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The map label for this gene is ilvA [H]

Identifier: 116620848

GI number: 116620848

Start: 2145407

End: 2146366

Strand: Reverse

Name: ilvA [H]

Synonym: Acid_1729

Alternate gene names: 116620848

Gene position: 2146366-2145407 (Counterclockwise)

Preceding gene: 116620850

Following gene: 116620847

Centisome position: 21.54

GC content: 66.88

Gene sequence:

>960_bases
ATGAGATTGCCAACTGGGGAAGAGATCGAGCAGGCGCGAGGGGTGGTTTATGAAGCAATGCCGGCGACGGCGCAATACCG
GTGGCCGCTGGTGTGCGAGCGGGCGGGGATGGAGGTGTGGCTGAAGCACGAGAACCATACGCCGCTGGGGGCGTTCAAGG
TGCGAAGCGCGCTGGTGTATTTCCGGCGGCTGCGCGAATCGGGCACCGGCGCGCGGGTAGCGGTGACGGCGACGCGGGGG
AATTATGGACAGGGCGTTGCTTTCGCAGCGCGGCGGGAAGGGATGGAGGCGGTGATCTATGTGCCGCACGGGAACAGCGA
ATCGAAGAATCGCGCGATGCGGGCGCTGGGGGCGCAGCTGGTGGAGCACGGGGCGGATTTCGAGGAGGCGCGGCAGGAAG
CGCGGCGGTGGGCCGAGAGCGAGGGGCATCATTACGTGCCCTCGTTTCACGAATGGCTGGTGGCGGGAACGGCGACGTAT
TCGTGGGAGCTGTTCGAGGCGGTAAAGGGAATCGATGTGGCTTTCGTGCCGATCGGGATGGGGTCGGGACTGCTGGGAAT
GTGCGCGGCGCGGGAGGCGATGGGGCTGAAGACGGAGATTGTGGGCGTGACGAGCGAGCACGCGCGGGCATACTACGAAT
CGTTCATGCGGCGGGAGATGGTGATCTCGCCGGCGCGGACGAAGCTGGCGGACGGGATGGCGGTGCCGACTCCGGACGCG
GGGGCGCTGGAGCTGATGTGGCGGCACGTGTCGCGGGTGGTGACGGTGAACGACGAAGAGGTGGGTGCGGCGATGAGGGC
GCTGTTCGACGACACGCACAATATCGCGGAGGGTGGAGGCGCAGCGGGGATGGCGGCAATTCTAAAGGAGCGGGAGGGGC
TGCGCGGGAAGCGGGTGGCGACCGTGATCTCGGGTGGGAACGTGGATCGCGCGGTCTTCGCGGAAGTGTTGAGGGGGTAG

Upstream 100 bases:

>100_bases
CGACAGGGGCTGGGGTCGGTCTGGAAGAGTACGACACGGTGGGCGGGAAAAGCATTTTCTCGCCAAGAACGCAGAGATCG
CAGAGAACAAAGAGAACAAG

Downstream 100 bases:

>100_bases
GCAGATTAGATGGACAGGCCGCGAGGCCTGTCCCACGGGCGTCGATCCTCCGAGCGGGTGGGGACGCCATTACGATCGCG
GCGTTCCCTTCCCTCAGCAA

Product: hypothetical protein

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRLPTGEEIEQARGVVYEAMPATAQYRWPLVCERAGMEVWLKHENHTPLGAFKVRSALVYFRRLRESGTGARVAVTATRG
NYGQGVAFAARREGMEAVIYVPHGNSESKNRAMRALGAQLVEHGADFEEARQEARRWAESEGHHYVPSFHEWLVAGTATY
SWELFEAVKGIDVAFVPIGMGSGLLGMCAAREAMGLKTEIVGVTSEHARAYYESFMRREMVISPARTKLADGMAVPTPDA
GALELMWRHVSRVVTVNDEEVGAAMRALFDDTHNIAEGGGAAGMAAILKEREGLRGKRVATVISGGNVDRAVFAEVLRG

Sequences:

>Translated_319_residues
MRLPTGEEIEQARGVVYEAMPATAQYRWPLVCERAGMEVWLKHENHTPLGAFKVRSALVYFRRLRESGTGARVAVTATRG
NYGQGVAFAARREGMEAVIYVPHGNSESKNRAMRALGAQLVEHGADFEEARQEARRWAESEGHHYVPSFHEWLVAGTATY
SWELFEAVKGIDVAFVPIGMGSGLLGMCAAREAMGLKTEIVGVTSEHARAYYESFMRREMVISPARTKLADGMAVPTPDA
GALELMWRHVSRVVTVNDEEVGAAMRALFDDTHNIAEGGGAAGMAAILKEREGLRGKRVATVISGGNVDRAVFAEVLRG
>Mature_319_residues
MRLPTGEEIEQARGVVYEAMPATAQYRWPLVCERAGMEVWLKHENHTPLGAFKVRSALVYFRRLRESGTGARVAVTATRG
NYGQGVAFAARREGMEAVIYVPHGNSESKNRAMRALGAQLVEHGADFEEARQEARRWAESEGHHYVPSFHEWLVAGTATY
SWELFEAVKGIDVAFVPIGMGSGLLGMCAAREAMGLKTEIVGVTSEHARAYYESFMRREMVISPARTKLADGMAVPTPDA
GALELMWRHVSRVVTVNDEEVGAAMRALFDDTHNIAEGGGAAGMAAILKEREGLRGKRVATVISGGNVDRAVFAEVLRG

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=310, Percent_Identity=25.8064516129032, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1790207, Length=311, Percent_Identity=30.2250803858521, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789505, Length=297, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789235, Length=293, Percent_Identity=23.5494880546075, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71991565, Length=298, Percent_Identity=28.5234899328859, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17537387, Length=275, Percent_Identity=32, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320930, Length=281, Percent_Identity=30.6049822064057, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322631, Length=320, Percent_Identity=27.5, Blast_Score=112, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21355833, Length=284, Percent_Identity=30.9859154929577, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24645328, Length=219, Percent_Identity=32.4200913242009, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 34877; Mature: 34877

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPTGEEIEQARGVVYEAMPATAQYRWPLVCERAGMEVWLKHENHTPLGAFKVRSALVY
CCCCCCHHHHHHCCEEEECCCCCCCCCCCHHEECCCCEEEEEECCCCCCHHHHHHHHHHH
FRRLRESGTGARVAVTATRGNYGQGVAFAARREGMEAVIYVPHGNSESKNRAMRALGAQL
HHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
VEHGADFEEARQEARRWAESEGHHYVPSFHEWLVAGTATYSWELFEAVKGIDVAFVPIGM
HHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCC
GSGLLGMCAAREAMGLKTEIVGVTSEHARAYYESFMRREMVISPARTKLADGMAVPTPDA
CCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCC
GALELMWRHVSRVVTVNDEEVGAAMRALFDDTHNIAEGGGAAGMAAILKEREGLRGKRVA
HHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEE
TVISGGNVDRAVFAEVLRG
EEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MRLPTGEEIEQARGVVYEAMPATAQYRWPLVCERAGMEVWLKHENHTPLGAFKVRSALVY
CCCCCCHHHHHHCCEEEECCCCCCCCCCCHHEECCCCEEEEEECCCCCCHHHHHHHHHHH
FRRLRESGTGARVAVTATRGNYGQGVAFAARREGMEAVIYVPHGNSESKNRAMRALGAQL
HHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
VEHGADFEEARQEARRWAESEGHHYVPSFHEWLVAGTATYSWELFEAVKGIDVAFVPIGM
HHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCC
GSGLLGMCAAREAMGLKTEIVGVTSEHARAYYESFMRREMVISPARTKLADGMAVPTPDA
CCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCC
GALELMWRHVSRVVTVNDEEVGAAMRALFDDTHNIAEGGGAAGMAAILKEREGLRGKRVA
HHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEE
TVISGGNVDRAVFAEVLRG
EEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]