAAVSMAGYAEAVAGWFGQRARIRFLPWEEWRSVVSEKEAKITWDHIARSPVCSIR
KAEQLLNYRPRYRSLEAVRESVLWLVANLIIRGASV

Sequences:

>Translated_356_residues
MSQVVSPPRLTFRTPTTIPSASVRQWLSCRTMEISLMRVVIIGGSGHIGSYLTPRLVEAGHSVFCVSRGLKQPYVEHVAW
KRVESVVLDRSVEEAAGTFGGKIRDLEPDCVIDLTAYTRESTEQLVEALRGRVQQFLHCGTIWVHGPTVEAPVAEDQPRR
PISEYGVRKAEIEAYLLEEARRHEFPATVLHPGHLVGMGWVPLNPAANFNPAVFADLAAGREIVLPNLGRETLHHVHADD
VAQAFVQAMDHWPAAVGECFHVVSPAAVSMAGYAEAVAGWFGQRARIRFLPWEEWRSVVSEKEAKITWDHIARSPVCSIR
KAEQLLNYRPRYRSLEAVRESVLWLVANLIIRGASV
>Mature_355_residues
SQVVSPPRLTFRTPTTIPSASVRQWLSCRTMEISLMRVVIIGGSGHIGSYLTPRLVEAGHSVFCVSRGLKQPYVEHVAWK
RVESVVLDRSVEEAAGTFGGKIRDLEPDCVIDLTAYTRESTEQLVEALRGRVQQFLHCGTIWVHGPTVEAPVAEDQPRRP
ISEYGVRKAEIEAYLLEEARRHEFPATVLHPGHLVGMGWVPLNPAANFNPAVFADLAAGREIVLPNLGRETLHHVHADDV
AQAFVQAMDHWPAAVGECFHVVSPAAVSMAGYAEAVAGWFGQRARIRFLPWEEWRSVVSEKEAKITWDHIARSPVCSIRK
AEQLLNYRPRYRSLEAVRESVLWLVANLIIRGASV

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 39685; Mature: 39554

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVVSPPRLTFRTPTTIPSASVRQWLSCRTMEISLMRVVIIGGSGHIGSYLTPRLVEAG
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCHHHHHCC
HSVFCVSRGLKQPYVEHVAWKRVESVVLDRSVEEAAGTFGGKIRDLEPDCVIDLTAYTRE
CCEEEHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCEEEEHHHHHH
STEQLVEALRGRVQQFLHCGTIWVHGPTVEAPVAEDQPRRPISEYGVRKAEIEAYLLEEA
HHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHH
RRHEFPATVLHPGHLVGMGWVPLNPAANFNPAVFADLAAGREIVLPNLGRETLHHVHADD
HHCCCCCEEECCCEEEEECCEECCCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHCCHHH
VAQAFVQAMDHWPAAVGECFHVVSPAAVSMAGYAEAVAGWFGQRARIRFLPWEEWRSVVS
HHHHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
EKEAKITWDHIARSPVCSIRKAEQLLNYRPRYRSLEAVRESVLWLVANLIIRGASV
HHHCCEEHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQVVSPPRLTFRTPTTIPSASVRQWLSCRTMEISLMRVVIIGGSGHIGSYLTPRLVEAG
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCHHHHHCC
HSVFCVSRGLKQPYVEHVAWKRVESVVLDRSVEEAAGTFGGKIRDLEPDCVIDLTAYTRE
CCEEEHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCEEEEHHHHHH
STEQLVEALRGRVQQFLHCGTIWVHGPTVEAPVAEDQPRRPISEYGVRKAEIEAYLLEEA
HHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHH
RRHEFPATVLHPGHLVGMGWVPLNPAANFNPAVFADLAAGREIVLPNLGRETLHHVHADD
HHCCCCCEEECCCEEEEECCEECCCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHCCHHH
VAQAFVQAMDHWPAAVGECFHVVSPAAVSMAGYAEAVAGWFGQRARIRFLPWEEWRSVVS
HHHHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHH
EKEAKITWDHIARSPVCSIRKAEQLLNYRPRYRSLEAVRESVLWLVANLIIRGASV
HHHCCEEHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA