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The map label for this gene is hisS

Identifier: 116620657

GI number: 116620657

Start: 1887386

End: 1888630

Strand: Direct

Name: hisS

Synonym: Acid_1537

Alternate gene names: 116620657

Gene position: 1887386-1888630 (Clockwise)

Preceding gene: 116620654

Following gene: 116620658

Centisome position: 18.94

GC content: 61.53

Gene sequence:

>1245_bases
TTGATAAAAGCCGTCAAAGGAACCAGAGATTTACTGCCCCCGTCCACCGAAGTCTGGAACAGGGTGGAACAGGTGGCGCG
CGCCATTTTTCGCGCCTACAACTACCACGAGATCCGCACGCCGATCTTCGAGGAGACGCAGCTCTTCTCCCGCGGCGTAG
GCACGGAAACCGACATCGTCACGAAGGAAATGTACACCTTCGAAGATCGTGATGGCAGTTCACTCACGCTCCGTCCGGAA
AATACCGCGTCGGTCATCCGCGCCTATATCGAGCACCGTCTGGACCAGCGTCCCGGCGTGCAGAAGCTCTATTACATCGG
TCCGATGTTCCGCCGCGAGCGGCCGCAGAAGGGCCGCTATCGCCAATTCTTCCAGATCGGCGCGGAAGCCATCGGCTCGG
AATCTCCCGTGGTGGACGCCGAAGTCATCGAACTGGTAGTCGAAATCCTCAAAGGCGTTGGTCTCAGCGGCTTCAAGCTG
CTGATCAACTCCGTGGGCGATCACAACTGCCGTCCGCAGTACGTAGAGAAGTTGAAGGCCGAACTCAAGGACAAGGCTGC
CGGCATGTGCGGCGATTGCCAGCGCCGCGCCGAAACCAATCCGCTGCGCGTGCTCGATTGCAAAGTCGAGGCCGACCAGC
CCATCATCGACGCCCTGCCCTCAATCGTGGACTACCTCTGTGAGCCCTGCCGCGCCCACTTCGAAGCCGTCAAGAGCTAC
TTGACCGACCGCGGCATCGAGTACGAAGTGCGTCCGCGCATGGTGCGCGGCTTGGATTACTACATGCGCACCACCTTCGA
GGTCGTGCACGGAGCGCTTGGCGCGCAGAATTCCGTGCTTGGCGGCGGCCGCTACGATGGCCTCGCCGAATCCCTGGGCT
CCCGCGTGCACGCTCCCGGAATCGGCTTTTCGATCGGTGAAGATCGCCTCGTGATGAGCGTGGAAGGCGATCAACCCGCG
TCGCAGCTCGACCTCTGCATTGCCCCGTTGGGCGAAGCTGCAGTGCGTCACGCCGCGATCCTGGCGCGCGATTTCCGGCG
CAGCGGCCATTCGGTGGAACTGGTCGAGGGCAAGCTCAAGCGTGCCATGGAACTCGCCAACAAATTCGGCGCGCGCTACA
CCCTCATCATCGGCGACAATGAAATCGCGGCCGGGCGCTATGCGCTCAAGAACATGGCCACGGGCGAACAAGTGGAATTG
ACGCGAGACGAAATTGCCGCTCGGCTCGACGCGGCCTCAAACTGA

Upstream 100 bases:

>100_bases
GAGCGATAGGCGCCGCATACGTCGATTGTAACGCCCGTTCACGAATGCAAGGATAATCCCTTATTCCGAGGCATGTTAAC
ATGAAAGTTTCCCCACTACC

Downstream 100 bases:

>100_bases
AAAGACCGATATGGAAACCTCTGTTGCACTGGATTTTCTGGGCGACCTTCGCCGCACTCACATGTGCGGCGAACTTCGCG
CTTCGAACGCGGGCCAAAAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MIKAVKGTRDLLPPSTEVWNRVEQVARAIFRAYNYHEIRTPIFEETQLFSRGVGTETDIVTKEMYTFEDRDGSSLTLRPE
NTASVIRAYIEHRLDQRPGVQKLYYIGPMFRRERPQKGRYRQFFQIGAEAIGSESPVVDAEVIELVVEILKGVGLSGFKL
LINSVGDHNCRPQYVEKLKAELKDKAAGMCGDCQRRAETNPLRVLDCKVEADQPIIDALPSIVDYLCEPCRAHFEAVKSY
LTDRGIEYEVRPRMVRGLDYYMRTTFEVVHGALGAQNSVLGGGRYDGLAESLGSRVHAPGIGFSIGEDRLVMSVEGDQPA
SQLDLCIAPLGEAAVRHAAILARDFRRSGHSVELVEGKLKRAMELANKFGARYTLIIGDNEIAAGRYALKNMATGEQVEL
TRDEIAARLDAASN

Sequences:

>Translated_414_residues
MIKAVKGTRDLLPPSTEVWNRVEQVARAIFRAYNYHEIRTPIFEETQLFSRGVGTETDIVTKEMYTFEDRDGSSLTLRPE
NTASVIRAYIEHRLDQRPGVQKLYYIGPMFRRERPQKGRYRQFFQIGAEAIGSESPVVDAEVIELVVEILKGVGLSGFKL
LINSVGDHNCRPQYVEKLKAELKDKAAGMCGDCQRRAETNPLRVLDCKVEADQPIIDALPSIVDYLCEPCRAHFEAVKSY
LTDRGIEYEVRPRMVRGLDYYMRTTFEVVHGALGAQNSVLGGGRYDGLAESLGSRVHAPGIGFSIGEDRLVMSVEGDQPA
SQLDLCIAPLGEAAVRHAAILARDFRRSGHSVELVEGKLKRAMELANKFGARYTLIIGDNEIAAGRYALKNMATGEQVEL
TRDEIAARLDAASN
>Mature_414_residues
MIKAVKGTRDLLPPSTEVWNRVEQVARAIFRAYNYHEIRTPIFEETQLFSRGVGTETDIVTKEMYTFEDRDGSSLTLRPE
NTASVIRAYIEHRLDQRPGVQKLYYIGPMFRRERPQKGRYRQFFQIGAEAIGSESPVVDAEVIELVVEILKGVGLSGFKL
LINSVGDHNCRPQYVEKLKAELKDKAAGMCGDCQRRAETNPLRVLDCKVEADQPIIDALPSIVDYLCEPCRAHFEAVKSY
LTDRGIEYEVRPRMVRGLDYYMRTTFEVVHGALGAQNSVLGGGRYDGLAESLGSRVHAPGIGFSIGEDRLVMSVEGDQPA
SQLDLCIAPLGEAAVRHAAILARDFRRSGHSVELVEGKLKRAMELANKFGARYTLIIGDNEIAAGRYALKNMATGEQVEL
TRDEIAARLDAASN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=467, Percent_Identity=23.7687366167024, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI6996014, Length=459, Percent_Identity=24.6187363834423, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788861, Length=419, Percent_Identity=43.9140811455847, Blast_Score=340, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6325290, Length=374, Percent_Identity=23.5294117647059, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24643061, Length=474, Percent_Identity=24.0506329113924, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_SOLUE (Q028M4)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_822813.1
- ProteinModelPortal:   Q028M4
- SMR:   Q028M4
- STRING:   Q028M4
- GeneID:   4431031
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1537
- NMPDR:   fig|234267.9.peg.1467
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q028M4
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 46142; Mature: 46142

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKAVKGTRDLLPPSTEVWNRVEQVARAIFRAYNYHEIRTPIFEETQLFSRGVGTETDIV
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCHHHH
TKEMYTFEDRDGSSLTLRPENTASVIRAYIEHRLDQRPGVQKLYYIGPMFRRERPQKGRY
HHHHHEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHCCCCCHHH
RQFFQIGAEAIGSESPVVDAEVIELVVEILKGVGLSGFKLLINSVGDHNCRPQYVEKLKA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
ELKDKAAGMCGDCQRRAETNPLRVLDCKVEADQPIIDALPSIVDYLCEPCRAHFEAVKSY
HHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTDRGIEYEVRPRMVRGLDYYMRTTFEVVHGALGAQNSVLGGGRYDGLAESLGSRVHAPG
HHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC
IGFSIGEDRLVMSVEGDQPASQLDLCIAPLGEAAVRHAAILARDFRRSGHSVELVEGKLK
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RAMELANKFGARYTLIIGDNEIAAGRYALKNMATGEQVELTRDEIAARLDAASN
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKAVKGTRDLLPPSTEVWNRVEQVARAIFRAYNYHEIRTPIFEETQLFSRGVGTETDIV
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCHHHH
TKEMYTFEDRDGSSLTLRPENTASVIRAYIEHRLDQRPGVQKLYYIGPMFRRERPQKGRY
HHHHHEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHCCCCCHHH
RQFFQIGAEAIGSESPVVDAEVIELVVEILKGVGLSGFKLLINSVGDHNCRPQYVEKLKA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
ELKDKAAGMCGDCQRRAETNPLRVLDCKVEADQPIIDALPSIVDYLCEPCRAHFEAVKSY
HHHHHHCCCCHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LTDRGIEYEVRPRMVRGLDYYMRTTFEVVHGALGAQNSVLGGGRYDGLAESLGSRVHAPG
HHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC
IGFSIGEDRLVMSVEGDQPASQLDLCIAPLGEAAVRHAAILARDFRRSGHSVELVEGKLK
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RAMELANKFGARYTLIIGDNEIAAGRYALKNMATGEQVELTRDEIAARLDAASN
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA