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The map label for this gene is prfB [H]

Identifier: 116620705

GI number: 116620705

Start: 1944972

End: 1945940

Strand: Reverse

Name: prfB [H]

Synonym: Acid_1585

Alternate gene names: 116620705

Gene position: 1945940-1944972 (Counterclockwise)

Preceding gene: 116620706

Following gene: 116620704

Centisome position: 19.53

GC content: 62.02

Gene sequence:

>969_bases
ATGCAGGATCGCAAGCGGCTGGAAGAGGCCGTTGCGAACGACGATCAAATCCGCGCCCTGACCGACGACCTGGACACGAT
GTTCGAACTGGCGCGCGAAGGCGAGCCCGTCCAGGCCGATATCGAACGCGACCTCAAACCCTACAGCGAACTGCTCGAGC
GCCTCGAAACCGCCATGCTCCTCAGCGGCGAAAACGATGCGCGCAGCGCCATCGTCACCATCCACCCCGGCGCCGGCGGC
ACCGAAAGTCAGGACTGGGCCGAGATGCTGCTGCGCATGTACCTCCGCTGGGCCGAGCGCGAGAAGTTCGCCACCGTGAT
TACAGACCGCCTGGAAGGGGAAGGCGCCGGCATCAAGAGCGTCACCTTCGAGGTCAACGGCGAAAACGCATACGGCCTGC
TGCAGTCGGAAATCGGCGTCCATCGCCTCGTGCGAATTTCACCCTTCGACGCCAATGCGCGGCGCCACACCTCCTTCGCC
AGCGTCTTCGTGTTTCCGCAGGTCGATGACGAAATCAAGATCGATATTCAGGATAAGGATCTGCGCATCGACACCTTCCG
CGCATCGGGCGCCGGCGGCCAGCACGTCAACATGACGGATTCGGCCGTGCGCATCACCCACTTCCCCACCGGAATCGTCG
TCCAGTGCCAGAACGAACGTTCTCAGCACAAGAACCGCGCCACTGCCATGAAGCAGCTGCGCGCCTGTCTTTACGAGCAC
GAGCTCGAAAAGAAGCGGGCGGAGACCCGCGAGACCGAAGATTCCAAGCTGGAGATTAATTTCGGCAGCCAGATTCGTAA
CTACGTGCTCGCGCCCTACCGGCTGGTGAAAGACCTGCGCAGCAAGCTCGCCCTCGGCGATGTGGACCGCGTGCTCGAAG
GCGGACTCGACCCGCTGATTCACTCCTACCTGGTGTGGCGCAAAACCGGCAAGATCGCGGGCGATGATAAAGACGAGTCG
CCAGAGTGA

Upstream 100 bases:

>100_bases
TATCTTTGACGCTCCTGCACAGAAGCAGAAGCTAGCCAAAACCGAAGAACAGATCTCGGCCCCGGATTTCTGGAGCCAGC
CCGAAAAAAGCCAGAAAGTC

Downstream 100 bases:

>100_bases
CACCCGAAGCCAACATGGCGTATGCCGCCGAGCTGATCCGCGGCGGCAAACTCGTTGCCTTCCCCACCGAGACCGTCTAC
GGACTCGGCGCCAACGCGCT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQDRKRLEEAVANDDQIRALTDDLDTMFELAREGEPVQADIERDLKPYSELLERLETAMLLSGENDARSAIVTIHPGAGG
TESQDWAEMLLRMYLRWAEREKFATVITDRLEGEGAGIKSVTFEVNGENAYGLLQSEIGVHRLVRISPFDANARRHTSFA
SVFVFPQVDDEIKIDIQDKDLRIDTFRASGAGGQHVNMTDSAVRITHFPTGIVVQCQNERSQHKNRATAMKQLRACLYEH
ELEKKRAETRETEDSKLEINFGSQIRNYVLAPYRLVKDLRSKLALGDVDRVLEGGLDPLIHSYLVWRKTGKIAGDDKDES
PE

Sequences:

>Translated_322_residues
MQDRKRLEEAVANDDQIRALTDDLDTMFELAREGEPVQADIERDLKPYSELLERLETAMLLSGENDARSAIVTIHPGAGG
TESQDWAEMLLRMYLRWAEREKFATVITDRLEGEGAGIKSVTFEVNGENAYGLLQSEIGVHRLVRISPFDANARRHTSFA
SVFVFPQVDDEIKIDIQDKDLRIDTFRASGAGGQHVNMTDSAVRITHFPTGIVVQCQNERSQHKNRATAMKQLRACLYEH
ELEKKRAETRETEDSKLEINFGSQIRNYVLAPYRLVKDLRSKLALGDVDRVLEGGLDPLIHSYLVWRKTGKIAGDDKDES
PE
>Mature_322_residues
MQDRKRLEEAVANDDQIRALTDDLDTMFELAREGEPVQADIERDLKPYSELLERLETAMLLSGENDARSAIVTIHPGAGG
TESQDWAEMLLRMYLRWAEREKFATVITDRLEGEGAGIKSVTFEVNGENAYGLLQSEIGVHRLVRISPFDANARRHTSFA
SVFVFPQVDDEIKIDIQDKDLRIDTFRASGAGGQHVNMTDSAVRITHFPTGIVVQCQNERSQHKNRATAMKQLRACLYEH
ELEKKRAETRETEDSKLEINFGSQIRNYVLAPYRLVKDLRSKLALGDVDRVLEGGLDPLIHSYLVWRKTGKIAGDDKDES
PE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=264, Percent_Identity=37.5, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI34577120, Length=267, Percent_Identity=35.9550561797753, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI166795305, Length=165, Percent_Identity=37.5757575757576, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI2367172, Length=305, Percent_Identity=53.1147540983606, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1787462, Length=250, Percent_Identity=41.6, Blast_Score=184, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=291, Percent_Identity=31.6151202749141, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=303, Percent_Identity=35.6435643564356, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19921226, Length=304, Percent_Identity=37.171052631579, Blast_Score=161, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36471; Mature: 36471

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRKRLEEAVANDDQIRALTDDLDTMFELAREGEPVQADIERDLKPYSELLERLETAML
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHH
LSGENDARSAIVTIHPGAGGTESQDWAEMLLRMYLRWAEREKFATVITDRLEGEGAGIKS
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
VTFEVNGENAYGLLQSEIGVHRLVRISPFDANARRHTSFASVFVFPQVDDEIKIDIQDKD
EEEEECCCCCHHHHHHHHCHHHEEEECCCCCCCHHCCCEEEEEEEECCCCCEEEEEECCC
LRIDTFRASGAGGQHVNMTDSAVRITHFPTGIVVQCQNERSQHKNRATAMKQLRACLYEH
EEEEEEECCCCCCCEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
ELEKKRAETRETEDSKLEINFGSQIRNYVLAPYRLVKDLRSKLALGDVDRVLEGGLDPLI
HHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHH
HSYLVWRKTGKIAGDDKDESPE
HHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQDRKRLEEAVANDDQIRALTDDLDTMFELAREGEPVQADIERDLKPYSELLERLETAML
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHH
LSGENDARSAIVTIHPGAGGTESQDWAEMLLRMYLRWAEREKFATVITDRLEGEGAGIKS
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
VTFEVNGENAYGLLQSEIGVHRLVRISPFDANARRHTSFASVFVFPQVDDEIKIDIQDKD
EEEEECCCCCHHHHHHHHCHHHEEEECCCCCCCHHCCCEEEEEEEECCCCCEEEEEECCC
LRIDTFRASGAGGQHVNMTDSAVRITHFPTGIVVQCQNERSQHKNRATAMKQLRACLYEH
EEEEEEECCCCCCCEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
ELEKKRAETRETEDSKLEINFGSQIRNYVLAPYRLVKDLRSKLALGDVDRVLEGGLDPLI
HHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHH
HSYLVWRKTGKIAGDDKDESPE
HHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA