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The map label for this gene is lieA [H]

Identifier: 116620638

GI number: 116620638

Start: 1862399

End: 1863166

Strand: Direct

Name: lieA [H]

Synonym: Acid_1517

Alternate gene names: 116620638

Gene position: 1862399-1863166 (Clockwise)

Preceding gene: 116620637

Following gene: 116620640

Centisome position: 18.69

GC content: 63.54

Gene sequence:

>768_bases
TTGAGCTGGTGCGCGTGGGCGCAGCCTGCCCTCGAACAGCCGGTGATTCAGCATCGCCTGGAAAGTCTAAGCCGAAAAAT
ACCGATCCGCCGCGCCACGCTCGAGGTGCTTTTCCGCGAAGCGGGCTGCGACGGCGCGAATCTCACGGCGCAGCGCGTGC
CGGGGTCGAAGGAGCCGAACATCATCTGTACGCTGCCCGGCGACAGCCCCTCCGCGATCGTGGTCGGCGGACATTACGAC
CTGGTCGAAGAGGGCGCGGGTGCGGTGGACGATTGGAGCGGCGCCGTGCTGCTGCCCAGTCTTTACGAGACGCTCAAGCG
CACACCCCGCAAGCATACTTACGTGTTTATCGCCTTCGCAGCCGAAGAGGTGGGGCTGCATGGTTCGGGCGAGTACGTGA
AGAGGCTGTCCGCGGCGGAGCAGCGCGCCATTCGCGCGATGATCAACCTGGAGTGCCTGGGTCTGGCCACGCCGAAAGTC
TGGGCGAGCCGGGCGGACCGGCATCTGCGGGACGCCTACGGACGCGTCGCTCAGTCGCTCGGCATGGTAGCCGAATCAGG
CAATGTAGATCAGGTGGGTGATGACGATTCCCACCCGTTTCTCAATGCGAAGATCCCGGTGCTCACCATCCATTCCGTCA
CCGCGAGGAATATGGGGATTCTGCACACGGCCCGCGACCAGGTGAGCGCGATTCACCCCGATGAGTATTACGCCACGTAC
CGCCTGGCAACCGCGTACCTCGAATACCTCGATACCTGGCTAGAGTAA

Upstream 100 bases:

>100_bases
GACCTCTATGAAAAACTCCTCGATATGACGGGCCCGGTTCCCCCCGAGATCGAAGGAGTGCTGTAGTTTTGCAATCGTGA
AAATCTCCTTCTTCCTCCTT

Downstream 100 bases:

>100_bases
AGTGGATCCAACGAGGCGGTCGCGGCTCGTGGTCAGAGCGTGATTTTTTCCCCGGTGCGGCAGGAGCGATAGACGGCATC
GACGATTTCGAGGGCCTGGT

Product: peptidase M28

Products: NA

Alternate protein names: LIE1 [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSWCAWAQPALEQPVIQHRLESLSRKIPIRRATLEVLFREAGCDGANLTAQRVPGSKEPNIICTLPGDSPSAIVVGGHYD
LVEEGAGAVDDWSGAVLLPSLYETLKRTPRKHTYVFIAFAAEEVGLHGSGEYVKRLSAAEQRAIRAMINLECLGLATPKV
WASRADRHLRDAYGRVAQSLGMVAESGNVDQVGDDDSHPFLNAKIPVLTIHSVTARNMGILHTARDQVSAIHPDEYYATY
RLATAYLEYLDTWLE

Sequences:

>Translated_255_residues
MSWCAWAQPALEQPVIQHRLESLSRKIPIRRATLEVLFREAGCDGANLTAQRVPGSKEPNIICTLPGDSPSAIVVGGHYD
LVEEGAGAVDDWSGAVLLPSLYETLKRTPRKHTYVFIAFAAEEVGLHGSGEYVKRLSAAEQRAIRAMINLECLGLATPKV
WASRADRHLRDAYGRVAQSLGMVAESGNVDQVGDDDSHPFLNAKIPVLTIHSVTARNMGILHTARDQVSAIHPDEYYATY
RLATAYLEYLDTWLE
>Mature_254_residues
SWCAWAQPALEQPVIQHRLESLSRKIPIRRATLEVLFREAGCDGANLTAQRVPGSKEPNIICTLPGDSPSAIVVGGHYDL
VEEGAGAVDDWSGAVLLPSLYETLKRTPRKHTYVFIAFAAEEVGLHGSGEYVKRLSAAEQRAIRAMINLECLGLATPKVW
ASRADRHLRDAYGRVAQSLGMVAESGNVDQVGDDDSHPFLNAKIPVLTIHSVTARNMGILHTARDQVSAIHPDEYYATYR
LATAYLEYLDTWLE

Specific function: A leucine-specific metalloprotease that plays a role in controlling the amount of leupeptin during colony development. Degrades leupeptin into three components, acetyl-leucine, leucine and argininal [H]

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28A subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR007484
- InterPro:   IPR002884 [H]

Pfam domain/function: PF01483 P_proprotein; PF04389 Peptidase_M28 [H]

EC number: NA

Molecular weight: Translated: 28063; Mature: 27932

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWCAWAQPALEQPVIQHRLESLSRKIPIRRATLEVLFREAGCDGANLTAQRVPGSKEPN
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
IICTLPGDSPSAIVVGGHYDLVEEGAGAVDDWSGAVLLPSLYETLKRTPRKHTYVFIAFA
EEEECCCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCCEEEEEEEE
AEEVGLHGSGEYVKRLSAAEQRAIRAMINLECLGLATPKVWASRADRHLRDAYGRVAQSL
EHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GMVAESGNVDQVGDDDSHPFLNAKIPVLTIHSVTARNMGILHTARDQVSAIHPDEYYATY
HHHHCCCCCCCCCCCCCCCEEECCCCEEEEECHHHCCCCEEEECHHHHHCCCCHHHHHHH
RLATAYLEYLDTWLE
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SWCAWAQPALEQPVIQHRLESLSRKIPIRRATLEVLFREAGCDGANLTAQRVPGSKEPN
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
IICTLPGDSPSAIVVGGHYDLVEEGAGAVDDWSGAVLLPSLYETLKRTPRKHTYVFIAFA
EEEECCCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCCEEEEEEEE
AEEVGLHGSGEYVKRLSAAEQRAIRAMINLECLGLATPKVWASRADRHLRDAYGRVAQSL
EHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GMVAESGNVDQVGDDDSHPFLNAKIPVLTIHSVTARNMGILHTARDQVSAIHPDEYYATY
HHHHCCCCCCCCCCCCCCCEEECCCCEEEEECHHHCCCCEEEECHHHHHCCCCHHHHHHH
RLATAYLEYLDTWLE
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA