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The map label for this gene is hsdS [H]

Identifier: 116620728

GI number: 116620728

Start: 1983398

End: 1983907

Strand: Reverse

Name: hsdS [H]

Synonym: Acid_1608

Alternate gene names: 116620728

Gene position: 1983907-1983398 (Counterclockwise)

Preceding gene: 116620733

Following gene: 116620724

Centisome position: 19.91

GC content: 55.1

Gene sequence:

>510_bases
TTGACAGCGCTAGCGATCTTCAGACCAATCAGGTCCGTGTCTGCGCAATTCTTGAAATACGCTTTGCTCGAGGGTGAATC
GCTGCGTAGAATCATCATGGAAACCCGTGGAATCGTTGGACAGAGCAATATTTCACTCGAGCAATGCCGTTCATTAATCG
TTTCTGTACCCTCTTCACAAGAACAACGAGAAATCATCCGCCGAGTCGAGGCCTTCTTTGCTCTGGCGGACCGGCTTGAG
GCGCGCTGCACCAACGCCAAGGCTCATGTCGACAAGCTGACCCAATCAATCCTCTCGAAAGCATTCCGTGGCCAACTGGT
GGAGACTGAGGCGGCACTCGCCGAAGCGGAAAGGCGAGAGTTTGAGTCTGCTGCCGAACTCCTTGCGCGCCTAAATGGGA
CACCAACACGAGGCCTGAAGCGCTCGACCCCTGAGCCAACGAATGGCTCGGGCACGCACAATGACCACCGGAACATGACA
CGAAAGAGAAAGGCTGCGAAGCGAGGCTGA

Upstream 100 bases:

>100_bases
CTAGACCGCTCGAATATCTTCAGCTTACGCGGTCATCGCATCGCGAAGCCGTCTGTTATCTGCGGCCACATCTTCGTCCA
GGATTGAAAACAGAGGAGTT

Downstream 100 bases:

>100_bases
AACGCTGCTGAGGAATCGACAGCTACAAAATCGACTACAGCGCCAAGTTACGAGCCAAGGATCTCGGCTGTAACCTATTG
ATTCTATTGGTCGGGGCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: S.EcoBI; Type I restriction enzyme EcoBI specificity protein; S protein [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MTALAIFRPIRSVSAQFLKYALLEGESLRRIIMETRGIVGQSNISLEQCRSLIVSVPSSQEQREIIRRVEAFFALADRLE
ARCTNAKAHVDKLTQSILSKAFRGQLVETEAALAEAERREFESAAELLARLNGTPTRGLKRSTPEPTNGSGTHNDHRNMT
RKRKAAKRG

Sequences:

>Translated_169_residues
MTALAIFRPIRSVSAQFLKYALLEGESLRRIIMETRGIVGQSNISLEQCRSLIVSVPSSQEQREIIRRVEAFFALADRLE
ARCTNAKAHVDKLTQSILSKAFRGQLVETEAALAEAERREFESAAELLARLNGTPTRGLKRSTPEPTNGSGTHNDHRNMT
RKRKAAKRG
>Mature_168_residues
TALAIFRPIRSVSAQFLKYALLEGESLRRIIMETRGIVGQSNISLEQCRSLIVSVPSSQEQREIIRRVEAFFALADRLEA
RCTNAKAHVDKLTQSILSKAFRGQLVETEAALAEAERREFESAAELLARLNGTPTRGLKRSTPEPTNGSGTHNDHRNMTR
KRKAAKRG

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=67, Percent_Identity=53.7313432835821, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 18875; Mature: 18744

Theoretical pI: Translated: 11.09; Mature: 11.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALAIFRPIRSVSAQFLKYALLEGESLRRIIMETRGIVGQSNISLEQCRSLIVSVPSSQ
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCH
EQREIIRRVEAFFALADRLEARCTNAKAHVDKLTQSILSKAFRGQLVETEAALAEAERRE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FESAAELLARLNGTPTRGLKRSTPEPTNGSGTHNDHRNMTRKRKAAKRG
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TALAIFRPIRSVSAQFLKYALLEGESLRRIIMETRGIVGQSNISLEQCRSLIVSVPSSQ
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCH
EQREIIRRVEAFFALADRLEARCTNAKAHVDKLTQSILSKAFRGQLVETEAALAEAERRE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FESAAELLARLNGTPTRGLKRSTPEPTNGSGTHNDHRNMTRKRKAAKRG
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6304321 [H]